##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294734_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3199637 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93748259568195 33.0 33.0 33.0 27.0 33.0 2 30.962071009930188 33.0 33.0 33.0 27.0 33.0 3 31.017604184474678 33.0 33.0 33.0 27.0 33.0 4 34.3837300918823 37.0 37.0 37.0 27.0 37.0 5 34.778773654636446 37.0 37.0 37.0 27.0 37.0 6 34.81921855510485 37.0 37.0 37.0 33.0 37.0 7 34.87755267238127 37.0 37.0 37.0 33.0 37.0 8 34.776575280258356 37.0 37.0 37.0 27.0 37.0 9 34.48776689355699 37.0 37.0 37.0 27.0 37.0 10 34.609902623328836 37.0 37.0 37.0 27.0 37.0 11 34.837271853025825 37.0 37.0 37.0 33.0 37.0 12 34.78011786962084 37.0 37.0 37.0 27.0 37.0 13 34.62393609025024 37.0 37.0 37.0 27.0 37.0 14 35.862915074428756 40.0 37.0 40.0 27.0 40.0 15 36.076232710148055 40.0 37.0 40.0 27.0 40.0 16 36.05041478142677 40.0 37.0 40.0 27.0 40.0 17 36.0596911462144 40.0 37.0 40.0 27.0 40.0 18 36.03870064010386 40.0 37.0 40.0 27.0 40.0 19 36.04136719259091 40.0 37.0 40.0 27.0 40.0 20 35.97163865776024 40.0 37.0 40.0 27.0 40.0 21 35.89350979501737 40.0 37.0 40.0 27.0 40.0 22 35.819878629982085 37.0 37.0 40.0 27.0 40.0 23 35.48609107845671 37.0 37.0 40.0 27.0 40.0 24 35.82230421763469 37.0 37.0 40.0 27.0 40.0 25 34.676804587520394 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43656031663059025 1101 2 0.532381638067779 1101 3 0.4258579858290119 1101 4 0.494113964953506 1101 5 0.5761179301493868 1101 6 0.23182408302538704 1101 7 0.2915642450502247 1101 8 0.4751217542193942 1101 9 0.6322501282454382 1101 10 0.7368683789141812 1101 11 0.4679154864027595 1101 12 0.47857429649636174 1101 13 0.4474070613417922 1101 14 1.200322670189415 1101 15 0.8744446141237461 1101 16 0.8033380322223564 1101 17 0.8355014934078042 1101 18 0.90983701514741 1101 19 0.887259912317198 1101 20 0.9290750484208488 1101 21 0.828510604547219 1101 22 0.9709190329903521 1101 23 1.3171902765870058 1101 24 1.0917216044988933 1101 25 1.0014928058686223 1102 1 0.35061924402991806 1102 2 0.5696814660831144 1102 3 0.43614737352872623 1102 4 0.4192405712745213 1102 5 0.34122001706396077 1102 6 0.40585963106007483 1102 7 0.3690707949732044 1102 8 0.47478259178427606 1102 9 0.6253767396711254 1102 10 0.6973918367630461 1102 11 0.5102877347225814 1102 12 0.6098642325221562 1102 13 0.5372910620887765 1102 14 1.2263845361740948 1102 15 0.9854628203360392 1102 16 0.5728928756771765 1102 17 0.8119439334205438 1102 18 0.8138949258676789 1102 19 0.7902376334331933 1102 20 0.9594130920836008 1102 21 0.8234673659096714 1102 22 0.9701998708019701 1102 23 1.3220843739355175 1102 24 1.0797814958290886 1102 25 0.9610373591700423 1103 1 0.2893115691338224 1103 2 0.5323461412647852 1103 3 0.30006490758045956 1103 4 0.25551796987717523 1103 5 0.3579072359762421 1103 6 0.2750921414503722 1103 7 0.3455132539552892 1103 8 0.4877912347689346 1103 9 0.6395454929302531 1103 10 0.634196316399759 1103 11 0.4345819445100645 1103 12 0.5252205994271648 1103 13 0.5493150040747352 1103 14 1.1378261896286048 1103 15 0.8282461715616236 1103 16 0.6064143661993029 