##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294745_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4530018
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.919579568999506	33.0	33.0	33.0	27.0	33.0
2	30.952209240669685	33.0	33.0	33.0	27.0	33.0
3	30.933728960900375	33.0	33.0	33.0	27.0	33.0
4	34.2230986720141	37.0	37.0	37.0	27.0	37.0
5	34.70609631131708	37.0	37.0	37.0	27.0	37.0
6	34.77774260499627	37.0	37.0	37.0	27.0	37.0
7	34.81251531450869	37.0	37.0	37.0	33.0	37.0
8	34.698988613290275	37.0	37.0	37.0	27.0	37.0
9	34.38863178910106	37.0	37.0	37.0	27.0	37.0
10	34.52569945638185	37.0	37.0	37.0	27.0	37.0
11	34.782762673349204	37.0	37.0	37.0	27.0	37.0
12	34.72456798184908	37.0	37.0	37.0	27.0	37.0
13	34.5751729904826	37.0	37.0	37.0	27.0	37.0
14	35.823637124620696	40.0	37.0	40.0	27.0	40.0
15	36.036779324055665	40.0	37.0	40.0	27.0	40.0
16	36.01083240728845	40.0	37.0	40.0	27.0	40.0
17	36.020873426993006	40.0	37.0	40.0	27.0	40.0
18	36.00266003358044	40.0	37.0	40.0	27.0	40.0
19	35.97363167210373	40.0	37.0	40.0	27.0	40.0
20	35.924447761576225	40.0	37.0	40.0	27.0	40.0
21	35.84283572383156	40.0	37.0	40.0	27.0	40.0
22	35.77806335427365	40.0	37.0	40.0	27.0	40.0
23	35.44608078819996	37.0	37.0	40.0	27.0	40.0
24	35.78683815384398	37.0	37.0	40.0	27.0	40.0
25	34.68709572456445	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4173139635065084
1101	2	0.5414193245708887
1101	3	0.3864852499831599
1101	4	0.37552398209219007
1101	5	0.4119738389145198
1101	6	0.30888243639225976
1101	7	0.38534790447734224
1101	8	0.3630732707365212
1101	9	0.6393009492589812
1101	10	0.672957447498753
1101	11	0.45047124002425676
1101	12	0.48417744859710155
1101	13	0.5033266962037146
1101	14	1.1642380025594363
1101	15	0.8217846120907097
1101	16	0.7306621897323424
1101	17	0.8872581961985588
1101	18	0.8414954885581096
1101	19	0.8648289252063392
1101	20	0.9008147743626864
1101	21	0.8764550628210301
1101	22	0.9970255002799036
1101	23	1.2764579789585326
1101	24	1.01250787400312
1101	25	1.0053794280462611
1102	1	0.2902766265352952
1102	2	0.4760598255749855
1102	3	0.29137974813689027
1102	4	0.2632321590290587
1102	5	0.2695612192212664
1102	6	0.25832413123352893
1102	7	0.32098237122722395
1102	8	0.3315778584280409
1102	9	0.5582141476888651
1102	10	0.5956719397851771
1102	11	0.42724201979238075
1102	12	0.45244788313107875
1102	13	0.5349033510130639
1102	14	1.117611964579588
1102	15	0.8315013639225697
1102	16	0.6549465027907431
1102	17	0.7122264335411828
1102	18	0.7242374620035221
1102	19	0.7744076019686403
1102	20	0.9043332067430825
1102	21	0.921374636463355
1102	22	0.9092904779949222
1102	23	1.2191054210839454
1102	24	1.0411091384115778
1102	25	0.8851920237199948
1103	1	0.2629430757033404
1103	2	0.4198973980787102
1103	3	0.18605727221797608
1103	4	-0.07738813080062812
1103	5	0.20756594671437512
1103	6	0.13867247388764525
1103	7	0.21510285159628495
1103	8	0.3272579243418008
1103	9	0.5080634268004474
1103	10	0.519906262983568
1103	11	0.30513016017825123
1103	12	0.3354631156069061
1103	13	0.25271129699311956
1103	14	0.8796644810085397
1103	15	0.6583886320331374
1103	16	0.41891574863161196
1103	17	0.42392188365065664
1103	18	0.5719146424112438
1103	19	0.5163688656830772
1103	20	0.6950762911490571
1103	21	0.7121621283584787
1103	22	0.6015140309973006
1103	23	1.061469714123561
1103	24	0.6719147162659098
