FastQCFastQC Report
Wed 25 May 2016
SRR1294737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714475
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT150400.4049024424716817No Hit
GGTATCAACGCAGAGTACTTTTTTT115220.3101918844520424No Hit
GTACATGGGAAGCAGTGGTATCAAC100200.26975548361477736No Hit
TATCAACGCAGAGTACTTTTTTTTT100190.26972856190982575No Hit
CCCATGTACTCTGCGTTGATACCAC95960.2583406807153097No Hit
ATTCCATTCCATTCCATTCCATTCC65890.17738711392592493No Hit
GAATGGAATGGAATGGAATGGAATG58290.15692661816272824No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55670.1498731314654157No Hit
GAGTACATGGGAAGCAGTGGTATCA50700.13649304410448315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1950.014.6439961
GGGTACG1550.013.5102671
TCGTCCG500.001507242313.2917739
TCGTACC1900.012.5200772
TAGTACG550.003034068512.10983854
CGTACCC2200.011.244853
CGTATAC600.005810210511.1006853
ACGGCGT600.005904211711.07677658
GTCGTAG700.001468098510.8783971
TATGTCG1053.40541E-610.8741425
GTACGAC1855.456968E-1210.8006683
GGTACGA1605.311449E-1010.7046652
CGTAGAC803.7191794E-410.7042323
ATGTCGC1106.08154E-610.3593186
TACGACC1752.2919266E-1010.3304344
CGACCAT1752.382876E-1010.3099886
TAGGACG750.002614895310.1491994
CCGAGCG856.635171E-410.0526029
GTCCTAT3150.09.6696861
CTAGGAC3650.09.6452143
Produced by FastQC (version 0.11.5)