##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294742_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3276280
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15384185722832	33.0	33.0	33.0	27.0	33.0
2	31.16185490861587	33.0	33.0	33.0	27.0	33.0
3	31.11158417473476	33.0	33.0	33.0	27.0	33.0
4	34.6326831040082	37.0	37.0	37.0	27.0	37.0
5	34.877743965717215	37.0	37.0	37.0	33.0	37.0
6	35.01735199677683	37.0	37.0	37.0	33.0	37.0
7	35.00819984860879	37.0	37.0	37.0	33.0	37.0
8	34.919452244618896	37.0	37.0	37.0	33.0	37.0
9	34.65427893830808	37.0	37.0	37.0	27.0	37.0
10	34.74279945548	37.0	37.0	37.0	27.0	37.0
11	34.94017849512252	37.0	37.0	37.0	33.0	37.0
12	34.867827230883805	37.0	37.0	37.0	33.0	37.0
13	34.79550282637626	37.0	37.0	37.0	33.0	37.0
14	35.99848944534655	40.0	37.0	40.0	27.0	40.0
15	36.30031956975594	40.0	37.0	40.0	27.0	40.0
16	36.317895295884355	40.0	37.0	40.0	27.0	40.0
17	36.27470210116351	40.0	37.0	40.0	27.0	40.0
18	36.284201899715534	40.0	37.0	40.0	27.0	40.0
19	36.37826956182011	40.0	37.0	40.0	27.0	40.0
20	36.26080737910069	40.0	37.0	40.0	27.0	40.0
21	36.21319850562223	40.0	37.0	40.0	27.0	40.0
22	36.07975050972444	40.0	37.0	40.0	27.0	40.0
23	35.71142362679625	37.0	37.0	40.0	27.0	40.0
24	36.010070567839136	37.0	37.0	40.0	27.0	40.0
25	34.88131875175504	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5040161945517987
1101	2	0.6629015813817318
1101	3	0.4330027665840319
1101	4	0.5820822991102546
1101	5	0.5689126564496618
1101	6	0.4640481680757702
1101	7	0.4872822014226017
1101	8	0.5765515913441916
1101	9	0.8133472252478597
1101	10	0.7470317357399807
1101	11	0.5752050415649919
1101	12	0.5233247653416413
1101	13	0.5724228483696052
1101	14	1.1857908617796014
1101	15	0.9867533085278595
1101	16	0.9083116009100678
1101	17	0.9455056179511274
1101	18	0.9106285463094963
1101	19	0.9779206744813962
1101	20	1.0035945130917625
1101	21	1.0138560769121412
1101	22	1.0657259754843196
1101	23	1.4070615498173638
1101	24	1.2332023525588838
1101	25	1.0837985150785059
1102	1	0.26006521304766395
1102	2	0.40356391294459115
1102	3	0.24507777548492626
1102	4	0.1671008176175377
1102	5	0.23765411568660255
1102	6	0.27409181227215385
1102	7	0.2642939801192128
1102	8	0.32257083506448936
1102	9	0.4231305852990346
1102	10	0.541969639755095
1102	11	0.3181007138002485
1102	12	0.3112984813739388
1102	13	0.2926678559340985
1102	14	0.9993080073961877
1102	15	0.6072922224632507
1102	16	0.4790811293477404
1102	17	0.6248150097527656
1102	18	0.5890417228253781
1102	19	0.640734108733966
1102	20	0.7078060658340775
1102	21	0.749158706259152
1102	22	0.7963724421280602
1102	23	1.1516934875351765
1102	24	0.7617295070045884
1102	25	0.8205331722339011
1103	1	0.29713539070309736
1103	2	0.5345468733149694
1103	3	0.30213376134230074
1103	4	0.13669167585856457
1103	5	0.3189023812622622
1103	6	0.21952811735832967
1103	7	0.2637754010966731
1103	8	0.3468166413599434
1103	9	0.6675448673022757
1103	10	0.6780169882044476
1103	11	0.42822144887976776
1103	12	0.3755782173927926
1103	13	0.35608975600263904
