FastQCFastQC Report
Wed 25 May 2016
SRR1294743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1383604.396931427445547No Hit
GTACATGGGAAGCAGTGGTATCAAC1343404.269180167411353No Hit
GAGTACATGGGAAGCAGTGGTATCA694622.207427369277411No Hit
CATGTACTCTGCGTTGATACCACTG567481.803390175228967No Hit
GCTTCCCATGTACTCTGCGTTGATA428381.3613453923743304No Hit
GCGTTGATACCACTGCTTCCCATGT427551.3587077419805895No Hit
ACGCAGAGTACATGGGAAGCAGTGG402211.278179957670478No Hit
GTATCAACGCAGAGTACATGGGAAG354841.1276432117048119No Hit
TATCAACGCAGAGTACATGGGAAGC303020.9629648461582463No Hit
ACTCTGCGTTGATACCACTGCTTCC302320.9607403217297903No Hit
GGTATCAACGCAGAGTACATGGGAA245860.7813165371146011No Hit
CAGTGGTATCAACGCAGAGTACATG213750.6792744236892784No Hit
GTGGTATCAACGCAGAGTACATGGG174520.5546057189345164No Hit
GCAGTGGTATCAACGCAGAGTACAT166290.5284516674399538No Hit
CATGGGAAGCAGTGGTATCAACGCA162470.5163121198446646No Hit
ACATGGGAAGCAGTGGTATCAACGC159910.5081767162205966No Hit
ATACCACTGCTTCCCATGTACTCTG149610.4754444282018851No Hit
GGGAAGCAGTGGTATCAACGCAGAG140950.4479238831298423No Hit
CTGCTTCCCATGTACTCTGCGTTGA133390.4238990193025162No Hit
GTACATGGTAAGCAGTGGTATCAAC128440.40816845370129085No Hit
GTACTCTGCGTTGATACCACTGCTT123180.39145274156746346No Hit
AAGCAGTGGTATCAACGCAGAGTAC121850.3872261451533968No Hit
CTTCCCATGTACTCTGCGTTGATAC120360.382491086012826No Hit
GATACCACTGCTTCCCATGTACTCT116240.3693981708053414No Hit
TACCACTGCTTCCCATGTACTCTGC114220.3629788288832252No Hit
GCAGAGTACATGGGAAGCAGTGGTA113450.36053185201192345No Hit
CCACTGCTTCCCATGTACTCTGCGT112460.3573857388916784No Hit
CCATGTACTCTGCGTTGATACCACT111570.3545574149754985No Hit
GTTGATACCACTGCTTCCCATGTAC104000.33050077222776586No Hit
ATGGGAAGCAGTGGTATCAACGCAG103110.327672448311586No Hit
ACCATGTACTCTGCGTTGATACCAC101320.3219840215588196No Hit
GTACATGGGTAAGCAGTGGTATCAA88640.2816883504833574No Hit
TTCCCATGTACTCTGCGTTGATACC81340.25848973858660074No Hit
GTATCAACGCAGAGTACTTTTTTTT76770.24396677196082292No Hit
GGTAAGCAGTGGTATCAACGCAGAG71640.22766418579228026No Hit
CCCCATGTACTCTGCGTTGATACCA70990.2255985559658567No Hit
TGATACCACTGCTTCCCATGTACTC70080.22270667420886378No Hit
ATCAACGCAGAGTACATGGGAAGCA61170.19439165612665807No Hit
TATCAACGCAGAGTACTTTTTTTTT61040.19397853016137337No Hit
GCTTACCATGTACTCTGCGTTGATA59090.18778164068210274No Hit
GGTATCAACGCAGAGTACTTTTTTT58740.18666937846787468No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51660.1641699028200614No Hit
GAGTACATGGTAAGCAGTGGTATCA50860.1616275891875401No Hit
GGAAGCAGTGGTATCAACGCAGAGT49780.15819546578363639No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA45420.14433985648639544No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43530.13833364052956393No Hit
AAAAAGTACTCTGCGTTGATACCAC39040.12406490526703827No Hit
TGGGAAGCAGTGGTATCAACGCAGA36930.1173595530612634No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35450.11265627284109904No Hit
ATGGTAAGCAGTGGTATCAACGCAG35180.11179824199012312No Hit
TCCATGTACTCTGCGTTGATACCAC34460.10951015972085396No Hit
CTGCGTTGATACCACTGCTTCCCAT34160.10855679210865848No Hit
ACTCTGCGTTGATACCACTGCTTAC34090.10833433966581288No Hit
GCGTTGATACCACTGCTTACCATGT32070.10191499774369664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC1251.7955244E-811.4165284
GTATTAA4650.011.0522991
GTACCGG803.7102372E-410.7069141
GTACTTG4350.010.50180151
TAGACAG3000.010.4651495
GGACTGT2500.010.2551176
ATTAACG1409.496034E-810.1933273
CTCCTAG2500.09.8979481
GTAAGAC2054.0017767E-119.7458163
TATACTG4400.09.7299955
CTAGGAC2350.09.7161933
CTAGACA2900.09.5137724
CTAGGGT1401.0387648E-69.5137724
ATACTGT5250.09.4050466
GTACTAG1554.1535532E-79.210251
TAACGCA1451.6743652E-69.1857115
TAGGACT2501.8189894E-129.1332224
AAAAGTA8950.09.035572
TAGAACC1804.1247404E-88.98522954
ATAACGC850.00735042158.9541393
Produced by FastQC (version 0.11.5)