FastQCFastQC Report
Wed 25 May 2016
SRR1294741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260395
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1339824.109379385013166No Hit
CCCATGTACTCTGCGTTGATACCAC1287933.950226889686679No Hit
GAGTACATGGGAAGCAGTGGTATCA631271.9361764448786112No Hit
CATGTACTCTGCGTTGATACCACTG487061.4938680742670751No Hit
GCGTTGATACCACTGCTTCCCATGT405681.2442664155723462No Hit
GCTTCCCATGTACTCTGCGTTGATA390131.1965728078959756No Hit
ACGCAGAGTACATGGGAAGCAGTGG324670.9957995887001421No Hit
GTATCAACGCAGAGTACATGGGAAG324280.9946034146169406No Hit
ACTCTGCGTTGATACCACTGCTTCC260880.8001484482708383No Hit
TATCAACGCAGAGTACATGGGAAGC260560.7991669721000063No Hit
GGTATCAACGCAGAGTACATGGGAA218160.6691213794647581No Hit
CAGTGGTATCAACGCAGAGTACATG171510.5260405564356466No Hit
GTGGTATCAACGCAGAGTACATGGG162450.49825251234896384No Hit
GCAGTGGTATCAACGCAGAGTACAT142030.43562206419774285No Hit
GTACATGGTAAGCAGTGGTATCAAC139440.4276782414400709No Hit
ATACCACTGCTTCCCATGTACTCTG132210.40550301420533397No Hit
GTACTCTGCGTTGATACCACTGCTT128230.39329590433061024No Hit
ACATGGGAAGCAGTGGTATCAACGC124450.3817022170626565No Hit
CATGGGAAGCAGTGGTATCAACGCA123900.3800153048940389No Hit
CTGCTTCCCATGTACTCTGCGTTGA123290.3781443659433903No Hit
GGGAAGCAGTGGTATCAACGCAGAG119950.36790020841033066No Hit
TACCACTGCTTCCCATGTACTCTGC103100.3162193537899549No Hit
CTTCCCATGTACTCTGCGTTGATAC102860.31548324666183086No Hit
ACCATGTACTCTGCGTTGATACCAC99630.30557647156249473No Hit
GTTGATACCACTGCTTCCCATGTAC99550.30533110251978673No Hit
GATACCACTGCTTCCCATGTACTCT99440.3049937200860632No Hit
AAGCAGTGGTATCAACGCAGAGTAC98410.3018345936611975No Hit
CCATGTACTCTGCGTTGATACCACT97710.29968761453750237No Hit
CCACTGCTTCCCATGTACTCTGCGT96810.29692721280703716No Hit
GCAGAGTACATGGGAAGCAGTGGTA96800.2968965416766987No Hit
ATGGGAAGCAGTGGTATCAACGCAG84900.2603978965738814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA84380.25880299779627924No Hit
GTACATGGGTAAGCAGTGGTATCAA83020.25463172407024304No Hit
TTCCCATGTACTCTGCGTTGATACC77040.23629038812781888No Hit
TGATACCACTGCTTCCCATGTACTC67640.20745952560962708No Hit
GGTAAGCAGTGGTATCAACGCAGAG62250.19092778635717453No Hit
CCCCATGTACTCTGCGTTGATACCA62090.1904370482717585No Hit
GCTTACCATGTACTCTGCGTTGATA58640.17985550830497532No Hit
ATCAACGCAGAGTACATGGGAAGCA47250.14492109084942162No Hit
GAGTACATGGTAAGCAGTGGTATCA44780.13734532165581165No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA44250.13571975174787104No Hit
AAAAAGTACTCTGCGTTGATACCAC41750.12805196916324557No Hit
GGAAGCAGTGGTATCAACGCAGAGT41020.1258129766485349No Hit
ACTCTGCGTTGATACCACTGCTTAC33560.10293231341601249No Hit
TCCATGTACTCTGCGTTGATACCAC33220.1018894949845034No Hit
GCGTTGATACCACTGCTTACCATGT32880.10084667655299434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA551.9570027E-413.8216661
GGTATCG1351.8189894E-1213.37702517
TATCGAC1301.4551915E-1113.1824519
GTATCGA1501.4551915E-1112.03913818
CCGTGCA907.504008E-611.6060229
GTACAAA7650.011.3035011
CGCAAAA3050.011.2131872
TGATACG700.00148598210.86254717
AAAAGTA8750.010.748642
GAACAAA17500.010.696981
GATACGA803.756292E-410.69265518
GTAGCCC1355.696529E-810.55533
AGCGGTG2400.010.28747211
CGGGTTC1502.4296241E-810.13822118
AAGCGGT2350.010.10210810
GTCTGCG2450.010.0772149
GTTAGGC856.5985485E-410.0585793
ACAGCGT951.6523726E-49.9956188
GTATAGA3050.09.9697251
ACGCAAA4300.09.94436551
Produced by FastQC (version 0.11.5)