##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294738_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4794480
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04278274181976	33.0	33.0	33.0	27.0	33.0
2	31.05645262885652	33.0	33.0	33.0	27.0	33.0
3	30.914122908010878	33.0	33.0	33.0	27.0	33.0
4	34.3021649897382	37.0	37.0	37.0	27.0	37.0
5	34.59981541272463	37.0	37.0	37.0	27.0	37.0
6	34.83885739433682	37.0	37.0	37.0	33.0	37.0
7	34.77870196559377	37.0	37.0	37.0	27.0	37.0
8	34.70574306285562	37.0	37.0	37.0	27.0	37.0
9	34.3883265755619	37.0	37.0	37.0	27.0	37.0
10	34.45146230665265	37.0	37.0	37.0	27.0	37.0
11	34.72843499190736	37.0	37.0	37.0	27.0	37.0
12	34.633454097211796	37.0	37.0	37.0	27.0	37.0
13	34.58179239458711	37.0	37.0	37.0	27.0	37.0
14	35.67683794697235	37.0	37.0	40.0	27.0	40.0
15	36.01231541272463	40.0	37.0	40.0	27.0	40.0
16	36.03781265121557	40.0	37.0	40.0	27.0	40.0
17	35.97579236955833	40.0	37.0	40.0	27.0	40.0
18	35.97464751130467	37.0	37.0	40.0	27.0	40.0
19	36.0457563698253	40.0	37.0	40.0	27.0	40.0
20	35.92259160534615	40.0	37.0	40.0	27.0	40.0
21	35.86497284377034	40.0	37.0	40.0	27.0	40.0
22	35.72543487510637	37.0	37.0	40.0	27.0	40.0
23	35.323065066493136	37.0	33.0	40.0	27.0	40.0
24	35.62728929936093	37.0	37.0	40.0	27.0	40.0
25	34.42261141979944	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5525318013619511
1101	2	0.7020997391628008
1101	3	0.47197075909324937
1101	4	0.5739025187859923
1101	5	0.5677618436947114
1101	6	0.4861276224792732
1101	7	0.4995416433792812
1101	8	0.6702878792857732
1101	9	0.8522360099017874
1101	10	0.8601406972188244
1101	11	0.5795323503943308
1101	12	0.6239536681720139
1101	13	0.6740702360482089
1101	14	1.2801287412214322
1101	15	1.0783031755367602
1101	16	1.1209039961720322
1101	17	1.0322589976222218
1101	18	1.0186168760893963
1101	19	1.0103190461036462
1101	20	1.0935773958017592
1101	21	1.1266828905824866
1101	22	1.1839970274698786
1101	23	1.5713859423891918
1101	24	1.2714754249757831
1101	25	1.1780373630611791
1102	1	0.20681912477659736
1102	2	0.41992618009003735
1102	3	0.2213810951587476
1102	4	0.15529961681357207
1102	5	0.2254973591039331
1102	6	0.190142238712113
1102	7	0.1939016275954515
1102	8	0.3337428664552107
1102	9	0.4049420759745388
1102	10	0.5499787890465129
1102	11	0.3267575851697657
1102	12	0.4443974902117702
1102	13	0.32647881088498565
1102	14	1.0421554491987095
1102	15	0.7684608338342684
1102	16	0.48822133166405024
1102	17	0.7076952291840755
1102	18	0.6866078365577977
1102	19	0.687145013978359
1102	20	0.8468256264796139
1102	21	0.7195039840671811
1102	22	0.8290673420035546
1102	23	1.181294014277313
1102	24	0.8736651552103183
1102	25	0.7906302640359542
1103	1	0.1641724655013519
1103	2	0.38510287078097605
1103	3	0.06474404580745485
1103	4	-0.1632766062559199
1103	5	0.08124169421154903
1103	6	0.0711064933573482
1103	7	0.07048652934719257
1103	8	0.21183401778747424
1103	9	0.430512259847994
1103	10	0.43492300489324265
1103	11	0.3302661980261945
1103	12	0.2968687656321549
1103	13	0.23569250536542796
