##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294747_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4614787
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.101358307544857	33.0	33.0	33.0	27.0	33.0
2	31.023061519415737	33.0	33.0	33.0	27.0	33.0
3	31.1136197618655	33.0	33.0	33.0	27.0	33.0
4	34.471821993084404	37.0	37.0	37.0	27.0	37.0
5	34.870823507130446	37.0	37.0	37.0	33.0	37.0
6	34.94726842213953	37.0	37.0	37.0	33.0	37.0
7	34.961174372728365	37.0	37.0	37.0	33.0	37.0
8	34.88712956849363	37.0	37.0	37.0	33.0	37.0
9	34.55607550250965	37.0	37.0	37.0	27.0	37.0
10	34.6823006132244	37.0	37.0	37.0	27.0	37.0
11	34.94000914885129	37.0	37.0	37.0	33.0	37.0
12	34.88424102780908	37.0	37.0	37.0	33.0	37.0
13	34.740042173127385	37.0	37.0	37.0	27.0	37.0
14	36.01448907609387	40.0	37.0	40.0	27.0	40.0
15	36.239447671149286	40.0	37.0	40.0	27.0	40.0
16	36.216535671093816	40.0	37.0	40.0	27.0	40.0
17	36.22628975075123	40.0	37.0	40.0	27.0	40.0
18	36.206133024124405	40.0	37.0	40.0	27.0	40.0
19	36.19309991988796	40.0	37.0	40.0	27.0	40.0
20	36.1375478434866	40.0	37.0	40.0	27.0	40.0
21	36.05875244079521	40.0	37.0	40.0	27.0	40.0
22	35.98892126548853	40.0	37.0	40.0	27.0	40.0
23	35.65805702408367	37.0	37.0	40.0	27.0	40.0
24	36.000383983052735	40.0	37.0	40.0	27.0	40.0
25	34.936641279435	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5056653626531684
1101	2	0.6057599842974604
1101	3	0.45638837334829674
1101	4	0.6486507573004872
1101	5	0.5416848896419566
1101	6	0.4225683052090474
1101	7	0.38290598030016554
1101	8	0.5475149003118034
1101	9	0.7949352242466645
1101	10	0.73952782445037
1101	11	0.4581484724398095
1101	12	0.5787759120911389
1101	13	0.5922131576937062
1101	14	1.2576320952293756
1101	15	1.052226669614086
1101	16	0.9593538240464881
1101	17	1.0747187888528842
1101	18	1.0994007650556128
1101	19	1.0686389144163257
1101	20	1.1127684350394773
1101	21	1.0933800575438113
1101	22	1.1540702349672713
1101	23	1.4580709999376467
1101	24	1.2590965700868324
1101	25	1.0820443831618718
1102	1	0.3586934608282988
1102	2	0.5669150122408801
1102	3	0.4036423302313139
1102	4	0.5261807038098638
1102	5	0.3760951075232484
1102	6	0.3757758702751559
1102	7	0.3092054928191672
1102	8	0.45452384370359766
1102	9	0.669213550187493
1102	10	0.6628295659531531
1102	11	0.49452034580956905
1102	12	0.4446747067425747
1102	13	0.4626630230311406
1102	14	1.21801134695977
1102	15	0.9142338532818499
1102	16	0.6414765254849684
1102	17	0.8194988982303428
1102	18	0.857512593646014
1102	19	0.8328843172932849
1102	20	0.9464358247322409
1102	21	0.9353388675210752
1102	22	0.9192813644105726
1102	23	1.1646229142927496
1102	24	1.0315347987810668
1102	25	0.9064436433289558
1103	1	0.3629300884787554
1103	2	0.5007584694603828
1103	3	0.3338320608057792
1103	4	0.20944311142389438
1103	5	0.40646975503231175
1103	6	0.2716388167134838
1103	7	0.2829558090070492
1103	8	0.39463221528804837
1103	9	0.6319426386250768
1103	10	0.6457854888046484
1103	11	0.4595840718880311
1103	12	0.43022432939157795
1103	13	0.3466839246673743
1103	14	1.0167090766973104
1103	15	0.7990404622889358
1103	16	0.4696268609452545
1103	17	0.6029162880954715
1103	18	0.7032282534113463
1103	19	0.6459998129755107
1103	20	0.8365107595874974
1103	21	0.7959677100124409
1103	22	0.747023856537659
1103	23	1.265875129838534
