FastQCFastQC Report
Wed 25 May 2016
SRR1294734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199637
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1234253.857468831620587No Hit
GTACATGGGAAGCAGTGGTATCAAC1166633.6461323581393765No Hit
GAGTACATGGGAAGCAGTGGTATCA580301.8136432351544878No Hit
CATGTACTCTGCGTTGATACCACTG470681.471041871312277No Hit
GCTTCCCATGTACTCTGCGTTGATA405831.2683626298858277No Hit
GCGTTGATACCACTGCTTCCCATGT381701.1929478250189005No Hit
ACGCAGAGTACATGGGAAGCAGTGG348351.0887172513632015No Hit
GTATCAACGCAGAGTACATGGGAAG323781.0119272905020162No Hit
TATCAACGCAGAGTACATGGGAAGC259950.8124359106986199No Hit
ACTCTGCGTTGATACCACTGCTTCC258550.8080604143532532No Hit
GGTATCAACGCAGAGTACATGGGAA212670.6646691484065224No Hit
CAGTGGTATCAACGCAGAGTACATG184050.5752215016890979No Hit
GTGGTATCAACGCAGAGTACATGGG158900.4966188351991179No Hit
CATGGGAAGCAGTGGTATCAACGCA145330.4542077741943852No Hit
ACATGGGAAGCAGTGGTATCAACGC143370.44808207931087185No Hit
GCAGTGGTATCAACGCAGAGTACAT142940.4467381768619378No Hit
ATACCACTGCTTCCCATGTACTCTG132600.4144220109968725No Hit
GGGAAGCAGTGGTATCAACGCAGAG127100.3972325610686462No Hit
CTGCTTCCCATGTACTCTGCGTTGA119550.3736361343489902No Hit
GTACATGGTAAGCAGTGGTATCAAC119400.37316733116912953No Hit
GTACTCTGCGTTGATACCACTGCTT110870.3465080570077168No Hit
CTTCCCATGTACTCTGCGTTGATAC110800.3462892821904485No Hit
CCATGTACTCTGCGTTGATACCACT104430.3263807738190301No Hit
GATACCACTGCTTCCCATGTACTCT103970.3249431107341239No Hit
TACCACTGCTTCCCATGTACTCTGC100060.31272297451242126No Hit
AAGCAGTGGTATCAACGCAGAGTAC99440.3107852547023303No Hit
GCAGAGTACATGGGAAGCAGTGGTA95430.29825258302738716No Hit
CCACTGCTTCCCATGTACTCTGCGT95400.298158822391415No Hit
ACCATGTACTCTGCGTTGATACCAC94750.2961273419453519No Hit
ATGGGAAGCAGTGGTATCAACGCAG93450.29206438105322574No Hit
GTATCAACGCAGAGTACTTTTTTTT91130.2848135585380467No Hit
GTTGATACCACTGCTTCCCATGTAC87280.2727809435882883No Hit
GTACATGGGTAAGCAGTGGTATCAA78900.24659047260673633No Hit
CCCCATGTACTCTGCGTTGATACCA71220.22258774979786766No Hit
TTCCCATGTACTCTGCGTTGATACC70520.22040000162518433No Hit
TATCAACGCAGAGTACTTTTTTTTT68540.2142117996510229No Hit
GGTATCAACGCAGAGTACTTTTTTT65640.2051482715070491No Hit
GGTAAGCAGTGGTATCAACGCAGAG65390.2043669328739479No Hit
TGATACCACTGCTTCCCATGTACTC63120.19727237808538906No Hit
ATCAACGCAGAGTACATGGGAAGCA57060.17833272961901614No Hit
GCTTACCATGTACTCTGCGTTGATA56620.17695757362475806No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48700.15220476572811228No Hit
GGAAGCAGTGGTATCAACGCAGAGT45610.14254742022298156No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45420.14195360286182462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44860.14020340432367798No Hit
AAAAAGTACTCTGCGTTGATACCAC44650.13954707987187295No Hit
GAGTACATGGTAAGCAGTGGTATCA43440.1357654008876632No Hit
TGGGAAGCAGTGGTATCAACGCAGA34270.10710589982551146No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34050.1064183218283824No Hit
ATGGTAAGCAGTGGTATCAACGCAG32510.10160527584847906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC651.8838182E-716.1002434
TCTACGC500.00148385613.3192923
TAACGCA905.316688E-712.68503955
CTAGCAC2100.011.3259283
AAGACCG600.005814886711.099415
CCGCGTT600.005814886711.099414
CGATCTT600.005892595311.07963119
ATTAACG951.3396359E-511.0159553
ACCGAGC909.5764146E-510.5495578
TACGCAG1002.362457E-510.46515755
AAAAGTA8550.09.9032332
GTGTAAG3200.09.8153891
TATACTG5950.09.5937275
TAGGACC1701.6278136E-89.5137794
GCACCGT800.00453713449.4950476
TCGCCCA800.00453888959.49460112
ACTGTCC3200.09.4946018
GTGCGAT1002.7726687E-49.49460116
CTAGACA2357.2759576E-129.3113584
GTACAAA4650.09.2109221
Produced by FastQC (version 0.11.5)