1103	14	0.841396548515668
1103	15	0.6246874075875795
1103	16	0.35951241794492006
1103	17	0.5054182205931639
1103	18	0.5699005988156003
1103	19	0.5351769188484354
1103	20	0.6237262824145233
1103	21	0.7931994551218935
1103	22	0.5817253013774106
1103	23	1.1363259462521427
1103	24	0.735231445034934
1103	25	0.5691797921551967
1104	1	0.274201964983142
1104	2	0.4807278394359962
1104	3	0.22612429438085258
1104	4	0.08905945495416034
1104	5	0.1340803509045685
1104	6	0.10126218278156784
1104	7	0.09155929415550901
1104	8	0.2989066522323185
1104	9	0.5005174838793565
1104	10	0.5488684316429726
1104	11	0.3205125501825492
1104	12	0.2681088275927692
1104	13	0.35644463773370916
1104	14	0.825343093796647
1104	15	0.6859880221152892
1104	16	0.6008044069671783
1104	17	0.5700980809713485
1104	18	0.49479166067511926
1104	19	0.5505554973771609
1104	20	0.6240883020927583
1104	21	0.6264737900190056
1104	22	0.7748626612861216
1104	23	1.2397986298047812
1104	24	0.9099208311226548
1104	25	0.7585129988165775
1105	1	0.2317356508291013
1105	2	0.33099543767293227
1105	3	0.29048688536006395
1105	4	0.2218180917173953
1105	5	0.154602870109386
1105	6	0.19835278336226736
1105	7	0.1677197345383732
1105	8	0.23433656282232818
1105	9	-1.595986751704487
1105	10	-0.19891630134234362
1105	11	0.0018041258367276214
1105	12	0.14164443446117048
1105	13	0.29469619262467717
1105	14	-1.4336598107486864
1105	15	-0.18144079776068622
1105	16	-0.04036405268917065
1105	17	0.26928577673530185
1105	18	0.15369626013450954
1105	19	0.2878872658117473
1105	20	0.42584467501861667
1105	21	0.5464439661023732
1105	22	0.5285372028099786
1105	23	0.900308087032549
1105	24	0.6545419112560324
1105	25	0.20167689712637582
1106	1	0.12238816251078433
1106	2	0.17157078049288899
1106	3	0.18239091755713588
1106	4	-1.1150417513876718
1106	5	-0.20718314593688092
1106	6	-0.008243305006502055
1106	7	0.06235453666113955
1106	8	0.18242265762879128
1106	9	0.4597456847143775
1106	10	0.42220206260899573
1106	11	0.43989282331561697
1106	12	0.337839519526014
1106	13	0.2269113032829324
1106	14	0.6132277698148556
1106	15	0.3563258847349786
1106	16	0.29229624819051736
1106	17	0.05477359871520093
1106	18	0.2440493597165201
1106	19	0.265465695583849
1106	20	0.33538536125671214
1106	21	0.47523278483503617
1106	22	0.6002480631812048
1106	23	0.8399818725071029
1106	24	0.4637279888128205
1106	25	0.36861718111458686
1107	1	0.2941249493779843
1107	2	0.2757447246895808
1107	3	0.13219392162983112
1107	4	0.5123356827039771
1107	5	0.43848052514215397
1107	6	0.1744501581618607
1107	7	0.2579626459619391
1107	8	0.26562784759450864
1107	9	0.3727151980168557
1107	10	0.43188501546132585
1107	11	0.16314361075601624
1107	12	0.28286850211024017
1107	13	0.28934649324359896
1107	14	0.7173779872959969
1107	15	0.52691358791418
1107	16	0.5372966853581147
1107	17	0.5368358808162625
1107	18	0.5568688422338752
1107	19	0.4750592480059339
1107	20	0.683011247759012
1107	21	0.6818259507982063
1107	22	0.6233088489174108
1107	23	0.8393949629693438
1107	24	0.6824817956898315
