##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294736_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3662660
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.057262481366003	33.0	33.0	33.0	27.0	33.0
2	31.01752578726936	33.0	33.0	33.0	27.0	33.0
3	31.08206549338459	33.0	33.0	33.0	27.0	33.0
4	34.4036025729934	37.0	37.0	37.0	27.0	37.0
5	34.81045851921827	37.0	37.0	37.0	27.0	37.0
6	34.97313755576548	37.0	37.0	37.0	33.0	37.0
7	35.02807795427367	37.0	37.0	37.0	33.0	37.0
8	34.90447762009032	37.0	37.0	37.0	33.0	37.0
9	34.6144389596632	37.0	37.0	37.0	27.0	37.0
10	34.73254083098076	37.0	37.0	37.0	27.0	37.0
11	34.98718445064516	37.0	37.0	37.0	33.0	37.0
12	34.92907558987184	37.0	37.0	37.0	33.0	37.0
13	34.79069310282691	37.0	37.0	37.0	33.0	37.0
14	36.043456668104604	40.0	37.0	40.0	27.0	40.0
15	36.26792931912872	40.0	37.0	40.0	27.0	40.0
16	36.24587157967161	40.0	37.0	40.0	27.0	40.0
17	36.25382836517722	40.0	37.0	40.0	27.0	40.0
18	36.229705459966254	40.0	37.0	40.0	27.0	40.0
19	36.21703133788012	40.0	37.0	40.0	27.0	40.0
20	36.15239361556901	40.0	37.0	40.0	27.0	40.0
21	36.08706568450252	40.0	37.0	40.0	27.0	40.0
22	36.009901546963135	40.0	37.0	40.0	27.0	40.0
23	35.65958674842874	37.0	37.0	40.0	27.0	40.0
24	36.005295058782416	37.0	37.0	40.0	27.0	40.0
25	34.92688292115566	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6320367328830372
1101	2	0.7110321636043366
1101	3	0.5661947967267196
1101	4	0.8092274316382557
1101	5	0.7720233260080107
1101	6	0.5857650953645788
1101	7	0.6181329387782242
1101	8	0.7338783421994108
1101	9	0.9119505336503835
1101	10	0.8659466797981565
1101	11	0.5554673095290568
1101	12	0.6699263724567359
1101	13	0.6667816104108724
1101	14	1.4127494901248525
1101	15	1.152500271519422
1101	16	1.0841658659146063
1101	17	1.1719778700773347
1101	18	1.1145745105589882
1101	19	1.1162711462137267
1101	20	1.1811234534808435
1101	21	1.2811495958293548
1101	22	1.3566674532124736
1101	23	1.592423163939273
1101	24	1.442199381483391
1101	25	1.274931329258223
1102	1	0.3319890846630358
1102	2	0.49688420777779996
1102	3	0.34866025176722104
1102	4	0.3039180585526893
1102	5	0.317815858527112
1102	6	0.3342155558019684
1102	7	0.36872627256534685
1102	8	0.4023828687803075
1102	9	0.6893691670216384
1102	10	0.6396873243780874
1102	11	0.43395462440599886
1102	12	0.43053814967051807
1102	13	0.40039564686338736
1102	14	1.131724178223493
1102	15	0.856882935346647
1102	16	0.5337741456842124
1102	17	0.8269246953120941
1102	18	0.8085880141293629
1102	19	0.7329564507895725
1102	20	0.8545425536101732
1102	21	0.8973801354211375
1102	22	0.9551005722908883
1102	23	1.3202756554225559
1102	24	1.0539694642357063
1102	25	0.945814377188448
1103	1	0.4392926170801488
1103	2	0.5982524279323584
1103	3	0.37123551546812905
1103	4	0.33668926554354783
1103	5	0.4212317192580457
1103	6	0.34951816504195676
1103	7	0.40176663416413305
1103	8	0.5276177467491507
1103	9	0.7171768215932133
1103	10	0.640206348044515
1103	11	0.4094661920471623
1103	12	0.5507137851715243
1103	13	0.46406641408047733
1103	14	1.1682832568168493
1103	15	0.8790712234327032
1103	16	0.7201339929595179
1103	17	0.6724357465642328
1103	18	0.8639600935015181
1103	19	0.7976901363213003
1103	20	0.8791043342881579
1103	21	0.9142204247254497
1103	22	0.878529142515049
1103	23	1.29597112078919
1103	24	0.9498711269029982
1103	25	0.9287349681482624
