FastQCFastQC Report
Wed 25 May 2016
SRR1294740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566400
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102540.3995480049875311No Hit
GGTATCAACGCAGAGTACTTTTTTT81950.3193188902743142No Hit
TATCAACGCAGAGTACTTTTTTTTT67130.2615726309226933No Hit
GTACATGGGAAGCAGTGGTATCAAC62180.2422849127182045No Hit
CCCATGTACTCTGCGTTGATACCAC55030.21442487531172072No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38410.14966490024937656No Hit
GAGTACATGGGAAGCAGTGGTATCA31640.123285536159601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT400.00523240114.2696034
TACACCG604.0464394E-412.6840925
ACACCGT658.054844E-411.6874036
ACTATCG1157.135168E-811.5587911
ATCGGTC1157.135168E-811.5587914
TATCGGT1157.135168E-811.5587913
GTATTAG3150.011.4805651
CTATCGG1201.2862438E-711.07717312
TAGTCCG700.001474962310.8720785
AACACCG700.001474962310.8720785
TCCGTGC700.001498667310.8513198
ACACCGC803.7871292E-410.6830186
CCGTGCA1201.5352089E-610.2861479
TCGGTCA1303.873556E-710.22508315
CCTATAC3100.010.1268163
CACACCG856.513219E-410.072665
ACTGATC2950.09.6558358
CTAGGAC3300.09.5130693
AGCGTGG900.0011197129.4947210
CGGTCAG1502.6713133E-79.4947216
Produced by FastQC (version 0.11.5)