1103 17 0.6750730040324413 1103 18 0.7143169119021877 1103 19 0.7746620927635846 1103 20 0.8452442384472079 1103 21 0.910695964597835 1103 22 0.8536342589498247 1103 23 1.3564171637259435 1103 24 1.0391086268510321 1103 25 0.8218261337794104 1104 1 0.40936512052648055 1104 2 0.5576331526105882 1104 3 0.4262150841190433 1104 4 0.4645853392948922 1104 5 0.34299198444195866 1104 6 0.4232657639040127 1104 7 0.308072286137012 1104 8 0.5500970108864038 1104 9 0.725710467948673 1104 10 0.6224200491597003 1104 11 0.4683060704959132 1104 12 0.3731298435283463 1104 13 0.4647101498741222 1104 14 0.9834362520687847 1104 15 0.8572924834379805 1104 16 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7 -0.44411835449718495 2212 8 -0.3484134673533035 2212 9 -0.22681804958402552 2212 10 -0.5930645423717209 2212 11 -0.44955300356767935 2212 12 -0.6096025418384627 2212 13 -0.5998724279623318 2212 14 -0.45069662459791715 2212 15 -0.6664929643902866 2212 16 -0.43545297117540116 2212 17 -0.5742211006083267 2212 18 -0.4152466844675402 2212 19 -0.6344408318308652 2212 20 -0.4674676397352968 2212 21 -0.5604893259871346 2212 22 -0.5223211370251946 2212 23 0.020488206965112 2212 24 -0.20372095119643774 2212 25 -0.8618590004221929 2213 1 -0.2453334751851841 2213 2 -1.272555364263507 2213 3 -0.4514186216286369 2213 4 -0.1054313968184033 2213 5 -0.14113483229151313 2213 6 -0.04839392967024736 2213 7 -0.1309770543745259 2213 8 -0.09719478954757932 2213 9 -0.02165797291965532 2213 10 -0.45749615002490174 2213 11 -0.25897672932602234 2213 12 -0.15440103018836027 2213 13 -0.494881643095205 2213 14 -0.12453624711972822 2213 15 -0.18237721085047554 2213 16 -0.09770869617389621 2213 17 0.009850594823781478 2213 18 -0.1312077989415812 2213 19 -0.05467805671976578 2213 20 -0.023353402395777323 2213 21 -0.7085751609018018 2213 22 -1.121505515134686 2213 23 -0.29499699880597774 2213 24 -0.1362171446865048 2213 25 -0.7492330811213606 2214 1 -0.17720834673567154 2214 2 -0.3511371526517557 2214 3 -0.3136582767505729 2214 4 -0.283331217938418 2214 5 -0.182354335224872 2214 6 -0.05594487684137306 2214 7 -0.3439463824426241 2214 8 -0.04331415041468745 2214 9 0.14702910131278202 2214 10 -1.7475871089474353 2214 11 -0.7358100897623885 2214 12 -0.3794252634842721 2214 13 -0.17023877984433256 2214 14 -0.5938622136470499 2214 15 -0.4508293728460018 2214 16 -0.5037961957644512 2214 17 -0.3591291268933361 2214 18 -0.3903818116884068 2214 19 -1.2639282188306566 2214 20 -2.353024167272224 2214 21 -1.9027188631870615 2214 22 -1.9317502206957897 2214 23 -2.32305406821785 2214 24 -1.5845599321938337 2214 25 -1.754387366921776 2215 1 -0.21678333019112017 2215 2 -0.20557926447795083 2215 3 -0.5159037056330256 2215 4 -0.27740507469341935 2215 5 -0.13672444648850046 2215 6 -0.16807211096495678 2215 7 -0.1077967650473326 2215 8 -0.1632550512946409 2215 9 -0.33312857756940417 2215 10 -0.2771612116514106 2215 11 -0.36996916531450097 2215 12 -0.25932283348051044 2215 13 -0.2121211667991858 2215 14 -0.1268434842755184 2215 15 -0.5469646144973979 2215 16 -0.37913078559013513 2215 17 -0.22027974514257664 