##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294744_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4427629
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.190789020489294	33.0	33.0	33.0	27.0	33.0
2	31.229172995298388	33.0	33.0	33.0	27.0	33.0
3	31.143096000139128	33.0	33.0	33.0	27.0	33.0
4	34.66212842132889	37.0	37.0	37.0	27.0	37.0
5	34.83947119327297	37.0	37.0	37.0	33.0	37.0
6	35.05822506808949	37.0	37.0	37.0	33.0	37.0
7	35.059829764417934	37.0	37.0	37.0	33.0	37.0
8	35.00091019369509	37.0	37.0	37.0	33.0	37.0
9	34.705331679777146	37.0	37.0	37.0	27.0	37.0
10	34.79949675096987	37.0	37.0	37.0	27.0	37.0
11	35.02321874755089	37.0	37.0	37.0	33.0	37.0
12	34.948924130725494	37.0	37.0	37.0	33.0	37.0
13	34.865324759594806	37.0	37.0	37.0	33.0	37.0
14	36.05096949179798	40.0	37.0	40.0	27.0	40.0
15	36.34978675945975	40.0	37.0	40.0	27.0	40.0
16	36.357829664590234	40.0	37.0	40.0	27.0	40.0
17	36.31932960056048	40.0	37.0	40.0	27.0	40.0
18	36.31921712501205	40.0	37.0	40.0	27.0	40.0
19	36.37833228574481	40.0	37.0	40.0	27.0	40.0
20	36.27312767171775	40.0	37.0	40.0	27.0	40.0
21	36.20759666177993	40.0	37.0	40.0	27.0	40.0
22	36.08475055159319	40.0	37.0	40.0	27.0	40.0
23	35.697768715490845	37.0	37.0	40.0	27.0	40.0
24	36.00976391653411	37.0	37.0	40.0	27.0	40.0
25	34.89397056528449	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5020625456494408
1101	2	0.6652804368206517
1101	3	0.45342293269843026
1101	4	0.5529187381741565
1101	5	0.58577373713063
1101	6	0.5425758029257821
1101	7	0.4881959353146996
1101	8	0.6571973787575445
1101	9	0.8631709896159876
1101	10	0.8252504754600736
1101	11	0.6031668073051435
1101	12	0.6535397050558558
1101	13	0.6807112784369309
1101	14	1.3992443890056876
1101	15	1.0152901419014455
1101	16	1.083655089904937
1101	17	1.0529809667189056
1101	18	1.0316990586617507
1101	19	1.0046206450197346
1101	20	1.1534042233456532
1101	21	1.1273130939152338
1101	22	1.2057684649377904
1101	23	1.5064626110376977
1101	24	1.2479949669104897
1101	25	1.2448830886314823
1102	1	0.17287119568108977
1102	2	0.43686811565712347
1102	3	0.17979084838025372
1102	4	0.18729098738141658
1102	5	0.16770712106627883
1102	6	0.21019390649438918
1102	7	0.23108088870385757
1102	8	0.40052213013609617
1102	9	0.5754693411248226
1102	10	0.5552208592924686
1102	11	0.3564793695050952
1102	12	0.42636970064472024
1102	13	0.40894131590602
1102	14	1.0624174619348992
1102	15	0.8080545517866966
1102	16	0.5154158507266189
1102	17	0.703353277243913
1102	18	0.7568096823001582
1102	19	0.6590386872503586
1102	20	0.7801568572893913
1102	21	0.7750867867101903
1102	22	0.8729787953792254
1102	23	1.2062530024963536
1102	24	0.9153887699630516
1102	25	0.8333368205220637
1103	1	0.2467808113478327
1103	2	0.5002795972673972
1103	3	0.06660474511242498
1103	4	-0.04243503540379123
1103	5	0.1457206431334228
1103	6	0.12028538360065255
1103	7	0.15747362736657067
1103	8	0.31964576984004367
1103	9	0.48150801314738345
1103	10	0.5365866871774045
1103	11	0.2901633520049387
1103	12	0.3299566983220217
1103	13	0.300998603366466
1103	14	0.9974347902413214
1103	15	0.6645571041970939