1107	25	0.4262562046522689
1108	1	0.49798919062537195
1108	2	0.44039757459526285
1108	3	0.5697130774649963
1108	4	0.7243340031611254
1108	5	0.6324723225894076
1108	6	0.5251631103943808
1108	7	0.4666466910835041
1108	8	0.5940055433712885
1108	9	0.3050255174820151
1108	10	0.6114746396969082
1108	11	0.41206187745027023
1108	12	0.4932300295867478
1108	13	0.45973652323640124
1108	14	-0.8347428997072015
1108	15	0.06835646392591599
1108	16	0.47096367879053247
1108	17	0.5973496127968758
1108	18	0.63597421302957
1108	19	0.6090172355329031
1108	20	0.7460501067838479
1108	21	0.7964026740512224
1108	22	0.8685014351312219
1108	23	1.1685418310706837
1108	24	1.0419446551729123
1108	25	0.9046703506038867
1109	1	0.41250965513609117
1109	2	0.29998074457817125
1109	3	0.4597136297795501
1109	4	0.6209598351084864
1109	5	0.5952772131920909
1109	6	0.398192294734919
1109	7	0.3162913044002238
1109	8	0.34001472967086954
1109	9	0.5815086009775285
1109	10	0.5691164751783617
1109	11	0.4112530426718948
1109	12	0.4347365975796791
1109	13	0.35859204024401947
1109	14	0.7723698742550411
1109	15	0.482244854060383
1109	16	0.4427195294790707
1109	17	0.46096914851985815
1109	18	0.5539395802683984
1109	19	0.43631579791573927
1109	20	0.6757620755515248
1109	21	0.6445650309082822
1109	22	0.7829907208840368
1109	23	1.1533010905829002
1109	24	0.9146763938040223
1109	25	0.6710031329210295
1110	1	0.41474331251316343
1110	2	0.2826657457035715
1110	3	0.36157044559032414
1110	4	0.7054303646920417
1110	5	0.558722556232901
1110	6	0.36043548168355954
1110	7	0.47440898630959083
1110	8	0.40618405187033346
1110	9	0.5091784859494837
1110	10	0.48022154725274646
1110	11	0.2839161110279491
1110	12	0.2993875974150342
1110	13	0.2925737944555564
1110	14	0.7254697905432081
1110	15	0.5221815371012539
1110	16	0.426420354985126
1110	17	0.5618430401680499
1110	18	0.5747695574318996
1110	19	0.46265289065583204
1110	20	0.6185975033121736
1110	21	0.7208257749965838
1110	22	0.7471668133780582
1110	23	0.8986205238223803
1110	24	0.7577155042751968
1110	25	0.6177188072928601
1111	1	0.3925658148217259
1111	2	0.2530948531887738
1111	3	0.48552063850103266
1111	4	0.8370818985946471
1111	5	0.6829360534514635
1111	6	0.4866307881706007
1111	7	0.4979887757818844
1111	8	0.5224200899217308
1111	9	0.514619626069539
1111	10	0.4178031323198823
1111	11	0.39362534370877
1111	12	0.4383008739870675
1111	13	0.39808111200054697
1111	14	-1.440302178832475
1111	15	-0.24329554798364939
1111	16	0.24572085009642564
1111	17	0.41300418323115906
1111	18	0.5033471272116756
1111	19	0.5297386534570947
1111	20	0.5129244321919018
1111	21	0.6563455674615355
1111	22	0.4780985386123646
1111	23	-1.7266410237455858
1111	24	-0.245716132088738
1111	25	0.27382250702839883
1112	1	-0.08828687969293725
1112	2	-0.2028669862237713
1112	3	0.0998485145894712
1112	4	0.2348466351621994
1112	5	-0.7008781995881392
1112	6	-0.9278874895871141
1112	7	-1.0685976372834034
1112	8	-0.7181483062100398
1112	9	-0.7631655751663686
1112	10	-0.9075006275613404
1112	11	-0.9486679029506746