1103	25	0.6021470357175431
1104	1	0.3283986258107561
1104	2	0.5438307373943161
1104	3	0.343892875076218
1104	4	0.33423853742570486
1104	5	0.32569848397806567
1104	6	0.2745169465342343
1104	7	0.285946920973565
1104	8	0.37326340827031856
1104	9	0.5443129981598887
1104	10	0.6725827560681665
1104	11	0.4650548182539467
1104	12	0.3364290257296574
1104	13	0.38804173479937987
1104	14	0.8594759112098274
1104	15	0.6731619092185639
1104	16	0.6486676231662472
1104	17	0.6607960016659504
1104	18	0.5622842507539474
1104	19	0.6075824419180691
1104	20	0.7075580772635632
1104	21	0.7889837973272549
1104	22	0.857032945129248
1104	23	1.327704174901747
1104	24	0.9539409095424816
1104	25	0.7733368617936307
1105	1	0.15247926867736084
1105	2	0.27333020589015433
1105	3	0.16149440180925367
1105	4	0.08935277936024733
1105	5	0.19531575903435083
1105	6	0.16558249440299022
1105	7	0.22628844079931554
1105	8	0.2611014141561867
1105	9	-1.6574050877330038
1105	10	-0.20704166659177048
1105	11	0.032153613884041476
1105	12	0.053473473704649166
1105	13	0.12527442718168658
1105	14	-1.7122336305919816
1105	15	-0.32481545614973584
1105	16	-0.18374427315880837
1105	17	0.00863938988351265
1105	18	3.950392222833443E-4
1105	19	0.13353838474982638
1105	20	0.2468658555761678
1105	21	0.3448513996579692
1105	22	0.33882460178978846
1105	23	0.6413296668620703
1105	24	0.48622858577692085
1105	25	0.17914199359653082
1106	1	0.10642442024389709
1106	2	0.12766803391200554
1106	3	0.14383653354313353
1106	4	-1.0678292099819728
1106	5	-0.10810144136721789
1106	6	-0.004019601859020838
1106	7	0.11403240851621632
1106	8	0.21478494948726023
1106	9	0.4331181414496399
1106	10	0.3920875946054281
1106	11	0.29869901577702507
1106	12	0.13594116325557337
1106	13	0.20502640692320284
1106	14	0.6400882902244902
1106	15	0.39398085048843257
1106	16	0.29012535299676756
1106	17	0.14351479169867076
1106	18	0.3122529693739935
1106	19	0.2264088600511016
1106	20	0.405461282860216
1106	21	0.5255783021438845
1106	22	0.5371577902557476
1106	23	0.74128850845063
1106	24	0.4513112802049264
1106	25	0.4540361189265738
1107	1	0.21518972064708564
1107	2	0.2238115186974845
1107	3	0.19158825568600335
1107	4	0.2850934608310993
1107	5	0.1783148742414582
1107	6	0.05196043755589841
1107	7	0.009962546468443634
1107	8	0.11215789059991721
1107	9	0.3274608343205969
1107	10	0.2894026789497488
1107	11	0.14435885413967497
1107	12	0.0958428067494026
1107	13	0.08058192230044625
1107	14	0.6642796514857707
1107	15	0.2793250078588372
1107	16	0.3166454491836461
1107	17	0.3670046396438522
1107	18	0.42897357542364034
1107	19	0.32059787088116565
1107	20	0.3773700138081608
1107	21	0.4122278528499166
1107	22	0.38230595782341226
1107	23	0.6736521382396319
1107	24	0.5708433635909955
1107	25	0.4786704460704385
1108	1	0.4867502639411363
1108	2	0.4543814337379253
1108	3	0.5409460013568115
1108	4	0.6730436562149009
1108	5	0.5832484679903303
1108	6	0.4019788258841075
1108	7	0.41691563367298556
1108	8	0.46083791839356536
1108	9	0.29276052462902413
1108	10	0.48184035752407794
1108	11	0.3192372905998013
1108	12	0.3619406745979745
1108	13	0.37937869168810323
1108	14	-1.0447416729144479
1108	15	0.044319143122095284
1108	16	0.4247532829962566
1108	17	0.4833709636164727
1108	18	0.6605541991761257
1108	19	0.5982860131078382
1108	20	0.6881349249722817
1108	21	0.784324307928479
1108	22	0.822714595248776