1103	14	0.9582318007457147
1103	15	0.7219993145936598
1103	16	0.4554657516619116
1103	17	0.5919392520725779
1103	18	0.6664177355427725
1103	19	0.7596159514347534
1103	20	0.7805786316898065
1103	21	0.8660118594400785
1103	22	0.7910292160514274
1103	23	1.3512085238480012
1103	24	0.9365969249377244
1103	25	0.8548836516051139
1104	1	0.3384659875967557
1104	2	0.5581621903381553
1104	3	0.4093958229133037
1104	4	0.42687227541164674
1104	5	0.3217612507498018
1104	6	0.23776672725690418
1104	7	0.3492680186541506
1104	8	0.5268910030163241
1104	9	0.6973597481167744
1104	10	0.6743224302862387
1104	11	0.4316969163094413
1104	12	0.4583529168453566
1104	13	0.43071018628574365
1104	14	1.0068858893001718
1104	15	0.8089597829783912
1104	16	0.695286034094579
1104	17	0.8059904613455373
1104	18	0.7713159802485521
1104	19	0.8084353875650692
1104	20	0.8866646462512406
1104	21	0.9671513478035934
1104	22	0.9867691813050712
1104	23	1.36719630769187
1104	24	1.0709981277017349
1104	25	0.8962873182658271
1105	1	0.17378016253822182
1105	2	0.3009419621668421
1105	3	0.2429809635085256
1105	4	0.18156284316327032
1105	5	0.251267635746693
1105	6	0.19500173086296257
1105	7	0.19905203470273847
1105	8	0.2934822026471551
1105	9	-1.7959429776029125
1105	10	-0.22345355039369252
1105	11	0.0018266031793814363
1105	12	0.20716945830840672
1105	13	0.18320449007089934
1105	14	-1.744160058030623
1105	15	-0.36039948519781007
1105	16	-0.037444622860164145
1105	17	0.03455018233977114
1105	18	0.11431069194011201
1105	19	0.2731972017037023
1105	20	0.31148022620367044
1105	21	0.4386302106266484
1105	22	0.5509731120452983
1105	23	0.7805596997013922
1105	24	0.6087124644452828
1105	25	0.2956613476391965
1106	1	0.18877298657217523
1106	2	0.19770205511331085
1106	3	0.22361948250141594
1106	4	-0.9786031032955762
1106	5	-0.045240931184984845
1106	6	-0.044565863734284505
1106	7	0.025418544598132087
1106	8	0.19505404439792784
1106	9	0.4203893056925452
1106	10	0.4440118239789257
1106	11	0.38716390295980574
1106	12	0.26028409195877344
1106	13	0.27642660490658955
1106	14	0.7052164962588137
1106	15	0.4001488748075346
1106	16	0.28725796704831197
1106	17	0.0364739189731651
1106	18	0.24530185654212033
1106	19	0.2901795325397245
1106	20	0.3906665300801251
1106	21	0.5129569476853533
1106	22	0.6085592195578187
1106	23	0.8143463902346326
1106	24	0.5901771089811874
1106	25	0.5726545346330951
1107	1	0.3223959754886252
1107	2	0.2859111911876475
1107	3	0.27534348899794736
1107	4	0.3763679373358926
1107	5	0.33780596850795774
1107	6	0.17247556797326524
1107	7	0.17508700375956465
1107	8	0.21555938054103052
1107	9	0.3643619043890567
1107	10	0.3367420461209605
1107	11	0.13921654761445268
1107	12	0.18025494396487574
1107	13	0.21219097058490632
1107	14	0.6437169070320792
1107	15	0.4732823486553599
1107	16	0.5531802444073435
1107	17	0.43862759059028633
1107	18	0.500311130459572
1107	19	0.4105524204487665
1107	20	0.52234628682573
1107	21	0.5228522537676312
1107	22	0.5024143525426084
1107	23	0.7187589727386836
1107	24	0.6730400354186656
1107	25	0.563778782843741