2215 18 -0.1593487772419735 2215 19 -2.0700998846032306 2215 20 -2.2706741221728777 2215 21 -3.264906235578181 2215 22 -2.576493413757561 2215 23 -3.3506375159386366 2215 24 -2.0134279367904497 2215 25 -1.3161437977129893 2216 1 -1.4779539555011674 2216 2 -1.2227447567296252 2216 3 -0.9106783209060723 2216 4 -0.9183755450756124 2216 5 -1.884828212854515 2216 6 -1.6158438836922784 2216 7 -1.1610077055692116 2216 8 -5.184787597041904 2216 9 -4.025929056488771 2216 10 -1.9910650188018195 2216 11 -1.1423256322310635 2216 12 -1.273698954473332 2216 13 -1.9512090533910182 2216 14 -3.5795878011421323 2216 15 -2.1372366497943034 2216 16 -3.014670901834151 2216 17 -2.714318589964755 2216 18 -2.6867943522061353 2216 19 -1.7707362136077691 2216 20 -0.988554643529902 2216 21 -0.7600597625835945 2216 22 -0.7582351130437175 2216 23 -1.251210318674751 2216 24 -0.6769203188754815 2216 25 -0.5244636251087584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 34.0 8 236.0 9 661.0 10 1361.0 11 2578.0 12 4339.0 13 6754.0 14 9961.0 15 14498.0 16 18237.0 17 19789.0 18 20307.0 19 20489.0 20 21166.0 21 22148.0 22 23176.0 23 24167.0 24 25309.0 25 27019.0 26 28700.0 27 31527.0 28 35402.0 29 41714.0 30 49915.0 31 61053.0 32 82880.0 33 118083.0 34 172800.0 35 274416.0 36 482389.0 37 1558527.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.58907986235404 19.071630733177 11.498477009454954 23.84081239501401 2 12.556932255187785 22.190849766363247 37.57511650579817 27.677101472650794 3 21.160730361804923 28.743116753858615 26.94672079903172 23.14943208530474 4 12.922260810308126 19.455960087901644 33.94403928690885 33.677739814881384 5 11.698022144825988 40.41815223051825 34.47545920411913 13.408366420536636 6 32.124909906615436 33.33936560504935 18.36289146474184 16.172833023593373 7 24.73368072690746 33.67503876220959 22.334096024017725 19.257184486865228 8 26.303908169410782 36.65872720948135 21.097151515284107 15.940213105823764 9 26.478849293028293 18.42051885325598 20.37058625161073 34.730045602104994 10 16.394708648108722 32.229509856746205 31.544244407801386 19.831537087343683 11 28.96252571018878 28.109170907552084 19.843499529786 23.084803852473133 12 23.363924580761143 31.37956274643644 28.19639155796585 17.06012111483657 13 28.192000570079635 22.825219647869563 22.82625104194242 26.15652874010838 14 21.112500511779363 26.734385103735725 25.953792652317258 26.19932173216765 15 30.33792321662922 25.58869574158802 21.358850071556425 22.714530970226342 16 18.96115350572327 29.594432748421035 30.911798092742522 20.53261565311317 17 25.379465883309688 29.835574420631815 27.02659706966233 17.758362626396167 18 25.30440818747775 26.58889369161909 28.222926275382715 19.883771845520442 19 22.935916300269906 31.218629165640504 28.916205258861343 16.929249275228244 20 23.21851668149654 28.186758252456617 27.52705553122977 21.06766953481707 21 19.430886349611075 29.925680614622973 30.329728348430645 20.313704687335303 22 21.712002697298605 29.363617057411112 23.001351464200898 25.923028781089386 23 18.176759844891315 30.985486866493055 27.152996238633172 23.684757049982455 