1108	23	1.0308853119573413
1108	24	0.9783256383104018
1108	25	0.8245807770209055
1109	1	0.4727485837395129
1109	2	0.3604301595850252
1109	3	0.4895598350168484
1109	4	0.5926740118575751
1109	5	0.6080314419472046
1109	6	0.35479316360463287
1109	7	0.3670696521552088
1109	8	0.42723388590966493
1109	9	0.5300890040465518
1109	10	0.42723380473774597
1109	11	0.38756548998372864
1109	12	0.4046457992129291
1109	13	0.3834391789465741
1109	14	0.7231104693426218
1109	15	0.47669102774824523
1109	16	0.3879288640659837
1109	17	0.4695093002168633
1109	18	0.46075022269894816
1109	19	0.3910611461238034
1109	20	0.5432175348854713
1109	21	0.5432153748565085
1109	22	0.7602671015495304
1109	23	1.0642093108372066
1109	24	0.817306315991722
1109	25	0.7297894020559141
1110	1	0.3248161845086095
1110	2	0.24907463267058816
1110	3	0.3740802383348125
1110	4	0.5653346345139383
1110	5	0.4021451897032122
1110	6	0.1949642836559491
1110	7	0.2835268733985643
1110	8	0.30345242343851453
1110	9	0.4402318637017899
1110	10	0.4222110764064766
1110	11	0.25636118508066374
1110	12	0.18370979897019168
1110	13	0.157136525043569
1110	14	0.5820975284660435
1110	15	0.3349998605569766
1110	16	0.34235953101171646
1110	17	0.3047511137755521
1110	18	0.37100927020977537
1110	19	0.4405679565062641
1110	20	0.45793856710982794
1110	21	0.5878893154395257
1110	22	0.6266319348947462
1110	23	0.772798186912965
1110	24	0.6478826228297052
1110	25	0.6659946742248053
1111	1	0.15132381481502222
1111	2	0.09752541784195401
1111	3	0.23804733815886436
1111	4	0.4954520981289505
1111	5	0.36028009267266015
1111	6	0.20000775249523173
1111	7	0.2003485015898363
1111	8	0.24739917263827493
1111	9	0.23078983353858007
1111	10	0.2148976650153145
1111	11	0.16708733021547317
1111	12	0.14142610090373609
1111	13	0.0991156703644549
1111	14	-1.800464059259511
1111	15	-0.5650427721166125
1111	16	-0.12506284101371534
1111	17	0.09383592453123413
1111	18	0.23724951592471655
1111	19	0.24899396290823006
1111	20	0.31835542515243986
1111	21	0.4364580588591309
1111	22	0.1501432655287971
1111	23	-1.7543546806642993
1111	24	-0.4307931733531376
1111	25	-0.07950352743009859
1112	1	-0.17899489535301072
1112	2	-0.256996135771427
1112	3	-0.029281825078346202
1112	4	0.15105416823411844
1112	5	-0.626630947797473
1112	6	-0.8808494554399857
1112	7	-0.93871122128904
1112	8	-0.7727913401964344
1112	9	-0.6043060172671986
1112	10	-0.8574470839032173
1112	11	-0.8164400701836243
1112	12	-0.8503613929822293
1112	13	-0.8147804595770225
1112	14	-0.6733714716915813
1112	15	-0.9572296042803714
1112	16	-0.8387653001483741
1112	17	-0.9772652911972841
1112	18	-1.0334142299202327
1112	19	-0.9157409080913297
1112	20	-0.9911186726702823
1112	21	-1.844565160403043
1112	22	-1.7750106175518212
1112	23	-1.488783418936471
1112	24	-1.7905848016416712
1112	25	-2.071516108500596
1113	1	0.04478346609048245
1113	2	-0.16197499336190546
1113	3	0.030997806639987857
1113	4	0.23797773180843507
1113	5	0.09041344833737952
1113	6	-0.03064308741197408
1113	7	0.048256304541489214
1113	8	0.0952848748047046
1113	9	0.28002155362685954
1113	10	-0.06042488766178877
1113	11	-0.140022450519794
1113	12	-0.024729550338953743
1113	13	-0.1907110232515734
1113	14	0.018184069933596447
1113	15	-0.1181277568267447
1113	16	-0.0467368389574645
1113	17	0.06757573147704932
1113	18	0.023371733813156936
1113	19	-0.17671600433654788