1112	12	-0.8360362312655312
1112	13	-0.9380420371507725
1112	14	-0.6125213915446466
1112	15	-0.9625762750812825
1112	16	-0.9102859313328864
1112	17	-1.0323657858712068
1112	18	-1.0816996205111309
1112	19	-0.9431346261063283
1112	20	-1.0088339242143434
1112	21	-1.8700436223783612
1112	22	-1.7970013771213544
1112	23	-1.45289558816917
1112	24	-1.6294633733711947
1112	25	-1.9805174071002511
1113	1	0.11410390302267004
1113	2	-0.02751598378459974
1113	3	0.031024174454223896
1113	4	0.2835563541619379
1113	5	0.11297737381499928
1113	6	0.007707925567466134
1113	7	0.13632333759116477
1113	8	0.15538839040090124
1113	9	0.3533264826597957
1113	10	0.033252639537629136
1113	11	-0.04405370817406862
1113	12	-0.04580609661544344
1113	13	-0.09484940225279814
1113	14	0.03163501276257108
1113	15	-0.06122451907754822
1113	16	0.06764781447788693
1113	17	0.0927290046180147
1113	18	0.11421526635503199
1113	19	-0.017240263959067192
1113	20	-0.574778160436999
1113	21	-0.4364591339098851
1113	22	0.05634216899481004
1113	23	-0.14849754743701737
1113	24	0.15688630041467633
1113	25	-1.0483831337942817
1114	1	-0.843494484215821
1114	2	-0.7247434192780275
1114	3	-0.520068089841697
1114	4	-1.4737966964678506
1114	5	-0.7202012738907726
1114	6	-0.5636266507836041
1114	7	-0.4937191602843356
1114	8	-0.4690338190960688
1114	9	-0.4680493585606271
1114	10	-0.6069865623385411
1114	11	-0.5354075489289514
1114	12	-0.634375984681931
1114	13	-0.5703013167912445
1114	14	-0.5664360044398435
1114	15	-1.313597133800144
1114	16	-0.8550832401388817
1114	17	-0.9019778949340065
1114	18	-0.810656160479418
1114	19	-0.8536344099198558
1114	20	-2.0820671566606563
1114	21	-6.936024584866733
1114	22	-7.988598995139515
1114	23	-8.031205927123349
1114	24	-5.921534636293249
1114	25	-7.428779857475629
1115	1	-1.1137789982438342
1115	2	-1.1582970323061055
1115	3	-0.8232314327450041
1115	4	-0.6392293455493885
1115	5	-0.6156994175117987
1115	6	-0.9492662502764446
1115	7	-1.1280216248254646
1115	8	-0.8199741056332641
1115	9	-2.754189562040086
1115	10	-1.7141410784263442
1115	11	-1.3237784972089344
1115	12	-1.1001289874168023
1115	13	-1.5586441651587393
1115	14	-1.1418871830145676
1115	15	-2.083112604601709
1115	16	-1.3763919741670705
1115	17	-3.2560858064562055
1115	18	-2.9880432095863725
1115	19	-2.162555121549893
1115	20	-1.8990786718642738
1115	21	-1.3038683056667466
1115	22	-0.8997414045903014
1115	23	-0.40865561249689364
1115	24	-1.4646628741180692
1115	25	-1.1419982740763572
1116	1	-1.5701274081615075
1116	2	-0.7750854945083283
1116	3	-0.5954220581492358
1116	4	-0.2724296619924331
1116	5	-0.21037067762638628
1116	6	-0.36253001070704016
1116	7	-0.7461570355264655
1116	8	-1.7338661360819856
1116	9	-0.7948362033665433
1116	10	-2.327436599275991
1116	11	-1.1129622052751031
1116	12	-0.8195042072190191
1116	13	-0.745097775927249
1116	14	-1.1795814880079902
1116	15	-1.1670167084730423
1116	16	-0.6967459545078611
1116	17	-0.6547393881926666
1116	18	-1.2314634140756198
1116	19	-0.7290238913409013
1116	20	-1.099350112030642