1108	1	0.5214682071016021
1108	2	0.4622916186308572
1108	3	0.6032691624899478
1108	4	0.7440829763104801
1108	5	0.6969941714012862
1108	6	0.49728775627794874
1108	7	0.3914339306978647
1108	8	0.48276613119340794
1108	9	0.20193706687866353
1108	10	0.4460980345449883
1108	11	0.4638837068664614
1108	12	0.44657138510967087
1108	13	0.4084505386284434
1108	14	-1.0097175674408163
1108	15	0.12954168843820213
1108	16	0.5053145793535663
1108	17	0.5307054968940932
1108	18	0.7625473652492687
1108	19	0.646792684689288
1108	20	0.7636128794530421
1108	21	0.7618643544610322
1108	22	0.8446387029298279
1108	23	1.1189562048271995
1108	24	1.0112547454101986
1108	25	0.8669446195291499
1109	1	0.35086416628038464
1109	2	0.31192500770240983
1109	3	0.4388207910630513
1109	4	0.540192858637468
1109	5	0.47663926376516486
1109	6	0.3062965922900389
1109	7	0.29258630880038083
1109	8	0.2933243359416906
1109	9	0.4346585388502078
1109	10	0.5529129876477583
1109	11	0.3476087872337672
1109	12	0.25800230428367144
1109	13	0.36811700346741816
1109	14	0.7306526239125049
1109	15	0.5460555141632639
1109	16	0.47813870977361006
1109	17	0.3597516314488587
1109	18	0.47433492459599336
1109	19	0.46740846046798623
1109	20	0.7034429687284316
1109	21	0.5677239018347109
1109	22	0.6739011406842295
1109	23	1.0505646992023756
1109	24	0.8081475488165282
1109	25	0.6968151037474968
1110	1	0.3494707519352147
1110	2	0.2017465779897556
1110	3	0.4315311614124866
1110	4	0.5832435747025997
1110	5	0.49243248933095174
1110	6	0.39452515710466685
1110	7	0.4060414604631717
1110	8	0.34273760801144704
1110	9	0.47758767884079134
1110	10	0.5389081865143623
1110	11	0.27568779104569785
1110	12	0.30362585720313007
1110	13	0.18524438064978455
1110	14	0.6277375795686382
1110	15	0.48603686053529316
1110	16	0.5212558216285359
1110	17	0.5118092055710122
1110	18	0.5292603930788573
1110	19	0.48598268266149347
1110	20	0.5689697363256769
1110	21	0.6410807069769788
1110	22	0.6786177091703678
1110	23	0.812487790446788
1110	24	0.7623936717285957
1110	25	0.722889807086176
1111	1	0.26773799802524323
1111	2	0.16628175586728489
1111	3	0.32013153094223057
1111	4	0.6228459781532294
1111	5	0.431673574307716
1111	6	0.3673476458220435
1111	7	0.22772412336299652
1111	8	0.4361443163733796
1111	9	0.47121415684166834
1111	10	0.3567872306502835
1111	11	0.2815922581802468
1111	12	0.31630473639371814
1111	13	0.32297868592778656
1111	14	-1.6406968952665153
1111	15	-0.3279342929279849
1111	16	0.18384890989701574
1111	17	0.24326033534083535
1111	18	0.44635938885151205
1111	19	0.3762916717099074
1111	20	0.5364120420149163
1111	21	0.5360971171482447
1111	22	0.3566817341470312
1111	23	-1.7495876277263065
1111	24	-0.22861802224786487
1111	25	0.19263914281675198
1112	1	-0.2565290629643471
1112	2	-0.3207858886638135
1112	3	-0.0871323768372747
1112	4	0.07077113399967772
1112	5	-0.6594480543083066
1112	6	-0.8597800193790874
1112	7	-0.9105602564348558
1112	8	-0.7537533485883543
1112	9	-0.7262743357323913
1112	10	-0.8835519226948989
1112	11	-0.8141705705373994
1112	12	-0.7788080013984242