1103	24	0.9697098626989771
1103	25	0.8454758831169258
1104	1	0.3117428952379129
1104	2	0.4758423929447595
1104	3	0.3571251906249735
1104	4	0.37532538249250536
1104	5	0.3357366002460225
1104	6	0.23376509167493253
1104	7	0.1753406106138442
1104	8	0.37107989942086306
1104	9	0.5755232250380757
1104	10	0.5674164943893771
1104	11	0.3627284774150752
1104	12	0.3858845278266685
1104	13	0.41764170592998795
1104	14	0.910166578197078
1104	15	0.6749657577689732
1104	16	0.5914314591080796
1104	17	0.6571595011347142
1104	18	0.6449211010835398
1104	19	0.7069769016333751
1104	20	0.7794523268479665
1104	21	0.7311001028127961
1104	22	0.8100396806975283
1104	23	1.1929947710375828
1104	24	1.0186063942625694
1104	25	0.8443519345365829
1105	1	0.3042835605787708
1105	2	0.3464842666473835
1105	3	0.3296681568085589
1105	4	0.4051333040585092
1105	5	0.32198129241321993
1105	6	0.2603166981328968
1105	7	0.27215125799228446
1105	8	0.412753405009326
1105	9	-1.5506428843703048
1105	10	-0.03771873920906188
1105	11	0.1612383174781229
1105	12	0.25774666990361794
1105	13	0.29021953631916375
1105	14	-1.6171096115836434
1105	15	-0.19139204589365733
1105	16	-0.01950770533105839
1105	17	0.2907236543438998
1105	18	0.33917387805288257
1105	19	0.43262493597794816
1105	20	0.5041122949504597
1105	21	0.627861730211734
1105	22	0.6912356750116686
1105	23	0.9108819086871449
1105	24	0.7389263172944638
1105	25	0.46248612475028494
1106	1	0.13821349620149803
1106	2	0.026445715187094265
1106	3	0.11315776816923417
1106	4	-0.9606687184732223
1106	5	-0.21462072993060133
1106	6	-0.04966460436188669
1106	7	0.06724271340365107
1106	8	0.21933323743768796
1106	9	0.3879958776338981
1106	10	0.45364916118490584
1106	11	0.2932617165413376
1106	12	0.2179207019416367
1106	13	0.19550266304526787
1106	14	0.5880119239084536
1106	15	0.37382209612154327
1106	16	0.1896286713244919
1106	17	0.10550595329874568
1106	18	0.2232209469286488
1106	19	0.19729112659837966
1106	20	0.3108308116813774
1106	21	0.4821131332719588
1106	22	0.5708230239797629
1106	23	0.7062590601675183
1106	24	0.43213052596909307
1106	25	0.38215795605312053
1107	1	0.2976137723701733
1107	2	0.23691137422984454
1107	3	0.25443182438761625
1107	4	0.41404644976229577
1107	5	0.3723072287353659
1107	6	0.20188523483589194
1107	7	0.22034650121175758
1107	8	0.31188692635738136
1107	9	0.47074288765763583
1107	10	0.41268084223148804
1107	11	0.23166775618636848
1107	12	0.37581447958424974
1107	13	0.32087268686976955
1107	14	0.6932162424870754
1107	15	0.5406501607235299
1107	16	0.5072344902122765
1107	17	0.5304068679913811
1107	18	0.552205673645652
1107	19	0.5254884010178813
1107	20	0.6463428152047683
1107	21	0.6299816102610691
1107	22	0.5497063481653655
1107	23	0.7537885055725084
1107	24	0.7628625355263523
1107	25	0.6694661212034418
1108	1	0.4967287751456553
1108	2	0.44895241593548363
1108	3	0.4826062237574611
1108	4	0.6350718143141236
1108	5	0.606353531227029
1108	6	0.4447297443029328
1108	7	0.37876668543012926
1108	8	0.45048536386395455
1108	9	0.34981099229415946
1108	10	0.6069205847221042
1108	11	0.3990969862481535
1108	12	0.3654129838476976
1108	13	0.38786452550711203
1108	14	-0.9988463699140908
1108	15	0.07437629801009393
1108	16	0.36470146181822827
1108	17	0.4350984464490679
1108	18	0.5909182529744328
1108	19	0.5387552299668741
