FastQCFastQC Report
Wed 25 May 2016
SRR1294745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530018
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1891244.174906148275791No Hit
GTACATGGGAAGCAGTGGTATCAAC1702413.7580645374919044No Hit
GAGTACATGGGAAGCAGTGGTATCA841401.857387763139131No Hit
CATGTACTCTGCGTTGATACCACTG708841.5647619943232014No Hit
GCGTTGATACCACTGCTTCCCATGT597671.319354580931025No Hit
ACGCAGAGTACATGGGAAGCAGTGG538911.1896420720624068No Hit
GCTTCCCATGTACTCTGCGTTGATA509941.1256908912944719No Hit
GTATCAACGCAGAGTACATGGGAAG471221.0402166172408145No Hit
ACTCTGCGTTGATACCACTGCTTCC422900.9335503744135233No Hit
TATCAACGCAGAGTACATGGGAAGC406590.897546102465818No Hit
GGTATCAACGCAGAGTACATGGGAA309650.6835513677870595No Hit
CAGTGGTATCAACGCAGAGTACATG278150.614015220248573No Hit
GTGGTATCAACGCAGAGTACATGGG227810.5028898339918296No Hit
CATGGGAAGCAGTGGTATCAACGCA214250.4729561780990716No Hit
ATACCACTGCTTCCCATGTACTCTG214050.47251467874962083No Hit
GCAGTGGTATCAACGCAGAGTACAT213080.4703734069047849No Hit
ACATGGGAAGCAGTGGTATCAACGC204380.4511681852036791No Hit
GGGAAGCAGTGGTATCAACGCAGAG187610.41414846475223716No Hit
CTGCTTCCCATGTACTCTGCGTTGA179330.39587039168497784No Hit
GTACTCTGCGTTGATACCACTGCTT171830.3793141660805763No Hit
CTTCCCATGTACTCTGCGTTGATAC168510.3719852768796945No Hit
TACCACTGCTTCCCATGTACTCTGC164000.362029466549581No Hit
GATACCACTGCTTCCCATGTACTCT158180.3491818354805654No Hit
AAGCAGTGGTATCAACGCAGAGTAC153850.33962337456495756No Hit
GTACATGGTAAGCAGTGGTATCAAC153540.338939050573309No Hit
CCATGTACTCTGCGTTGATACCACT151610.3346785818511096No Hit
GCAGAGTACATGGGAAGCAGTGGTA150170.3314997865350645No Hit
CCACTGCTTCCCATGTACTCTGCGT143560.31690823303571863No Hit
ACCATGTACTCTGCGTTGATACCAC136110.3004623822686797No Hit
GTTGATACCACTGCTTCCCATGTAC135650.2994469337649431No Hit
ATGGGAAGCAGTGGTATCAACGCAG128470.2835971071196627No Hit
GTATCAACGCAGAGTACTTTTTTTT110450.24381801573415382No Hit
TTCCCATGTACTCTGCGTTGATACC105630.2331778814123917No Hit
GTACATGGGTAAGCAGTGGTATCAA99400.21942517667700215No Hit
TGATACCACTGCTTCCCATGTACTC97100.21434793415831904No Hit
CCCCATGTACTCTGCGTTGATACCA89980.19863055731787377No Hit
GGTAAGCAGTGGTATCAACGCAGAG89160.1968204099851259No Hit
ATCAACGCAGAGTACATGGGAAGCA87870.1939727391811688No Hit
TATCAACGCAGAGTACTTTTTTTTT87280.19267031610028923No Hit
GGTATCAACGCAGAGTACTTTTTTT75620.16693090402731292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA75020.1656064059789608No Hit
GCTTACCATGTACTCTGCGTTGATA67720.14949167972400992No Hit
GTACTTTTTTTTTTTTTTTTTTTTT65620.1448559365547775No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63060.13920474488180842No Hit
GGAAGCAGTGGTATCAACGCAGAGT61790.13640122401279642No Hit
AAAAAGTACTCTGCGTTGATACCAC58870.1299553335108161No Hit
GAGTACATGGTAAGCAGTGGTATCA58780.12975665880356327No Hit
CTCTGCGTTGATACCACTGCTTCCC47280.1043704462101475No Hit
CTGCGTTGATACCACTGCTTCCCAT47110.1039951717631144No Hit
ACTCTGCGTTGATACCACTGCTTAC46640.10295764829190523No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46370.10236162417014678No Hit
TGGGAAGCAGTGGTATCAACGCAGA46030.10161107527608057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCCG402.7757516E-416.61463710
ACCGTTC500.001507261113.2918568
ACCGTCC500.001507261113.2918568
CCGTTCA550.003084374812.0833719
ACGGTAC752.0457609E-411.4177213
TGCTCGA600.005905945311.07642610
TAGACCG700.001473144410.874025
CGGTGGA1106.097518E-610.3571769
TAGACAG3900.010.2466735
CTGGTCG750.00266298410.1270179
CGTACAC1252.52248E-69.8953583
CCTACAC3900.09.7587363
GGACAGT4500.09.7066656
AAAAGTA13050.09.5513282
TACGCAG1201.6711132E-59.5147675
TCTACGC1304.160296E-69.5147673
GCGATCC800.00454156479.49407914
CTAGACT3200.09.217434
GTCTAGA2800.09.17962551
GTCTAAT2800.09.17962551
Produced by FastQC (version 0.11.5)