FastQCFastQC Report
Wed 25 May 2016
SRR1294736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662660
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1258013.4346895425728845No Hit
CCCATGTACTCTGCGTTGATACCAC1219533.3296292858195957No Hit
GAGTACATGGGAAGCAGTGGTATCA593621.6207346573255503No Hit
CATGTACTCTGCGTTGATACCACTG462451.2626069577847794No Hit
GCGTTGATACCACTGCTTCCCATGT411101.1224083043471138No Hit
GTATCAACGCAGAGTACATGGGAAG356530.9734182260979725No Hit
GCTTCCCATGTACTCTGCGTTGATA355530.9706879699453403No Hit
ACGCAGAGTACATGGGAAGCAGTGG310700.848290586622837No Hit
ACTCTGCGTTGATACCACTGCTTCC262240.7159823734662787No Hit
TATCAACGCAGAGTACATGGGAAGC256430.7001195852194853No Hit
GGTATCAACGCAGAGTACATGGGAA213970.5841929089787203No Hit
GTGGTATCAACGCAGAGTACATGGG172070.4697951761834295No Hit
CAGTGGTATCAACGCAGAGTACATG170950.4667372892924814No Hit
ATACCACTGCTTCCCATGTACTCTG140690.3841197381138298No Hit
GTACATGGTAAGCAGTGGTATCAAC140100.38250888698377683No Hit
GCAGTGGTATCAACGCAGAGTACAT138940.3793417898467234No Hit
GTACTCTGCGTTGATACCACTGCTT128440.3506741002440849No Hit
ACATGGGAAGCAGTGGTATCAACGC124050.3386882757340294No Hit
CATGGGAAGCAGTGGTATCAACGCA123090.3360672298275024No Hit
CTGCTTCCCATGTACTCTGCGTTGA122160.3335280916055544No Hit
GGGAAGCAGTGGTATCAACGCAGAG116490.3180475392201296No Hit
CCATGTACTCTGCGTTGATACCACT115790.3161363599132871No Hit
GATACCACTGCTTCCCATGTACTCT107630.293857469707808No Hit
CTTCCCATGTACTCTGCGTTGATAC106290.29019892646328077No Hit
TACCACTGCTTCCCATGTACTCTGC105050.2868134088340168No Hit
ACCATGTACTCTGCGTTGATACCAC103290.2820081580053841No Hit
GCAGAGTACATGGGAAGCAGTGGTA92310.2520299454494821No Hit
AAGCAGTGGTATCAACGCAGAGTAC92170.25164770958811356No Hit
CCACTGCTTCCCATGTACTCTGCGT91830.2507194224962186No Hit
GTTGATACCACTGCTTCCCATGTAC91160.248890150873955No Hit
ATGGGAAGCAGTGGTATCAACGCAG84890.23177144479695086No Hit
TTCCCATGTACTCTGCGTTGATACC81470.22243396875494859No Hit
GTACATGGGTAAGCAGTGGTATCAA79400.21678233851899986No Hit
TGATACCACTGCTTCCCATGTACTC70870.19349325353704686No Hit
CCCCATGTACTCTGCGTTGATACCA66280.18096137779646487No Hit
GGTAAGCAGTGGTATCAACGCAGAG63550.17350777849977886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA61420.16769233289467217No Hit
ATCAACGCAGAGTACATGGGAAGCA58020.15840946197572256No Hit
GCTTACCATGTACTCTGCGTTGATA54490.14877165775693074No Hit
GAGTACATGGTAAGCAGTGGTATCA48240.13170755680297924No Hit
AAAAAGTACTCTGCGTTGATACCAC40800.1113944510273954No Hit
GGAAGCAGTGGTATCAACGCAGAGT39000.10647998995265737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC307.7453145E-418.992758
CAAGACG350.002174313916.2819464
TATCGAC1207.185008E-1012.69232919
GTATCGA1207.348717E-1012.67429318
GTACAAA7650.011.0528991
CCGAATT803.7871138E-410.68327614
CTAGACA2600.010.2284014
GTACGAG750.00264782210.1338951
CGGTACT750.002654128210.1309894
CCTATAC1856.511982E-109.75476553
AAAAGTA9200.09.6016952
ATACGAC1002.7016472E-49.51924819
GGTATCG1606.582377E-89.50571917
GTCCTAG2800.09.5005251
AGACCGT1002.7665775E-49.4967646
CTATCGG900.00111816859.49637512
GTATTAG3450.09.3628371
GTCCTGG5600.09.33087351
GTGTAGA4100.09.2688061
ACGCAAA4050.09.1486551
Produced by FastQC (version 0.11.5)