1103	16	0.3726458465563667
1103	17	0.4741886721947708
1103	18	0.5377888445299348
1103	19	0.5422022358742993
1103	20	0.7171112655892031
1103	21	0.7464859819813938
1103	22	0.6769440490175
1103	23	1.1799402826097705
1103	24	0.8187061555733024
1103	25	0.7281476494211887
1104	1	0.3072860312262691
1104	2	0.500557862715965
1104	3	0.32430665953633664
1104	4	0.33069979155111895
1104	5	0.30221812016981175
1104	6	0.22689389137605076
1104	7	0.1869910232652714
1104	8	0.385034760218133
1104	9	0.5816269327824841
1104	10	0.5584048877435137
1104	11	0.324257578900891
1104	12	0.29815929138874964
1104	13	0.36333387763661307
1104	14	0.8244219057035451
1104	15	0.6409596291523556
1104	16	0.5267695921901066
1104	17	0.6144631323280194
1104	18	0.57380006611384
1104	19	0.6121338037616724
1104	20	0.6607089073107133
1104	21	0.6655069226570376
1104	22	0.8093060240580812
1104	23	1.2020511562771148
1104	24	0.9438087218163673
1104	25	0.819840140392742
1105	1	0.24667214206364108
1105	2	0.3568279450984768
1105	3	0.30907500591261794
1105	4	0.2980833751636638
1105	5	0.2756924795211333
1105	6	0.17731017598259768
1105	7	0.21546632582115421
1105	8	0.32522073832009823
1105	9	-1.6381012701885211
1105	10	-0.10089710084086079
1105	11	0.039886491241347244
1105	12	0.24272510032942307
1105	13	0.3140764833167893
1105	14	-1.6407419752446017
1105	15	-0.27232070267313446
1105	16	-0.0735883622626261
1105	17	0.2314636943396522
1105	18	0.08218785865595635
1105	19	0.3149933816134336
1105	20	0.46854721073725614
1105	21	0.5223687922758771
1105	22	0.5216512389286478
1105	23	0.9928686158257705
1105	24	0.7238919943355313
1105	25	0.38804700667515846
1106	1	0.04956486499168378
1106	2	0.1282018003640708
1106	3	0.155702085450514
1106	4	-1.214586771184436
1106	5	-0.3316908264824008
1106	6	0.004609206561262624
1106	7	0.09396422737719945
1106	8	0.24120770663190427
1106	9	0.4817868564508814
1106	10	0.4777843286246437
1106	11	0.3638625451565147
1106	12	0.12389632099065295
1106	13	0.20113278488286568
1106	14	0.6795150218212527
1106	15	0.44120451667690475
1106	16	0.2702238541055664
1106	17	0.16668840214808256
1106	18	0.2851965855151519
1106	19	0.30365727579675195
1106	20	0.4197572312619613
1106	21	0.6138657705334154
1106	22	0.6418885839819737
1106	23	0.7992781697531512
1106	24	0.4473523770100698
1106	25	0.38813196117481397
1107	1	0.3714496209829683
1107	2	0.31903153494181424
1107	3	0.33622846400641393
1107	4	0.6058895085767588
1107	5	0.46067913670479754
1107	6	0.23200439366708991
1107	7	0.23061617981164773
1107	8	0.20604575200251674
1107	9	0.3909370071768521
1107	10	0.41937686663168705
1107	11	0.1765687683392514
1107	12	0.1913163527669539
1107	13	0.1964200390791504
1107	14	0.7472698883359641
1107	15	0.5979563422701801
1107	16	0.4964641242645058
1107	17	0.5039412112135224
1107	18	0.5648347738142121
1107	19	0.5245175359363827
1107	20	0.679993967141236
1107	21	0.6415115634853947
1107	22	0.5270265083735381
1107	23	0.873541057870014
1107	24	0.9110882533720357
1107	25	0.7010160058799997
1108	1	0.4788283225349801
1108	2	0.4304966495237821
1108	3	0.5191723771175667
1108	4	0.6222002548201075