1112	13	-0.8066507808027907
1112	14	-0.6205466747125428
1112	15	-0.9411646732376724
1112	16	-0.817908358436874
1112	17	-0.8473736601276443
1112	18	-1.028263811730021
1112	19	-0.8177979755132299
1112	20	-0.9363726427856349
1112	21	-1.7838015109798846
1112	22	-1.6639690731218906
1112	23	-1.3291745870842107
1112	24	-1.7041623736480815
1112	25	-1.915644812000167
1113	1	-0.02197580522141962
1113	2	-0.13519346218226502
1113	3	-0.07797410995688026
1113	4	0.12526892278699364
1113	5	-0.040612902376004456
1113	6	-0.166691738346195
1113	7	-0.10205771579473577
1113	8	-0.11190667773789897
1113	9	0.07128293219150095
1113	10	-0.23245091729707212
1113	11	-0.2811072818209368
1113	12	-0.2736374628857803
1113	13	-0.2960625411783795
1113	14	-0.14840022489133275
1113	15	-0.19734254197096135
1113	16	-0.2262575956927293
1113	17	-0.22384573149009412
1113	18	-0.1108867903061963
1113	19	-0.29274161397489706
1113	20	-0.8757279956134596
1113	21	-0.8034790033561308
1113	22	-0.15891963140018106
1113	23	-0.41107724219576625
1113	24	-0.08940347220578104
1113	25	-1.3171051509607565
1114	1	-0.8628933270312231
1114	2	-0.7122354075994899
1114	3	-0.5120329254190779
1114	4	-1.4673010180917814
1114	5	-0.750846442072806
1114	6	-0.6211885559622061
1114	7	-0.5268510088446021
1114	8	-0.3760513684812139
1114	9	-0.4060803616902149
1114	10	-0.4507582622228341
1114	11	-0.5749104573852648
1114	12	-0.6088236209205746
1114	13	-0.6034898274700353
1114	14	-0.5664779495523575
1114	15	-1.2688235402195787
1114	16	-0.8214031205331622
1114	17	-0.8260288476559126
1114	18	-0.693124568685171
1114	19	-0.6922439285381685
1114	20	-1.9130835555489227
1114	21	-6.853972979196538
1114	22	-8.044519652983283
1114	23	-8.029732090113558
1114	24	-5.803954457422105
1114	25	-7.524440184463284
1115	1	-1.1205373458972865
1115	2	-1.0371037290167031
1115	3	-0.8355011393410301
1115	4	-0.5596224371724148
1115	5	-0.76169335517136
1115	6	-0.8476643571944891
1115	7	-1.0266798521717746
1115	8	-0.6646618665521942
1115	9	-2.6498086699455996
1115	10	-1.5219011148968207
1115	11	-1.0731059392381823
1115	12	-1.0113636886626693
1115	13	-1.2996896124016928
1115	14	-0.9892665637077869
1115	15	-1.9267590506886165
1115	16	-1.154656095698364
1115	17	-2.9810918130014983
1115	18	-2.7522726029824938
1115	19	-2.132395937763434
1115	20	-1.7759693963437257
1115	21	-1.3272822652572742
1115	22	-0.9223574823665004
1115	23	-0.19019448626495716
1115	24	-1.2418896005988032
1115	25	-1.0256640383263829
1116	1	-1.5313781631624437
1116	2	-0.8035384965482066
1116	3	-0.6155458024854745
1116	4	-0.29517805078271664
1116	5	-0.30560699710709116
1116	6	-0.2803127979613649
1116	7	-0.5944919205224792
1116	8	-1.5540694163724567
1116	9	-0.7683781197956989
1116	10	-2.4623250877950866
1116	11	-1.0734980583838265
1116	12	-0.8585842353398263
1116	13	-0.7406400300048404
1116	14	-1.087041676513799
1116	15	-1.0895556797119568
1116	16	-0.8102025557019559
1116	17	-0.729295245046572
1116	18	-1.3588906972385146
1116	19	-0.963023929096714
1116	20	-1.1827820276221033
1116	21	-0.49253935248011516