1104	1	0.41578940148133015
1104	2	0.5356335755673847
1104	3	0.3634646543866431
1104	4	0.46071702503214595
1104	5	0.40435581179049507
1104	6	0.3420560780721189
1104	7	0.3008356743603713
1104	8	0.4842293336544543
1104	9	0.6859502565732498
1104	10	0.6518358519104623
1104	11	0.4639807059119221
1104	12	0.45469557928210236
1104	13	0.5352792610129313
1104	14	0.9896027896494104
1104	15	0.8121077587625365
1104	16	0.7311412284444643
1104	17	0.7859873055358477
1104	18	0.6984247142657907
1104	19	0.8247767785024109
1104	20	0.8852518745657463
1104	21	0.9248079451659734
1104	22	1.03101145966734
1104	23	1.3220037552015356
1104	24	1.0779182557137545
1104	25	1.037437684829861
1105	1	0.2770476589402051
1105	2	0.33690927147559435
1105	3	0.27723355962143614
1105	4	0.3470958169441829
1105	5	0.2734517286943827
1105	6	0.28611091140183476
1105	7	0.24148369474325904
1105	8	0.38503837448541134
1105	9	-1.4310605161510637
1105	10	-0.10765363884335954
1105	11	0.17406519547058252
1105	12	0.28554465792353056
1105	13	0.34962815766431277
1105	14	-1.5962273837572312
1105	15	-0.14946191897927008
1105	16	-0.004152006415047538
1105	17	0.14782657301232405
1105	18	0.17494276930827368
1105	19	0.252376316193363
1105	20	0.47367404801584456
1105	21	0.673596624841764
1105	22	0.6276606697762048
1105	23	0.942278397197235
1105	24	0.7653800242224165
1105	25	0.6171950269059607
1106	1	-0.023088883960383555
1106	2	-0.0657367343881603
1106	3	-4.5292365701499193E-4
1106	4	-1.321155360456288
1106	5	-0.3580197347282521
1106	6	-0.09873415992593237
1106	7	0.03965462156665467
1106	8	0.07881421986169102
1106	9	0.31197798388722475
1106	10	0.29459521657569354
1106	11	0.30483558596823457
1106	12	0.04154481928620157
1106	13	0.20139887362226006
1106	14	0.5904446753397963
1106	15	0.24528285451130927
1106	16	0.10476837478373824
1106	17	-0.1570536220450549
1106	18	0.08083301944775201
1106	19	0.0703867487000096
1106	20	0.1894777434944075
1106	21	0.3860804202010826
1106	22	0.45674533321197686
1106	23	0.6837304834250659
1106	24	0.3370897642370778
1106	25	0.2569226863815004
1107	1	0.2467305990998483
1107	2	0.2619261126978252
1107	3	0.24412721206945065
1107	4	0.4236193120379852
1107	5	0.4140702146367303
1107	6	0.20639838788767406
1107	7	0.22959347409147313
1107	8	0.27494031408580355
1107	9	0.41285789607121615
1107	10	0.4962905436265146
1107	11	0.33152887562717126
1107	12	0.30512337316071125
1107	13	0.3311573088667146
1107	14	0.8322835344389148
1107	15	0.5847632576946893
1107	16	0.6090268854960783
1107	17	0.6064712041577849
1107	18	0.5899465506393042
1107	19	0.5300888651282349
1107	20	0.5784150109524475
1107	21	0.6460815630694299
1107	22	0.47555837682791235
1107	23	0.7859928081993175
1107	24	0.7056148162809848
1107	25	0.6771588341661143
1108	1	0.34376106313407817
1108	2	0.241332317653562
1108	3	0.39108791646149044
1108	4	0.5366843397546788
1108	5	0.4736165026523622
1108	6	0.3422802972255994
1108	7	0.29002619301558497
1108	8	0.33767314617420396
1108	9	0.050511038036106015
1108	10	0.46087024822330136
1108	11	0.2826822739432089
1108	12	0.2898409648208329
1108	13	0.3374195742046311
1108	14	-1.1268795302023378
1108	15	-0.04054323840866658
1108	16	0.286647695238365
1108	17	0.43095974079811583
1108	18	0.42067363464230567
1108	19	0.39576430987057876
1108	20	0.48843511288366415
1108	21	0.5431371014742865
1108	22	0.6975433940389806