1108	5	0.5908611302500546
1108	6	0.4564796560517195
1108	7	0.40267083039222484
1108	8	0.4570905028125196
1108	9	0.22097460006761338
1108	10	0.4562504432417569
1108	11	0.3297570036035964
1108	12	0.4314251241180642
1108	13	0.4087294962305492
1108	14	-0.9675836377481062
1108	15	0.05425977409784366
1108	16	0.4420215025653249
1108	17	0.5472771204810272
1108	18	0.5875948816491174
1108	19	0.6053983008369741
1108	20	0.6725993523059657
1108	21	0.7885269950464675
1108	22	0.8763178558078621
1108	23	1.1618970269436204
1108	24	0.9877273215475384
1108	25	0.8874788742529987
1109	1	0.5103611111204351
1109	2	0.3448602999732451
1109	3	0.4574802210372191
1109	4	0.6270384673650753
1109	5	0.6100314004775456
1109	6	0.41098278117407006
1109	7	0.3755920596486675
1109	8	0.42779765598141495
1109	9	0.619413455047443
1109	10	0.5514768740379736
1109	11	0.4036342004150768
1109	12	0.42313128347889517
1109	13	0.3875475143956635
1109	14	0.7619640962778647
1109	15	0.5083466327563428
1109	16	0.5223800898417537
1109	17	0.5299360027343454
1109	18	0.5601643628279902
1109	19	0.5276957759980192
1109	20	0.7130525262856864
1109	21	0.633873507513222
1109	22	0.7499789639246828
1109	23	1.2180063225061417
1109	24	0.8592802945164593
1109	25	0.6455063943228438
1110	1	0.36103681071240246
1110	2	0.20847268893828286
1110	3	0.43462865321318134
1110	4	0.5487195630334512
1110	5	0.46721906944821967
1110	6	0.26845007943758503
1110	7	0.30076833839427763
1110	8	0.29077336244917973
1110	9	0.37814618469396777
1110	10	0.4335748423417769
1110	11	0.3290934446389002
1110	12	0.1588569058300351
1110	13	0.04352483972703425
1110	14	0.5613502381707107
1110	15	0.30222229150038515
1110	16	0.3940022937013907
1110	17	0.4197078890257586
1110	18	0.45337825665674103
1110	19	0.4166102423080389
1110	20	0.5328635069736478
1110	21	0.5728494287987758
1110	22	0.6247319539808132
1110	23	0.7601758886026815
1110	24	0.7249155103127265
1110	25	0.6035212134954904
1111	1	0.24867776597230673
1111	2	0.15734553983026345
1111	3	0.3278088894055493
1111	4	0.591737966634895
1111	5	0.4634093878879071
1111	6	0.31899591688723206
1111	7	0.3020021073169801
1111	8	0.345812770568223
1111	9	0.37921616885972753
1111	10	0.24452742048264042
1111	11	0.25436480557467434
1111	12	0.13513142375348508
1111	13	0.24755738371485592
1111	14	-1.7366668738461541
1111	15	-0.5020176553375961
1111	16	0.01501943135276207
1111	17	0.23377947633391472
1111	18	0.26384772300554005
1111	19	0.3206265379645643
1111	20	0.35513359998745386
1111	21	0.4593429639742439
1111	22	0.2342759753245005
1111	23	-1.8397014404398249
1111	24	-0.4258590657813244
1111	25	0.13248826132750224
1112	1	-0.15122695807151132
1112	2	-0.2753441351557875
1112	3	0.006885928635057326
1112	4	0.1943833777212518
1112	5	-0.6697163783786593
1112	6	-0.8708378846969183
1112	7	-1.0688282166279564
1112	8	-0.8051365481017925
1112	9	-0.7356510895512471
1112	10	-0.9601898751768232
1112	11	-0.8458812683009356
1112	12	-0.7949833283185654
1112	13	-0.8600365643243393
1112	14	-0.6661804386434937
1112	15	-1.0077432325221451
1112	16	-0.8835183566548963
1112	17	-1.0163474357830324
