FastQCFastQC Report
Wed 25 May 2016
SRR1294743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1354644.3048996739482766No Hit
CCCATGTACTCTGCGTTGATACCAC1285724.085879354506568No Hit
GAGTACATGGGAAGCAGTGGTATCA642502.04179563611865No Hit
CATGTACTCTGCGTTGATACCACTG507381.6123988635858062No Hit
GCGTTGATACCACTGCTTCCCATGT408761.2989951505367459No Hit
GCTTCCCATGTACTCTGCGTTGATA389881.2389965488092438No Hit
GTATCAACGCAGAGTACATGGGAAG335591.066468789922269No Hit
ACGCAGAGTACATGGGAAGCAGTGG328921.0452722500111227No Hit
ACTCTGCGTTGATACCACTGCTTCC267310.8494823213865778No Hit
TATCAACGCAGAGTACATGGGAAGC264390.8402028766278751No Hit
GGTATCAACGCAGAGTACATGGGAA218350.6938927270762758No Hit
CAGTGGTATCAACGCAGAGTACATG173930.5527307626305319No Hit
GTGGTATCAACGCAGAGTACATGGG164750.5235577136973503No Hit
GCAGTGGTATCAACGCAGAGTACAT144650.45968208368025326No Hit
ATACCACTGCTTCCCATGTACTCTG137500.4369601555895943No Hit
GTACATGGTAAGCAGTGGTATCAAC132840.42215117868015783No Hit
ACATGGGAAGCAGTGGTATCAACGC126000.400414397122101No Hit
GTACTCTGCGTTGATACCACTGCTT125980.40035083928128795No Hit
CTGCTTCCCATGTACTCTGCGTTGA123900.3937408238367326No Hit
CATGGGAAGCAGTGGTATCAACGCA121890.3873532608350229No Hit
GGGAAGCAGTGGTATCAACGCAGAG120140.38179194976388264No Hit
TACCACTGCTTCCCATGTACTCTGC105280.33456847403979995No Hit
CTTCCCATGTACTCTGCGTTGATAC105240.33444135835817385No Hit
GATACCACTGCTTCCCATGTACTCT101900.32382719894239753No Hit
AAGCAGTGGTATCAACGCAGAGTAC99920.3175349727019074No Hit
GTTGATACCACTGCTTCCCATGTAC99770.3170582888958096No Hit
CCACTGCTTCCCATGTACTCTGCGT98020.31149697782466934No Hit
CCATGTACTCTGCGTTGATACCACT97450.30968557936149793No Hit
GCAGAGTACATGGGAAGCAGTGGTA96240.3058403299923095No Hit
ACCATGTACTCTGCGTTGATACCAC92220.2930652039888901No Hit
ATGGGAAGCAGTGGTATCAACGCAG86120.27368006254091537No Hit
GTACATGGGTAAGCAGTGGTATCAA79160.25156193393798026No Hit
TTCCCATGTACTCTGCGTTGATACC78520.24952808303196325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA69500.22086349682528586No Hit
TGATACCACTGCTTCCCATGTACTC68940.21908387728252096No Hit
CCCCATGTACTCTGCGTTGATACCA64220.20408422685064542No Hit
GGTAAGCAGTGGTATCAACGCAGAG58720.18660582062706166No Hit
GCTTACCATGTACTCTGCGTTGATA54640.17364002110120316No Hit
ATCAACGCAGAGTACATGGGAAGCA51740.16442413418331353No Hit
GAGTACATGGTAAGCAGTGGTATCA44070.14004970223151578No Hit
GGAAGCAGTGGTATCAACGCAGAGT42230.13420238087671685No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA38210.12142725487329743No Hit
AAAAAGTACTCTGCGTTGATACCAC35910.11411810317979879No Hit
TCCATGTACTCTGCGTTGATACCAC34340.10912881267597578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAT504.567266E-617.0992263
GTACAAA7300.013.4075541
TATTCCG604.1000522E-412.6644855
CTAGTAC1001.4450961E-712.3494423
TATCGAC1354.5893103E-911.28170619
GTCCTAC1201.272274E-711.0861821
CGTGCAC1053.4857421E-610.85285710
GGTATCG1252.228262E-710.64493417
CCTACAC2500.010.6395193
GTCTTAG1701.382432E-1010.6203761
AAGCGGT1909.094947E-1210.49585410
AGCGGTG1909.094947E-1210.49585411
TAGGACA2550.010.4300734
GAAGCGG1752.382876E-1010.3103789
GAACAAA16000.010.2151241
TAACCCG750.0026526510.1315875
GACAGCG750.002657550410.12933357
CTGGACA3250.09.93722254
CGCAAAA3200.09.7967442
GTCCTGG3800.09.7525051
Produced by FastQC (version 0.11.5)