FastQCFastQC Report
Wed 25 May 2016
SRR1294744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4427629
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC473411.0692178590392283No Hit
GTACATGGGAAGCAGTGGTATCAAC421690.9524059039273616No Hit
GAGTACATGGGAAGCAGTGGTATCA204310.4614433594142599No Hit
CATGTACTCTGCGTTGATACCACTG177490.4008691785151827No Hit
GCGTTGATACCACTGCTTCCCATGT152150.34363764443678546No Hit
ACGCAGAGTACATGGGAAGCAGTGG134830.3045196424542345No Hit
GTATCAACGCAGAGTACATGGGAAG117640.26569525134106764No Hit
GTATCAACGCAGAGTACTTTTTTTT107680.24320014165595175No Hit
TATCAACGCAGAGTACATGGGAAGC106490.24051247292851322No Hit
ACTCTGCGTTGATACCACTGCTTCC106170.23978973848079863No Hit
GCTTCCCATGTACTCTGCGTTGATA97540.2202984938439964No Hit
GGTATCAACGCAGAGTACATGGGAA79850.1803448301562755No Hit
TATCAACGCAGAGTACTTTTTTTTT76650.17311748567912985No Hit
GGTATCAACGCAGAGTACTTTTTTT73650.16634185023180578No Hit
CAGTGGTATCAACGCAGAGTACATG67460.15236145575882712No Hit
GTGGTATCAACGCAGAGTACATGGG62070.1401878974051349No Hit
ATACCACTGCTTCCCATGTACTCTG54600.12331656514129798No Hit
CATGGGAAGCAGTGGTATCAACGCA51610.116563515145465No Hit
GCAGTGGTATCAACGCAGAGTACAT51060.11532131531345557No Hit
ACATGGGAAGCAGTGGTATCAACGC47870.10811655628780098No Hit
GGGAAGCAGTGGTATCAACGCAGAG45320.10235726615757552No Hit
TACCACTGCTTCCCATGTACTCTGC44300.10005355010548535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGG855.3600455E-511.169847513
TCTAGAC3250.011.1238473
TACACCG1303.2025127E-810.9774825
ACACCGC909.5664254E-510.550856
GTACCGT1303.8704457E-710.2262096
TCGACCG951.6548751E-49.99439
TCTACGC1054.043774E-59.9668563
GTCGTAC1002.70483E-49.5182331
ACGGGTC1401.0391068E-69.5138185
CTAGTAC2800.09.5138183
TACGACA1304.165511E-69.5138184
CCTAGCG800.00446438749.5138173
TAGACCG800.00446438749.5138175
ATCCCGT800.00453488259.4957656
ACGTCGC900.00111892399.4957656
ACTGTCG1304.27361E-69.4946938
AAGACCG1451.6749145E-69.1857545
CGACACT950.0018008719.0130894
TAGACAG6350.08.9894335
CACGGGT1804.1267413E-88.9852714
Produced by FastQC (version 0.11.5)