1108	23	0.9439839623796473
1108	24	0.8330194438777028
1108	25	0.777691147368877
1109	1	0.4077098589586434
1109	2	0.4166069981197964
1109	3	0.5107612685458562
1109	4	0.5741950157130162
1109	5	0.4575651250541455
1109	6	0.30835336641099786
1109	7	0.32585744642049264
1109	8	0.43458353688260587
1109	9	0.6185558326520848
1109	10	0.5986546482273454
1109	11	0.36044205707191423
1109	12	0.32778822041905187
1109	13	0.4332157540732453
1109	14	0.7637197001607916
1109	15	0.4833265908178248
1109	16	0.445586774044024
1109	17	0.627677171182988
1109	18	0.5622452462367065
1109	19	0.42054251724630376
1109	20	0.6633996474668393
1109	21	0.7154897607309749
1109	22	0.8244649634943926
1109	23	1.147193908151749
1109	24	0.9334653083534832
1109	25	0.7453396159709058
1110	1	0.31138689804881636
1110	2	0.23745258607105768
1110	3	0.28027135323717545
1110	4	0.4343956814292298
1110	5	0.3408829674014697
1110	6	0.25978775842050794
1110	7	0.19755300813414323
1110	8	0.22880092216660586
1110	9	0.39627962639059433
1110	10	0.43200268016888543
1110	11	0.30113869978698204
1110	12	0.22165061054349167
1110	13	0.13043459713430394
1110	14	0.667952750753912
1110	15	0.36732687845096734
1110	16	0.3715989723040849
1110	17	0.3802962076238501
1110	18	0.49869490241407277
1110	19	0.3642169355469065
1110	20	0.5768862544001081
1110	21	0.5592332941852689
1110	22	0.5789254760400127
1110	23	0.6639645545953456
1110	24	0.6050463813796156
1110	25	0.6184855559162017
1111	1	0.21259895457742672
1111	2	0.07774058870677081
1111	3	0.27497394231428274
1111	4	0.48101519451846286
1111	5	0.3257360890734802
1111	6	0.3213571674501381
1111	7	0.23363164331765773
1111	8	0.33677963693192936
1111	9	0.29262060313553917
1111	10	0.3122661586133546
1111	11	0.1309656383465736
1111	12	0.2322208385929443
1111	13	0.17623033405492805
1111	14	-1.8458217473354281
1111	15	-0.5859427043593612
1111	16	-0.07968698162198962
1111	17	0.08377425766062174
1111	18	0.16405094401935116
1111	19	0.2050289484563308
1111	20	0.2318097700136832
1111	21	0.2793737572446915
1111	22	0.1390534797894034
1111	23	-1.7359094602026914
1111	24	-0.3289461894377581
1111	25	0.00392811664083581
1112	1	-0.23348407684880002
1112	2	-0.3288248339448643
1112	3	-0.019824414838556237
1112	4	0.09487944251912239
1112	5	-0.6473572646489103
1112	6	-0.8002898469384547
1112	7	-0.9217289170913148
1112	8	-0.704677817996199
1112	9	-0.595608350296267
1112	10	-0.8920034093520215
1112	11	-0.824632877869611
1112	12	-0.7100589819157719
1112	13	-0.8749586487672616
1112	14	-0.6024058193346917
1112	15	-0.8845682709888933
1112	16	-0.777911940527261
1112	17	-0.7654374681699707
1112	18	-0.8475405282832824
1112	19	-0.7491789970404739
1112	20	-0.7820908907541195
1112	21	-1.585026645482536
1112	22	-1.4943161579063684
1112	23	-1.218015113971667
1112	24	-1.486574109047524
1112	25	-1.9666850869178631
1113	1	0.0896907514469163
1113	2	-0.039622031917129874
1113	3	-0.04172757047375697
1113	4	0.23252860312919665
1113	5	0.0540192171005387
1113	6	-0.08583273638923572
1113	7	-8.335495818556637E-4
1113	8	0.0329310398503182
1113	9	0.24368350386357918
1113	10	-0.05532373281455705
1113	11	-0.23980333638199625
1113	12	-0.11796065083897389
1113	13	-0.16283477252851242
1113	14	0.03457716835407609
1113	15	-0.0413474449738942
1113	16	-0.008093152375508339
1113	17	0.016780990879055935
1113	18	0.011714034868440137
1113	19	-0.17832282342099148