1113	20	-0.6208976356652229
1113	21	-0.4698807612884437
1113	22	-0.09079871217289082
1113	23	-0.17636214741640543
1113	24	0.07588260184692075
1113	25	-0.9657840280838812
1114	1	-0.8346694957472209
1114	2	-0.6015418775067332
1114	3	-0.4170278293352645
1114	4	-1.4908827877514312
1114	5	-0.7787514250951162
1114	6	-0.48657128124965254
1114	7	-0.5493705087972458
1114	8	-0.46382501063561676
1114	9	-0.4108830916603239
1114	10	-0.4733539526764474
1114	11	-0.5125498649981921
1114	12	-0.4372729655474643
1114	13	-0.5668487774605779
1114	14	-0.5045322773357199
1114	15	-1.2373675470586534
1114	16	-0.7245943558628909
1114	17	-0.7608908066829585
1114	18	-0.6665471553413695
1114	19	-0.6395522630464114
1114	20	-1.7908230420242788
1114	21	-6.534159243606457
1114	22	-7.564789429244165
1114	23	-7.693818923962628
1114	24	-5.717330628232663
1114	25	-7.049430369281787
1115	1	-1.0662841131781597
1115	2	-1.0570095812618412
1115	3	-0.7965309929046995
1115	4	-0.6503127155805757
1115	5	-0.689661866523231
1115	6	-0.9399201446019916
1115	7	-0.9859375706498099
1115	8	-0.7570297416974938
1115	9	-2.7979707800197957
1115	10	-1.555815141103274
1115	11	-1.1972157828486019
1115	12	-1.0355361346927765
1115	13	-1.481365767110944
1115	14	-1.1435719678503276
1115	15	-2.048576812188969
1115	16	-1.4649483485519923
1115	17	-3.1706386870055994
1115	18	-2.866920478402399
1115	19	-2.1245460272242767
1115	20	-1.8578147687419104
1115	21	-1.26180690729759
1115	22	-0.7952768512180342
1115	23	-0.34028459930369337
1115	24	-1.2360743506519967
1115	25	-1.0336546367837016
1116	1	-1.5002637054203092
1116	2	-0.8371903767605886
1116	3	-0.5951663401295058
1116	4	-0.3296159904944034
1116	5	-0.397747170098647
1116	6	-0.42210400801860004
1116	7	-0.8178580388341601
1116	8	-1.7996788566281126
1116	9	-0.9340188329899561
1116	10	-2.5178034075996507
1116	11	-1.219594029598916
1116	12	-0.7333549476678911
1116	13	-0.8318572877168151
1116	14	-1.2153773006240627
1116	15	-1.227563633578292
1116	16	-0.8882395663391307
1116	17	-0.6914103944026664
1116	18	-1.352185442989871
1116	19	-0.9019601885511008
1116	20	-1.0075077783028732
1116	21	-0.5989493913864194
1116	22	-0.1867434416502789
1116	23	-0.12202951280495
1116	24	-0.2876161292714201
1116	25	-0.14710336076608854
1201	1	0.27223952189209655
1201	2	0.4321823752620624
1201	3	0.314465748791104
1201	4	0.38002999024301687
1201	5	0.40707523721935246
1201	6	0.2823363946169479
1201	7	0.2993262501091465
1201	8	0.44150338362710784
1201	9	0.6547714442795538
1201	10	0.5603439837655841
1201	11	0.3859744189822436
1201	12	0.4493711966603797
1201	13	0.44190974002555095
1201	14	1.0723308041153672
1201	15	0.7924914652204151
1201	16	0.6605930596776517
1201	17	0.7241750478250708
1201	18	0.5541151246175957
1201	19	0.6303691213267584
1201	20	0.6920839138228274
1201	21	0.7670917277829545
1201	22	0.8164330997152405
1201	23	0.5975111121909293
1201	24	0.6874819880223626
1201	25	0.5213216651492516
1202	1	-0.25661402451924076
1202	2	0.06522253127979738
1202	3	-0.05137770460654778
1202	4	-0.07801875941056835
1202	5	-0.05443284190245379
1202	6	-0.10640896031125635
1202	7	-0.13521676628280233
1202	8	-0.04920180332219104
1202	9	0.16416449988571458
1202	10	0.25206231805764645
1202	11	0.039396972473490166
1202	12	-0.08080898078440413
1202	13	0.06085504512170559
1202	14	0.2810721281315054
1202	15	0.342158655049964