1116	21	-0.5495095581420983
1116	22	-0.14197156658169519
1116	23	0.008734384793363859
1116	24	-0.15767330493569887
1116	25	-0.023221203239735644
1201	1	0.19643978976318976
1201	2	0.3792894511931877
1201	3	0.28730324089189097
1201	4	0.29276676112841926
1201	5	0.2920181792501211
1201	6	0.2852880923459864
1201	7	0.2997254557891864
1201	8	0.3449804054256802
1201	9	0.6452728768292033
1201	10	0.6291174070426635
1201	11	0.47196075362988665
1201	12	0.41579662859338384
1201	13	0.3805598185753354
1201	14	1.0113297906732726
1201	15	0.80496954916795
1201	16	0.7204903832009393
1201	17	0.7233262208528686
1201	18	0.6195152634692889
1201	19	0.5591011641555141
1201	20	0.7380454022040652
1201	21	0.6844232968079993
1201	22	0.8352452039528799
1201	23	0.5989471449160533
1201	24	0.6703799396284325
1201	25	0.624819530511239
1202	1	-0.420826032861946
1202	2	-0.11351909243794367
1202	3	-0.1914720109048993
1202	4	-0.2673627985336111
1202	5	-0.10637642705854944
1202	6	-0.18879663975060623
1202	7	-0.2170579445701506
1202	8	-0.06199118295857886
1202	9	0.005067091522029443
1202	10	0.25066443314644005
1202	11	0.00795911264540905
1202	12	0.0021634833916053253
1202	13	0.06655848283952537
1202	14	0.24196415844648556
1202	15	0.1879670894297476
1202	16	0.2459718947745344
1202	17	0.01747917663227838
1202	18	0.07434381823249225
1202	19	0.051231934462038
1202	20	0.25236781101789774
1202	21	0.30179834185238974
1202	22	0.25621953908995465
1202	23	0.5152313242255033
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2211	5	0.45965698057749194
2211	6	0.3232640569963152
2211	7	0.3511648056758361
2211	8	0.5020715881728108
2211	9	0.6425277860879959
2211	10	0.5802320775489207
2211	11	0.3626253036942657
2211	12	0.47031056678652305
2211	13	0.29170942984468695
2211	14	0.6629031319876262
2211	15	0.47211365801916116
2211	16	0.3529835848440186
2211	17	0.47452292796764084
2211	18	0.4146677105846095
2211	19	0.4960728049310248
2211	20	0.5559503891274105
2211	21	0.5449052325660375
2211	22	0.677182226452274
2211	23	0.7688963806219107
2211	24	0.7044982971933251
2211	25	0.7726541897801624
2212	1	-0.29370473214285653
2212	2	-0.290460180003592
2212	3	-0.26687131030108446
2212	4	-0.5831048583976823
2212	5	-0.2151431896451257
2212	6	-0.6137788823817658
2212	7	-0.45077013273350985
2212	8	-0.29340492727236267
2212	9	-0.21895195148302804
2212	10	-0.5380496012879021
2212	11	-0.4177838532285918
2212	12	-0.6828895665663879
2212	13	-0.6994078135138082
2212	14	-0.5204106270921471
2212	15	-0.6967342460041195
2212	16	-0.5255992591852703
2212	17	-0.4108332620325683
2212	18	-0.3272343999730083
2212	19	-0.5002983379768082
2212	20	-0.5243204993912443
2212	21	-0.3510047216609209
2212	22	-0.5250152692308916
2212	23	0.016610212506819266
2212	24	-0.25566433464614846
2212	25	-0.9809224706582285
2213	1	-0.10166423526909085
2213	2	-1.2690683300606551
2213	3	-0.24819906032557526
2213	4	0.2105544664875012
2213	5	0.21747444174642538
2213	6	0.056625948781750424
2213	7	0.16344205996713868
2213	8	0.2911902016676251
2213	9	0.20972830805884257
