FastQCFastQC Report
Wed 25 May 2016
SRR1294738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794480
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC63230.1318808296207305No Hit
CCCATGTACTCTGCGTTGATACCAC58850.12274532378902403No Hit
GTATCAACGCAGAGTACTTTTTTTT57690.12032587475596937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT759.727592E-713.9278887
ACCGTTC1355.730908E-810.551328
AGACCGT1659.367795E-1010.359916
TCGGACG856.623947E-410.0545779
GAGTCGT1054.1253305E-59.9488036
ACGGGTC1151.0292879E-59.9116515
CGAATTA1356.427472E-79.8545815
ACCGTCT1856.548362E-109.7528418
TAGACTG5750.09.7464565
CACACCG2750.09.6713685
GTCGTAC1002.7542002E-49.50114251
GTCTTAG4800.09.50114251
CCGATCA900.0011187179.495999
CCGTGCG1304.2664597E-69.495999
TCGTCCG900.0011187179.495999
TCGTTTG1002.769536E-49.49589113
CGGACGT900.00111882379.49589110
TCGTCTA1606.691698E-89.49579114
CTACACT7950.09.4393194
GTCTTAC4700.09.298991
Produced by FastQC (version 0.11.5)