1202	16	0.2621281323849587
1202	17	0.0018787353965379339
1202	18	0.04899496461035113
1202	19	0.10384172216581078
1202	20	0.3024176643247358
1202	21	0.2563934562262702
1202	22	0.2168124802153102
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2211	3	0.3027561101081133
2211	4	0.4812323339089417
2211	5	0.388968890585943
2211	6	0.25953805632239124
2211	7	0.28138595567529734
2211	8	0.35116248105019565
2211	9	0.49911784765917844
2211	10	0.4177025555539373
2211	11	0.2660235245748055
2211	12	0.2749500032221377
2211	13	0.1619006127019773
2211	14	0.5418800112253592
2211	15	0.36002811227516673
2211	16	0.26042870868101886
2211	17	0.3599621075139936
2211	18	0.3172381485871725
2211	19	0.3661597313522478
2211	20	0.4207708921128912
2211	21	0.4232218461386026
2211	22	0.5320831312059866
2211	23	0.7029137628870004
2211	24	0.6904161932591819
2211	25	0.6887229926052925
2212	1	-0.25680086251464473
2212	2	-0.3248623873830425
2212	3	-0.16923093074650808
2212	4	-0.4443022993889585
2212	5	-0.22212527974485852
2212	6	-0.44527239249876516
2212	7	-0.5014267795996403
2212	8	-0.27092641329126366
2212	9	-0.20122401728750106
2212	10	-0.5206088142547074
2212	11	-0.4016365415698786
2212	12	-0.60790004904608
2212	13	-0.5268822426610313
2212	14	-0.4626362038197982
2212	15	-0.5866991614119641
2212	16	-0.34577844050161843
2212	17	-0.41587827535079214
2212	18	-0.3705827462342981
2212	19	-0.4648676860005807
2212	20	-0.4194841682754742
2212	21	-0.48654123586683085
2212	22	-0.4691272284515051
2212	23	-0.015216985109915981
2212	24	-0.18721286810576032
2212	25	-0.9877021938752719
2213	1	-0.10805915271455291
2213	2	-1.1811029192460936
2213	3	-0.2229835674246452
2213	4	0.12415890203385516
2213	5	-0.05301510893534811
2213	6	0.04152303891035558
2213	7	0.04601510979984624
2213	8	0.1722459487537833
2213	9	0.16448056186352744
2213	10	-0.4061846824240618
2213	11	-0.11715032756128352
2213	12	-0.012726614857527352
2213	13	-0.3564025928433736
2213	14	0.07330149358305249
2213	15	-0.07964974104914546
2213	16	0.007602017993335153
2213	17	0.14622172046355075
2213	18	0.023170635414615504
2213	19	0.011300122890602893
2213	20	0.051187829924302264
2213	21	-0.44472879109162733
2213	22	-0.8341246997749323
2213	23	-0.09346694891738849
2213	24	0.015358625088317979
2213	25	-0.564929474090647
2214	1	-0.19436110158247288
2214	2	-0.3959627515474935
2214	3	-0.3247782166055728
2214	4	-0.3717506838983482
2214	5	-0.25869607034638875
2214	6	-0.17104788267036497
2214	7	-0.43599384631064453
2214	8	-0.04813643756225616
2214	9	-0.003369971619072487
2214	10	-1.8522050773763468
2214	11	-0.8294288047178711
2214	12	-0.4945912526910021
2214	13	-0.2808906247076308
2214	14	-0.7843218623690262
2214	15	-0.46412505462724596
2214	16	-0.7006020381499027
2214	17	-0.46595965600533873
2214	18	-0.43361993934529863
2214	19	-1.3351604618459305
2214	20	-2.254283698630914
2214	21	-1.6782445214030375
2214	22	-1.9718763047921826
2214	23	-2.526933938491439
2214	24	-1.469709954652295
2214	25	-1.759278214066434
2215	1	-0.24692806332580375
2215	2	-0.35401251622832675
2215	3	-0.8434087643502224
2215	4	-0.3291111668518596
2215	5	-0.20500542458388082
2215	6	-0.26618129696235826
2215	7	-0.2658389904800842
2215	8	-0.30788758594786003
2215	9	-0.19049144762348647
2215	10	-0.2721580890053801
2215	11	-0.6154845123069776
2215	12	-0.4995340377587354
2215	13	-0.3340065575427431
