FastQCFastQC Report
Wed 25 May 2016
SRR1294734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199637
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1196543.7396117122036032No Hit
CCCATGTACTCTGCGTTGATACCAC1102473.445609611340286No Hit
GAGTACATGGGAAGCAGTGGTATCA542811.696473693734633No Hit
CATGTACTCTGCGTTGATACCACTG400131.2505481090511206No Hit
GCTTCCCATGTACTCTGCGTTGATA377801.180758942342522No Hit
GCGTTGATACCACTGCTTCCCATGT365481.1422545745032953No Hit
GTATCAACGCAGAGTACATGGGAAG308080.9628592243432614No Hit
ACGCAGAGTACATGGGAAGCAGTGG283580.8862880382993445No Hit
TATCAACGCAGAGTACATGGGAAGC231740.7242696593394813No Hit
ACTCTGCGTTGATACCACTGCTTCC223960.6999544010773722No Hit
GGTATCAACGCAGAGTACATGGGAA196660.6146322223427221No Hit
CAGTGGTATCAACGCAGAGTACATG153960.48117958380903836No Hit
GTGGTATCAACGCAGAGTACATGGG153410.4794606388162157No Hit
GCAGTGGTATCAACGCAGAGTACAT137500.42973624820565587No Hit
GTACATGGTAAGCAGTGGTATCAAC122150.3817620561332426No Hit
ATACCACTGCTTCCCATGTACTCTG115090.35969705313446493No Hit
ACATGGGAAGCAGTGGTATCAACGC112300.35097731398905563No Hit
CATGGGAAGCAGTGGTATCAACGCA110890.3465705640983649No Hit
GGGAAGCAGTGGTATCAACGCAGAG108670.3396322770364263No Hit
CTGCTTCCCATGTACTCTGCGTTGA108550.33925723449253775No Hit
GTACTCTGCGTTGATACCACTGCTT108480.3390384596752694No Hit
GCAGAGTACATGGGAAGCAGTGGTA93780.2930957480489193No Hit
CTTCCCATGTACTCTGCGTTGATAC93170.2911892817841524No Hit
TACCACTGCTTCCCATGTACTCTGC88420.27634384775522974No Hit
GATACCACTGCTTCCCATGTACTCT86940.27171832304727067No Hit
AAGCAGTGGTATCAACGCAGAGTAC84050.26268604844862087No Hit
CCATGTACTCTGCGTTGATACCACT83770.26181094917954756No Hit
CCACTGCTTCCCATGTACTCTGCGT81500.2547163943909887No Hit
ACCATGTACTCTGCGTTGATACCAC80920.25290368876219393No Hit
GTTGATACCACTGCTTCCCATGTAC79780.2493407845952525No Hit
ATGGGAAGCAGTGGTATCAACGCAG76010.23755819800808653No Hit
GTACATGGGTAAGCAGTGGTATCAA73220.22883845886267723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA71210.22255649625254362No Hit
TTCCCATGTACTCTGCGTTGATACC66790.20874242921931457No Hit
CCCCATGTACTCTGCGTTGATACCA59150.18486472059174214No Hit
TGATACCACTGCTTCCCATGTACTC58660.18333329687086378No Hit
GGTAAGCAGTGGTATCAACGCAGAG53050.16580005794407304No Hit
GCTTACCATGTACTCTGCGTTGATA51550.16111202614546585No Hit
ATCAACGCAGAGTACATGGGAAGCA48100.1503295530086694No Hit
GAGTACATGGTAAGCAGTGGTATCA40430.12635808374512483No Hit
GGAAGCAGTGGTATCAACGCAGAGT37920.11851344386878887No Hit
AAAAAGTACTCTGCGTTGATACCAC36960.1155131035176803No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA36390.11373165143420956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA2200.013.3873432
GTACAAA7350.011.8944451
GTGTTAG2950.011.5964261
GCTAGAC1406.8030204E-1011.5360173
CTAGACA2500.011.3988744
TCTAGAC1451.2187229E-911.1382243
TACCGGG600.0058747611.0841452
GTACCGG700.001488711710.8601461
CTAGGAC2550.010.8042073
TAGACAG3050.010.5884565
TCAGGAC2250.010.5558343
ACGCAAA2950.010.3079351
GTCTAGG2151.8189894E-1210.1656011
TGGACCG951.647737E-49.9983875
GAACAAA13100.09.8653231
TAGGACA3100.09.8054834
TCTATAC3350.09.6420453
GTATCGA1606.598202E-89.50411318
CTTAGAC1701.6660124E-89.500253
AGGGCCG800.0045236679.4984685
Produced by FastQC (version 0.11.5)