FastQCFastQC Report
Wed 25 May 2016
SRR1294736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662660
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1319413.602327270344504No Hit
GTACATGGGAAGCAGTGGTATCAAC1239513.3841798037491877No Hit
GAGTACATGGGAAGCAGTGGTATCA639021.744688286655054No Hit
CATGTACTCTGCGTTGATACCACTG518111.4145730152402898No Hit
GCGTTGATACCACTGCTTCCCATGT439231.1992104099206586No Hit
ACGCAGAGTACATGGGAAGCAGTGG387391.0576739309682035No Hit
GCTTCCCATGTACTCTGCGTTGATA386611.0555443311691504No Hit
GTATCAACGCAGAGTACATGGGAAG379931.0373062200695669No Hit
ACTCTGCGTTGATACCACTGCTTCC293890.802394980697089No Hit
TATCAACGCAGAGTACATGGGAAGC290580.7933578328318763No Hit
GGTATCAACGCAGAGTACATGGGAA240230.655889435546843No Hit
CAGTGGTATCAACGCAGAGTACATG210160.5737906330371916No Hit
GTGGTATCAACGCAGAGTACATGGG187160.51099474152665No Hit
ACATGGGAAGCAGTGGTATCAACGC162570.4438577427334232No Hit
CATGGGAAGCAGTGGTATCAACGCA160930.43938012264310644No Hit
GCAGTGGTATCAACGCAGAGTACAT160580.43842453298968515No Hit
ATACCACTGCTTCCCATGTACTCTG154480.42176997045862846No Hit
GGGAAGCAGTGGTATCAACGCAGAG140130.38259079466835577No Hit
GTACATGGTAAGCAGTGGTATCAAC138360.37775824127819674No Hit
CCATGTACTCTGCGTTGATACCACT134890.36828425242856283No Hit
CTGCTTCCCATGTACTCTGCGTTGA128660.351274756597664No Hit
GTACTCTGCGTTGATACCACTGCTT125450.3425106343477145No Hit
CTTCCCATGTACTCTGCGTTGATAC120990.3303336919069747No Hit
GATACCACTGCTTCCCATGTACTCT117820.32167877990313054No Hit
TACCACTGCTTCCCATGTACTCTGC114600.3128873550916547No Hit
ACCATGTACTCTGCGTTGATACCAC111380.3040959302801789No Hit
AAGCAGTGGTATCAACGCAGAGTAC108910.2973521975831772No Hit
GCAGAGTACATGGGAAGCAGTGGTA105340.28760518311828015No Hit
ATGGGAAGCAGTGGTATCAACGCAG102580.28006967613701517No Hit
CCACTGCTTCCCATGTACTCTGCGT102400.27957823002954135No Hit
GTATCAACGCAGAGTACTTTTTTTT98880.2699677283722759No Hit
GTTGATACCACTGCTTCCCATGTAC96650.263879257151906No Hit
GTACATGGGTAAGCAGTGGTATCAA87660.23933425433974215No Hit
TTCCCATGTACTCTGCGTTGATACC84450.23057013208979266No Hit
GGTAAGCAGTGGTATCAACGCAGAG76270.20823663676126097No Hit
TATCAACGCAGAGTACTTTTTTTTT73550.20081034002610126No Hit
CCCCATGTACTCTGCGTTGATACCA72450.1978070582582058No Hit
TGATACCACTGCTTCCCATGTACTC72060.1967422583586792No Hit
GGTATCAACGCAGAGTACTTTTTTT71740.1958685763898369No Hit
ATCAACGCAGAGTACATGGGAAGCA68950.18825116172399295No Hit
GCTTACCATGTACTCTGCGTTGATA59390.16214991290482872No Hit
GAGTACATGGTAAGCAGTGGTATCA55260.15087395499445758No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46840.1278851981892941No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46410.12671118804366224No Hit
GGAAGCAGTGGTATCAACGCAGAGT46270.12632895218229373No Hit
AAAAAGTACTCTGCGTTGATACCAC45360.12384441908339841No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43070.11759213249387059No Hit
ATGGTAAGCAGTGGTATCAACGCAG37440.10222079035455106No Hit
ACTCTGCGTTGATACCACTGCTTAC37130.10137441094723507No Hit
CTGCGTTGATACCACTGCTTCCCAT36970.10093756996281392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG350.002149289516.3101865
TACGCAG550.003035483612.10907655
ACTGATC2550.011.1698868
GTCGTAG600.00579339511.1050011
TCGTAGA600.005812309711.100142
CTAGGAC2450.010.0967833
GAGCCGC951.6534232E-49.9950646
CTGATCG951.6547902E-49.9942459
TTAGACT1752.5120244E-99.7861124
ATGCGGG1106.696863E-59.5185731
TAGACAG4300.09.5142755
CTAGACT1951.6407284E-99.270324
AGGACTG3900.09.270325
CTACACT4050.09.1618964
TAGGCCC1356.7239816E-69.1618954
GCTAGAC1151.0933542E-49.1006113
CACACCG2002.588422E-99.0385625
TGCTCGG850.00747398478.9360310
TTTAGAC1502.6497746E-68.8799913
TAGGACA2800.08.8346844
Produced by FastQC (version 0.11.5)