1116	22	-0.23555127242392615
1116	23	-0.13952958098673207
1116	24	-0.140956069161966
1116	25	-0.23953932363819774
1201	1	0.30297718183213007
1201	2	0.4515929549611819
1201	3	0.3658391934887142
1201	4	0.5069853855102693
1201	5	0.49821635726937075
1201	6	0.4211003203508241
1201	7	0.44433674325805583
1201	8	0.5505754370063087
1201	9	0.7483990135928664
1201	10	0.7054725013854011
1201	11	0.5508470178732168
1201	12	0.5989872082346324
1201	13	0.590165015679375
1201	14	1.2048996983301237
1201	15	0.9179854244107091
1201	16	0.895910260382621
1201	17	0.7897544299712251
1201	18	0.7149421090440811
1201	19	0.7047590738088729
1201	20	0.9620275923179165
1201	21	0.9059080844196643
1201	22	1.057201099304372
1201	23	0.9129401670791282
1201	24	0.782594346838593
1201	25	0.7992164087678404
1202	1	-0.31083214761365596
1202	2	0.04414739781832111
1202	3	-0.05278242578004466
1202	4	-0.0658392351740602
1202	5	0.041435501454124335
1202	6	0.015345455882943781
1202	7	0.02239336164118555
1202	8	0.18676757434979407
1202	9	0.2468228097671954
1202	10	0.3420940115567461
1202	11	0.21785378143677292
1202	12	0.11164587717331642
1202	13	0.19139793531470417
1202	14	0.4099166805784904
1202	15	0.3212551910611836
1202	16	0.47514530519739395
1202	17	0.2563727243061962
1202	18	0.18365521632060933
1202	19	0.3003754590977863
1202	20	0.39501339966489724
1202	21	0.468406389683409
1202	22	0.47283562203730156
1202	23	0.7937491481426449
1202	24	0.3365302102486396
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2211	5	0.31905556190145745
2211	6	0.20525141206147168
2211	7	0.16223169688837658
2211	8	0.2580989779652043
2211	9	0.36128863134430134
2211	10	0.4171005731363877
2211	11	0.19196606779637904
2211	12	0.24142531831527236
2211	13	0.0775246011853028
2211	14	0.4843253677807269
2211	15	0.2347382570980585
2211	16	0.18492469247004806
2211	17	0.2640967584820757
2211	18	0.22774955522612572
2211	19	0.3211605532239119
2211	20	0.2962735222295194
2211	21	0.3264298174406406
2211	22	0.38451863055374247
2211	23	0.5565698911738224
2211	24	0.5077533964320082
2211	25	0.538410390636173
2212	1	-0.20381880530044327
2212	2	-0.1900966173575469
2212	3	-0.11790097352037776
2212	4	-0.469885162874526
2212	5	-0.18982338386217634
2212	6	-0.4189155934996762
2212	7	-0.4622022096948015
2212	8	-0.17608634288595226
2212	9	-0.04765177275114496
2212	10	-0.479188030762586
2212	11	-0.3707445659169579
2212	12	-0.5573074840547605
2212	13	-0.479853238658869
2212	14	-0.29801137238050046
2212	15	-0.4759426491064289
2212	16	-0.18599939841971747
2212	17	-0.30086545148451904
2212	18	-0.17932530872353425
2212	19	-0.3318006723025775
2212	20	-0.25768218150104616
2212	21	-0.2596050832979273
2212	22	-0.35568944540617764
2212	23	0.12342796308698922
2212	24	-0.06951904191193847
2212	25	-0.7718313221543411
2213	1	-0.15314754501578065
2213	2	-1.319268252640036
2213	3	-0.3501143250982146
2213	4	-0.029755000657978314
2213	5	-0.05352816926663451
2213	6	-0.07129318393308637
2213	7	-0.1335246810802886
2213	8	0.02687645029494945
2213	9	0.0652189327344459
2213	10	-0.4480707211587571
