FastQCFastQC Report
Wed 25 May 2016
SRR1294744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4427629
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC437080.9871649137721341No Hit
CCCATGTACTCTGCGTTGATACCAC433600.9793051766532381No Hit
GAGTACATGGGAAGCAGTGGTATCA192630.43506355207267816No Hit
CATGTACTCTGCGTTGATACCACTG155190.3505036216900739No Hit
GCGTTGATACCACTGCTTCCCATGT143110.3232203962888489No Hit
GTATCAACGCAGAGTACATGGGAAG110030.2485077227563556No Hit
ACGCAGAGTACATGGGAAGCAGTGG106940.24152881824561181No Hit
ACTCTGCGTTGATACCACTGCTTCC93480.21112880053861782No Hit
TATCAACGCAGAGTACATGGGAAGC92140.2081023500388131No Hit
GCTTCCCATGTACTCTGCGTTGATA84830.19159238499883346No Hit
GGTATCAACGCAGAGTACATGGGAA71630.16177958903060757No Hit
GTGGTATCAACGCAGAGTACATGGG56980.12869190259617505No Hit
CAGTGGTATCAACGCAGAGTACATG56200.1269302373798708No Hit
ATACCACTGCTTCCCATGTACTCTG49290.11132369039953437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT855.3522934E-511.1714348
TCGACCG1106.0840302E-610.3590849
TACGACA1502.449815E-810.1331174
ATAGGAC2900.09.5002253
CTTAGAC3300.09.5002253
TTGCGCA1002.7580158E-49.4997964
TGGACCG2401.8189894E-129.499265
GACCCGT900.00111839439.4962566
GCGGCTA800.00453548139.49561213
GTCCTAG3550.09.3692791
CTAGGAC3500.09.2287913
GGACCGT1451.717468E-69.1687986
CGACGTA950.00183215418.9960469
TAGGACC2750.08.9816264
GTATTAG5250.08.8695831
GTATTAA8400.08.824331
GCACCGT1752.6446105E-78.6822916
CTAGACT3400.08.6615794
TGGCGTA1651.0524054E-68.6356915
CGCCTAA1107.18344E-48.6321814
Produced by FastQC (version 0.11.5)