FastQCFastQC Report
Wed 25 May 2016
SRR1294738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794480
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167430.3492140962106422No Hit
GGTATCAACGCAGAGTACTTTTTTT128180.2673491181525421No Hit
TATCAACGCAGAGTACTTTTTTTTT112860.23539570506081994No Hit
GTACATGGGAAGCAGTGGTATCAAC66390.13847174250387947No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64010.1335077005222673No Hit
CCCATGTACTCTGCGTTGATACCAC63860.1331948407335102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG907.3492247E-611.628065
TCGCGCG1701.4006218E-1010.6117439
TACCGAT909.574462E-510.5500167
TATACCG1451.4137186E-810.4980615
TATCGCG1752.382876E-1010.3088737
GTTCTAG4550.010.2489161
CACGCGT750.002661775610.12759316
TATACTG12050.09.9481115
GTCGAAA1151.0345124E-59.90742915
TAGACAG7800.09.75781255
CTAGACT5700.09.5138674
ATACCGA1304.26977E-69.4954116
CGCGAGA1106.8852285E-59.49461714
TAGACTG5850.09.4325525
TCTATAC5900.09.3526153
GTCCTAA5500.09.3438171
CGTAGCA1856.542905E-99.2568322
GCGTCGC1252.775231E-59.11483414
TGGTCGG1151.1185814E-49.08171510
GTGTAGG7450.09.069751
Produced by FastQC (version 0.11.5)