2213	11	-0.1647612911188432
2213	12	-0.07880221228344908
2213	13	-0.34933207865844196
2213	14	-0.029838346307059282
2213	15	-0.07301528281727343
2213	16	-0.1327864904635021
2213	17	0.04986223281137114
2213	18	0.020695550458675882
2213	19	-0.040422227500975794
2213	20	0.09991420466479894
2213	21	-0.28961697885580406
2213	22	-0.7647460931056997
2213	23	-0.09805678001517038
2213	24	0.10881160126125167
2213	25	-0.43269102085793776
2214	1	-0.20418112531637078
2214	2	-0.4618766768271527
2214	3	-0.39382967827150495
2214	4	-0.5190707808630179
2214	5	-0.2813266048878873
2214	6	-0.2697194944141543
2214	7	-0.5342831932774033
2214	8	-0.08601374912415594
2214	9	-0.08969804127206515
2214	10	-1.987488372197646
2214	11	-0.8934720563128877
2214	12	-0.5283468690360351
2214	13	-0.30003194561125923
2214	14	-0.844150945681676
2214	15	-0.552219392702959
2214	16	-0.771907165692717
2214	17	-0.5749906274503189
2214	18	-0.6007601407842245
2214	19	-1.3792794625630762
2214	20	-2.6264547531266373
2214	21	-2.0207738237079553
2214	22	-2.1964424994835383
2214	23	-2.5797552086428084
2214	24	-1.7409558821415558
2214	25	-1.9913794486176997
2215	1	-0.23347970534721796
2215	2	-0.3993306268470427
2215	3	-0.884513421422497
2215	4	-0.32283694851667377
2215	5	-0.1837248400454925
2215	6	-0.22765867758347724
2215	7	-0.22078144776042308
2215	8	-0.2061414330611413
2215	9	-0.16524999636987303
2215	10	-0.24521419347722428
2215	11	-0.45922896514993994
2215	12	-0.4685367999425978
2215	13	-0.3713765659160444
2215	14	-0.17624935001637
2215	15	-0.6032041632882184
2215	16	-0.4928298718423392
2215	17	-0.30944300970651284
2215	18	-0.3464404606995757
2215	19	-2.5018881593490008
2215	20	-2.567008665393871
2215	21	-3.4644436337248976
2215	22	-2.511749447681275
2215	23	-3.5732857568559595
2215	24	-2.5629235805885386
2215	25	-1.9035914180342814
2216	1	-1.3982691382745998
2216	2	-1.1821260191752998
2216	3	-0.775889757657449
2216	4	-0.8214058138966038
2216	5	-1.8954680837043014
2216	6	-1.4754173955136665
2216	7	-1.1172410985637313
2216	8	-5.359076860542846
2216	9	-4.239613147079879
2216	10	-2.130072130001942
2216	11	-1.0954249860139598
2216	12	-1.0669124228372482
2216	13	-1.9176676185186352
2216	14	-3.4362277677735094
2216	15	-1.928332224100231
2216	16	-2.9602421723884333
2216	17	-2.5836923389117885
2216	18	-2.5337335763266253
2216	19	-1.5174187062941016
2216	20	-0.8572470731879562
2216	21	-0.7163066726971081
2216	22	-0.6865044979005432
2216	23	-0.9877662551050648
2216	24	-0.5608861957936924
2216	25	-0.3269981590342752
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	57.0
8	281.0
9	730.0
10	1540.0
11	2919.0
12	5142.0
13	7513.0
14	9896.0
15	12960.0
16	14791.0
17	15874.0
18	16726.0
19	16981.0
20	17644.0
21	19234.0
22	20256.0
23	21995.0
24	23396.0
25	25357.0
26	27576.0
27	31277.0
28	35825.0
29	42705.0
30	51479.0
31	63314.0
32	85455.0
33	121363.0
34	176445.0
35	281622.0
36	491603.0
37	1634322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17305960192109	20.404399080369927	13.046320411085633	23.376220906623352