1113	20	-0.735264199482323
1113	21	-0.5748360698128607
1113	22	-0.12639351621353967
1113	23	-0.3663928866690114
1113	24	-0.12812116567263132
1113	25	-1.3051052842973618
1114	1	-0.9519864391022281
1114	2	-0.6316862093205629
1114	3	-0.489924168270246
1114	4	-1.6490304892259573
1114	5	-0.7846622195609996
1114	6	-0.5916502441603768
1114	7	-0.4568535941207372
1114	8	-0.4146340568742559
1114	9	-0.42147579858319517
1114	10	-0.4088441912012257
1114	11	-0.48021839676290057
1114	12	-0.4638888722608243
1114	13	-0.622303398778655
1114	14	-0.6309674655558339
1114	15	-1.286977491617776
1114	16	-0.8610234331584579
1114	17	-0.8547146039411331
1114	18	-0.8166365074935698
1114	19	-0.7871741113902502
1114	20	-2.112227040378798
1114	21	-6.7805590363555694
1114	22	-7.840441737826136
1114	23	-7.7798484509973065
1114	24	-5.813483951798137
1114	25	-7.368099362356887
1115	1	-1.0529446830431581
1115	2	-1.0317510736954958
1115	3	-0.8784405326234221
1115	4	-0.7504976668930396
1115	5	-0.8357017802959987
1115	6	-1.0090920882993188
1115	7	-1.1697141311271366
1115	8	-0.9418764409750651
1115	9	-3.05736019986875
1115	10	-1.8331104737788593
1115	11	-1.3736596575951197
1115	12	-1.1863314316316789
1115	13	-1.5563153660195042
1115	14	-1.2959493915273157
1115	15	-2.1600349627607116
1115	16	-1.6972938789557688
1115	17	-3.348832228103376
1115	18	-3.1497105964834304
1115	19	-2.3689738294957365
1115	20	-2.109914137470035
1115	21	-1.5409022420208203
1115	22	-1.1391969284705397
1115	23	-0.5547670403681408
1115	24	-1.63378055131178
1115	25	-1.3419471195800696
1116	1	-1.724083572079003
1116	2	-0.9357461775833436
1116	3	-0.7211791867661681
1116	4	-0.4121004976388676
1116	5	-0.5419103812926522
1116	6	-0.5703605110211285
1116	7	-0.9490843024325883
1116	8	-2.0388400662364816
1116	9	-1.0883635269640664
1116	10	-2.794057770127573
1116	11	-1.3294941866018775
1116	12	-1.1091935522240561
1116	13	-1.0135107212841703
1116	14	-1.4890605027002977
1116	15	-1.4903438850507982
1116	16	-1.0611765315325599
1116	17	-0.9423855040907938
1116	18	-1.6104660189192828
1116	19	-1.2156292619833238
1116	20	-1.4064215087862877
1116	21	-0.8816438871802106
1116	22	-0.43598132105498166
1116	23	-0.2704226541210275
1116	24	-0.3974879250783161
1116	25	-0.4459698448467506
1201	1	0.48551850770187244
1201	2	0.45205985017673456
1201	3	0.43984040315040573
1201	4	0.6092189544577664
1201	5	0.5054515734871856
1201	6	0.4120994565560423
1201	7	0.47495260341490564
1201	8	0.5226812482576122
1201	9	0.8266340194468142
1201	10	0.7777547362481059
1201	11	0.5253130197981974
1201	12	0.5676413686394852
1201	13	0.5737533849320045
1201	14	1.2577820894251843
1201	15	1.0339713751827233
1201	16	0.9608773859487556
1201	17	0.9219885927014673
1201	18	0.7948765706652239
1201	19	0.763849523550185
1201	20	0.9309055949881966
1201	21	0.9633686088332496
1201	22	0.9767815318413469
1201	23	0.8717460087801285
1201	24	0.8510666636539099
1201	25	0.8239998233627972
1202	1	-0.10625125051121742
1202	2	0.21987771166065784
1202	3	0.1436950599519058
1202	4	0.19531334833876457
1202	5	0.20050063814580454
1202	6	0.09800023173671235
1202	7	0.1463897863933994
1202	8	0.24736900898640357
1202	9	0.3504263201564939
1202	10	0.5550632316126851
1202	11	0.26202441005528954
1202	12	0.2730432809682597
1202	13	0.3694459864812316
1202	14	0.672833808135259
1202	15	0.587831537729258
