FastQCFastQC Report
Wed 25 May 2016
SRR1294742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276280
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83090.2536108024955132No Hit
CCCATGTACTCTGCGTTGATACCAC74270.22669002649346207No Hit
GTATCAACGCAGAGTACTTTTTTTT46160.1408914988950883No Hit
GAGTACATGGGAAGCAGTGGTATCA38770.11833542920629495No Hit
ATTCCATTCCATTCCATTCCATTCC36200.11049116681113946No Hit
GGTATCAACGCAGAGTACTTTTTTT34540.10542444479714798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT400.00529470714.2443336
CGACTAA500.001494315913.30689817
TAGGCCG500.001502765513.2969445
TGGACCG802.8753871E-511.8722725
TAGACAG4700.011.5186295
CCGGAAC700.00149388810.8556423
GAACCGC909.560006E-510.5513586
TTAAGCG750.002656991410.12961312
GTCTTAC3100.010.1132041
GTCGTAC856.5952935E-410.0591231
CTAGACA3900.09.4985434
CGGGACT800.00452342779.4985424
TACATCG800.00452627669.4978175
TGCACCG800.00452627669.4978175
CGCGCGC800.0045319799.496366510
GTCTAGC2054.2382453E-109.2685681
TAAGACT3250.09.0601484
ACGGTAC1054.5010433E-49.0463693
CGCTCAG1054.5120443E-49.0441610
TGGACTG4950.09.0181295
Produced by FastQC (version 0.11.5)