FastQCFastQC Report
Wed 25 May 2016
SRR1294741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260395
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1399774.293252811392485No Hit
GTACATGGGAAGCAGTGGTATCAAC1338354.104870728853405No Hit
GAGTACATGGGAAGCAGTGGTATCA679542.08422599102256No Hit
CATGTACTCTGCGTTGATACCACTG553991.6991499496226685No Hit
GCGTTGATACCACTGCTTCCCATGT432961.3279372591357796No Hit
GCTTCCCATGTACTCTGCGTTGATA422871.2969900886242312No Hit
ACGCAGAGTACATGGGAAGCAGTGG401021.2299736688346044No Hit
GTATCAACGCAGAGTACATGGGAAG345971.0611290963211513No Hit
TATCAACGCAGAGTACATGGGAAGC301310.9241518282294017No Hit
ACTCTGCGTTGATACCACTGCTTCC299390.9182629712044094No Hit
GGTATCAACGCAGAGTACATGGGAA243610.7471794061762455No Hit
CAGTGGTATCAACGCAGAGTACATG207680.6369780348700081No Hit
GTGGTATCAACGCAGAGTACATGGG176880.5425109534274222No Hit
GCAGTGGTATCAACGCAGAGTACAT164800.5054602279785118No Hit
CATGGGAAGCAGTGGTATCAACGCA161670.49586016418256074No Hit
ACATGGGAAGCAGTGGTATCAACGC159670.4897259381148603No Hit
ATACCACTGCTTCCCATGTACTCTG148240.4546688361379526No Hit
GGGAAGCAGTGGTATCAACGCAGAG142620.4374316608877145No Hit
GTACATGGTAAGCAGTGGTATCAAC138100.4235683099747116No Hit
CTGCTTCCCATGTACTCTGCGTTGA130780.40111704256692826No Hit
GTACTCTGCGTTGATACCACTGCTT123310.3782057082040673No Hit
AAGCAGTGGTATCAACGCAGAGTAC119600.36682671884848306No Hit
CTTCCCATGTACTCTGCGTTGATAC118970.36489443763715745No Hit
GATACCACTGCTTCCCATGTACTCT114220.35032565072636906No Hit
CCATGTACTCTGCGTTGATACCACT113800.34903746325215196No Hit
TACCACTGCTTCCCATGTACTCTGC113490.3480866582116584No Hit
GCAGAGTACATGGGAAGCAGTGGTA111030.34054156014838693No Hit
ACCATGTACTCTGCGTTGATACCAC108610.33311914660646946No Hit
CCACTGCTTCCCATGTACTCTGCGT106560.32683156488707654No Hit
ATGGGAAGCAGTGGTATCAACGCAG104440.3203292852553142No Hit
GTTGATACCACTGCTTCCCATGTAC101890.31250814701899615No Hit
GTACATGGGTAAGCAGTGGTATCAA89550.2746599721812848No Hit
TTCCCATGTACTCTGCGTTGATACC79960.2452463581866614No Hit
GTATCAACGCAGAGTACTTTTTTTT79060.24248595645619625No Hit
GGTAAGCAGTGGTATCAACGCAGAG75440.23138300727365857No Hit
CCCCATGTACTCTGCGTTGATACCA70960.2176423408820097No Hit
TGATACCACTGCTTCCCATGTACTC70720.21690623375388565No Hit
TATCAACGCAGAGTACTTTTTTTTT65480.20083456145651063No Hit
GCTTACCATGTACTCTGCGTTGATA63700.19537510025625732No Hit
GGTATCAACGCAGAGTACTTTTTTT60940.18690986828283077No Hit
ATCAACGCAGAGTACATGGGAAGCA57980.1778312137026342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57410.17608295927333958No Hit
GAGTACATGGTAAGCAGTGGTATCA53190.16313974227049177No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52690.16160618575356667No Hit
GGAAGCAGTGGTATCAACGCAGAGT50420.15464383916672672No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45840.14059646147169283No Hit
AAAAAGTACTCTGCGTTGATACCAC45140.13844948234799773No Hit
ATGGTAAGCAGTGGTATCAACGCAG38580.11832922084594046No Hit
TGGGAAGCAGTGGTATCAACGCAGA36870.1130844575580566No Hit
TCCATGTACTCTGCGTTGATACCAC35840.10992533113319092No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35750.1096492909601444No Hit
ACTCTGCGTTGATACCACTGCTTAC35610.10921989513540536No Hit
GCGTTGATACCACTGCTTACCATGT33960.10415915862955255No Hit
CTGCGTTGATACCACTGCTTCCCAT33780.10360707828345951No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA33700.10336170924075151No Hit
ACGCAGAGTACATGGTAAGCAGTGG32900.10090801881367135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT604.1135369E-412.6595816
AAAAAGT9500.011.7245641
TATACCG657.9191575E-411.7112735
AGTACTC12750.011.3438615
GGTTAGA1354.5820343E-911.2828981
TACACCG600.005807516611.1013115
AAAAGTA10450.010.9274582
TCTGTCG1252.2614404E-710.6335598
TCTAGAC2001.8189894E-1210.466953
ATTAGAC1105.9347403E-610.3804463
CTAGACT1303.760888E-710.2473644
ACACCGT750.002661227710.1276656
AAGTACT14150.010.0870074
TAACGCA856.4995076E-410.0751395
TTAGACT1909.640644E-1110.016224
AAAGTAC11750.09.7988473
GTGCTAA2251.8189894E-129.73151
CAAGTAC3450.09.6533143
ATTAACG1002.7124071E-49.5154093
TTAACGC1002.7124071E-49.5154094
Produced by FastQC (version 0.11.5)