1202	16	0.6060450358850176
1202	17	0.3973239843364027
1202	18	0.36179904271651253
1202	19	0.36265872972057167
1202	20	0.5743239709063488
1202	21	0.6494379445156966
1202	22	0.611787129348798
1202	23	0.9143275820397108
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2211	5	0.25984302963328787
2211	6	0.22965172867842654
2211	7	0.16742909039568588
2211	8	0.3153362408828926
2211	9	0.4020275966348734
2211	10	0.42803722155611723
2211	11	0.15365759849310479
2211	12	0.3172001600110761
2211	13	0.1760278749154267
2211	14	0.45439214012696993
2211	15	0.3048655600963315
2211	16	0.21233226598435095
2211	17	0.28930992660034605
2211	18	0.3096301651763156
2211	19	0.23846704330393464
2211	20	0.3692813945865936
2211	21	0.3993249359929436
2211	22	0.36866660897294423
2211	23	0.5464347323404226
2211	24	0.5888230064636275
2211	25	0.5063288572958271
2212	1	-0.2712094586111
2212	2	-0.29717750892294603
2212	3	-0.22298979477729475
2212	4	-0.6396935915756004
2212	5	-0.29976090416125345
2212	6	-0.5425335421744961
2212	7	-0.518164829614939
2212	8	-0.18621367353944152
2212	9	-0.1374838003318004
2212	10	-0.48272475451154406
2212	11	-0.43305272155515695
2212	12	-0.6447855927334274
2212	13	-0.7041042013943368
2212	14	-0.5055093914599311
2212	15	-0.5523342073411683
2212	16	-0.46943369627493325
2212	17	-0.49562819223615406
2212	18	-0.4157794453779289
2212	19	-0.5000957761433042
2212	20	-0.4163890417887117
2212	21	-0.44868289331384403
2212	22	-0.46109487584953257
2212	23	0.023012534313828326
2212	24	-0.2928882444252494
2212	25	-0.9257619951595117
2213	1	-0.17612993410766364
2213	2	-1.350850875749341
2213	3	-0.277702118458091
2213	4	-0.0917513422599825
2213	5	-0.005237912403302403
2213	6	-0.04865131344130447
2213	7	-0.06357728115589367
2213	8	0.08997606966999427
2213	9	0.03157824881110827
2213	10	-0.49838370628585693
2213	11	-0.1607751625299727
2213	12	-0.15827926304391582
2213	13	-0.38053352517083283
2213	14	-0.11297997514395064
2213	15	-0.2591380905613434
2213	16	-0.18944217884193648
2213	17	-0.024407323370176925
2213	18	-0.07083593409659983
2213	19	-0.08469068847682593
2213	20	0.039563615073909375
2213	21	-0.43437728805727005
2213	22	-0.9246293377226777
2213	23	-0.22975675501113813
2213	24	-0.07515735036110982
2213	25	-0.6071670636217164
2214	1	-0.3329846637575109
2214	2	-0.5887532493167811
2214	3	-0.5687128716503018
2214	4	-0.7212614906318038
2214	5	-0.4521039948803889
2214	6	-0.5102697970512153
2214	7	-0.7196468435903256
2214	8	-0.32010773922999647
2214	9	-0.29541387317510726
2214	10	-2.174311642018921
2214	11	-1.0961445240213266
2214	12	-0.7307442805310274
2214	13	-0.4738109962748993
2214	14	-1.0402325629497255
2214	15	-0.7888823786798582
2214	16	-0.8527036282205813
2214	17	-0.8113249089172072
2214	18	-0.7875652641107536
2214	19	-1.6006570083189615
2214	20	-2.714007412743264
2214	21	-2.1393825449752626
2214	22	-2.208933916644476
2214	23	-2.7764848343164488
2214	24	-1.8581262323933387
2214	25	-2.2475462970014988
2215	1	-0.4396840203103132
2215	2	-0.4603941118103698
2215	3	-0.9609288270999663
2215	4	-0.5010894826759085
2215	5	-0.4105923769908344
2215	6	-0.405002968874534
2215	7	-0.4577783197257759
2215	8	-0.4324463024014449
2215	9	-0.30603330297198994
2215	10	-0.4064823656732841
2215	11	-0.7013463216252802
2215	12	-0.5991560928013513
2215	13	-0.5654358321824802
2215	14	-0.3966051983774648
