FastQCFastQC Report
Wed 25 May 2016
SRR1294735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656297
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115890.43628404504466184No Hit
GGTATCAACGCAGAGTACTTTTTTT95400.3591465863945184No Hit
TATCAACGCAGAGTACTTTTTTTTT78020.2937171558752655No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43650.16432650415220887No Hit
GTACATGGGAAGCAGTGGTATCAAC40290.1516773162037227No Hit
CCCATGTACTCTGCGTTGATACCAC36230.13639288076596856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTT250.00605400318.98866813
TCGACCG400.005300684414.2417699
TCGTTCT500.001506580213.2923179
CGCCCTA604.114376E-412.65911216
GACCGCC853.972693E-612.28678611
TGGCGTA550.003036109312.1085425
AAGACGG700.00147397510.8729775
AGTACCG750.002616798310.1481115
GTCCTAG2002.3646862E-119.9940721
CCGTGTG1651.0326403E-89.7822269
GGCGTAC800.00453317179.4959436
AACCGTG1002.7720045E-49.4946927
ACCGCCT900.00112015569.49433412
GGTATCA37650.09.379121
CTAGGAC2800.09.1740743
TATACTG7600.09.1383085
TAGACTG3550.09.1118615
CCTAGAC2850.09.0131253
AGGACCG950.00180031039.0131255
GTCTTAG2553.6379788E-128.9582711
Produced by FastQC (version 0.11.5)