##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294746_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3437505
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991557248644003	33.0	33.0	33.0	27.0	33.0
2	31.035600239126925	33.0	33.0	33.0	27.0	33.0
3	30.99442764446888	33.0	33.0	33.0	27.0	33.0
4	34.47664657942316	37.0	37.0	37.0	27.0	37.0
5	34.68733165479032	37.0	37.0	37.0	27.0	37.0
6	34.84751702179342	37.0	37.0	37.0	33.0	37.0
7	34.820616115467466	37.0	37.0	37.0	33.0	37.0
8	34.74549447928076	37.0	37.0	37.0	27.0	37.0
9	34.46301285379948	37.0	37.0	37.0	27.0	37.0
10	34.54424095383134	37.0	37.0	37.0	27.0	37.0
11	34.76226245489097	37.0	37.0	37.0	27.0	37.0
12	34.69249499273456	37.0	37.0	37.0	27.0	37.0
13	34.608660641948156	37.0	37.0	37.0	27.0	37.0
14	35.73870205279702	37.0	37.0	40.0	27.0	40.0
15	36.04486306201736	40.0	37.0	40.0	27.0	40.0
16	36.065630159083405	40.0	37.0	40.0	27.0	40.0
17	36.014033143224516	40.0	37.0	40.0	27.0	40.0
18	36.01383503442177	40.0	37.0	40.0	27.0	40.0
19	36.105102683487004	40.0	37.0	40.0	27.0	40.0
20	35.9927965195687	40.0	37.0	40.0	27.0	40.0
21	35.94351135489257	40.0	37.0	40.0	27.0	40.0
22	35.80857046026115	37.0	37.0	40.0	27.0	40.0
23	35.43022250149454	37.0	33.0	40.0	27.0	40.0
24	35.74476022580331	37.0	37.0	40.0	27.0	40.0
25	34.53322104258758	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4853578602909785
1101	2	0.5919170066909558
1101	3	0.36077375763688124
1101	4	0.5588406949311846
1101	5	0.5334961858519449
1101	6	0.470830817635715
1101	7	0.4703087140146991
1101	8	0.5851111439248697
1101	9	0.7586083702933877
1101	10	0.8128357414859977
1101	11	0.6238928261921401
1101	12	0.6500211702560961
1101	13	0.6268258413843668
1101	14	1.1894199216007664
1101	15	0.9723244749799278
1101	16	0.9021263178176611
1101	17	0.9754650022233733
1101	18	0.9388631420548279
1101	19	0.8908441018565085
1101	20	1.0475811700047117
1101	21	1.0550832223072035
1101	22	0.9569933841560712
1101	23	1.412006071017231
1101	24	1.1144390409103337
1101	25	0.9808694784983913
1102	1	0.3420720752306323
1102	2	0.5855645082979279
1102	3	0.45714387212697005
1102	4	0.45031440700253
1102	5	0.46912299218055864
1102	6	0.3935685743513062
1102	7	0.4393255961885103
1102	8	0.43239906967549047
1102	9	0.6235097560219245
1102	10	0.6780975155248967
1102	11	0.4736069451497542
1102	12	0.49021456921270357
1102	13	0.46690196774326864
1102	14	1.1853957341274892
1102	15	0.9303672946025401
1102	16	0.5801111991925865
1102	17	0.8126905590403766
1102	18	0.8390965820025542
1102	19	0.8445310045743568
1102	20	0.9701962636162804
1102	21	0.9642432243625834
1102	22	1.0158304890663814
1102	23	1.3268987020205927
1102	24	1.0935846456601794
1102	25	0.8325096369947929
1103	1	0.3885176721152206
1103	2	0.586483844706418
1103	3	0.33149153537835474
1103	4	0.1599144712062639
1103	5	0.30981008684668154
1103	6	0.27539318349193564
1103	7	0.30913562190821864
1103	8	0.35589706521944464
1103	9	0.6945700349260235
1103	10	0.6329397386558213
1103	11	0.4610263751886947
1103	12	0.4287704436638151
1103	13	0.43126159276285136