2	15.502623180822726	20.114451641342527	40.59583722297556	23.78708795485919
3	18.87606476741041	25.2320950250916	28.885229506184825	27.006610701313168
4	12.294929322397453	16.63715533563847	37.271742574898234	33.79617276706584
5	13.43895611791237	38.1007681296268	34.622036323516255	13.838239428944574
6	32.59198505053107	37.677142629520496	16.488278909423787	13.24259341052464
7	27.26945859583912	32.37302584610519	22.35720606041553	18.000309497640156
8	24.498027025839125	37.84475159571611	20.17173787768537	17.4854835007594
9	24.083560929691956	17.14768144730529	21.538622603523276	37.230135019479484
10	14.968658041638088	30.380410215369324	32.917037895734765	21.733893847257818
11	33.658206493820835	25.3662339263787	21.97281467578969	19.002744904010772
12	21.04083695739761	27.180878858987477	31.146176968288113	20.6321072153268
13	26.970013207338116	23.37448557158594	26.405519306002763	23.249981915073185
14	20.449725663133375	22.57840027543568	27.65396805629827	29.317906005132677
15	23.4000658687332	30.371651660011118	23.249709344294768	22.978573126960917
16	22.34503030182788	30.8030785619337	25.17168492203632	21.6802062142021
17	20.669867733919702	30.08492463369899	27.95959586093519	21.285611771446117
18	21.00509764457138	29.663645806339563	28.64787121984214	20.683385329246914
19	21.59257985226488	28.319408449062028	28.859321380509357	21.22869031816374
20	22.642800021976278	28.034191425588933	28.097678450428234	21.225330102006556
21	22.889572652914477	28.32026304109498	27.62288890063228	21.167275405358264
22	21.607226204061885	28.13661511317901	28.71246814053111	21.543690542228
23	21.666757286939013	28.476687476746736	28.415289696719405	21.44126553959485
24	21.62734422262553	27.793608904825827	28.38880230605394	22.1902445664947
25	22.198909092355173	27.73527609642153	27.635827362381583	22.429987448841718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2227.0
1	2227.0
2	2602.0
3	2977.0
4	2977.0
5	2977.0
6	4253.5
7	5530.0
8	5530.0
9	5530.0
10	8600.0
11	11670.0
12	11670.0
13	11670.0
14	19532.0
15	27394.0
16	27394.0
17	27394.0
18	42424.0
19	57454.0
20	57454.0
21	57454.0
22	81545.5
23	105637.0
24	105637.0
25	105637.0
26	139044.0
27	172451.0
28	172451.0
29	172451.0
30	208351.5
31	244252.0
32	244252.0
33	244252.0
34	279590.5
35	314929.0
36	314929.0
37	314929.0
38	342960.0
39	370991.0
40	370991.0
41	370991.0
42	375799.5
43	380608.0
44	380608.0
45	380608.0
46	388681.5
47	396755.0
48	396755.0
49	396755.0
50	378021.5
51	359288.0
52	359288.0
53	359288.0
54	328335.5
55	297383.0
56	297383.0
57	297383.0
58	261737.0
59	226091.0
60	226091.0
61	226091.0
62	189694.0
63	153297.0
64	153297.0
65	153297.0
66	118627.0
67	83957.0
68	83957.0
69	83957.0
70	61808.0
71	39659.0
72	39659.0
73	39659.0
74	27828.5
75	15998.0
76	15998.0
77	15998.0
78	10746.0
79	5494.0
80	5494.0
81	5494.0
82	3577.5
83	1661.0
84	1661.0
85	1661.0
86	1041.5
87	422.0
88	422.0
89	422.0
90	272.5
91	123.0
92	123.0
93	123.0
94	72.5
95	22.0
96	22.0
97	22.0
98	16.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03204854285958465
2	0.014833896980722037