1108	20	0.6510361916986227
1108	21	0.73513277345657
1108	22	0.8647983655351581
1108	23	1.0989896426964805
1108	24	0.9709592788620469
1108	25	0.9045780470417242
1109	1	0.33988541371763503
1109	2	0.24084971082095308
1109	3	0.3596344066035897
1109	4	0.4098985010179632
1109	5	0.43919079468532374
1109	6	0.24055403334531889
1109	7	0.21315310363737439
1109	8	0.23809873322271358
1109	9	0.4096933123115676
1109	10	0.42995962782411823
1109	11	0.28865954316212594
1109	12	0.28327507192790335
1109	13	0.2363121151015406
1109	14	0.63864949809102
1109	15	0.3654558372549701
1109	16	0.29776907978968126
1109	17	0.29636268226386875
1109	18	0.37776321053853223
1109	19	0.3768915307688019
1109	20	0.499030908891541
1109	21	0.5259654013976345
1109	22	0.6347728753763633
1109	23	1.0024928252689023
1109	24	0.7623137146125742
1109	25	0.639050783366379
1110	1	0.32026483361506664
1110	2	0.2489263105596322
1110	3	0.34809391500306575
1110	4	0.4905260732775716
1110	5	0.4188004358266255
1110	6	0.23077232864550012
1110	7	0.2451948067391143
1110	8	0.18306360362640817
1110	9	0.38298536569639907
1110	10	0.37024561638772724
1110	11	0.1729760887912093
1110	12	0.1449259097375446
1110	13	0.16516576372904979
1110	14	0.5484327783613878
1110	15	0.34904300625854034
1110	16	0.30179004838293366
1110	17	0.3507933554714029
1110	18	0.4302409640450193
1110	19	0.3840387689624336
1110	20	0.485161273849954
1110	21	0.44697934857325095
1110	22	0.5945778197418434
1110	23	0.7155863792721462
1110	24	0.6423686795167427
1110	25	0.6001727921738222
1111	1	0.1369731148778719
1111	2	0.11949971543013405
1111	3	0.2863265100251233
1111	4	0.5506763048247976
1111	5	0.333621287927258
1111	6	0.2687056433783894
1111	7	0.2043419779413398
1111	8	0.26848480469825375
1111	9	0.2842627153861983
1111	10	0.2547382061962722
1111	11	0.15458035684151383
1111	12	0.1738668854624308
1111	13	0.12479615017139167
1111	14	-1.8971721634369132
1111	15	-0.4965831911181269
1111	16	-0.05694266450137775
1111	17	0.13107838099282532
1111	18	0.18273244771967256
1111	19	0.26429761055014467
1111	20	0.3115100910574
1111	21	0.427993887076056
1111	22	0.183926519941771
1111	23	-1.7280140494620326
1111	24	-0.340892588894981
1111	25	0.08408491265090134
1112	1	-0.1802905611958856
1112	2	-0.21735217120846428
1112	3	-0.013355781083060236
1112	4	0.19290969373984268
1112	5	-0.646599421261655
1112	6	-0.8219939616656475
1112	7	-0.8816700926605208
1112	8	-0.7252796807748467
1112	9	-0.5871673452302772
1112	10	-1.0035693539615522
1112	11	-0.8524524465554606
1112	12	-0.7159996701286317
1112	13	-0.8308251947865912
1112	14	-0.5993057581948662
1112	15	-0.917511334565134
1112	16	-0.764990595977352
1112	17	-0.8657793422084907
1112	18	-0.9181626821754634
1112	19	-0.8104720248157378
1112	20	-0.9263240435957769
1112	21	-1.8362312416010056
1112	22	-1.5902592571645897
1112	23	-1.3150592865115769
1112	24	-1.6399895024457933
1112	25	-1.89732678553856
1113	1	0.01634066898328257
1113	2	-0.11069482777791606
1113	3	-0.04152615059648568
1113	4	0.11897394011898399
1113	5	0.07874563573396642
1113	6	-0.05105845068098347
1113	7	-0.017939767606506507
1113	8	-0.02331468590910646
1113	9	0.22747202947133616
1113	10	-0.04503610171170891
1113	11	-0.12466174638111482
1113	12	-0.06015944077429225
1113	13	-0.09928694272955596
1113	14	-0.05984174871873904