1103	14	1.1252780272710652
1103	15	0.854434372224091
1103	16	0.5403796319716534
1103	17	0.6661173954393078
1103	18	0.6450193005723364
1103	19	0.7010627032600922
1103	20	0.8459917594181974
1103	21	0.9660922156377509
1103	22	0.7716794115518297
1103	23	1.2369670331437348
1103	24	1.0177460034164127
1103	25	0.8671335291936515
1104	1	0.23158013188755433
1104	2	0.4124199423695103
1104	3	0.27280569278458344
1104	4	0.23029918813112715
1104	5	0.2014206412654218
1104	6	0.0986215066540268
1104	7	0.17299107471521324
1104	8	0.3361490529881337
1104	9	0.462543224189119
1104	10	0.5663749795803241
1104	11	0.29199748274970716
1104	12	0.2616992101674853
1104	13	0.29298150637116294
1104	14	0.8535680010718352
1104	15	0.6380322658335302
1104	16	0.5132784599292322
1104	17	0.6057665065486191
1104	18	0.5698090654820689
1104	19	0.603688092696963
1104	20	0.7131158839454912
1104	21	0.8034734698670079
1104	22	0.8164315009878251
1104	23	1.1704326841584418
1104	24	0.9821982598751404
1104	25	0.7227305776278428
1105	1	0.2533152862260799
1105	2	0.36302121067387816
1105	3	0.34114091017207926
1105	4	0.3076586542056816
1105	5	0.2887379571050275
1105	6	0.2485866005343027
1105	7	0.2345139252475903
1105	8	0.33590795696967746
1105	9	-1.5633416802117068
1105	10	-0.08411106594839879
1105	11	0.0977328491988061
1105	12	0.29346052106473053
1105	13	0.3282510676730297
1105	14	-1.6737643782133205
1105	15	-0.23545018302793608
1105	16	-0.028164777237570604
1105	17	0.18772612060624994
1105	18	0.2833445285043865
1105	19	0.31734275556810587
1105	20	0.440503695092481
1105	21	0.5672113928776099
1105	22	0.6502721920188961
1105	23	0.8589961720651047
1105	24	0.6232922336206173
1105	25	0.3302441227077395
1106	1	0.11123133330235646
1106	2	0.11852357398909064
1106	3	0.23647598122035163
1106	4	-1.0450212387840452
1106	5	-0.320074936327714
1106	6	-0.09199511041661879
1106	7	0.025383252060485972
1106	8	0.15249603725256122
1106	9	0.3650225668185527
1106	10	0.4012065020291047
1106	11	0.296321842984014
1106	12	0.16592506561285347
1106	13	0.27446718766923084
1106	14	0.6579061061604108
1106	15	0.419883331174276
1106	16	0.23103841521263746
1106	17	0.09831565012965626
1106	18	0.11409429869787857
1106	19	0.15414362007556548
1106	20	0.38061823775178283
1106	21	0.5126722135873223
1106	22	0.5132912599489003
1106	23	0.7925412822520315
1106	24	0.37212488526142806
1106	25	0.4308961664688553
1107	1	0.4868388599698754
1107	2	0.3955339360023977
1107	3	0.38842750822858463
1107	4	0.6040119162697195
1107	5	0.50087009650494
1107	6	0.31279526569235827
1107	7	0.3881464983514462
1107	8	0.39082806772083245
1107	9	0.5717763995086997
1107	10	0.5375285482920376
1107	11	0.28063553076076175
1107	12	0.3794415236150286
1107	13	0.41945134034211407
1107	14	0.8997670462657084
1107	15	0.7168526701110736
1107	16	0.7040191002952696
1107	17	0.5288992741461556
1107	18	0.7629465638741948
1107	19	0.608506752611973
1107	20	0.7044273297807706
1107	21	0.8434102122308786
1107	22	0.6614702026269583
1107	23	0.9797639540275753
1107	24	0.957055430417725