2213	10	-0.3169453294458009
2213	11	0.056479224018737284
2213	12	0.03037672826489768
2213	13	-0.32157038129658133
2213	14	0.06638654790545928
2213	15	0.05201362297247414
2213	16	0.028815474289601184
2213	17	0.16041263550661
2213	18	0.15265896768782028
2213	19	0.17742839520970932
2213	20	0.21285050581346354
2213	21	-0.4179846171265851
2213	22	-0.8210923564231791
2213	23	-0.014652616281615849
2213	24	0.08188162199488858
2213	25	-0.3393264895840318
2214	1	-0.1516458921892827
2214	2	-0.45672108535551104
2214	3	-0.35017239740218997
2214	4	-0.5346203950807364
2214	5	-0.18634970461591394
2214	6	-0.14314587027434555
2214	7	-0.4660945545125941
2214	8	-0.08755177416829696
2214	9	0.03533950346017889
2214	10	-1.9346552618875208
2214	11	-0.8303674313185212
2214	12	-0.41814262181762984
2214	13	-0.21412068912183457
2214	14	-0.6505078019281783
2214	15	-0.47771573073387685
2214	16	-0.6817377574773289
2214	17	-0.5559099553028304
2214	18	-0.4574912887668532
2214	19	-1.439226499032607
2214	20	-2.4200187252008263
2214	21	-1.8905909572117636
2214	22	-2.021191339365359
2214	23	-2.578380631963583
2214	24	-1.593419172158427
2214	25	-1.8123733622507032
2215	1	-0.2212178084133427
2215	2	-0.4026876410998277
2215	3	-0.884989864944199
2215	4	-0.24442684758773936
2215	5	-0.20479528435009797
2215	6	-0.15058415580178774
2215	7	-0.188173859768213
2215	8	-0.19405539542738381
2215	9	-0.18943413117176533
2215	10	-0.2560264461564117
2215	11	-0.5796879263252706
2215	12	-0.3220118299973578
2215	13	-0.3332746208182584
2215	14	-0.1785473554237953
2215	15	-0.6255181485356189
2215	16	-0.43529768320059503
2215	17	-0.2245715533262782
2215	18	-0.25170265533410685
2215	19	-2.165522418369463
2215	20	-2.458748949170918
2215	21	-3.379050234418216
2215	22	-2.4532651548948294
2215	23	-3.6500566726986747
2215	24	-2.460323745685862
2215	25	-1.838855454094805
2216	1	-1.5381496160681216
2216	2	-1.1836776410789298
2216	3	-0.8379047525942731
2216	4	-0.8864671692129562
2216	5	-1.9177421222717754
2216	6	-1.5098102763804064
2216	7	-1.161404030454392
2216	8	-5.284985730373339
2216	9	-4.247584284024352
2216	10	-2.259734401247215
2216	11	-1.1532176537614305
2216	12	-1.275716862845833
2216	13	-2.0908948188506713
2216	14	-3.550582712086481
2216	15	-2.058358529454182
2216	16	-3.021085948095944
2216	17	-2.8271583108384846
2216	18	-2.825098523175811
2216	19	-1.6759304134218524
2216	20	-0.9652688738929456
2216	21	-0.792943340630103
2216	22	-0.7348369122173892
2216	23	-1.0628494897700378
2216	24	-0.615361808604554
2216	25	-0.3426611760795737
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	10.0
7	162.0
8	860.0
9	1950.0
10	3409.0
11	5828.0
12	9281.0
13	13086.0
14	16915.0
15	21027.0
16	23628.0
17	25454.0
18	26455.0
19	27274.0
20	28385.0
21	30368.0
22	32857.0
23	35180.0
24	38381.0
25	41546.0
26	44993.0
27	51205.0
28	58205.0
29	69544.0
30	82832.0
31	101333.0
32	135771.0
33	190615.0
34	274879.0
35	429113.0
36	732143.0
37	2241791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.632238690877195	19.080219094010005	12.927176674537154	24.360365540575643