2215	14	-0.1960837690082542
2215	15	-0.7167940774594115
2215	16	-0.5854236327712812
2215	17	-0.3203201431198579
2215	18	-0.3734357392326402
2215	19	-2.1765095794616443
2215	20	-2.4321600472650786
2215	21	-3.222857890668678
2215	22	-2.3867682423293672
2215	23	-3.463078406045547
2215	24	-2.41761838819248
2215	25	-1.798271047468866
2216	1	-1.366142267605312
2216	2	-0.9469445227572351
2216	3	-0.6017776798821579
2216	4	-0.7200533470213202
2216	5	-1.6761257523451434
2216	6	-1.3680192301242613
2216	7	-1.006573923379122
2216	8	-4.891376574022885
2216	9	-3.811697099834163
2216	10	-1.7531168188928277
2216	11	-0.845970484079217
2216	12	-0.9431840077255771
2216	13	-1.757693490734809
2216	14	-3.14798881263804
2216	15	-1.7917368264704976
2216	16	-2.747788362926144
2216	17	-2.44569669664925
2216	18	-2.453794053488288
2216	19	-1.4750315527092255
2216	20	-0.7798434831717884
2216	21	-0.5801547356246459
2216	22	-0.5106997314979722
2216	23	-0.7940849274545485
2216	24	-0.3298642180355742
2216	25	-0.13207655594084855
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	133.0
8	592.0
9	1572.0
10	2647.0
11	4685.0
12	7451.0
13	10915.0
14	14776.0
15	21583.0
16	26277.0
17	28998.0
18	29449.0
19	29914.0
20	30570.0
21	32487.0
22	33920.0
23	34686.0
24	37009.0
25	39079.0
26	41132.0
27	44946.0
28	50486.0
29	58404.0
30	70775.0
31	86085.0
32	115451.0
33	163463.0
34	238615.0
35	381015.0
36	671468.0
37	2221428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.063133437646705	19.187521612343776	11.467691781811052	24.281653168198467
2	12.354165732946141	22.473871096008697	37.545654794010034	27.626308377035137
3	20.977769868471224	29.052379456243514	26.772401632722275	23.197449042562987
4	12.946622063063124	19.584679540948816	33.46111495908331	34.007583436904746
5	11.520754726146157	40.425774318282635	35.00773771818014	13.045733237391074
6	32.332054257652985	33.524859848820185	18.403544881603633	15.7395410119232
7	24.628506161431222	33.554557066142394	22.8187306096265	18.99820616279989
8	26.887013518253738	36.19349798419364	21.23845102891887	15.681037468633752
9	26.800872502763273	17.94518137810138	20.31869176683134	34.93525435230401
10	16.0120883269058	32.34552394155396	32.046604959392845	19.5957827721474
11	28.954083941542997	28.036305823315956	19.556036010898104	23.45357422424294
12	23.36421282032942	31.188877909908193	28.65803153645978	16.788877733302606
13	28.386221445066557	22.56921717411983	23.031411889289608	26.013149491524
14	21.051001504641075	26.83775483533717	25.83967183990054	26.27157182012122
15	30.84318008124643	25.438848615936433	21.39959584293063	22.318375459886504
16	18.922949190789126	29.41206077740669	31.238146831712	20.426843200092186
17	25.269905786290863	29.991871810369908	27.329017725281318	17.409204678057904
18	25.644392424953523	26.465686194432315	28.485542589437447	19.404378791176722
19	22.661638556653102	31.40287668937675	29.196364061007518	16.739120692962626
20	23.301762360085696	28.176017465764385	27.423391364910195	21.098828809239727
21	19.246873572218252	30.236871477770126	30.196838355442868	20.31941659456875
22	21.394179893010985	29.418621143387107	22.876777940132282	26.310421023469626
23	17.900495558180644	30.834274660431856	27.409066476729578	23.85616330465793
24	20.786989238125702	35.238929680154826	24.430057309392353	19.544023772327122