1107	25	0.6694045989138075
1108	1	0.4122863919841464
1108	2	0.44579836150793284
1108	3	0.4180175716745289
1108	4	0.6993100870720568
1108	5	0.5815749087617164
1108	6	0.4170055665403751
1108	7	0.34902710834676043
1108	8	0.4699746136836396
1108	9	0.2072438484165815
1108	10	0.5641915753466193
1108	11	0.3774685174875927
1108	12	0.3757386027058587
1108	13	0.33424190573784074
1108	14	-0.8946804397944419
1108	15	0.0710024302804868
1108	16	0.43750496286354235
1108	17	0.5366613894675893
1108	18	0.6902628536798048
1108	19	0.5941513400171416
1108	20	0.6553908430712312
1108	21	0.7132896627022376
1108	22	0.7806755302755235
1108	23	1.0503112279931273
1108	24	0.9583776870300014
1108	25	0.7980720860995802
1109	1	0.3578735448790198
1109	2	0.30668100703674384
1109	3	0.43458312338056615
1109	4	0.49359184612504237
1109	5	0.5243347265624365
1109	6	0.320722677771073
1109	7	0.29241228999459423
1109	8	0.3770905602991448
1109	9	0.4921852793232375
1109	10	0.4037707518686702
1109	11	0.31331581094939054
1109	12	0.38398626519355616
1109	13	0.31606017058269487
1109	14	0.6604758420108254
1109	15	0.46231957654844535
1109	16	0.41872692319810056
1109	17	0.362191483662734
1109	18	0.5021454725017023
1109	19	0.4396200138444897
1109	20	0.7069999484961542
1109	21	0.6762513238889127
1109	22	0.6302205371688103
1109	23	1.1636049682106773
1109	24	0.8236902208893184
1109	25	0.6160393495112615
1110	1	0.30121678124707074
1110	2	0.13987909540393417
1110	3	0.3638160263179593
1110	4	0.5331454630132697
1110	5	0.39588125829171616
1110	6	0.2317148886344853
1110	7	0.25067818651104545
1110	8	0.33192925316986077
1110	9	0.41071681719937914
1110	10	0.4670688579844011
1110	11	0.2766902584325166
1110	12	0.19858135533855403
1110	13	0.31809727717632086
1110	14	0.6296911993218117
1110	15	0.47579589135814615
1110	16	0.351408829848431
1110	17	0.40732046773641173
1110	18	0.4910446109260249
1110	19	0.49429077764411744
1110	20	0.526185221028868
1110	21	0.6308801739744396
1110	22	0.7280674994631298
1110	23	0.8620538934296604
1110	24	0.749007543832839
1110	25	0.6213414274895541
1111	1	0.1755414323721496
1111	2	0.2315210546111146
1111	3	0.36040663987305877
1111	4	0.549660503394243
1111	5	0.39829485773538664
1111	6	0.3266149308436894
1111	7	0.3510514127644129
1111	8	0.3581159983625497
1111	9	0.359137154006298
1111	10	0.31489823269912876
1111	11	0.22855625989655692
1111	12	0.20521620767723903
1111	13	0.28538288342618756
1111	14	-1.7076763377756592
1111	15	-0.5076267962103671
1111	16	-0.018759037094540076
1111	17	0.17880650518444696
1111	18	0.27834315618302696
1111	19	0.27254280151904453
1111	20	0.2914208886221985
1111	21	0.4861044129324341
1111	22	0.25785550623231046
1111	23	-1.8084315043902208
1111	24	-0.2591782520023074
1111	25	-0.18976005158980058
1112	1	-0.16446905560244574
1112	2	-0.2582500388360742
1112	3	-0.06155500409624537
1112	4	0.11396497509620218
1112	5	-0.755079066023228
1112	6	-0.8050854110804622
1112	7	-0.9564827882128313
1112	8	-0.6849219145533425
1112	9	-0.6525930424721409
1112	10	-0.9398745802031243
1112	11	-0.7557003945478371