1113	15	-0.14043606990910718
1113	16	-0.04899520572381988
1113	17	-0.050774665233710436
1113	18	0.058750347251766755
1113	19	-0.19754514635599918
1113	20	-0.6744033443394173
1113	21	-0.4709239348388934
1113	22	-0.10448807845433095
1113	23	-0.2941488240456209
1113	24	0.05286243862671114
1113	25	-1.1675904911563535
1114	1	-0.9590175972629815
1114	2	-0.7448199538103601
1114	3	-0.583016825408265
1114	4	-1.5621682923756381
1114	5	-0.8102826305607991
1114	6	-0.7142531514714818
1114	7	-0.5194504055260154
1114	8	-0.45253012418945104
1114	9	-0.5447134525613251
1114	10	-0.5664151377811066
1114	11	-0.5495869621923077
1114	12	-0.6085981339061135
1114	13	-0.6371029399445831
1114	14	-0.5836630254199733
1114	15	-1.2890690725986929
1114	16	-0.9120066306893193
1114	17	-0.8432191045287851
1114	18	-0.8873399660739096
1114	19	-0.8765662481341678
1114	20	-1.9491092731318247
1114	21	-6.685488475574253
1114	22	-7.620751046121587
1114	23	-7.886106970766516
1114	24	-5.850700648727578
1114	25	-7.1545308761272
1115	1	-1.123051382221444
1115	2	-0.9317866136492405
1115	3	-0.8437454002767453
1115	4	-0.7150817288099347
1115	5	-0.7599463286385557
1115	6	-0.8912364033854132
1115	7	-1.0105402475358218
1115	8	-0.6945537847526495
1115	9	-2.6634896955700498
1115	10	-1.6012813507341974
1115	11	-1.2004183501135373
1115	12	-1.0797893630169924
1115	13	-1.393994342451741
1115	14	-1.047680012916544
1115	15	-1.9880662316216302
1115	16	-1.3804583140937439
1115	17	-3.0971888101783307
1115	18	-2.8773805026577364
1115	19	-2.135845843097499
1115	20	-1.7949176148523662
1115	21	-1.278252216817826
1115	22	-0.9298051546950177
1115	23	-0.4048611053002844
1115	24	-1.4311845394153124
1115	25	-1.0961153166015976
1116	1	-1.5264673425429898
1116	2	-0.8979891109210278
1116	3	-0.6637905434688065
1116	4	-0.518837585394472
1116	5	-0.4143884202484145
1116	6	-0.4797985688625488
1116	7	-0.9375432036733216
1116	8	-1.8626444038942864
1116	9	-0.8910202422102742
1116	10	-2.5601155789470624
1116	11	-1.2293752348065468
1116	12	-0.9343082644753196
1116	13	-0.8601788309498559
1116	14	-1.2797590252686248
1116	15	-1.435755709557533
1116	16	-0.9667468763923566
1116	17	-0.7606465508547942
1116	18	-1.340036692787784
1116	19	-0.9870672679322396
1116	20	-1.2711877902110444
1116	21	-0.7289616635512388
1116	22	-0.3139175087173527
1116	23	-0.18742308201812818
1116	24	-0.3665544329699699
1116	25	-0.2591272636688302
1201	1	0.32335946969134355
1201	2	0.5048770008010912
1201	3	0.45773197539020316
1201	4	0.5550685078559852
1201	5	0.4230665943943208
1201	6	0.32694086415708057
1201	7	0.3558257635771298
1201	8	0.4964313517095462
1201	9	0.734507636038181
1201	10	0.6719627012556018
1201	11	0.4822003535447976
1201	12	0.50735494193205
1201	13	0.5220762124873204
1201	14	1.196100322778797
1201	15	0.8769830026475702
1201	16	0.8441918653381819
1201	17	0.785031017178035
1201	18	0.7422289260917125
1201	19	0.7046603763693113
1201	20	0.9364773873125714
1201	21	0.9667007047662324
1201	22	0.9694746291603522
1201	23	0.8339545270610884
1201	24	0.8322272141007474
1201	25	0.8009309195385086
1202	1	-0.1559037455705763
1202	2	0.033999699754300394
1202	3	0.094219464730255
1202	4	0.0453697552852077
1202	5	0.07897759089353684
1202	6	-0.008793322136128268
1202	7	0.03608896724355759
1202	8	0.26735287375521466
1202	9	0.2715747419749661