2215	15	-0.7343146448426694
2215	16	-0.6233186072916155
2215	17	-0.4399683460778121
2215	18	-0.4713374283491234
2215	19	-2.5167549972641794
2215	20	-2.6945294754628506
2215	21	-3.4852805534087494
2215	22	-2.548754415535612
2215	23	-3.7755889388538293
2215	24	-2.6986297091243188
2215	25	-2.0874012192303653
2216	1	-1.7026180783880847
2216	2	-1.3609365979543568
2216	3	-1.12280323499877
2216	4	-1.215840782634622
2216	5	-2.134458815507024
2216	6	-1.9520904299565132
2216	7	-1.4361077832637292
2216	8	-5.4924980611628875
2216	9	-4.355232188481509
2216	10	-2.2485164577300694
2216	11	-1.2332253743374864
2216	12	-1.3407874269852869
2216	13	-2.230612981712511
2216	14	-3.80435540510409
2216	15	-2.346823475481443
2216	16	-3.4310825274029426
2216	17	-3.097792474036929
2216	18	-3.0007819052238673
2216	19	-1.977054444109129
2216	20	-1.2559626724168353
2216	21	-1.1311223599856959
2216	22	-0.9367101721325639
2216	23	-1.3088909537815638
2216	24	-0.8651563695458293
2216	25	-0.6889049527523028
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	50.0
8	292.0
9	719.0
10	1523.0
11	2632.0
12	4489.0
13	6919.0
14	10424.0
15	14959.0
16	18569.0
17	20476.0
18	20959.0
19	21199.0
20	21587.0
21	23091.0
22	23616.0
23	24897.0
24	26752.0
25	28640.0
26	30374.0
27	34170.0
28	39015.0
29	46280.0
30	55664.0
31	68916.0
32	94176.0
33	134859.0
34	199786.0
35	320630.0
36	566087.0
37	1800908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.062131308717504	19.025262180467557	11.78733340616124	24.1252731046537
2	12.860280330065429	21.966324457297738	37.6370869505975	27.536308262039334
3	21.520573935858213	27.914381493015316	26.91435746250968	23.650687108616793
4	13.132565423065653	19.316827295012825	33.616277345573	33.934329936348526
5	12.208892083219098	39.60678790314917	34.57167636434734	13.61264364928439
6	32.56653551154593	33.118124254231596	18.264083247550055	16.051256986672417
7	25.035739482380897	33.385536458094435	22.178683214401946	19.40004084512272
8	26.55566573800337	35.976401540758346	20.965720638713574	16.50221208252471
9	26.479971583191382	18.21042908889563	20.308380255560156	35.00121907235283
10	16.516501758294563	31.511368848699302	31.533620557848984	20.438508835157155
11	29.403986600844917	27.481427907188817	19.869170121161932	23.245415370804334
12	23.61949705969242	30.55050290208276	28.242297289696612	17.58770274852821
13	28.369657187962193	22.314440633207198	23.09222972668578	26.223672452144825
14	21.432575181529145	26.12616326770208	25.786107542793644	26.655154007975135
15	30.16406163184656	25.29515339840391	21.581849113754842	22.95893585599468
16	19.43839827879953	29.311246778209778	30.29654121707221	20.953813725918483
17	25.157218795212433	29.622360904402946	27.072867665910096	18.147552634474525
18	25.370988382236458	26.431797685839577	28.290541560719067	19.906672371204895
19	23.015406054248686	30.8054466559877	28.822019897609678	17.357127392153934
20	23.479227858766585	27.792503015579445	27.26943854251817	21.4588305831358
21	19.835656729578755	29.560311253213072	29.756340553226714	20.84769146398146
22	21.89045006604233	28.990505971133736	23.16760543137363	25.951438531450304
23	18.575848093960875	30.199773972767435	27.229450430304265	23.99492750296743
24	21.11655044749387	34.137639176327326	24.58122873789801	20.164581638280794