1112	18	-1.013271949875893
1112	19	-0.9071722600617633
1112	20	-0.9257401860959718
1112	21	-1.9188221387013016
1112	22	-1.8523970831606391
1112	23	-1.393094527253865
1112	24	-1.828912306065817
1112	25	-2.0377494274622023
1113	1	0.024891565546319327
1113	2	-0.11602880331288645
1113	3	-0.10853671660518671
1113	4	0.21331629574996924
1113	5	0.11091276438746434
1113	6	-0.013996475112072915
1113	7	0.00834655945391205
1113	8	-0.0013799679715447155
1113	9	0.22606283983923703
1113	10	-0.021456228558129453
1113	11	-0.19094571888933842
1113	12	-0.008745989450616776
1113	13	-0.15239810702199463
1113	14	-0.03186776138318237
1113	15	-0.07978893863442238
1113	16	-0.04021007603770954
1113	17	-0.048398583417828434
1113	18	-0.006148001986609586
1113	19	-0.10331923753320638
1113	20	-0.7073817881492062
1113	21	-0.6141319931810969
1113	22	-0.13850981651666672
1113	23	-0.2396261738193175
1113	24	-0.017616320999295
1113	25	-1.0732262135464197
1114	1	-0.9106295836443898
1114	2	-0.6627164527646734
1114	3	-0.5129978702421703
1114	4	-1.5446235260971264
1114	5	-0.7572945022391337
1114	6	-0.6866862767721429
1114	7	-0.5460324941635619
1114	8	-0.43100408697813464
1114	9	-0.42294329159494737
1114	10	-0.6192739925271482
1114	11	-0.565500138793297
1114	12	-0.5530274074636594
1114	13	-0.6076971212355673
1114	14	-0.6447693934769063
1114	15	-1.4030247645930771
1114	16	-0.9416918478476504
1114	17	-0.8482692985105018
1114	18	-0.7923630536983737
1114	19	-0.8305808221394244
1114	20	-2.004632970757328
1114	21	-6.891172517009192
1114	22	-7.897849046367266
1114	23	-7.945136877047439
1114	24	-5.993816804344885
1114	25	-7.449000849524115
1115	1	-0.9673849277778714
1115	2	-0.9997774576942504
1115	3	-0.6837972175952274
1115	4	-0.5928891899279733
1115	5	-0.5768128246196227
1115	6	-0.927894995968245
1115	7	-0.97779422350564
1115	8	-0.7638170882286417
1115	9	-2.671254874961619
1115	10	-1.63551783292926
1115	11	-1.1845514760937021
1115	12	-1.033698429662934
1115	13	-1.3939509864849526
1115	14	-1.0986840422796433
1115	15	-2.005880961209826
1115	16	-1.347601730814425
1115	17	-3.139348855520595
1115	18	-2.716687487463659
1115	19	-2.0107441744307835
1115	20	-1.85928390645347
1115	21	-1.3327876118153767
1115	22	-0.8603016378798927
1115	23	-0.3326547540032152
1115	24	-1.3282901924423598
1115	25	-1.0021802814501797
1116	1	-1.6562997315205443
1116	2	-0.9605393767344346
1116	3	-0.6650941147980873
1116	4	-0.36515824780590833
1116	5	-0.37796744204642607
1116	6	-0.4229120863934739
1116	7	-0.8419206743544692
1116	8	-1.7509478960007598
1116	9	-0.989614975751941
1116	10	-2.374724468681329
1116	11	-1.1515355516109977
1116	12	-0.766873078650903
1116	13	-0.8022440613932957
1116	14	-1.188174855813699
1116	15	-1.34319762401487
1116	16	-0.8897310239305227
1116	17	-0.7759876203383769
1116	18	-1.2925902966215972
1116	19	-0.9032673948266776
1116	20	-1.222658337930362
1116	21	-0.8301971095180889
1116	22	-0.21948465508823745
1116	23	-0.16839804574870954
1116	24	-0.22818547297299574
1116	25	-0.2674156201408948
1201	1	0.20251006762872947
1201	2	0.3332126347066904
1201	3	0.1751831525937284
1201	4	0.286910001227092