1202	10	0.3796852653496927
1202	11	0.19209127508089807
1202	12	0.16006435216763748
1202	13	0.27330480781967736
1202	14	0.4552482015236592
1202	15	0.4679847936243817
1202	16	0.4237097246834338
1202	17	0.240265820667787
1202	18	0.22470523526694564
1202	19	0.29966805926275697
1202	20	0.4012933193812458
1202	21	0.48191531101311114
1202	22	0.3756611943395072
1202	23	0.7941656423614134
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2211	8	0.29580128050834276
2211	9	0.43808482450307906
2211	10	0.39463453498609624
2211	11	0.1885046265110688
2211	12	0.3053511043429751
2211	13	0.1237463155785008
2211	14	0.5474203949526526
2211	15	0.30679566146671533
2211	16	0.26877991230328035
2211	17	0.2799165383302977
2211	18	0.2819490187604927
2211	19	0.2964013066849347
2211	20	0.3277902411678255
2211	21	0.35306762253718915
2211	22	0.4078830661028121
2211	23	0.6194259853519313
2211	24	0.5960837967462567
2211	25	0.6591145560829546
2212	1	-0.29939036956950815
2212	2	-0.3246866296705555
2212	3	-0.25382851240419413
2212	4	-0.5893756211143497
2212	5	-0.21013653509407249
2212	6	-0.6082758468526634
2212	7	-0.43652490138586586
2212	8	-0.2508191803824147
2212	9	-0.26341029032771957
2212	10	-0.5734029238968716
2212	11	-0.5668455278444497
2212	12	-0.7415802496751809
2212	13	-0.6322327873563083
2212	14	-0.5008158752145349
2212	15	-0.7505852642388646
2212	16	-0.40903370018801866
2212	17	-0.47693807527732446
2212	18	-0.528061008864924
2212	19	-0.5938659441354801
2212	20	-0.5924731030313382
2212	21	-0.523876925373358
2212	22	-0.6365771560724909
2212	23	-0.1342579636821526
2212	24	-0.4033186687039674
2212	25	-1.125298845271928
2213	1	-0.06699231734985744
2213	2	-1.2637152640968559
2213	3	-0.31169770279081277
2213	4	0.017978851022434128
2213	5	0.06702294499059036
2213	6	-0.03659000337498952
2213	7	-0.013918550268492424
2213	8	0.13264810496912105
2213	9	0.133611068463928
2213	10	-0.3563997278485118
2213	11	-0.05383728869708193
2213	12	-0.03324425324072422
2213	13	-0.410420675641447
2213	14	0.033934605806024365
2213	15	-0.08143462520178701
2213	16	-0.11961702089168114
2213	17	-6.502186514509845E-4
2213	18	-0.060573958076794554
2213	19	-0.018609504302773416
2213	20	0.05214994773761106
2213	21	-0.5347709014404387
2213	22	-0.8809031155801037
2213	23	-0.16579728070181687
2213	24	0.10863634498676333
2213	25	-0.5081940438159762
2214	1	-0.2699659126202043
2214	2	-0.5116103025650496
2214	3	-0.5116499003245494
2214	4	-0.6492343702368331
2214	5	-0.2833209746061698
2214	6	-0.34238888673940693
2214	7	-0.5050550734140415
2214	8	-0.25380867296750154
2214	9	-0.15451418907898073
2214	10	-1.9877183745378701
2214	11	-0.9485283435671477
2214	12	-0.6064669330462564
2214	13	-0.42359875561754023
2214	14	-0.979196995434009
2214	15	-0.6955403667465561
2214	16	-0.849972403596901
2214	17	-0.683412264711599
2214	18	-0.6697224617051631
2214	19	-1.5554694799237438
2214	20	-2.587959403573336
2214	21	-1.9872679044428097
2214	22	-2.1026212080677524
2214	23	-2.6541790784762753
2214	24	-1.782230677225499
2214	25	-2.028865325844727
2215	1	-0.3612865224940016
2215	2	-0.451850955125888
2215	3	-0.8823918692101707
2215	4	-0.40958640087168874
2215	5	-0.31360060803899614
2215	6	-0.2562803757923291
2215	7	-0.2711524605642097
2215	8	-0.2599740136165991
2215	9	-0.25886851584738935
