FastQCFastQC Report
Wed 25 May 2016
SRR1294746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437505
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC134720.39191215721868045No Hit
CCCATGTACTCTGCGTTGATACCAC117720.3424576836979146No Hit
GAGTACATGGGAAGCAGTGGTATCA61820.17983973841492595No Hit
GTATCAACGCAGAGTACTTTTTTTT50690.1474616036922128No Hit
GCGTTGATACCACTGCTTCCCATGT43840.1275343599500219No Hit
CATGTACTCTGCGTTGATACCACTG41500.12072709712422236No Hit
GTATCAACGCAGAGTACATGGGAAG36210.10533802859923112No Hit
GGTATCAACGCAGAGTACTTTTTTT35520.10333075879162357No Hit
ACGCAGAGTACATGGGAAGCAGTGG35400.10298166839030053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT551.9639048E-413.815974
GTCTAGG2600.010.2324071
ACTATCG2800.010.17442611
GTCTCGC1803.929017E-1010.0293831
TACACCG1252.5800473E-69.8777015
CTATCGG2950.09.65708312
GTCCTAG4100.09.5015211
CTAGACA3600.09.498484
TATACCG1002.7634724E-49.4977885
CGTGTTA900.00111839549.49613110
ACCGTTC1106.8717236E-59.4961318
GTGTAGG5150.09.4092731
GTCCTAA3250.09.3553441
TATCGGT3400.09.21669913
CCCGTAC1651.05897925E-79.21654716
TAGTACG950.00182728738.9985594
CTGACGC950.00183140368.9963349
CGGTACC850.0074483548.9397464
GTGTTAC3200.08.9076761
CTAGGAC3000.08.8658923
Produced by FastQC (version 0.11.5)