FastQCFastQC Report
Wed 25 May 2016
SRR1294739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265840
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC736133.2488172156904285No Hit
CCCATGTACTCTGCGTTGATACCAC700373.090994951099813No Hit
GAGTACATGGGAAGCAGTGGTATCA341131.5055343713589662No Hit
CATGTACTCTGCGTTGATACCACTG267151.1790329414256964No Hit
GCTTCCCATGTACTCTGCGTTGATA225510.9952600360131342No Hit
GCGTTGATACCACTGCTTCCCATGT215090.9492726759170992No Hit
GTATCAACGCAGAGTACATGGGAAG184170.812811142887406No Hit
ACGCAGAGTACATGGGAAGCAGTGG170490.7524361826077747No Hit
TATCAACGCAGAGTACATGGGAAGC137500.6068389647989267No Hit
ACTCTGCGTTGATACCACTGCTTCC135600.5984535536489779No Hit
GGTATCAACGCAGAGTACATGGGAA113330.5001677082229989No Hit
CAGTGGTATCAACGCAGAGTACATG94540.41724040532429474No Hit
GTGGTATCAACGCAGAGTACATGGG88250.3894802810436747No Hit
GTACATGGTAAGCAGTGGTATCAAC86860.3833456907813438No Hit
GCAGTGGTATCAACGCAGAGTACAT79660.3515693958973273No Hit
ATACCACTGCTTCCCATGTACTCTG69910.30853899657522155No Hit
GTACTCTGCGTTGATACCACTGCTT69800.3080535254033824No Hit
CTGCTTCCCATGTACTCTGCGTTGA66790.2947692687921477No Hit
CATGGGAAGCAGTGGTATCAACGCA66320.29269498287610773No Hit
ACATGGGAAGCAGTGGTATCAACGC66230.29229777919005756No Hit
GGGAAGCAGTGGTATCAACGCAGAG65810.29044416198848994No Hit
ACCATGTACTCTGCGTTGATACCAC60050.2650231260812767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA59780.2638315150231261No Hit
CCATGTACTCTGCGTTGATACCACT55590.24533947675034426No Hit
TACCACTGCTTCCCATGTACTCTGC55460.24476573809271615No Hit
GCAGAGTACATGGGAAGCAGTGGTA53780.23735126928644562No Hit
CTTCCCATGTACTCTGCGTTGATAC53630.23668926314302863No Hit
AAGCAGTGGTATCAACGCAGAGTAC53110.23439430851251633No Hit
GTACATGGGTAAGCAGTGGTATCAA51890.22900999187939133No Hit
GATACCACTGCTTCCCATGTACTCT51740.22834798573597428No Hit
CCACTGCTTCCCATGTACTCTGCGT51430.2269798397062458No Hit
GTTGATACCACTGCTTCCCATGTAC49190.21709388129788512No Hit
ATGGGAAGCAGTGGTATCAACGCAG47100.20786992903294144No Hit
TTCCCATGTACTCTGCGTTGATACC39980.17644670409208066No Hit
GGTAAGCAGTGGTATCAACGCAGAG38410.16951770645764924No Hit
CCCCATGTACTCTGCGTTGATACCA37690.1663400769692476No Hit
GCTTACCATGTACTCTGCGTTGATA36850.16263284256611235No Hit
TGATACCACTGCTTCCCATGTACTC35060.15473290258800268No Hit
GAGTACATGGTAAGCAGTGGTATCA28750.12688451082159374No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA27470.12123539173110193No Hit
ATCAACGCAGAGTACATGGGAAGCA27250.12026444938742366No Hit
GGAAGCAGTGGTATCAACGCAGAGT23330.10296402217279244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGT653.388648E-614.6091898
GCGTTAT400.005275613614.25182151
CTAAGAC802.0041316E-613.0604213
GTCTCGC905.400234E-712.6682861
AAGCGGT1501.8189894E-1212.66129710
TAACCCG550.003075392712.0876515
CGCAAAA1302.6229827E-911.6909632
AGCACCG658.0440746E-411.6891575
CCAATAC1605.456968E-1010.6857993
TATCGAC1105.8877868E-610.38697819
GGTATCG1106.005457E-610.3697717
GGAAGCG1856.002665E-1110.2659178
ACAGTGT3250.010.2264328
GTCTTAC1409.6753865E-810.1798731
ATCTTCG750.002657703410.1290387
GTCTTAG1606.162736E-910.095041
TCTAGAC1606.19184E-910.0921433
TAGGACA1606.200935E-910.0912534
GTCCTAG1901.0004442E-1010.0012781
GTAAGAC1856.511982E-109.7552033
Produced by FastQC (version 0.11.5)