24 20.696899612451556 34.949806225778225 24.68702337792224 19.66627078384798 25 21.80239395863238 27.445457526606067 22.608492204459615 28.14365631030194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9434.0 1 9434.0 2 11389.5 3 13345.0 4 13345.0 5 13345.0 6 15648.5 7 17952.0 8 17952.0 9 17952.0 10 19021.5 11 20091.0 12 20091.0 13 20091.0 14 24282.5 15 28474.0 16 28474.0 17 28474.0 18 38525.5 19 48577.0 20 48577.0 21 48577.0 22 65058.5 23 81540.0 24 81540.0 25 81540.0 26 103268.5 27 124997.0 28 124997.0 29 124997.0 30 148151.5 31 171306.0 32 171306.0 33 171306.0 34 191740.0 35 212174.0 36 212174.0 37 212174.0 38 230190.5 39 248207.0 40 248207.0 41 248207.0 42 287537.5 43 326868.0 44 326868.0 45 326868.0 46 546028.0 47 765188.0 48 765188.0 49 765188.0 50 655191.5 51 545195.0 52 545195.0 53 545195.0 54 399821.5 55 254448.0 56 254448.0 57 254448.0 58 198460.0 59 142472.0 60 142472.0 61 142472.0 62 118319.5 63 94167.0 64 94167.0 65 94167.0 66 73416.5 67 52666.0 68 52666.0 69 52666.0 70 39018.5 71 25371.0 72 25371.0 73 25371.0 74 18254.5 75 11138.0 76 11138.0 77 11138.0 78 7607.0 79 4076.0 80 4076.0 81 4076.0 82 2737.0 83 1398.0 84 1398.0 85 1398.0 86 905.0 87 412.0 88 412.0 89 412.0 90 262.5 91 113.0 92 113.0 93 113.0 94 66.5 95 20.0 96 20.0 97 20.0 98 14.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03112853114275151 2 0.013751559942580987 3 0.017658253108086948 4 0.01897090201169695 5 0.016095575841884563 6 0.005594384613004538 7 0.0 8 1.875212719442862E-4 9 1.250141812961908E-4 10 3.12535453240477E-5 11 9.376063597214308E-4 12 9.063528143973833E-4 13 0.0026252978072200065 14 5.000567251847632E-4 15 0.0020002269007390527 16 0.002156494627359291 17 0.0023127623539795296 18 0.002125241082035243 19 2.812819079164293E-4 20 4.3754963453666775E-4 21 0.07782132785687876 22 0.07388338114604875 23 0.07485224105109424 24 0.0011563811769897648 25 0.21408678546972673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3199637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.58779655196333 #Duplication Level Percentage of deduplicated Percentage of total 1 90.49863446541568 51.2111831535944 2 6.77892470393542 7.672088239747516 3 1.2702061742878867 2.156345057089519 4 0.4735840741008618 1.0719631694187797 5 0.2314930487176137 0.654984077201303 6 0.14903363012508788 0.5060090844551416 7 0.09037328384721688 0.35798175000554 8 0.07416843569258987 0.3357622679639721 9 0.05879695005772416 0.2994470862968199 >10 0.3221987221719252 3.3410474256805283 >50 0.02493714181577129 0.9695794803811083 >100 0.019429473517629265 2.2304246728717914 >500 0.003075471410230027 1.2076387913519275 >1k 0.003019553748220318 3.4871697366895145 >5k 9.506002540693594E-4 4.056482906713722 >10k+ 0.0011742709020856794 20.441893100538483 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 119654 3.7396117122036032 No Hit CCCATGTACTCTGCGTTGATACCAC 110247 3.445609611340286 No Hit GAGTACATGGGAAGCAGTGGTATCA 54281 1.696473693734633 No Hit CATGTACTCTGCGTTGATACCACTG 40013 1.2505481090511206 No Hit GCTTCCCATGTACTCTGCGTTGATA 37780 1.180758942342522 No Hit GCGTTGATACCACTGCTTCCCATGT 