3	0.020816291647844508
4	0.021426740083265165
5	0.020175320790652814
6	0.005402468653472841
7	9.1567265313099E-5
8	2.44179374168264E-4
9	3.66269061252396E-4
10	3.0522421771033E-5
11	8.241053878178911E-4
12	9.76717496673056E-4
13	0.002167091945743343
14	4.88358748336528E-4
15	0.002045002258659211
16	0.0022891816328274753
17	0.0024723161634536733
18	0.001861867728033013
19	3.35746639481363E-4
20	6.1044843542066E-4
21	0.07847314637332585
22	0.07716068223717142
23	0.07771008582905002
24	0.001037762340215122
25	0.22156225963592857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3276280.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.17859811629384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.44186331561345	57.85654393584865
2	11.650493998260774	16.352306723204727
3	3.0593290674684295	6.440982753940889
4	1.1140883982646292	3.127406478713558
5	0.5491841524301481	1.9270486962616413
6	0.2947676193484101	1.2411826977569251
7	0.18600659676852405	0.9137577541118439
8	0.12436999609996013	0.6982489579219309
9	0.08701560859401927	0.5495970082827963
>10	0.4520544495112144	5.630217263248776
>50	0.02621804265208429	1.2628768036705946
>100	0.012467054557867912	1.5302540372727202
>500	0.00118012064843849	0.5506864163081325
>1k	8.741634381040373E-4	1.4362078351001832
>5k	8.741634381040372E-5	0.48268263835668024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8309	0.2536108024955132	No Hit
CCCATGTACTCTGCGTTGATACCAC	7427	0.22669002649346207	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4616	0.1408914988950883	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3877	0.11833542920629495	No Hit
ATTCCATTCCATTCCATTCCATTCC	3620	0.11049116681113946	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3454	0.10542444479714798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.1044843542066E-5	0.0
5	0.0	0.0	0.0	6.1044843542066E-5	0.0
6	0.0	3.0522421771033E-5	0.0	6.1044843542066E-5	0.0
7	0.0	3.0522421771033E-5	0.0	6.1044843542066E-5	0.0
8	0.0	3.0522421771033E-5	0.0	6.1044843542066E-5	0.0
9	0.0	3.0522421771033E-5	0.0	6.1044843542066E-5	0.0
10	0.0	3.0522421771033E-5	0.0	9.1567265313099E-5	0.0
11	0.0	3.0522421771033E-5	0.0	1.22089687084132E-4	0.0
12	0.0	3.0522421771033E-5	0.0	1.52612108855165E-4	0.0
13	0.0	3.0522421771033E-5	0.0	1.83134530626198E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCGT	40	0.005294707	14.244333	6
CGACTAA	50	0.0014943159	13.306898	17
TAGGCCG	50	0.0015027655	13.296944	5
TGGACCG	80	2.8753871E-5	11.872272	5
TAGACAG	470	0.0	11.518629	5
CCGGAAC	70	0.001493888	10.855642	3
GAACCGC	90	9.560006E-5	10.551358	6
TTAAGCG	75	0.0026569914	10.129613	12
GTCTTAC	310	0.0	10.113204	1
GTCGTAC	85	6.5952935E-4	10.059123	1
CTAGACA	390	0.0	9.498543	4
CGGGACT	80	0.0045234277	9.498542	4
TACATCG	80	0.0045262766	9.497817	5
TGCACCG	80	0.0045262766	9.497817	5
CGCGCGC	80	0.004531979	9.4963665	10
GTCTAGC	205	4.2382453E-10	9.268568	1
TAAGACT	325	0.0	9.060148	4
ACGGTAC	105	4.5010433E-4	9.046369	3
CGCTCAG	105	4.5120443E-4	9.04416	10
TGGACTG	495	0.0	9.018129	5
>>END_MODULE