1112	12	-0.7574287085597078
1112	13	-0.771767477708309
1112	14	-0.6202726679500401
1112	15	-0.9294822361450841
1112	16	-0.7637029047069035
1112	17	-0.7710942204637519
1112	18	-0.979075820266452
1112	19	-0.7656087472675068
1112	20	-0.7833612340944143
1112	21	-1.7471427079058657
1112	22	-1.5749121503596868
1112	23	-1.2754190598933306
1112	24	-1.5466501977140368
1112	25	-1.8843194987599645
1113	1	-0.06233868642150853
1113	2	-0.21190497182407242
1113	3	-0.1997083472716703
1113	4	0.061989698996121945
1113	5	-0.09425460281507725
1113	6	-0.11309964690577345
1113	7	-0.073989170388856
1113	8	-0.03012902998789002
1113	9	0.1040220288949385
1113	10	-0.07655932189056358
1113	11	-0.29381622407712626
1113	12	-0.0940713482297113
1113	13	-0.24202913651940605
1113	14	-0.09837863473971709
1113	15	-0.23882206599265743
1113	16	-0.12840214422791973
1113	17	-0.1768322788644241
1113	18	-0.031898170371512435
1113	19	-0.2878861450311234
1113	20	-0.8614115273549032
1113	21	-0.8086295615606005
1113	22	-0.36793520589294815
1113	23	-0.41226416870726723
1113	24	-0.15772987536256267
1113	25	-1.4462600106873253
1114	1	-1.0341917074105993
1114	2	-0.8202052803652826
1114	3	-0.6074650005425255
1114	4	-1.707742493273038
1114	5	-0.9230183931588627
1114	6	-0.7087859564980477
1114	7	-0.6147141318647726
1114	8	-0.562880697105733
1114	9	-0.6646848554042393
1114	10	-0.6827445333673552
1114	11	-0.7675787964441199
1114	12	-0.7074066063149331
1114	13	-0.7341806412145218
1114	14	-0.7309422415930769
1114	15	-1.6897217229066044
1114	16	-1.0663853411827091
1114	17	-1.1398415103152573
1114	18	-0.9322742521730802
1114	19	-0.9984494505852908
1114	20	-2.182530722165744
1114	21	-6.882096701940853
1114	22	-7.785633086458418
1114	23	-8.025210410038621
1114	24	-6.117168633978924
1114	25	-7.324987765183479
1115	1	-1.147832460939675
1115	2	-1.0940559665454224
1115	3	-0.8135896192204832
1115	4	-0.6269756508510298
1115	5	-0.7470008559365624
1115	6	-0.9854685225579516
1115	7	-1.1450147676808697
1115	8	-0.8157708428342048
1115	9	-2.9024833481545294
1115	10	-1.7319831663388001
1115	11	-1.2977325703949774
1115	12	-1.1541755654633903
1115	13	-1.5014963734725697
1115	14	-1.168244197569635
1115	15	-2.0533062059927474
1115	16	-1.4033970055267204
1115	17	-3.2792597138434516
1115	18	-3.02521785881315
1115	19	-2.272653722669247
1115	20	-1.9699693235299591
1115	21	-1.4255678140523287
1115	22	-0.8077282146353042
1115	23	-0.3966140902054036
1115	24	-1.402537600470474
1115	25	-1.1592952556175007
1116	1	-1.5160759868569222
1116	2	-0.7417495829458964
1116	3	-0.7265436310010003
1116	4	-0.30789664349544665
1116	5	-0.3334880954955892
1116	6	-0.5208347516655536
1116	7	-0.7416788222947659
1116	8	-1.8237562421564633
1116	9	-0.838949187116917
1116	10	-2.4462870784107693
1116	11	-1.1065418390523831
1116	12	-0.9093652101491188
1116	13	-0.8589480183149192
1116	14	-1.0643232489063905
1116	15	-1.085337911057124
1116	16	-0.6965154532701092
1116	17	-0.6524798171026731
1116	18	-1.2166795839265063
1116	19	-0.947182158644992