2	16.183026261936817	18.88279441993816	39.83854501518842	25.0956343029366
3	19.7708517654461	23.34494838938886	27.97762632703389	28.90657351813115
4	13.298229107230963	15.49602626141035	35.94116426003136	35.264580371327334
5	14.287052459760599	37.16004716571757	34.293699220914476	14.259201153607359
6	34.44985109179533	36.05543553016307	15.842635619943957	13.652077758097647
7	29.28268005688367	31.436300175248093	20.508219473521518	18.772800294346716
8	26.127408978390427	36.913466584458895	18.758202352933324	18.200922084217357
9	25.05749504727046	17.10728473616521	20.207929122884007	37.62729109368032
10	15.7264484134723	29.605241614124477	32.07637408719046	22.59193588521277
11	34.931374274885016	24.246226648857043	21.51392078945239	19.308478286805556
12	22.285869947693822	25.76979899550943	29.790639703095636	22.153691353701117
13	28.198556933304943	22.043800096488113	25.470170969175744	24.28747200103121
14	21.448664631115648	21.69524176218176	26.460675411095718	30.395418195606872
15	23.878953299706467	29.718580980526998	22.644070214695077	23.75839550507146
16	23.45705226263682	29.375003389397797	24.319148199489316	22.848796148476065
17	22.077770656011968	28.881800958998028	26.518429185510783	22.52199919947922
18	22.530738021203124	28.41161374507028	27.166866761722247	21.890781472004353
19	22.920325834060733	27.65021749037692	27.437221879813496	21.992234795748846
20	23.412688811670137	27.39445109563955	27.16476933794421	22.028090754746103
21	23.57842204053278	27.07220735975846	26.972637202249743	22.37673339745902
22	23.097605777861276	26.831089402383785	27.35316342079446	22.718141398960487
23	23.14383726920601	27.073023289600794	27.28575078549069	22.4973886557025
24	23.080245843665637	26.89156518951475	27.185468801394286	22.84272016542533
25	23.230878452281008	26.971295518933402	26.815321989905307	22.982504038880286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2761.0
1	2761.0
2	3079.5
3	3398.0
4	3398.0
5	3398.0
6	4783.0
7	6168.0
8	6168.0
9	6168.0
10	9243.0
11	12318.0
12	12318.0
13	12318.0
14	20328.0
15	28338.0
16	28338.0
17	28338.0
18	46109.0
19	63880.0
20	63880.0
21	63880.0
22	94306.0
23	124732.0
24	124732.0
25	124732.0
26	167124.0
27	209516.0
28	209516.0
29	209516.0
30	260432.5
31	311349.0
32	311349.0
33	311349.0
34	357317.0
35	403285.0
36	403285.0
37	403285.0
38	442125.0
39	480965.0
40	480965.0
41	480965.0
42	503187.5
43	525410.0
44	525410.0
45	525410.0
46	542975.5
47	560541.0
48	560541.0
49	560541.0
50	554110.5
51	547680.0
52	547680.0
53	547680.0
54	519940.5
55	492201.0
56	492201.0
57	492201.0
58	447299.5
59	402398.0
60	402398.0
61	402398.0
62	346776.0
63	291154.0
64	291154.0
65	291154.0
66	232730.0
67	174306.0
68	174306.0
69	174306.0
70	132072.5
71	89839.0
72	89839.0
73	89839.0
74	65389.5
75	40940.0
76	40940.0
77	40940.0
78	28134.5
79	15329.0
80	15329.0
81	15329.0
82	10428.0
83	5527.0
84	5527.0
85	5527.0
86	3634.0
87	1741.0
88	1741.0
89	1741.0
90	1137.5
91	534.0
92	534.0
93	534.0
94	334.5
95	135.0
96	135.0
