FastQCFastQC Report
Wed 25 May 2016
SRR1294735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656297
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41470.15611959054277438No Hit
GTACATGGGAAGCAGTGGTATCAAC36600.1377857972960102No Hit
CCCATGTACTCTGCGTTGATACCAC33490.1260777691651197No Hit
GGTATCAACGCAGAGTACTTTTTTT30560.11504737610289813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG400.00528645114.2475433
GTACGAC500.00150198613.2977083
GTACCGT802.880424E-511.8700486
CTAGGAC2150.011.4863913
GCGCCTT600.00589744811.07829413
GTCCTAG3300.010.6526871
GTCCTAC1553.6598067E-910.4205021
GTCTAGG2251.8189894E-129.7121791
GTGTACG900.00111293999.5010451
TTACACC2700.09.4980044
GCACCGT800.00453279969.49603756
TGCGATT800.00453420789.4956812
CCAGTAC3150.09.3475943
AGTACTG4200.09.2715125
CTTAGGC2002.6666385E-99.0234443
TACATCG950.00182846218.997775
TAGACAG4450.08.964075
TGGACCG850.00745278748.9389615
TACACCG850.00745278748.9389615
CTAATAC3100.08.8855653
Produced by FastQC (version 0.11.5)