FastQCFastQC Report
Wed 25 May 2016
SRR1294737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714475
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC98720.2657710712819443No Hit
CCCATGTACTCTGCGTTGATACCAC89870.2419453623998008No Hit
ATTCCATTCCATTCCATTCCATTCC58160.15657663599835778No Hit
GAATGGAATGGAATGGAATGGAATG50980.13724685184312724No Hit
GTATCAACGCAGAGTACTTTTTTTT49910.13436622941330875No Hit
GAGTACATGGGAAGCAGTGGTATCA46070.1240282947119041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1500.014.5688151
TCGTACC1550.013.4834992
CCCGATC751.4868852E-512.660868
CGTACCC1700.012.29361253
CGTAGAC1103.8218786E-812.0904113
GACGCTT1355.5193595E-810.578219
GTATAGG4200.010.4062971
GTATTAA5450.010.2859191
TGATCGA750.002659147110.12868810
CGACCAT1901.0004442E-109.9955516
GACTGCG1451.6300146E-79.823087
ATAGGAC3500.09.7710273
GTACGAC1951.6370905E-109.7431893
TACGACC2151.0913936E-119.7198754
TATTCCG2350.09.7001615
GTCCTAG4300.09.5014021
GTTAGCC2500.09.4996093
CGGACTG900.00111634819.4980755
ATCGTCC900.00111896299.49564558
GGTACGA2151.07320375E-109.2788142
Produced by FastQC (version 0.11.5)