FastQCFastQC Report
Wed 25 May 2016
SRR1294747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614787
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1748563.7890372838443027No Hit
GTACATGGGAAGCAGTGGTATCAAC1600073.4672672866591676No Hit
GAGTACATGGGAAGCAGTGGTATCA799811.7331460801982845No Hit
CATGTACTCTGCGTTGATACCACTG667301.446003900071661No Hit
GCGTTGATACCACTGCTTCCCATGT556301.205472755297265No Hit
ACGCAGAGTACATGGGAAGCAGTGG524101.135697053840188No Hit
GCTTCCCATGTACTCTGCGTTGATA507751.1002674662990946No Hit
GTATCAACGCAGAGTACATGGGAAG441130.9559054404894527No Hit
ACTCTGCGTTGATACCACTGCTTCC398830.864243571805156No Hit
TATCAACGCAGAGTACATGGGAAGC390430.8460412149033097No Hit
GGTATCAACGCAGAGTACATGGGAA308350.66817818460527No Hit
CAGTGGTATCAACGCAGAGTACATG264770.5737426234406918No Hit
GTGGTATCAACGCAGAGTACATGGG227940.4939339562150973No Hit
GCAGTGGTATCAACGCAGAGTACAT205760.4458710662052225No Hit
CATGGGAAGCAGTGGTATCAACGCA205460.4452209820301566No Hit
ACATGGGAAGCAGTGGTATCAACGC195380.42337815374794113No Hit
ATACCACTGCTTCCCATGTACTCTG193610.4195426571150521No Hit
GGGAAGCAGTGGTATCAACGCAGAG177300.38419974746396746No Hit
CTGCTTCCCATGTACTCTGCGTTGA171100.3707646745126048No Hit
GTACTCTGCGTTGATACCACTGCTT161930.3508937682280894No Hit
CTTCCCATGTACTCTGCGTTGATAC160240.34723162737521795No Hit
GATACCACTGCTTCCCATGTACTCT153670.332994783941274No Hit
TACCACTGCTTCCCATGTACTCTGC152840.3311962177235916No Hit
AAGCAGTGGTATCAACGCAGAGTAC148190.3211199130100696No Hit
GTACATGGTAAGCAGTGGTATCAAC147070.31869293208982347No Hit
GCAGAGTACATGGGAAGCAGTGGTA146350.3171327300696652No Hit
CCACTGCTTCCCATGTACTCTGCGT139300.3018557519556157No Hit
CCATGTACTCTGCGTTGATACCACT138870.30092396463802124No Hit
GTTGATACCACTGCTTCCCATGTAC131740.28547363074395415No Hit
ACCATGTACTCTGCGTTGATACCAC127530.2763507828205289No Hit
ATGGGAAGCAGTGGTATCAACGCAG125160.27121511783750796No Hit
GTATCAACGCAGAGTACTTTTTTTT115690.2506941273779267No Hit
TTCCCATGTACTCTGCGTTGATACC102170.22139700055495518No Hit
GTACATGGGTAAGCAGTGGTATCAA97420.21110400111641123No Hit
TGATACCACTGCTTCCCATGTACTC92540.20052929853533866No Hit
TATCAACGCAGAGTACTTTTTTTTT91990.1993374775477178No Hit
GGTAAGCAGTGGTATCAACGCAGAG85830.18598908248636395No Hit
GGTATCAACGCAGAGTACTTTTTTT85720.18575071828883977No Hit
ATCAACGCAGAGTACATGGGAAGCA83730.1814384932609024No Hit
CCCCATGTACTCTGCGTTGATACCA82340.1784264365830969No Hit
GTACTTTTTTTTTTTTTTTTTTTTT70720.15324650953554303No Hit
GCTTACCATGTACTCTGCGTTGATA65450.14182669752688476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA64680.14015814814421554No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62930.1363659904563309No Hit
GGAAGCAGTGGTATCAACGCAGAGT60820.13179373175836717No Hit
GAGTACATGGTAAGCAGTGGTATCA55670.1206339534197353No Hit
AAAAAGTACTCTGCGTTGATACCAC55410.12007054713467816No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47640.10323336700047044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG853.872514E-612.3153845
TAGACGG752.0424517E-411.4197215
TATCTCG600.005803490511.1025055
GTAGACG700.001471003310.8759233
TAACCCG909.35806E-510.5738145
AGTACTC14750.010.4519465
ACGTACT1701.5170372E-910.0762234
TACACTG7900.09.8778165
TAGACTG4550.09.8301615
GTCTAGG3200.09.5217081
CTAGGAC3000.09.5164343
ACGACAG900.00109691149.5164335
AAGTACT16050.09.4274954
CCAGGAC5150.09.424043
GTATTAG4100.09.289471
CTAAGAC3200.09.2190453
GTATTAA6200.09.2145561
GTTAGAC1252.7010936E-59.1357753
AAAAGTA13150.09.11850552
ACCGTCT1151.1198122E-49.080788
Produced by FastQC (version 0.11.5)