FastQCFastQC Report
Wed 25 May 2016
SRR1294746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437505
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144570.4205666609939477No Hit
GTACATGGGAAGCAGTGGTATCAAC136650.3975266945066262No Hit
CCCATGTACTCTGCGTTGATACCAC131090.3813521725786581No Hit
GGTATCAACGCAGAGTACTTTTTTT109540.31866135467439316No Hit
TATCAACGCAGAGTACTTTTTTTTT98810.28744685462275693No Hit
GAGTACATGGGAAGCAGTGGTATCA69420.20194879716538594No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57350.16683612096564224No Hit
CATGTACTCTGCGTTGATACCACTG47710.13879252539269032No Hit
ACGCAGAGTACATGGGAAGCAGTGG46640.1356798026475598No Hit
GCGTTGATACCACTGCTTCCCATGT44680.12997799275928326No Hit
GTATCAACGCAGAGTACATGGGAAG40390.11749801091198414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC500.001477565213.3268491
TGTGCGA655.4880416E-513.145710
TAGACAG4500.011.2057555
GATCGGT600.00590527911.07646911
GTCTAGG3150.010.8790611
CGATGCG700.001499747910.85057615
ACCGTCC1053.4954446E-610.8504198
TACGCAG1002.361113E-510.4657545
ACCGTTC1002.4171612E-510.4435288
ACGCGTA1002.4171612E-510.4435289
CCTAGAC3700.010.2857533
TATCTCG750.00261606910.148615
TGATCGG951.6554541E-49.99380810
GCACCGT2002.5465852E-119.9699836
CGTAGAC1151.0090711E-59.9279873
CGGGACT1151.0090711E-59.9279874
ACCGTCT1151.0348902E-59.9069038
CTAGACT3600.09.7786084
GGCTTAG1606.439768E-89.5191781
TGCTACG900.00109869599.5145992
Produced by FastQC (version 0.11.5)