FastQCFastQC Report
Wed 25 May 2016
SRR1294740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566400
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC59440.2316084788029925No Hit
CCCATGTACTCTGCGTTGATACCAC51050.1989167705735661No Hit
GTATCAACGCAGAGTACTTTTTTTT38000.14806733167082295No Hit
GGTATCAACGCAGAGTACTTTTTTT29240.11393391521197008No Hit
GAGTACATGGGAAGCAGTGGTATCA26940.10497194513715712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG550.00306704812.0921361
ATACCGT802.8794077E-511.870426
AGACCGT1002.4102794E-510.4459716
CCTAGAC2800.010.17742353
TATACCG750.002653199510.1312035
TAGACAG4750.09.9978975
CGCCCTA800.00449379869.50596816
CTAGACA2800.09.4983734
TGTATCG800.0045249849.4980025
GTCGCCC1502.66622E-79.49596711
AGTGCGT800.0045329929.4959678
CCAGGAC5050.09.4048793
TTACACT4550.09.3939954
TAAGACT2950.09.3373834
TCTATAC3900.09.2553653
GTGTAGG3700.09.2441821
CTAGGAC3550.09.0975663
GTATTAG3700.08.9873991
GTCTAGA2750.08.9827311
TCGCCCA1804.2500687E-88.96841312
Produced by FastQC (version 0.11.5)