##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294734_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3199637 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14820962502934 33.0 33.0 33.0 33.0 33.0 2 32.20459320854209 33.0 33.0 33.0 33.0 33.0 3 32.27714893908278 33.0 33.0 33.0 33.0 33.0 4 36.02983775972087 37.0 37.0 37.0 33.0 37.0 5 35.877049177766104 37.0 37.0 37.0 33.0 37.0 6 35.81908260218268 37.0 37.0 37.0 33.0 37.0 7 35.77558079244614 37.0 37.0 37.0 33.0 37.0 8 35.78643139831175 37.0 37.0 37.0 33.0 37.0 9 35.745627394607574 37.0 37.0 37.0 33.0 37.0 10 35.744054091135965 37.0 37.0 37.0 33.0 37.0 11 35.77385778449243 37.0 37.0 37.0 33.0 37.0 12 35.7482051870259 37.0 37.0 37.0 33.0 37.0 13 35.63716790373408 37.0 37.0 37.0 33.0 37.0 14 37.43630230554278 40.0 37.0 40.0 33.0 40.0 15 37.35974455852336 40.0 37.0 40.0 33.0 40.0 16 37.284925446230304 40.0 37.0 40.0 33.0 40.0 17 37.375728246672985 40.0 37.0 40.0 33.0 40.0 18 37.19664605703709 40.0 37.0 40.0 33.0 40.0 19 37.2053176657227 40.0 37.0 40.0 33.0 40.0 20 37.21262505715492 40.0 37.0 40.0 33.0 40.0 21 37.25141133197297 40.0 37.0 40.0 33.0 40.0 22 37.24423426782476 40.0 37.0 40.0 33.0 40.0 23 37.29869669590644 40.0 37.0 40.0 33.0 40.0 24 37.318570825378 40.0 37.0 40.0 33.0 40.0 25 36.30494740497125 40.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.215742979865162 1101 2 0.13820472901398517 1101 3 0.16191625170774415 1101 4 0.25354479022194454 1101 5 0.44111629906591077 1101 6 0.3593741126425769 1101 7 0.34968241665955446 1101 8 0.30360208981666403 1101 9 0.28932666775515514 1101 10 0.3213043244504732 1101 11 0.28634789699503926 1101 12 0.28808231875513 1101 13 0.3292043416058945 1101 14 0.6362517198095006 1101 15 0.5844114677744017 1101 16 0.6749320606281586 1101 17 0.6298651596721712 1101 18 0.6939140432067745 1101 19 0.6104067600288516 1101 20 0.6257395854395256 1101 21 0.5058492446800429 1101 22 0.5619379767966208 1101 23 0.5958197096701667 1101 24 0.536183730338827 1101 25 0.6003317159032022 1102 1 0.19028201800168176 1102 2 0.12838501301200722 1102 3 0.13149840511908195 1102 4 0.2373727868409503 1102 5 0.3438425303642276 1102 6 0.23096571734073734 1102 7 0.27546986437396015 1102 8 0.22887030804619002 1102 9 0.26195324323549585 1102 10 0.2700501721247264 1102 11 0.27475341666905706 1102 12 0.27042171004937643 1102 13 0.30971069032001 1102 14 0.5257615911676936 1102 15 0.5664887345326903 1102 16 0.5156002442310381 1102 17 0.5501714735894865 1102 18 0.6899123263378613 1102 19 0.5609922012696984 1102 20 0.5672760299809596 1102 21 0.6008067292033346 1102 22 0.558323576367151 1102 23 0.38528955194878023 1102 24 0.3800813801086278 1102 25 0.5573429942346166 1103 1 0.13795384255426768 1103 2 0.10227312146763978 1103 3 0.14277905343578112 1103 4 0.12483029588870664 1103 5 0.2763651666928695 1103 6 0.1812972885511286 1103 7 0.1800557504266891 1103 8 0.11621163040628346 1103 9 0.16722796828960895 1103 10 0.28722522455930033 1103 11 0.19086637723133038 1103 12 0.19186696202894638 1103 13 0.16182461048896357 1103 14 0.26070939247892255 1103 15 0.40235232784817043 