2215	10	-0.31633662872032886
2215	11	-0.6631864969692742
2215	12	-0.5120917068628259
2215	13	-0.39905952216028595
2215	14	-0.31013438151620676
2215	15	-0.6907770659713037
2215	16	-0.5343080990628764
2215	17	-0.33873726700482365
2215	18	-0.35948008294675304
2215	19	-2.242783130551402
2215	20	-2.5315058698777264
2215	21	-3.3425414380273963
2215	22	-2.3525596808878078
2215	23	-3.452153955296353
2215	24	-2.4150069924201816
2215	25	-1.7711479273857265
2216	1	-1.50911792737487
2216	2	-1.1797932211961246
2216	3	-0.9008950436341685
2216	4	-1.0044680340266154
2216	5	-2.016124909353586
2216	6	-1.5371398479365865
2216	7	-1.2643825924561156
2216	8	-5.288704307592788
2216	9	-4.25574380265677
2216	10	-2.171957960730566
2216	11	-1.130727943102002
2216	12	-1.27458946057682
2216	13	-2.0121847006711278
2216	14	-3.5469476934889954
2216	15	-2.1362562805297074
2216	16	-3.2020197353891504
2216	17	-2.791059985919553
2216	18	-2.782002744819181
2216	19	-1.8483677036170576
2216	20	-1.066408014320828
2216	21	-0.8497914888710696
2216	22	-0.8457464595442445
2216	23	-1.0867459347313613
2216	24	-0.6930280281404748
2216	25	-0.5393989800881798
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	75.0
8	351.0
9	913.0
10	1800.0
11	3499.0
12	5845.0
13	9014.0
14	13013.0
15	19057.0
16	24518.0
17	26829.0
18	27224.0
19	27817.0
20	28380.0
21	30469.0
22	31622.0
23	32978.0
24	34879.0
25	36989.0
26	39202.0
27	43414.0
28	49051.0
29	58175.0
30	69258.0
31	85290.0
32	114600.0
33	165080.0
34	242544.0
35	390044.0
36	693689.0
37	2309166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05351276773995	19.400483592849934	11.50183650399869	24.044167135411417
2	12.686340246459041	22.54095242966464	37.42675939457395	27.345947929302376
3	20.75532304339128	28.67998580767404	27.494900581109427	23.06979056782525
4	12.860780543123735	19.308662547836747	33.88129203868209	33.94926487035743
5	11.66962207415201	40.303369814787956	34.85362875703603	13.173379354024004
6	32.41062082685894	33.52683921609421	18.332282259829704	15.730257697217148
7	24.480962015913256	33.63144234559423	23.03510802460775	18.852487613884758
8	26.319334228448543	36.546812820661735	21.352571664836276	15.781281286053453
9	26.369859822552126	17.802516376644732	20.71415584840544	35.1134679523977
10	16.014595936601854	32.17581000232084	32.17607004038608	19.63352402069123
11	29.099205066218474	27.91898280373864	19.906780990585784	23.075031139457096
12	22.87013055681241	31.1209665815473	28.994304283532436	17.014598578107854
13	28.1169909179027	22.84291250341573	23.41261577447465	25.62748080420692
14	20.850001744400096	26.61352133271301	26.17449591712864	26.361981005758256
15	30.160021063101546	25.847719410016456	21.60642988013318	22.385829646748817
16	19.135810628812425	29.628644047705986	30.780749186888894	20.454796136592698
17	24.75706906168697	30.253521041301617	27.424011081143984	17.56539881586743
18	25.024248659931068	26.840774500898	28.56225599650507	19.572720842665852
19	22.525011008137355	31.21368181455709	29.19260513204564	17.068702045259915
20	23.21639037548456	28.12774323761793	27.517743621170087	21.138122765727427
21	19.472259693167178	30.23532958286525	30.011906362638747	20.280504361328827
22	21.40151556225052	29.435010433532693	23.52257403557594	25.640899968640845