1116	20	-1.2264590946883942
1116	21	-0.5494281920544424
1116	22	-0.19255519161598755
1116	23	-0.013233530743391952
1116	24	-0.09581918083213026
1116	25	0.04549182721836331
1201	1	0.18848896693778983
1201	2	0.33702905422777363
1201	3	0.26034435562426594
1201	4	0.35473907879516275
1201	5	0.3058906592581323
1201	6	0.2213571977750206
1201	7	0.2358693579701594
1201	8	0.3372287663864597
1201	9	0.7211457036622377
1201	10	0.5974839344311746
1201	11	0.4093470845389575
1201	12	0.34742457875793775
1201	13	0.32955669407324706
1201	14	1.0078823875875145
1201	15	0.7141733968372748
1201	16	0.7303717984798084
1201	17	0.6829085645180939
1201	18	0.6151703728995628
1201	19	0.5283028590838796
1201	20	0.7380496411422897
1201	21	0.8151838856218774
1201	22	0.8264413360980001
1201	23	0.7357215683729237
1201	24	0.7696531602970111
1201	25	0.7421812137811656
1202	1	-0.13224563672519096
1202	2	0.05744840465613521
1202	3	0.021580895155711488
1202	4	0.02377086430480091
1202	5	0.055806941421138845
1202	6	0.05032190297121275
1202	7	0.007473199333091429
1202	8	0.2682707571023215
1202	9	0.35371252928106856
1202	10	0.4831192337039809
1202	11	0.26598253265188276
1202	12	0.169440608801672
1202	13	0.33587009078293306
1202	14	0.5813487377890212
1202	15	0.5134204854600171
1202	16	0.4965069203185237
1202	17	0.34824142671372726
1202	18	0.2844884768202718
1202	19	0.34852519517700387
1202	20	0.6184171739978979
1202	21	0.5566647198991959
1202	22	0.49535404950653117
1202	23	0.8947661435290399
1202	24	0.4313390606680514
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2211	6	0.1267197877881685
2211	7	0.1085362708148736
2211	8	0.161206714884365
2211	9	0.29081904326692154
2211	10	0.31899621720311444
2211	11	0.12788274459870053
2211	12	0.18982771663414866
2211	13	0.0657901492578361
2211	14	0.35398160009280843
2211	15	0.3004423690507849
2211	16	0.1411853412982893
2211	17	0.33224508716372725
2211	18	0.19568445180856742
2211	19	0.2483275869127226
2211	20	0.1924627953020135
2211	21	0.3056591674201101
2211	22	0.3000720910256902
2211	23	0.5519042682447406
2211	24	0.5046644583567428
2211	25	0.5014077333461273
2212	1	-0.34165603828862956
2212	2	-0.3415896645609493
2212	3	-0.22336360001751032
2212	4	-0.5687358649518472
2212	5	-0.22054809767377037
2212	6	-0.663193508947451
2212	7	-0.6016596632054245
2212	8	-0.32594136741290214
2212	9	-0.2442169920676065
2212	10	-0.576045733896116
2212	11	-0.43923820304840433
2212	12	-0.6618520577232303
2212	13	-0.6622325685764068
2212	14	-0.3678014459960792
2212	15	-0.6531740341642731
2212	16	-0.44867696305969673
2212	17	-0.52416865082089
2212	18	-0.4557550689604142
2212	19	-0.3846289543873169
2212	20	-0.48524293704154076
2212	21	-0.4012127989930576
2212	22	-0.5096208948456891
2212	23	2.3812069454010043E-4
2212	24	-0.20582780464496153
2212	25	-0.9144710659374908
2213	1	-0.10252480591130464
2213	2	-1.296049959406048
2213	3	-0.2792669377661312
2213	4	0.07191648797364536
2213	5	-0.04271451467329257
2213	6	0.07021438568663996
2213	7	0.03249820227914313
2213	8	0.20026701844118833
