FastQCFastQC Report
Wed 25 May 2016
SRR1294739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265840
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC755363.333686403276489No Hit
GTACATGGGAAGCAGTGGTATCAAC740163.2666031140768985No Hit
GAGTACATGGGAAGCAGTGGTATCA367431.6216061151714156No Hit
CATGTACTCTGCGTTGATACCACTG301061.3286904635808354No Hit
GCTTCCCATGTACTCTGCGTTGATA249661.101843025103273No Hit
GCGTTGATACCACTGCTTCCCATGT231011.0195335946050912No Hit
ACGCAGAGTACATGGGAAGCAGTGG210950.9310013063587896No Hit
GTATCAACGCAGAGTACATGGGAAG201030.887220633407478No Hit
TATCAACGCAGAGTACATGGGAAGC162330.7164230484058892No Hit
ACTCTGCGTTGATACCACTGCTTCC153490.67740881968718No Hit
GGTATCAACGCAGAGTACATGGGAA132280.5838011510080147No Hit
CAGTGGTATCAACGCAGAGTACATG116240.5130106274052889No Hit
GTGGTATCAACGCAGAGTACATGGG98250.43361402393814213No Hit
GCAGTGGTATCAACGCAGAGTACAT92760.40938459908907954No Hit
CATGGGAAGCAGTGGTATCAACGCA87910.38797973378526285No Hit
ACATGGGAAGCAGTGGTATCAACGC86440.38149207357977616No Hit
GTACATGGTAAGCAGTGGTATCAAC85220.37610775694665116No Hit
GGGAAGCAGTGGTATCAACGCAGAG79620.3513928609257494No Hit
ATACCACTGCTTCCCATGTACTCTG77020.3399180877731879No Hit
CTGCTTCCCATGTACTCTGCGTTGA72600.3204109734138333No Hit
ACCATGTACTCTGCGTTGATACCAC68390.3018306676552625No Hit
GTACTCTGCGTTGATACCACTGCTT68240.30116866151184546No Hit
CCATGTACTCTGCGTTGATACCACT66370.2929156515905801No Hit
AAGCAGTGGTATCAACGCAGAGTAC65680.2898704233308618No Hit
GTATCAACGCAGAGTACTTTTTTTT63280.27927832503618966No Hit
CTTCCCATGTACTCTGCGTTGATAC62900.2776012428061999No Hit
GCAGAGTACATGGGAAGCAGTGGTA61390.27093704762913534No Hit
GATACCACTGCTTCCCATGTACTCT60450.2667884757970554No Hit
TACCACTGCTTCCCATGTACTCTGC59580.26294884016523673No Hit
CCACTGCTTCCCATGTACTCTGCGT58540.25835893090421214No Hit
ATGGGAAGCAGTGGTATCAACGCAG57600.2542103590721322No Hit
GTACATGGGTAAGCAGTGGTATCAA56120.247678565123751No Hit
GTTGATACCACTGCTTCCCATGTAC52410.23130494650990363No Hit
GGTAAGCAGTGGTATCAACGCAGAG48790.2153285315821064No Hit
GGTATCAACGCAGAGTACTTTTTTT45740.20186773999929386No Hit
TATCAACGCAGAGTACTTTTTTTTT44890.1981163718532641No Hit
CCCCATGTACTCTGCGTTGATACCA43200.19065776930409914No Hit
GCTTACCATGTACTCTGCGTTGATA42850.18911308830279278No Hit
TTCCCATGTACTCTGCGTTGATACC41320.18236062563993927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39560.174593086890513No Hit
TGATACCACTGCTTCCCATGTACTC37830.16695794936977015No Hit
ATCAACGCAGAGTACATGGGAAGCA33580.14820110863962152No Hit
GAGTACATGGTAAGCAGTGGTATCA33380.14731843378173215No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32990.14559721780884793No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30480.13451964834233662No Hit
GGAAGCAGTGGTATCAACGCAGAGT28900.12754651696501076No Hit
AAAAAGTACTCTGCGTTGATACCAC25730.11355612046746462No Hit
ATGGTAAGCAGTGGTATCAACGCAG24860.10971648483564594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGG500.001506799813.2919236
TTTAGAC1201.2485543E-711.1008523
TCGTGTG600.00590370511.0766019
TTACACT2850.010.6835274
GTTCTAC1355.5226337E-810.5771531
CTTACAC2800.010.5344823
GTAGGAC1002.3582337E-510.4665183
GTCTTAG1855.820766E-1110.2912841
ATTAGAC1606.017217E-910.1097053
TGAACTG1803.8380676E-1010.0436295
GTCTAGC1054.0152554E-59.9727441
TGCACTG3150.09.9681135
TCTAGAC1252.519555E-69.89561753
CTAGCAC1553.967034E-89.8219533
GTCTTGC1659.889845E-99.8079041
GTTCTAG1606.431583E-89.5194381
GTATTGG2002.5465852E-109.5194381
GTGCGGG800.00444127069.5194381
TCCTATA1304.154337E-69.5152272
TACGGAA900.00109772799.5152262
Produced by FastQC (version 0.11.5)