1103 16 0.4517838904654141 1103 17 0.28503194667759146 1103 18 0.43364414236840787 1103 19 0.3885483209454961 1103 20 0.3813660372733665 1103 21 0.3753373023625528 1103 22 0.34099173464002064 1103 23 0.3782935073150284 1103 24 0.3000144485463494 1103 25 0.2944085049566709 1104 1 0.07404811746944517 1104 2 0.043489403280936756 1104 3 0.036350551705005785 1104 4 0.06489743899704337 1104 5 0.31098515887849487 1104 6 0.14170769687557083 1104 7 0.2568856894892093 1104 8 0.19433436078746524 1104 9 0.23015558142930104 1104 10 0.16774587259823193 1104 11 0.12463479507884756 1104 12 0.1286142706283826 1104 13 0.1443353260696938 1104 14 0.3180913570926194 1104 15 0.307636631373029 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13 0.20984942687820762 1107 14 0.21021766704064504 1107 15 0.19543198788185379 1107 16 0.2935179852564005 1107 17 0.24113041086624776 1107 18 0.39569469877120866 1107 19 0.2931800681453396 1107 20 0.312557800532133 1107 21 0.31846164499010143 1107 22 0.2764688662620074 1107 23 0.33688579659006024 1107 24 0.2710511162738882 1107 25 0.25632960803700655 1108 1 0.076601302157421 1108 2 0.017673632722114974 1108 3 0.07198534280504276 1108 4 -0.23627799700250307 1108 5 0.19472985759772143 1108 6 0.16103824098337327 1108 7 0.11868638084938965 1108 8 0.10364480052862035 1108 9 0.11520536525776492 1108 10 0.12133093713645593 1108 11 0.18738522118523804 1108 12 0.2306110211278991 1108 13 0.18890821128605495 1108 14 0.30712395161129535 1108 15 0.24507009303558647 1108 16 0.31470628887045393 1108 17 0.3071731377315814 1108 18 0.3854589931745167 1108 19 0.2900024102199694 1108 20 0.3140752003453997 1108 21 0.2368004505444503 1108 22 0.271531055379036 1108 23 0.28700137405933646 1108 24 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-1.9192359540813584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 4.0 8 40.0 9 94.0 10 141.0 11 261.0 12 535.0 13 1169.0 14 2150.0 15 3734.0 16 5385.0 17 6352.0 18 7090.0 19 7603.0 20 8233.0 21 9110.0 22 10652.0 23 11639.0 24 13161.0 25 14808.0 26 16140.0 27 18128.0 28 20967.0 29 25458.0 30 32846.0 31 46133.0 32 63900.0 33 95855.0 34 145460.0 35 247334.0 36 493981.0 37 1891273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01660201502987 19.255285685608563 11.14316767797544 25.58494462138613 2 12.5761856139558 21.94064421439392 36.61900739064136 28.864162781008922 3 21.179871341655318 27.379293338588095 27.78640201997914 23.654433299777445 4 13.17937003478832 18.946680514070817 34.17046996268639 33.70347948845448 5 12.19746001860845 38.6415330456571 35.46243814538079 13.698568790353672 6 33.268475983557806 32.31677642405642 18.10889095807277 16.305856634313006 7 24.56152797476211 32.607979537659624 22.959375978627556 19.871116508950713 8 26.676194902320997 35.831364605656994 21.30710807838323 16.185332413638776 9 26.314491622601555 16.533645703663026 21.16569431192007 35.98616836181535 10 16.425118357213133 30.616763906894413 32.72306599875736 20.235051737135098 11 29.709848245237897 26.177290166756467 20.170825681421263 23.942035906584376 12 23.313072682688084 