25	21.866287653432988	26.93606165042003	22.46432203852952	28.733328657617463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12196.0
1	12196.0
2	14797.0
3	17398.0
4	17398.0
5	17398.0
6	21185.0
7	24972.0
8	24972.0
9	24972.0
10	26087.5
11	27203.0
12	27203.0
13	27203.0
14	32919.0
15	38635.0
16	38635.0
17	38635.0
18	52717.5
19	66800.0
20	66800.0
21	66800.0
22	90641.0
23	114482.0
24	114482.0
25	114482.0
26	145624.5
27	176767.0
28	176767.0
29	176767.0
30	210288.5
31	243810.0
32	243810.0
33	243810.0
34	272764.0
35	301718.0
36	301718.0
37	301718.0
38	327729.5
39	353741.0
40	353741.0
41	353741.0
42	405773.5
43	457806.0
44	457806.0
45	457806.0
46	781827.0
47	1105848.0
48	1105848.0
49	1105848.0
50	950416.0
51	794984.0
52	794984.0
53	794984.0
54	576436.0
55	357888.0
56	357888.0
57	357888.0
58	275506.0
59	193124.0
60	193124.0
61	193124.0
62	158117.0
63	123110.0
64	123110.0
65	123110.0
66	94979.0
67	66848.0
68	66848.0
69	66848.0
70	49430.5
71	32013.0
72	32013.0
73	32013.0
74	22718.5
75	13424.0
76	13424.0
77	13424.0
78	9176.5
79	4929.0
80	4929.0
81	4929.0
82	3295.5
83	1662.0
84	1662.0
85	1662.0
86	1070.5
87	479.0
88	479.0
89	479.0
90	305.5
91	132.0
92	132.0
93	132.0
94	83.0
95	34.0
96	34.0
97	34.0
98	24.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02993365589275804
2	0.014282503954730422
3	0.01975709588791921
4	0.020684244521765698
5	0.019977845562644564
6	0.009823360525278265
7	0.0014127979182422675
8	0.0015231727556049447
9	0.001346573015824661
10	6.18099089230992E-4
11	0.001236198178461984
12	0.003311245120880315
13	0.0024061714545063617
14	7.505488940662046E-4
15	0.002119196877363401
16	0.0024503213894514326
17	0.0022957966171436844
18	0.0020308970074732597
19	4.194243819781731E-4
20	2.8697457714296055E-4
21	0.08313432750156843
22	0.07885178381189654
23	0.07962440767343529
24	9.933735362640942E-4
25	0.22540749286205927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4530018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.01704426222656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34181843583113	47.719639166538734
2	8.419491190630126	9.095920566193882
3	1.6724943178007625	2.710295987788987
4	0.5466611203162945	1.1811607173025458
5	0.27000052414044917	0.7292315131659508
6	0.1514529703201429	0.4908625080857308
7	0.10557120215191158	0.39918510096193943
8	0.07261891937326453	0.31381275056485547
9	0.05314975101731441	0.2583893207905735
>10	0.3136291126060356	3.122269263792725
>50	0.026146595430569148	0.9808457800052612
>100	0.019417200157658514	2.151196578615958
>500	0.003027759328240662	1.1832550531215296
>1k	0.0027374262307566775	3.2729837485635986
>5k	4.562377051261129E-4	1.741881198204662
>10k+	0.001327236960366874	24.649070746303067
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	179464	3.9616619624910983	No Hit
CCCATGTACTCTGCGTTGATACCAC	171671	3.7896317409776294	No Hit
GAGTACATGGGAAGCAGTGGTATCA	79060	1.745246928378651	No Hit
CATGTACTCTGCGTTGATACCACTG	61622	1.360303645592578	No Hit
GCGTTGATACCACTGCTTCCCATGT	54748	1.2085603191863696	No Hit
GTATCAACGCAGAGTACATGGGAAG	45074	0.9950070838570619	No Hit
GCTTCCCATGTACTCTGCGTTGATA	44849	0.9900402161757413	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	44364	0.9793338569515617	No Hit
ACTCTGCGTTGATACCACTGCTTCC	36113	0.797193300335672	No Hit
TATCAACGCAGAGTACATGGGAAGC	35316	0.7795995512600612	No Hit
GGTATCAACGCAGAGTACATGGGAA	28282	0.624324230058247	No Hit