1201	5	0.26484549518519884
1201	6	0.25524168509216594
1201	7	0.22572764087224328
1201	8	0.3833502420913888
1201	9	0.6074358076487556
1201	10	0.6055604701840593
1201	11	0.4131335739355464
1201	12	0.4233370122494122
1201	13	0.43172951664823245
1201	14	1.1765531045187814
1201	15	0.8515083651423652
1201	16	0.800414407041842
1201	17	0.6896572420439213
1201	18	0.625485489497926
1201	19	0.6510951255623851
1201	20	0.850360988170543
1201	21	0.8749414407181817
1201	22	1.029851925960486
1201	23	0.699114890282118
1201	24	0.8030423979810308
1201	25	0.6508028564090296
1202	1	-0.4874491743085656
1202	2	-0.0771354438452505
1202	3	-0.15273539745788867
1202	4	-0.14269258437342813
1202	5	-0.09837442378966443
1202	6	-0.14873391926080615
1202	7	-0.133871390381465
1202	8	0.0686072520289045
1202	9	0.0864079842839729
1202	10	0.2624423570948906
1202	11	0.026871434890239243
1202	12	0.06600003212842864
1202	13	0.165729036913298
1202	14	0.3221318920062046
1202	15	0.30955562272072257
1202	16	0.24102423611589074
1202	17	0.13698927926490967
1202	18	0.053826951511489085
1202	19	0.1337010226342059
1202	20	0.28949153749518075
1202	21	0.2932573498600348
1202	22	0.26608324708198694
1202	23	0.5443527302290008
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2211	5	0.27865312431655553
2211	6	0.1904754867021765
2211	7	0.20802276280181786
2211	8	0.2504008988344779
2211	9	0.3498292214489709
2211	10	0.3339523890118201
2211	11	0.17167612055706627
2211	12	0.1928928070847178
2211	13	0.12697353946483503
2211	14	0.4315958133779745
2211	15	0.26337705566680114
2211	16	0.20625797307970117
2211	17	0.2277474752259181
2211	18	0.2962680498847021
2211	19	0.26362737939999903
2211	20	0.33523423073200576
2211	21	0.34656356140288125
2211	22	0.4403045234167564
2211	23	0.5849501132749637
2211	24	0.5268835109462984
2211	25	0.6115516782383636
2212	1	-0.21768300751713454
2212	2	-0.28913315425930364
2212	3	-0.12899030170320458
2212	4	-0.4974344828901138
2212	5	-0.15253126335564104
2212	6	-0.43638454744627353
2212	7	-0.37448800985028186
2212	8	-0.2734701225401608
2212	9	-0.16620152681492328
2212	10	-0.4639929655343664
2212	11	-0.48823142578338974
2212	12	-0.6036412078028022
2212	13	-0.6054575042093262
2212	14	-0.3997938862731729
2212	15	-0.5756866156735327
2212	16	-0.3300495555294063
2212	17	-0.4472959431400483
2212	18	-0.4329421000520668
2212	19	-0.5447904197454463
2212	20	-0.5344580339533849
2212	21	-0.3848244043567206
2212	22	-0.49691138416113745
2212	23	-0.052212360010393866
2212	24	-0.33631498956187755
2212	25	-0.9339122392585111
2213	1	-0.07582797670416852
2213	2	-1.2236120030784967
2213	3	-0.23826415227724596
2213	4	0.11909891643487214
2213	5	0.11154785736783879
2213	6	0.0050283081635242866
2213	7	-0.03674547301596931
2213	8	0.12122830805692786
2213	9	0.12435489643748099
2213	10	-0.42643270340430917
2213	11	-0.0772218507680762
2213	12	-0.0865707313633024
2213	13	-0.3608559599423913
2213	14	-0.009644677433712445
2213	15	-0.045820081457094375
2213	16	0.003185646348242699
2213	17	0.1302616030599424
2213	18	0.005244032206725535
2213	19	0.05124274650760441