23	18.09721120099899	30.753214070625518	27.671249929246095	23.478324799129396
24	20.75335587849191	34.61063228291617	24.923343427080802	19.71266841151112
25	21.864956657175867	27.13171088334828	22.890323791345423	28.113008668130433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10342.0
1	10342.0
2	12935.0
3	15528.0
4	15528.0
5	15528.0
6	19565.0
7	23602.0
8	23602.0
9	23602.0
10	25625.0
11	27648.0
12	27648.0
13	27648.0
14	34446.0
15	41244.0
16	41244.0
17	41244.0
18	56939.5
19	72635.0
20	72635.0
21	72635.0
22	98019.5
23	123404.0
24	123404.0
25	123404.0
26	156630.5
27	189857.0
28	189857.0
29	189857.0
30	225143.5
31	260430.0
32	260430.0
33	260430.0
34	291984.0
35	323538.0
36	323538.0
37	323538.0
38	351245.0
39	378952.0
40	378952.0
41	378952.0
42	427469.5
43	475987.0
44	475987.0
45	475987.0
46	779902.0
47	1083817.0
48	1083817.0
49	1083817.0
50	930970.5
51	778124.0
52	778124.0
53	778124.0
54	569103.5
55	360083.0
56	360083.0
57	360083.0
58	278240.0
59	196397.0
60	196397.0
61	196397.0
62	161401.0
63	126405.0
64	126405.0
65	126405.0
66	98364.0
67	70323.0
68	70323.0
69	70323.0
70	52318.5
71	34314.0
72	34314.0
73	34314.0
74	24394.5
75	14475.0
76	14475.0
77	14475.0
78	9769.5
79	5064.0
80	5064.0
81	5064.0
82	3430.5
83	1797.0
84	1797.0
85	1797.0
86	1199.0
87	601.0
88	601.0
89	601.0
90	380.5
91	160.0
92	160.0
93	160.0
94	97.5
95	35.0
96	35.0
97	35.0
98	30.0
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03038060044808135
2	0.015472003366569247
3	0.022232878787254967
4	0.025548308080091238
5	0.02622006172765937
6	0.009902948933504406
7	0.0031204040403164874
8	0.002405311447743959
9	0.0029470482602989042
10	0.0016902188551714303
11	0.001365176767638463
12	0.0019719219977000022
13	0.002253625140228574
14	4.3338945004395654E-4
15	0.0017768967451802218
16	0.002253625140228574
17	0.0021886167227219804
18	0.002145277777717585
19	4.117199775417587E-4
20	6.500841750659348E-4
21	0.08249568181586713
22	0.08002536195061656
23	0.08091381032320669
24	0.0010618041526076936
25	0.23142996632347276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4614787.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.56078683571746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13499175891839	48.9684948988498
2	8.660134713343457	9.623277975533458
3	1.682494095091537	2.804420873092027
4	0.5447579843842155	1.21068728989706
5	0.24943098564136168	0.6929290911721299
6	0.1478391997878001	0.4928437359223804
7	0.09227352482512285	0.3588752750372254
8	0.07048447147084967	0.313293815569607
9	0.05475561351765279	0.2738038473641913
>10	0.30923809005408454	3.1938834023173555
>50	0.027443287452417975	1.053096862185199
>100	0.019384603799214774	2.1979966566637787
>500	0.00225722391346492	0.8974079997502706
>1k	0.0028908306090324226	3.5181024913238947
>5k	3.9600419301814003E-4	1.60079967015492
>10k+	0.0012276129983562342	22.80008611516669
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	167140	3.6218356340173443	No Hit
CCCATGTACTCTGCGTTGATACCAC	159038	3.4462695678045376	No Hit
GAGTACATGGGAAGCAGTGGTATCA	75192	1.6293709763852589	No Hit
CATGTACTCTGCGTTGATACCACTG	58982	1.2781088271246321	No Hit
GCGTTGATACCACTGCTTCCCATGT	51434	1.114547648678043	No Hit
GCTTCCCATGTACTCTGCGTTGATA	44948	0.9739994500287878	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	42862	0.9287969303892033	No Hit