CAGTGGTATCAACGCAGAGTACATG	23117	0.5103070230626016	No Hit
GTGGTATCAACGCAGAGTACATGGG	21516	0.4749650001390723	No Hit
GCAGTGGTATCAACGCAGAGTACAT	18797	0.41494316358124844	No Hit
ATACCACTGCTTCCCATGTACTCTG	18708	0.41297849147619276	No Hit
GTACTCTGCGTTGATACCACTGCTT	17362	0.3832655852581601	No Hit
GTACATGGTAAGCAGTGGTATCAAC	16931	0.3737512742774974	No Hit
CATGGGAAGCAGTGGTATCAACGCA	16402	0.3620736164845261	No Hit
ACATGGGAAGCAGTGGTATCAACGC	16204	0.3577027729249641	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	16003	0.3532657044629845	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	15945	0.3519853563495774	No Hit
TACCACTGCTTCCCATGTACTCTGC	14829	0.3273496926502279	No Hit
CTTCCCATGTACTCTGCGTTGATAC	14101	0.3112791163302221	No Hit
GATACCACTGCTTCCCATGTACTCT	13623	0.3007272818783502	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	12957	0.28602535354164155	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	12651	0.2792704134950457	No Hit
CCACTGCTTCCCATGTACTCTGCGT	12613	0.2784315647310894	No Hit
GTTGATACCACTGCTTCCCATGTAC	12597	0.2780783652515288	No Hit
CCATGTACTCTGCGTTGATACCACT	12547	0.27697461687790204	No Hit
ACCATGTACTCTGCGTTGATACCAC	12227	0.2699106272866907	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10483	0.2314118840145889	No Hit
TTCCCATGTACTCTGCGTTGATACC	10023	0.22125739897722263	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9665	0.2133545606220549	No Hit
TGATACCACTGCTTCCCATGTACTC	9348	0.20635679593326123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9056	0.19991090543128084	No Hit
CCCCATGTACTCTGCGTTGATACCA	7823	0.17269247053764467	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7674	0.1694033003842369	No Hit
ATCAACGCAGAGTACATGGGAAGCA	7532	0.16626865500313687	No Hit
GCTTACCATGTACTCTGCGTTGATA	5923	0.13075003233982735	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	5371	0.11856465029498779	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5210	0.11501058053190959	No Hit
AAAAAGTACTCTGCGTTGATACCAC	5142	0.11350948274377719	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5004	0.11046313723256729	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.207496747253543E-5	0.0
2	0.0	0.0	0.0	2.207496747253543E-5	0.0
3	0.0	0.0	0.0	2.207496747253543E-5	0.0
4	0.0	0.0	0.0	2.207496747253543E-5	0.0
5	0.0	0.0	0.0	2.207496747253543E-5	0.0
6	0.0	0.0	0.0	2.207496747253543E-5	0.0
7	0.0	0.0	0.0	2.207496747253543E-5	0.0
8	0.0	0.0	0.0	2.207496747253543E-5	0.0
9	0.0	0.0	0.0	6.62249024176063E-5	0.0
10	0.0	0.0	0.0	8.829986989014172E-5	0.0
11	0.0	0.0	0.0	1.7659973978028344E-4	0.0
12	0.0	0.0	0.0	3.531994795605669E-4	0.0
13	0.0	0.0	0.0	3.9734941450563773E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	1220	0.0	13.552583	1
TGACCGA	50	0.0015044784	13.295116	10
CGCAAAA	375	0.0	12.412612	2
CGTAGAC	80	3.7780165E-4	10.686171	3
CGAACAT	75	0.002642763	10.136326	15
CTACACT	480	0.0	10.092272	4
ACACCGA	95	1.650774E-4	9.996769	6
GTCTGCG	115	1.0320242E-5	9.909403	9
CTAGGAC	270	0.0	9.850627	3
GTGCTAG	290	0.0	9.830054	1
TATCGAC	155	3.947025E-8	9.825954	19
GAACAAA	1890	0.0	9.804049	1
TAGGACA	320	0.0	9.795441	4
ACGCAAA	565	0.0	9.586477	1
GGTATCG	160	6.596383E-8	9.504591	17
GTATCGA	150	2.6338785E-7	9.504486	18
CACGGTA	90	0.001114973	9.499448	2
TAACACG	110	6.85343E-5	9.498609	4
TCTGTCG	170	1.6780177E-8	9.4964075	8
GTAAAAA	895	0.0	9.4493	1
>>END_MODULE