2213	9	0.19225918974034073
2213	10	-0.31281114228164597
2213	11	0.002965355698052008
2213	12	0.10507102603541085
2213	13	-0.3206017071318499
2213	14	0.05098100725494703
2213	15	-0.009528570220957988
2213	16	0.023613820762307114
2213	17	0.21023029403359317
2213	18	0.15986622255342553
2213	19	0.23969865423944015
2213	20	0.20294751290057889
2213	21	-0.4460048827627361
2213	22	-0.9046962675759005
2213	23	-0.00738734142305475
2213	24	0.17060954299322617
2213	25	-0.3649111160665939
2214	1	-0.3004841026491185
2214	2	-0.4753650715008426
2214	3	-0.5006138815607777
2214	4	-0.604535840840434
2214	5	-0.309459276050184
2214	6	-0.26604459758584653
2214	7	-0.5724005063773916
2214	8	-0.19902125028665552
2214	9	-0.08102313495536606
2214	10	-2.0853667678403696
2214	11	-0.9666863150422174
2214	12	-0.5385606688711491
2214	13	-0.3351136451245793
2214	14	-0.9586059879176787
2214	15	-0.744113140623881
2214	16	-0.9217123407206813
2214	17	-0.6657382455818137
2214	18	-0.56225556512981
2214	19	-1.451369280810212
2214	20	-2.569386567525058
2214	21	-2.089101771498875
2214	22	-2.270983001312466
2214	23	-2.600275016973413
2214	24	-1.860662561782199
2214	25	-2.186191090176365
2215	1	-0.05231272214584948
2215	2	-0.2093892656139289
2215	3	-0.3981808323365783
2215	4	-0.03464780079150387
2215	5	0.09113766833750958
2215	6	-0.041073045755389614
2215	7	-0.05249898707554479
2215	8	-0.07837826982532903
2215	9	-0.14168819981338032
2215	10	-0.223995930286911
2215	11	-0.3975670472417221
2215	12	-0.2217090570761684
2215	13	-0.13086788142847183
2215	14	-0.003439929374636108
2215	15	-0.500666867628496
2215	16	-0.16744656088049226
2215	17	-0.043888690404209285
2215	18	-0.18528599171378346
2215	19	-1.839952695596125
2215	20	-2.2994341881072557
2215	21	-3.3197343627457983
2215	22	-2.4771423041512577
2215	23	-3.475954594505488
2215	24	-2.1410942758446154
2215	25	-1.5603047755282233
2216	1	-1.5993881634103921
2216	2	-1.2053077140169641
2216	3	-0.9950604801613316
2216	4	-1.012222604655605
2216	5	-1.9262020949767091
2216	6	-1.5875034402435304
2216	7	-1.2337594505133893
2216	8	-5.228193029060893
2216	9	-4.048095790086048
2216	10	-2.0395333169444
2216	11	-1.1136035044157993
2216	12	-1.249289554021658
2216	13	-2.0039815272915575
2216	14	-3.449798926606661
2216	15	-2.000754420044885
2216	16	-3.0299664368372774
2216	17	-2.684115626557407
2216	18	-2.730741060980577
2216	19	-1.690969793032039
2216	20	-1.0105174470488194
2216	21	-0.865403783230704
2216	22	-0.7252707081772485
2216	23	-1.210831635906402
2216	24	-0.7654662159039702
2216	25	-0.2758618324885376
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	60.0
8	253.0
9	786.0
10	1698.0
11	3366.0
12	5889.0
13	8839.0
14	11789.0
15	15174.0
16	17408.0
17	18158.0
18	19506.0
19	20031.0
20	20683.0
21	22010.0
22	23820.0
23	25347.0
24	27278.0
25	29453.0
26	31300.0
27	35528.0
28	40885.0
29	48148.0
30	58395.0
31	71608.0
32	94500.0
33	134487.0
34	192935.0
35	302506.0
36	517255.0
37	1638409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6818643052258	19.597006547195885	12.97650790805466	23.74462123952366