36548 1.1422545745032953 No Hit GTATCAACGCAGAGTACATGGGAAG 30808 0.9628592243432614 No Hit ACGCAGAGTACATGGGAAGCAGTGG 28358 0.8862880382993445 No Hit TATCAACGCAGAGTACATGGGAAGC 23174 0.7242696593394813 No Hit ACTCTGCGTTGATACCACTGCTTCC 22396 0.6999544010773722 No Hit GGTATCAACGCAGAGTACATGGGAA 19666 0.6146322223427221 No Hit CAGTGGTATCAACGCAGAGTACATG 15396 0.48117958380903836 No Hit GTGGTATCAACGCAGAGTACATGGG 15341 0.4794606388162157 No Hit GCAGTGGTATCAACGCAGAGTACAT 13750 0.42973624820565587 No Hit GTACATGGTAAGCAGTGGTATCAAC 12215 0.3817620561332426 No Hit ATACCACTGCTTCCCATGTACTCTG 11509 0.35969705313446493 No Hit ACATGGGAAGCAGTGGTATCAACGC 11230 0.35097731398905563 No Hit CATGGGAAGCAGTGGTATCAACGCA 11089 0.3465705640983649 No Hit GGGAAGCAGTGGTATCAACGCAGAG 10867 0.3396322770364263 No Hit CTGCTTCCCATGTACTCTGCGTTGA 10855 0.33925723449253775 No Hit GTACTCTGCGTTGATACCACTGCTT 10848 0.3390384596752694 No Hit GCAGAGTACATGGGAAGCAGTGGTA 9378 0.2930957480489193 No Hit CTTCCCATGTACTCTGCGTTGATAC 9317 0.2911892817841524 No Hit TACCACTGCTTCCCATGTACTCTGC 8842 0.27634384775522974 No Hit GATACCACTGCTTCCCATGTACTCT 8694 0.27171832304727067 No Hit AAGCAGTGGTATCAACGCAGAGTAC 8405 0.26268604844862087 No Hit CCATGTACTCTGCGTTGATACCACT 8377 0.26181094917954756 No Hit CCACTGCTTCCCATGTACTCTGCGT 8150 0.2547163943909887 No Hit ACCATGTACTCTGCGTTGATACCAC 8092 0.25290368876219393 No Hit GTTGATACCACTGCTTCCCATGTAC 7978 0.2493407845952525 No Hit ATGGGAAGCAGTGGTATCAACGCAG 7601 0.23755819800808653 No Hit GTACATGGGTAAGCAGTGGTATCAA 7322 0.22883845886267723 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7121 0.22255649625254362 No Hit TTCCCATGTACTCTGCGTTGATACC 6679 0.20874242921931457 No Hit CCCCATGTACTCTGCGTTGATACCA 5915 0.18486472059174214 No Hit TGATACCACTGCTTCCCATGTACTC 5866 0.18333329687086378 No Hit GGTAAGCAGTGGTATCAACGCAGAG 5305 0.16580005794407304 No Hit GCTTACCATGTACTCTGCGTTGATA 5155 0.16111202614546585 No Hit ATCAACGCAGAGTACATGGGAAGCA 4810 0.1503295530086694 No Hit GAGTACATGGTAAGCAGTGGTATCA 4043 0.12635808374512483 No Hit GGAAGCAGTGGTATCAACGCAGAGT 3792 0.11851344386878887 No Hit AAAAAGTACTCTGCGTTGATACCAC 3696 0.1155131035176803 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 3639 0.11373165143420956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 9.37606359721431E-5 0.0 11 0.0 0.0 0.0 9.37606359721431E-5 0.0 12 0.0 0.0 0.0 1.250141812961908E-4 0.0 13 0.0 0.0 0.0 1.5626772662023848E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAAAA 220 0.0 13.387343 2 GTACAAA 735 0.0 11.894445 1 GTGTTAG 295 0.0 11.596426 1 GCTAGAC 140 6.8030204E-10 11.536017 3 CTAGACA 250 0.0 11.398874 4 TCTAGAC 145 1.2187229E-9 11.138224 3 TACCGGG 60 0.00587476 11.084145 2 GTACCGG 70 0.0014887117 10.860146 1 CTAGGAC 255 0.0 10.804207 3 TAGACAG 305 0.0 10.588456 5 TCAGGAC 225 0.0 10.555834 3 ACGCAAA 295 0.0 10.307935 1 GTCTAGG 215 1.8189894E-12 10.165601 1 TGGACCG 95 1.647737E-4 9.998387 5 GAACAAA 1310 0.0 9.865323 1 TAGGACA 310 0.0 9.805483 4 TCTATAC 335 0.0 9.642045 3 GTATCGA 160 6.598202E-8 9.504113 18 CTTAGAC 170 1.6660124E-8 9.50025 3 AGGGCCG 80 0.004523667 9.498468 5 >>END_MODULE