2213	20	0.14804752436479163
2213	21	-0.4698904855031074
2213	22	-0.9182372521887316
2213	23	-0.05721144173031689
2213	24	0.042063892019683635
2213	25	-0.5908347480930658
2214	1	-0.19491733482296425
2214	2	-0.44140574635522256
2214	3	-0.41734399437456915
2214	4	-0.621352693013975
2214	5	-0.31298826700729165
2214	6	-0.21356456187828599
2214	7	-0.5369225294257731
2214	8	-0.18713082318628693
2214	9	-0.07793284274077905
2214	10	-1.9865420768071544
2214	11	-0.9165555102259333
2214	12	-0.5799983487559643
2214	13	-0.39029612096028643
2214	14	-0.9700416001811405
2214	15	-0.7106941584516875
2214	16	-0.8496139136270315
2214	17	-0.6988072870945032
2214	18	-0.6531040254053622
2214	19	-1.5032651948678648
2214	20	-2.593179726016089
2214	21	-1.855729623366166
2214	22	-2.0586928773499693
2214	23	-2.5906836213616486
2214	24	-1.6630116033731994
2214	25	-2.1279353063987756
2215	1	-0.23037763346059492
2215	2	-0.3199646002775296
2215	3	-0.8672392839125962
2215	4	-0.3265073739114399
2215	5	-0.21803298880914213
2215	6	-0.22192111059363384
2215	7	-0.23513178038051308
2215	8	-0.2879559366472648
2215	9	-0.1829431080793853
2215	10	-0.34777490412982814
2215	11	-0.5736085164110918
2215	12	-0.5338574117213284
2215	13	-0.2880216253486836
2215	14	-0.2310436588992033
2215	15	-0.6461618855171594
2215	16	-0.4459272945422086
2215	17	-0.3666028205275751
2215	18	-0.26675970412591
2215	19	-2.307466139438283
2215	20	-2.4423694861852283
2215	21	-3.3070845892631127
2215	22	-2.3405651985493208
2215	23	-3.644657779932402
2215	24	-2.652851272756429
2215	25	-1.9262508949360608
2216	1	-1.5688520402957273
2216	2	-1.2232360538778337
2216	3	-0.8863881373387734
2216	4	-0.9474213503540341
2216	5	-1.8704535746503836
2216	6	-1.6001185181216542
2216	7	-1.076592979065623
2216	8	-5.375998888918421
2216	9	-4.267981141029516
2216	10	-2.114862671317155
2216	11	-1.0556697100252066
2216	12	-1.290187909896531
2216	13	-2.0159205251312002
2216	14	-3.613145393442828
2216	15	-2.163353865360925
2216	16	-3.120188586562591
2216	17	-2.741614340810628
2216	18	-2.7492533222906275
2216	19	-1.7911720874955464
2216	20	-1.0322576056716954
2216	21	-0.7822909460231102
2216	22	-0.7692546780382585
2216	23	-0.9512018511169629
2216	24	-0.5410730273999818
2216	25	-0.4549353304945214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	38.0
8	257.0
9	725.0
10	1765.0
11	3336.0
12	5923.0
13	8908.0
14	11879.0
15	15576.0
16	18385.0
17	19787.0
18	21251.0
19	22196.0
20	23117.0
21	25227.0
22	27181.0
23	29581.0
24	31942.0
25	34762.0
26	37804.0
27	43540.0
28	49220.0
29	59066.0
30	71169.0
31	87336.0
32	117923.0
33	168111.0
34	244145.0
35	387100.0
36	673427.0
37	2186950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10317677813657	19.55139725778572	12.751257998539177	24.59416796553853
2	14.928966799670382	20.40235882812161	39.3741145133076	25.294559858900406
3	19.1660582325652	25.39352433344839	28.87047672920285	26.569940704783555
4	12.816440719203529	17.112021612883332	36.523360153652256	33.548177514260885
5	13.004741713296902	38.2731263003567	35.38013902097965	13.341992965366742
6	32.4321400241047	36.38576738420618	17.38870096617572	13.793391625513399