25	22.26673930716615	26.865692878996377	22.741492948335036	28.12607486550243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8149.0
1	8149.0
2	10092.5
3	12036.0
4	12036.0
5	12036.0
6	14567.0
7	17098.0
8	17098.0
9	17098.0
10	18313.0
11	19528.0
12	19528.0
13	19528.0
14	23722.0
15	27916.0
16	27916.0
17	27916.0
18	38587.5
19	49259.0
20	49259.0
21	49259.0
22	67788.0
23	86317.0
24	86317.0
25	86317.0
26	111798.0
27	137279.0
28	137279.0
29	137279.0
30	164996.0
31	192713.0
32	192713.0
33	192713.0
34	216615.0
35	240517.0
36	240517.0
37	240517.0
38	263309.5
39	286102.0
40	286102.0
41	286102.0
42	332758.5
43	379415.0
44	379415.0
45	379415.0
46	622386.5
47	865358.0
48	865358.0
49	865358.0
50	747316.0
51	629274.0
52	629274.0
53	629274.0
54	464859.5
55	300445.0
56	300445.0
57	300445.0
58	237337.0
59	174229.0
60	174229.0
61	174229.0
62	145012.5
63	115796.0
64	115796.0
65	115796.0
66	90964.0
67	66132.0
68	66132.0
69	66132.0
70	49393.0
71	32654.0
72	32654.0
73	32654.0
74	23498.5
75	14343.0
76	14343.0
77	14343.0
78	9823.5
79	5304.0
80	5304.0
81	5304.0
82	3609.5
83	1915.0
84	1915.0
85	1915.0
86	1274.5
87	634.0
88	634.0
89	634.0
90	410.5
91	187.0
92	187.0
93	187.0
94	113.0
95	39.0
96	39.0
97	39.0
98	30.0
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028940715217901744
2	0.013023321848055783
3	0.017692059869056916
4	0.01875685976858349
5	0.0168183779002146
6	0.009009845303686391
7	0.0012013127071581855
8	0.0013105229532634753
9	0.0010647998995265736
10	5.46051230526448E-4
11	0.0011194050225792184
12	0.0032217022601060434
13	0.002702953591105918
14	6.8256403815806E-4
15	0.0019930869914215354
16	0.00273025615263224
17	0.0024299279758426937
18	0.0021569023605794695
19	2.457230537369016E-4
20	3.822358613685136E-4
21	0.08198959226354617
22	0.07879519256496643
23	0.07983268990296669
24	0.001092102461052896
25	0.2220517328935801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3662660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.93591307527071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21331458060055	48.46078981881383
2	8.22674462510546	9.038874552344883
3	1.6911377738262858	2.7871259322368296
4	0.6226924280556103	1.368327084011691
5	0.33551812572164774	0.9215997294911096
6	0.19262898252143804	0.6349349423745336
7	0.1292151451263896	0.49689863864702793
8	0.08312142883677534	0.36530812714155125
9	0.0693002501397075	0.342636526599254
>10	0.37828975939812426	3.8827699056600538
>50	0.030344232698709155	1.136870981482633
>100	0.020116422131371218	2.2188515098406794
>500	0.002626431890541272	1.0337496404648845
>1k	0.0029799900295244654	3.440347438467551
>5k	6.566079726070855E-4	2.741034785799188
>10k+	0.001313215945214171	21.129880386624443
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	125801	3.4346895425728845	No Hit
CCCATGTACTCTGCGTTGATACCAC	121953	3.3296292858195957	No Hit
GAGTACATGGGAAGCAGTGGTATCA	59362	1.6207346573255503	No Hit
CATGTACTCTGCGTTGATACCACTG	46245	1.2626069577847794	No Hit
GCGTTGATACCACTGCTTCCCATGT	41110	1.1224083043471138	No Hit
GTATCAACGCAGAGTACATGGGAAG	35653	0.9734182260979725	No Hit
GCTTCCCATGTACTCTGCGTTGATA	35553	0.9706879699453403	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	31070	0.848290586622837	No Hit
ACTCTGCGTTGATACCACTGCTTCC	26224	0.7159823734662787	No Hit
TATCAACGCAGAGTACATGGGAAGC	25643	0.7001195852194853	No Hit
GGTATCAACGCAGAGTACATGGGAA	21397	0.5841929089787203	No Hit