97	135.0
98	85.0
99	35.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03063940197894245
2	0.014558408836829021
3	0.020627888738716193
4	0.02367305734928501
5	0.024361348884550566
6	0.012243246400026699
7	0.002794880779563164
8	0.002586307587058451
9	0.0032120271645725918
10	0.0016477282207872387
11	0.0014182977090320536
12	0.004359179723348518
13	0.0022317331598004373
14	7.71720812267441E-4
15	0.0020648746057966663
16	0.0024611636715556225
17	0.002106589244297609
18	0.0019188733710433666
19	3.1285978875707064E-4
20	4.171463850094275E-4
21	0.0810932572458327
22	0.07884066676678181
23	0.08017553519881197
24	0.0010637232817740402
25	0.22892993609317383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4794480.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.83624676877829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.961854106178	45.452722473112004
2	13.814651480761	16.53233790054967
3	4.800382926483629	8.617106921211134
4	2.002238906156853	4.792258451154006
5	1.066761964904526	3.1915516087787
6	0.6073517109603578	2.18049881114782
7	0.3973271079536651	1.664219401560836
8	0.25777666619379863	1.2339510567684095
9	0.1819436283434532	0.9798141459209174
>10	0.825287394108574	8.693238431268743
>50	0.05688227487130554	2.3653819113973618
>100	0.02574943462046822	2.6651364210736075
>500	8.434823340948884E-4	0.3446945408811653
>1k	8.434810046737889E-4	0.9090410715245186
>5k	1.0543512558401938E-4	0.37804685365112245
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6323	0.1318808296207305	No Hit
CCCATGTACTCTGCGTTGATACCAC	5885	0.12274532378902403	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5769	0.12032587475596937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.171463850094275E-5	0.0
4	0.0	0.0	0.0	4.171463850094275E-5	0.0
5	0.0	2.0857319250471376E-5	0.0	6.257195775141413E-5	0.0
6	0.0	2.0857319250471376E-5	0.0	6.257195775141413E-5	0.0
7	0.0	2.0857319250471376E-5	0.0	6.257195775141413E-5	0.0
8	0.0	2.0857319250471376E-5	0.0	6.257195775141413E-5	0.0
9	0.0	2.0857319250471376E-5	0.0	8.34292770018855E-5	0.0
10	0.0	2.0857319250471376E-5	0.0	1.4600123475329963E-4	0.0
11	0.0	2.0857319250471376E-5	0.0	1.4600123475329963E-4	0.0
12	0.0	2.0857319250471376E-5	0.0	1.4600123475329963E-4	0.0
13	0.0	2.0857319250471376E-5	0.0	1.4600123475329963E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	75	9.727592E-7	13.927888	7
ACCGTTC	135	5.730908E-8	10.55132	8
AGACCGT	165	9.367795E-10	10.35991	6
TCGGACG	85	6.623947E-4	10.054577	9
GAGTCGT	105	4.1253305E-5	9.948803	6
ACGGGTC	115	1.0292879E-5	9.911651	5
CGAATTA	135	6.427472E-7	9.85458	15
ACCGTCT	185	6.548362E-10	9.752841	8
TAGACTG	575	0.0	9.746456	5
CACACCG	275	0.0	9.671368	5
GTCGTAC	100	2.7542002E-4	9.5011425	1
GTCTTAG	480	0.0	9.5011425	1
CCGATCA	90	0.001118717	9.49599	9
CCGTGCG	130	4.2664597E-6	9.49599	9
TCGTCCG	90	0.001118717	9.49599	9
TCGTTTG	100	2.769536E-4	9.495891	13
CGGACGT	90	0.0011188237	9.495891	10
TCGTCTA	160	6.691698E-8	9.495791	14
CTACACT	795	0.0	9.439319	4
GTCTTAC	470	0.0	9.29899	1
>>END_MODULE