7	27.049445244346575	32.46715790068039	22.398712628101975	18.084684226871065
8	25.541979391604457	36.85360486564657	20.313428477150104	17.29098726559887
9	25.54515536778352	16.913644462643457	20.72594329512369	36.81525687444933
10	15.07917579121586	30.351751371392645	33.38238907800496	21.18668375938654
11	32.826406052410896	25.02559518257219	22.359721121485702	19.78827764353121
12	22.135194102394998	27.093862082049707	30.398842703430333	20.372101112124962
13	27.808728136744726	22.381942938710612	25.61357148019659	24.19575744434808
14	21.140554959922973	23.018570561606865	26.990384630586465	28.850489847883694
15	24.800108954481917	29.143358937486013	23.055414330811917	23.001117777220156
16	21.853824945618626	29.45382377114809	26.722569958353727	21.969781324879555
17	22.432861504853612	29.311983868195497	27.292007479570422	20.963147147380468
18	22.916446453690227	28.07636398788309	27.986449335656978	21.020740222769703
19	22.431497451559068	29.078154823517615	28.208904625498775	20.281443099424543
20	22.967662929814082	27.881417793609735	27.680858214315073	21.4700610622611
21	22.091651508805274	28.043034387229465	28.222284056702566	21.64303004726269
22	22.402569735447535	27.757179800787185	26.559583808098935	23.280666655666344
23	21.641374733503433	28.040615071364115	27.68435956738498	22.633650627747468
24	22.20508218821971	29.34105553348431	26.792892100675292	21.66097017762069
25	22.504384586898123	27.20945151239953	26.333643295181165	23.952520605521187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4190.0
1	4190.0
2	4839.0
3	5488.0
4	5488.0
5	5488.0
6	7240.0
7	8992.0
8	8992.0
9	8992.0
10	11929.0
11	14866.0
12	14866.0
13	14866.0
14	23144.5
15	31423.0
16	31423.0
17	31423.0
18	49031.0
19	66639.0
20	66639.0
21	66639.0
22	96370.5
23	126102.0
24	126102.0
25	126102.0
26	167266.5
27	208431.0
28	208431.0
29	208431.0
30	255959.0
31	303487.0
32	303487.0
33	303487.0
34	343324.0
35	383161.0
36	383161.0
37	383161.0
38	414821.0
39	446481.0
40	446481.0
41	446481.0
42	474238.5
43	501996.0
44	501996.0
45	501996.0
46	578777.0
47	655558.0
48	655558.0
49	655558.0
50	609834.0
51	564110.0
52	564110.0
53	564110.0
54	486704.5
55	409299.0
56	409299.0
57	409299.0
58	351440.0
59	293581.0
60	293581.0
61	293581.0
62	247217.0
63	200853.0
64	200853.0
65	200853.0
66	156759.5
67	112666.0
68	112666.0
69	112666.0
70	83634.5
71	54603.0
72	54603.0
73	54603.0
74	39006.5
75	23410.0
76	23410.0
77	23410.0
78	15924.0
79	8438.0
80	8438.0
81	8438.0
82	5625.5
83	2813.0
84	2813.0
85	2813.0
86	1801.5
87	790.0
88	790.0
89	790.0
90	493.0
91	196.0
92	196.0
93	196.0
94	117.0
95	38.0
96	38.0
97	38.0
98	28.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030535530415940454
2	0.015471034271389949
3	0.021794960688892407
4	0.022811306005991017
5	0.02165944797994593
6	0.00625617006302922
7	9.034180596432086E-5
8	3.6136722385728343E-4
9	8.582471566610481E-4
10	2.936108693840428E-4
11	8.582471566610481E-4
12	0.0015583961528845348
13	0.0024618142125277433
14	6.775635447324064E-4
15	0.00207786153717938
16	0.002303716052090182
