FastQCFastQC Report
Wed 25 May 2016
SRR1294742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276280
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT127730.3898628932814045No Hit
GGTATCAACGCAGAGTACTTTTTTT105140.320912742500641No Hit
TATCAACGCAGAGTACTTTTTTTTT88440.26994029814301584No Hit
GTACATGGGAAGCAGTGGTATCAAC85540.2610887958294163No Hit
CCCATGTACTCTGCGTTGATACCAC80650.24616333158338113No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49700.15169643620203402No Hit
GAGTACATGGGAAGCAGTGGTATCA44340.13533641813276032No Hit
ATTCCATTCCATTCCATTCCATTCC40900.12483670504352497No Hit
GAATGGAATGGAATGGAATGGAATG34420.10505817573589558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC508.563856E-515.2319541
CGCCCTA907.52308E-611.6033816
ACACCGT1251.8491846E-811.3938016
AGACCGT1001.9442195E-611.39386
CGGGACT700.001471501410.8753074
TAGACTG5050.010.3638465
ACCGCCC1106.097838E-610.3568948
ACCGTCT1054.1374635E-59.9459068
GAGACCG1252.5178706E-69.8965295
GTATTAG4350.09.8482471
TATTCGA1356.5081804E-79.84529216
GTGTAAG6050.09.7560031
ATAGGAC2750.09.68891053
ACTGTTC4750.09.5937558
GTTCTAG3700.09.5199711
GTCCTAG3900.09.5199711
TACTCCG900.00109730019.5158945
CTAAACG800.0044558329.5158934
CTACACG800.0044558329.5158934
ACTCCGC800.0045420219.4938198
Produced by FastQC (version 0.11.5)