FastQCFastQC Report
Wed 25 May 2016
SRR1294745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530018
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1794643.9616619624910983No Hit
CCCATGTACTCTGCGTTGATACCAC1716713.7896317409776294No Hit
GAGTACATGGGAAGCAGTGGTATCA790601.745246928378651No Hit
CATGTACTCTGCGTTGATACCACTG616221.360303645592578No Hit
GCGTTGATACCACTGCTTCCCATGT547481.2085603191863696No Hit
GTATCAACGCAGAGTACATGGGAAG450740.9950070838570619No Hit
GCTTCCCATGTACTCTGCGTTGATA448490.9900402161757413No Hit
ACGCAGAGTACATGGGAAGCAGTGG443640.9793338569515617No Hit
ACTCTGCGTTGATACCACTGCTTCC361130.797193300335672No Hit
TATCAACGCAGAGTACATGGGAAGC353160.7795995512600612No Hit
GGTATCAACGCAGAGTACATGGGAA282820.624324230058247No Hit
CAGTGGTATCAACGCAGAGTACATG231170.5103070230626016No Hit
GTGGTATCAACGCAGAGTACATGGG215160.4749650001390723No Hit
GCAGTGGTATCAACGCAGAGTACAT187970.41494316358124844No Hit
ATACCACTGCTTCCCATGTACTCTG187080.41297849147619276No Hit
GTACTCTGCGTTGATACCACTGCTT173620.3832655852581601No Hit
GTACATGGTAAGCAGTGGTATCAAC169310.3737512742774974No Hit
CATGGGAAGCAGTGGTATCAACGCA164020.3620736164845261No Hit
ACATGGGAAGCAGTGGTATCAACGC162040.3577027729249641No Hit
GGGAAGCAGTGGTATCAACGCAGAG160030.3532657044629845No Hit
CTGCTTCCCATGTACTCTGCGTTGA159450.3519853563495774No Hit
TACCACTGCTTCCCATGTACTCTGC148290.3273496926502279No Hit
CTTCCCATGTACTCTGCGTTGATAC141010.3112791163302221No Hit
GATACCACTGCTTCCCATGTACTCT136230.3007272818783502No Hit
GCAGAGTACATGGGAAGCAGTGGTA129570.28602535354164155No Hit
AAGCAGTGGTATCAACGCAGAGTAC126510.2792704134950457No Hit
CCACTGCTTCCCATGTACTCTGCGT126130.2784315647310894No Hit
GTTGATACCACTGCTTCCCATGTAC125970.2780783652515288No Hit
CCATGTACTCTGCGTTGATACCACT125470.27697461687790204No Hit
ACCATGTACTCTGCGTTGATACCAC122270.2699106272866907No Hit
ATGGGAAGCAGTGGTATCAACGCAG104830.2314118840145889No Hit
TTCCCATGTACTCTGCGTTGATACC100230.22125739897722263No Hit
GTACATGGGTAAGCAGTGGTATCAA96650.2133545606220549No Hit
TGATACCACTGCTTCCCATGTACTC93480.20635679593326123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA90560.19991090543128084No Hit
CCCCATGTACTCTGCGTTGATACCA78230.17269247053764467No Hit
GGTAAGCAGTGGTATCAACGCAGAG76740.1694033003842369No Hit
ATCAACGCAGAGTACATGGGAAGCA75320.16626865500313687No Hit
GCTTACCATGTACTCTGCGTTGATA59230.13075003233982735No Hit
GGAAGCAGTGGTATCAACGCAGAGT53710.11856465029498779No Hit
GAGTACATGGTAAGCAGTGGTATCA52100.11501058053190959No Hit
AAAAAGTACTCTGCGTTGATACCAC51420.11350948274377719No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA50040.11046313723256729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA12200.013.5525831
TGACCGA500.001504478413.29511610
CGCAAAA3750.012.4126122
CGTAGAC803.7780165E-410.6861713
CGAACAT750.00264276310.13632615
CTACACT4800.010.0922724
ACACCGA951.650774E-49.9967696
GTCTGCG1151.0320242E-59.9094039
CTAGGAC2700.09.8506273
GTGCTAG2900.09.8300541
TATCGAC1553.947025E-89.82595419
GAACAAA18900.09.8040491
TAGGACA3200.09.7954414
ACGCAAA5650.09.5864771
GGTATCG1606.596383E-89.50459117
GTATCGA1502.6338785E-79.50448618
CACGGTA900.0011149739.4994482
TAACACG1106.85343E-59.4986094
TCTGTCG1701.6780177E-89.49640758
GTAAAAA8950.09.44931
Produced by FastQC (version 0.11.5)