17	0.002303716052090182
18	0.002190788794634781
19	4.517090298216043E-4
20	3.8395267534836367E-4
21	0.08203035981560333
22	0.07941044744263803
23	0.08033645095377231
24	0.0010841016715718503
25	0.2231668461833636
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4427629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.409622148681144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.51983702523353	48.83283145044296
2	12.548784361680365	15.412322121521399
3	3.8288679760552244	7.053880070002105
4	1.562671217671115	3.838521960792105
5	0.7949951524304711	2.4410175960394196
6	0.4192611709013009	1.5448002051997507
7	0.28590362362372906	1.2290063448370365
8	0.18807602075417984	0.9239741895793357
9	0.1439692324094512	0.7956986546970052
>10	0.6480112524790989	6.980055278964612
>50	0.03878657661359302	1.6353982443545585
>100	0.01782543925175659	1.9414132148090766
>500	0.001115167149633706	0.47946523881563796
>1k	0.0014125447522459749	2.248902521107319
>5k	2.2303338193354328E-4	1.0392368519044846
>10k+	2.602056122558004E-4	3.60347605693314
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	43708	0.9871649137721341	No Hit
CCCATGTACTCTGCGTTGATACCAC	43360	0.9793051766532381	No Hit
GAGTACATGGGAAGCAGTGGTATCA	19263	0.43506355207267816	No Hit
CATGTACTCTGCGTTGATACCACTG	15519	0.3505036216900739	No Hit
GCGTTGATACCACTGCTTCCCATGT	14311	0.3232203962888489	No Hit
GTATCAACGCAGAGTACATGGGAAG	11003	0.2485077227563556	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	10694	0.24152881824561181	No Hit
ACTCTGCGTTGATACCACTGCTTCC	9348	0.21112880053861782	No Hit
TATCAACGCAGAGTACATGGGAAGC	9214	0.2081023500388131	No Hit
GCTTCCCATGTACTCTGCGTTGATA	8483	0.19159238499883346	No Hit
GGTATCAACGCAGAGTACATGGGAA	7163	0.16177958903060757	No Hit
GTGGTATCAACGCAGAGTACATGGG	5698	0.12869190259617505	No Hit
CAGTGGTATCAACGCAGAGTACATG	5620	0.1269302373798708	No Hit
ATACCACTGCTTCCCATGTACTCTG	4929	0.11132369039953437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.2585451491080214E-5	0.0
4	0.0	0.0	0.0	2.2585451491080214E-5	0.0
5	0.0	0.0	0.0	2.2585451491080214E-5	0.0
6	0.0	0.0	0.0	2.2585451491080214E-5	0.0
7	0.0	0.0	0.0	2.2585451491080214E-5	0.0
8	0.0	0.0	0.0	2.2585451491080214E-5	0.0
9	0.0	0.0	0.0	2.2585451491080214E-5	0.0
10	0.0	0.0	0.0	2.2585451491080214E-5	0.0
11	0.0	0.0	0.0	6.775635447324064E-5	0.0
12	0.0	0.0	0.0	6.775635447324064E-5	0.0
13	0.0	0.0	0.0	9.034180596432086E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGT	85	5.3522934E-5	11.171434	8
TCGACCG	110	6.0840302E-6	10.359084	9
TACGACA	150	2.449815E-8	10.133117	4
ATAGGAC	290	0.0	9.500225	3
CTTAGAC	330	0.0	9.500225	3
TTGCGCA	100	2.7580158E-4	9.499796	4
TGGACCG	240	1.8189894E-12	9.49926	5
GACCCGT	90	0.0011183943	9.496256	6
GCGGCTA	80	0.0045354813	9.495612	13
GTCCTAG	355	0.0	9.369279	1
CTAGGAC	350	0.0	9.228791	3
GGACCGT	145	1.717468E-6	9.168798	6
CGACGTA	95	0.0018321541	8.996046	9
TAGGACC	275	0.0	8.981626	4
GTATTAG	525	0.0	8.869583	1
GTATTAA	840	0.0	8.82433	1
GCACCGT	175	2.6446105E-7	8.682291	6
CTAGACT	340	0.0	8.661579	4
TGGCGTA	165	1.0524054E-6	8.635691	5
CGCCTAA	110	7.18344E-4	8.63218	14
>>END_MODULE