2	15.654965740969725	19.57908671680656	39.905558545846006	24.860388996377708
3	19.351678486997635	24.49707946899436	28.23905437352246	27.912187670485544
4	12.713785257473392	16.15620071809733	36.56805921684328	34.56195480758599
5	13.91058986736371	37.53173195290185	34.517411004328494	14.040267175405944
6	33.496066561100015	36.586291134118035	16.257855313568147	13.659786991213801
7	28.102475487308382	31.938338998779635	21.38588889325252	18.57329662065946
8	25.28505446252961	37.26657824972269	19.584168848241788	17.864198439505905
9	24.590967510709813	17.284702670718445	20.81427152624202	37.31005829232972
10	15.26974667367563	30.290679019043765	32.371404475865184	22.06816983141542
11	33.88902351644394	24.97120699631444	21.731874080536628	19.407895406704995
12	21.801295720399015	26.74341885165543	30.224351048881882	21.23093437906367
13	27.588612476075745	22.800212136401495	25.703123659056192	23.90805172846657
14	21.025996384558677	22.60479921616953	26.931139632020916	29.438064767250875
15	23.953147459594003	29.874094634265262	22.884585341984387	23.288172564156344
16	22.623063657267338	30.02913823439056	25.069878233832004	22.277919874510097
17	21.598678541484965	29.523094977794358	27.128475068758085	21.7497514119626
18	21.903349717695576	28.811109714716604	27.985607827256693	21.299932740331133
19	22.285126647641157	28.249245815987827	28.148823705668963	21.316803830702053
20	22.989019019406317	27.667084898308037	27.742546957968443	21.6013491243172
21	22.97036136875381	27.63914015885761	27.577564055160543	21.812934417228032
22	22.34804848474262	27.528323282079945	27.64794848168576	22.475679751491676
23	22.177216383884886	27.799332899157843	27.80314673394543	22.220303983011842
24	22.346697169800617	27.84369044477886	27.514319435187772	22.295292950232746
25	22.688189503899746	27.28955162440202	27.015389961729902	23.006868909968333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2928.0
1	2928.0
2	3275.0
3	3622.0
4	3622.0
5	3622.0
6	4802.5
7	5983.0
8	5983.0
9	5983.0
10	8823.5
11	11664.0
12	11664.0
13	11664.0
14	18633.0
15	25602.0
16	25602.0
17	25602.0
18	40136.5
19	54671.0
20	54671.0
21	54671.0
22	78641.5
23	102612.0
24	102612.0
25	102612.0
26	134731.5
27	166851.0
28	166851.0
29	166851.0
30	202503.5
31	238156.0
32	238156.0
33	238156.0
34	270547.0
35	302938.0
36	302938.0
37	302938.0
38	329200.5
39	355463.0
40	355463.0
41	355463.0
42	367666.0
43	379869.0
44	379869.0
45	379869.0
46	403733.5
47	427598.0
48	427598.0
49	427598.0
50	409925.5
51	392253.0
52	392253.0
53	392253.0
54	359642.0
55	327031.0
56	327031.0
57	327031.0
58	290813.0
59	254595.0
60	254595.0
61	254595.0
62	217766.0
63	180937.0
64	180937.0
65	180937.0
66	144792.5
67	108648.0
68	108648.0
69	108648.0
70	82446.5
71	56245.0
72	56245.0
73	56245.0
74	40979.5
75	25714.0
76	25714.0
77	25714.0
78	17637.5
79	9561.0
80	9561.0
81	9561.0
82	6411.0
83	3261.0
84	3261.0
85	3261.0
86	2122.0
87	983.0
88	983.0
89	983.0
90	622.5
91	262.0
92	262.0
93	262.0
94	154.5
95	47.0
96	47.0
97	47.0