GTGGTATCAACGCAGAGTACATGGG	17207	0.4697951761834295	No Hit
CAGTGGTATCAACGCAGAGTACATG	17095	0.4667372892924814	No Hit
ATACCACTGCTTCCCATGTACTCTG	14069	0.3841197381138298	No Hit
GTACATGGTAAGCAGTGGTATCAAC	14010	0.38250888698377683	No Hit
GCAGTGGTATCAACGCAGAGTACAT	13894	0.3793417898467234	No Hit
GTACTCTGCGTTGATACCACTGCTT	12844	0.3506741002440849	No Hit
ACATGGGAAGCAGTGGTATCAACGC	12405	0.3386882757340294	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12309	0.3360672298275024	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	12216	0.3335280916055544	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	11649	0.3180475392201296	No Hit
CCATGTACTCTGCGTTGATACCACT	11579	0.3161363599132871	No Hit
GATACCACTGCTTCCCATGTACTCT	10763	0.293857469707808	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10629	0.29019892646328077	No Hit
TACCACTGCTTCCCATGTACTCTGC	10505	0.2868134088340168	No Hit
ACCATGTACTCTGCGTTGATACCAC	10329	0.2820081580053841	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9231	0.2520299454494821	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9217	0.25164770958811356	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9183	0.2507194224962186	No Hit
GTTGATACCACTGCTTCCCATGTAC	9116	0.248890150873955	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8489	0.23177144479695086	No Hit
TTCCCATGTACTCTGCGTTGATACC	8147	0.22243396875494859	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7940	0.21678233851899986	No Hit
TGATACCACTGCTTCCCATGTACTC	7087	0.19349325353704686	No Hit
CCCCATGTACTCTGCGTTGATACCA	6628	0.18096137779646487	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6355	0.17350777849977886	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6142	0.16769233289467217	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5802	0.15840946197572256	No Hit
GCTTACCATGTACTCTGCGTTGATA	5449	0.14877165775693074	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4824	0.13170755680297924	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4080	0.1113944510273954	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	3900	0.10647998995265737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.73025615263224E-5	0.0
2	0.0	0.0	0.0	2.73025615263224E-5	0.0
3	0.0	0.0	0.0	2.73025615263224E-5	0.0
4	0.0	0.0	0.0	2.73025615263224E-5	0.0
5	0.0	0.0	0.0	2.73025615263224E-5	0.0
6	0.0	0.0	0.0	2.73025615263224E-5	0.0
7	0.0	0.0	0.0	2.73025615263224E-5	0.0
8	0.0	0.0	0.0	2.73025615263224E-5	0.0
9	0.0	0.0	0.0	2.73025615263224E-5	0.0
10	0.0	0.0	0.0	1.911179306842568E-4	0.0
11	0.0	0.0	0.0	2.457230537369016E-4	0.0
12	0.0	0.0	0.0	3.822358613685136E-4	0.0
13	0.0	0.0	0.0	3.822358613685136E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTC	30	7.7453145E-4	18.99275	8
CAAGACG	35	0.0021743139	16.281946	4
TATCGAC	120	7.185008E-10	12.692329	19
GTATCGA	120	7.348717E-10	12.674293	18
GTACAAA	765	0.0	11.052899	1
CCGAATT	80	3.7871138E-4	10.683276	14
CTAGACA	260	0.0	10.228401	4
GTACGAG	75	0.002647822	10.133895	1
CGGTACT	75	0.0026541282	10.130989	4
CCTATAC	185	6.511982E-10	9.7547655	3
AAAAGTA	920	0.0	9.601695	2
ATACGAC	100	2.7016472E-4	9.519248	19
GGTATCG	160	6.582377E-8	9.505719	17
GTCCTAG	280	0.0	9.500525	1
AGACCGT	100	2.7665775E-4	9.496764	6
CTATCGG	90	0.0011181685	9.496375	12
GTATTAG	345	0.0	9.362837	1
GTCCTGG	560	0.0	9.3308735	1
GTGTAGA	410	0.0	9.268806	1
ACGCAAA	405	0.0	9.148655	1
>>END_MODULE