GTATCAACGCAGAGTACATGGGAAG	41325	0.8954909511533252	No Hit
ACTCTGCGTTGATACCACTGCTTCC	34483	0.7472284202932876	No Hit
TATCAACGCAGAGTACATGGGAAGC	33454	0.7249305330885261	No Hit
GGTATCAACGCAGAGTACATGGGAA	27924	0.6050983501513721	No Hit
CAGTGGTATCAACGCAGAGTACATG	21875	0.47401971098557744	No Hit
GTGGTATCAACGCAGAGTACATGGG	21290	0.4613430695717917	No Hit
GCAGTGGTATCAACGCAGAGTACAT	18281	0.39613962681267845	No Hit
ATACCACTGCTTCCCATGTACTCTG	17704	0.3836363411789103	No Hit
GTACTCTGCGTTGATACCACTGCTT	16801	0.36406880750942566	No Hit
CATGGGAAGCAGTGGTATCAACGCA	16007	0.3468632463426806	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	15889	0.34430624858742126	No Hit
GTACATGGTAAGCAGTGGTATCAAC	15773	0.3417925897771663	No Hit
ACATGGGAAGCAGTGGTATCAACGC	15560	0.33717699213419816	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	15493	0.3357251374765509	No Hit
TACCACTGCTTCCCATGTACTCTGC	14065	0.3047811307434124	No Hit
GATACCACTGCTTCCCATGTACTCT	13445	0.29134605779204975	No Hit
CTTCCCATGTACTCTGCGTTGATAC	13363	0.2895691610468696	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	12807	0.27752093433564756	No Hit
GTTGATACCACTGCTTCCCATGTAC	12788	0.27710921435810576	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	12504	0.2709550841674816	No Hit
CCACTGCTTCCCATGTACTCTGCGT	12380	0.26826806957720906	No Hit
CCATGTACTCTGCGTTGATACCACT	11723	0.2540312261432651	No Hit
ACCATGTACTCTGCGTTGATACCAC	11396	0.24694530863504643	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10333	0.22391065936521012	No Hit
TTCCCATGTACTCTGCGTTGATACC	9596	0.20794025813109032	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9455	0.20488486250828045	No Hit
TGATACCACTGCTTCCCATGTACTC	8799	0.1906696885468387	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7489	0.1622826795689595	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7290	0.15797045454102215	No Hit
CCCCATGTACTCTGCGTTGATACCA	7176	0.1555001346757716	No Hit
ATCAACGCAGAGTACATGGGAAGCA	6863	0.14871758978258368	No Hit
GCTTACCATGTACTCTGCGTTGATA	5944	0.12880334455306386	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	5096	0.11042763187120012	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5048	0.10938749719109464	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4980	0.10791397306094519	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4909	0.10637544051328914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.333894500439565E-5	0.0
10	0.0	0.0	0.0	1.0834736251098913E-4	0.0
11	0.0	0.0	0.0	1.3001683501318696E-4	0.0
12	0.0	0.0	0.0	2.1669472502197827E-4	0.0
13	0.0	0.0	0.0	2.1669472502197827E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	65	8.0148526E-4	11.69471	1
GTACAAA	1105	0.0	11.436739	1
ACCGTCT	85	5.3509146E-5	11.1717415	8
CGCAAAA	335	0.0	10.492304	2
CTAGGAC	290	0.0	9.826835	3
GTCTCGC	185	6.475602E-10	9.758761	1
TAGGACC	185	6.511982E-10	9.755693	4
GAACAAA	2065	0.0	9.663001	1
AGATCGA	80	0.004534098	9.495979	8
CTTACAC	460	0.0	9.292768	3
CTAGTAC	175	2.6775524E-8	9.227865	3
GATACGA	125	2.739846E-5	9.1247425	18
TGTGCGC	95	0.0018319166	8.996191	8
TTAACGT	85	0.0074460395	8.940203	4
CCGTCTG	85	0.007465922	8.937296	9
ATACGAC	140	1.0549524E-5	8.84184	19
CTCTTAG	325	0.0	8.771032	1
CTAAGAC	295	0.0	8.694251	3
CTAGACT	295	0.0	8.693969	4
GTCCTAG	330	0.0	8.638138	1
>>END_MODULE