30.058812976734213 29.207309336005306 17.420805004572397 13 29.222096131529916 20.929561697155023 23.874364498222768 25.973977673092293 14 21.42139873991956 24.811033251584476 26.895394696335867 26.872173312160097 15 30.831951924471312 24.029513394040315 22.158610740235126 22.979923941253244 16 19.375604170098047 28.08034161375181 31.730161890239422 20.81389232591072 17 24.97898980415591 28.938251432896916 28.245391586608104 17.837367176339068 18 25.283555603338755 25.362158269828733 29.26397588226414 20.090310244568368 19 23.072196543769515 30.222686306125148 29.66821299950963 17.036904150595714 20 23.185348838008814 26.583984370727055 28.7690134849672 21.46165330629693 21 19.22258681219151 29.377988815606273 30.619598410694714 20.77982596150751 22 21.80612988285859 28.512921934581954 23.787573402857888 25.893374779701574 23 17.852118849732015 29.435714113819788 28.245704122061344 24.46646291438685 24 20.711255683066547 33.72295044719135 25.744076593688597 19.8217172760535 25 21.97656504977473 26.38188802592963 23.498536331613114 28.143010592682533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5702.0 1 5702.0 2 6203.5 3 6705.0 4 6705.0 5 6705.0 6 9156.0 7 11607.0 8 11607.0 9 11607.0 10 12269.0 11 12931.0 12 12931.0 13 12931.0 14 16077.5 15 19224.0 16 19224.0 17 19224.0 18 29884.0 19 40544.0 20 40544.0 21 40544.0 22 58864.0 23 77184.0 24 77184.0 25 77184.0 26 101017.5 27 124851.0 28 124851.0 29 124851.0 30 148677.5 31 172504.0 32 172504.0 33 172504.0 34 192258.0 35 212012.0 36 212012.0 37 212012.0 38 228483.0 39 244954.0 40 244954.0 41 244954.0 42 281160.0 43 317366.0 44 317366.0 45 317366.0 46 555490.5 47 793615.0 48 793615.0 49 793615.0 50 682005.5 51 570396.0 52 570396.0 53 570396.0 54 415429.5 55 260463.0 56 260463.0 57 260463.0 58 200645.5 59 140828.0 60 140828.0 61 140828.0 62 117493.0 63 94158.0 64 94158.0 65 94158.0 66 73656.0 67 53154.0 68 53154.0 69 53154.0 70 39106.5 71 25059.0 72 25059.0 73 25059.0 74 17941.0 75 10823.0 76 10823.0 77 10823.0 78 7310.0 79 3797.0 80 3797.0 81 3797.0 82 2540.0 83 1283.0 84 1283.0 85 1283.0 86 822.0 87 361.0 88 361.0 89 361.0 90 229.5 91 98.0 92 98.0 93 98.0 94 57.0 95 16.0 96 16.0 97 16.0 98 9.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045973965171674167 2 0.001562677266202385 3 0.0 4 0.0 5 0.2020229169746443 6 0.00962609195980669 7 9.37606359721431E-5 8 7.500850877771448E-4 9 5.000567251847632E-4 10 3.12535453240477E-5 11 3.12535453240477E-5 12 9.37606359721431E-5 13 0.0 14 0.0 15 1.5626772662023848E-4 16 0.0 17 0.0 18 0.0 19 7.500850877771448E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.025784174892339348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3199637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.523970158721845 #Duplication Level Percentage of deduplicated Percentage of total 1 89.02691944592345 47.650741797465415 2 7.967813266636837 8.529379990274762 3 1.4674638803707434 2.3563347882589754 4 0.5011305678062927 1.072899902275494 5 0.24581946453972534 0.6578616842228622 6 0.15737684648928912 0.5054060181099876 7 0.11276245069436479 