98	29.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031185409184859368
2	0.013236344383499079
3	0.018327246069460262
4	0.018763609071114077
5	0.014894523789783579
6	0.005294537753399632
7	0.0
8	1.1636346710768421E-4
9	8.727260033076316E-5
10	5.8181733553842104E-5
11	0.001105452937523
12	0.0010181803371922368
13	0.0027345414770305787
14	7.854534029768683E-4
15	0.0023854510757075264
16	0.0028218140773613423
17	0.002647268876699816
18	0.002356360208930605
19	4.363630016538158E-4
20	4.6545386843073683E-4
21	0.07944715716777138
22	0.07592716228776394
23	0.07685807002462541
24	0.0012799981381845262
25	0.21562150455053886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3437505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.26643469768989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.98318891656336	56.7868320139751
2	11.796817736709125	16.342470108006246
3	3.1922687027423833	6.633512149079536
4	1.1998220437170544	3.3242958095990467
5	0.5782916504599191	2.0028100421400645
6	0.3251937869865126	1.3515008526237449
7	0.19606612149907976	0.9506560840871736
8	0.13378748861339965	0.741358587472638
9	0.10040786748326026	0.6259405496547192
>10	0.4574455258104722	5.5473339284849486
>50	0.02523513132807695	1.2022811058234355
>100	0.009027041685455317	1.1033388842185268
>500	0.00105516655738336	0.5401467858314968
>1k	0.0012239931965603804	1.7805899515189196
>5k	8.441332390071589E-5	0.3289235468515313
>10k+	8.441332390071589E-5	0.7380096006328377
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	13472	0.39191215721868045	No Hit
CCCATGTACTCTGCGTTGATACCAC	11772	0.3424576836979146	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6182	0.17983973841492595	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5069	0.1474616036922128	No Hit
GCGTTGATACCACTGCTTCCCATGT	4384	0.1275343599500219	No Hit
CATGTACTCTGCGTTGATACCACTG	4150	0.12072709712422236	No Hit
GTATCAACGCAGAGTACATGGGAAG	3621	0.10533802859923112	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3552	0.10333075879162357	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3540	0.10298166839030053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.8181733553842104E-5	0.0
10	0.0	0.0	0.0	8.727260033076316E-5	0.0
11	0.0	0.0	0.0	1.1636346710768421E-4	0.0
12	0.0	0.0	0.0	1.1636346710768421E-4	0.0
13	0.0	0.0	0.0	1.1636346710768421E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCT	55	1.9639048E-4	13.81597	4
GTCTAGG	260	0.0	10.232407	1
ACTATCG	280	0.0	10.174426	11
GTCTCGC	180	3.929017E-10	10.029383	1
TACACCG	125	2.5800473E-6	9.877701	5
CTATCGG	295	0.0	9.657083	12
GTCCTAG	410	0.0	9.501521	1
CTAGACA	360	0.0	9.49848	4
TATACCG	100	2.7634724E-4	9.497788	5
CGTGTTA	90	0.0011183954	9.496131	10
ACCGTTC	110	6.8717236E-5	9.496131	8
GTGTAGG	515	0.0	9.409273	1
GTCCTAA	325	0.0	9.355344	1
TATCGGT	340	0.0	9.216699	13
CCCGTAC	165	1.05897925E-7	9.216547	16
TAGTACG	95	0.0018272873	8.998559	4
CTGACGC	95	0.0018314036	8.996334	9
CGGTACC	85	0.007448354	8.939746	4
GTGTTAC	320	0.0	8.907676	1
CTAGGAC	300	0.0	8.865892	3
>>END_MODULE