0.4224845832192668 8 0.08057413072853807 0.345011789494338 9 0.05871893927160066 0.2828583677992459 >10 0.33280432103656654 3.232913482539306 >50 0.02430760615416788 0.9044502657439134 >100 0.016173486501587644 1.7921317949143558 >500 0.002341178085408869 0.9381269213766188 >1k 0.003394708223840118 4.05481600018013 >5k 9.364712341627912E-4 3.928097126308284 >10k+ 0.0014632363033793613 23.32648548781716 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 123425 3.857468831620587 No Hit GTACATGGGAAGCAGTGGTATCAAC 116663 3.6461323581393765 No Hit GAGTACATGGGAAGCAGTGGTATCA 58030 1.8136432351544878 No Hit CATGTACTCTGCGTTGATACCACTG 47068 1.471041871312277 No Hit GCTTCCCATGTACTCTGCGTTGATA 40583 1.2683626298858277 No Hit GCGTTGATACCACTGCTTCCCATGT 38170 1.1929478250189005 No Hit ACGCAGAGTACATGGGAAGCAGTGG 34835 1.0887172513632015 No Hit GTATCAACGCAGAGTACATGGGAAG 32378 1.0119272905020162 No Hit TATCAACGCAGAGTACATGGGAAGC 25995 0.8124359106986199 No Hit ACTCTGCGTTGATACCACTGCTTCC 25855 0.8080604143532532 No Hit GGTATCAACGCAGAGTACATGGGAA 21267 0.6646691484065224 No Hit CAGTGGTATCAACGCAGAGTACATG 18405 0.5752215016890979 No Hit GTGGTATCAACGCAGAGTACATGGG 15890 0.4966188351991179 No Hit CATGGGAAGCAGTGGTATCAACGCA 14533 0.4542077741943852 No Hit ACATGGGAAGCAGTGGTATCAACGC 14337 0.44808207931087185 No Hit GCAGTGGTATCAACGCAGAGTACAT 14294 0.4467381768619378 No Hit ATACCACTGCTTCCCATGTACTCTG 13260 0.4144220109968725 No Hit GGGAAGCAGTGGTATCAACGCAGAG 12710 0.3972325610686462 No Hit CTGCTTCCCATGTACTCTGCGTTGA 11955 0.3736361343489902 No Hit GTACATGGTAAGCAGTGGTATCAAC 11940 0.37316733116912953 No Hit GTACTCTGCGTTGATACCACTGCTT 11087 0.3465080570077168 No Hit CTTCCCATGTACTCTGCGTTGATAC 11080 0.3462892821904485 No Hit CCATGTACTCTGCGTTGATACCACT 10443 0.3263807738190301 No Hit GATACCACTGCTTCCCATGTACTCT 10397 0.3249431107341239 No Hit TACCACTGCTTCCCATGTACTCTGC 10006 0.31272297451242126 No Hit AAGCAGTGGTATCAACGCAGAGTAC 9944 0.3107852547023303 No Hit GCAGAGTACATGGGAAGCAGTGGTA 9543 0.29825258302738716 No Hit CCACTGCTTCCCATGTACTCTGCGT 9540 0.298158822391415 No Hit ACCATGTACTCTGCGTTGATACCAC 9475 0.2961273419453519 No Hit ATGGGAAGCAGTGGTATCAACGCAG 9345 0.29206438105322574 No Hit GTATCAACGCAGAGTACTTTTTTTT 9113 0.2848135585380467 No Hit GTTGATACCACTGCTTCCCATGTAC 8728 0.2727809435882883 No Hit GTACATGGGTAAGCAGTGGTATCAA 7890 0.24659047260673633 No Hit CCCCATGTACTCTGCGTTGATACCA 7122 0.22258774979786766 No Hit TTCCCATGTACTCTGCGTTGATACC 7052 0.22040000162518433 No Hit TATCAACGCAGAGTACTTTTTTTTT 6854 0.2142117996510229 No Hit GGTATCAACGCAGAGTACTTTTTTT 6564 0.2051482715070491 No Hit GGTAAGCAGTGGTATCAACGCAGAG 6539 0.2043669328739479 No Hit TGATACCACTGCTTCCCATGTACTC 6312 0.19727237808538906 No Hit ATCAACGCAGAGTACATGGGAAGCA 5706 0.17833272961901614 No Hit GCTTACCATGTACTCTGCGTTGATA 5662 0.17695757362475806 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4870 0.15220476572811228 No Hit GGAAGCAGTGGTATCAACGCAGAGT 4561 0.14254742022298156 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4542 0.14195360286182462 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4486 0.14020340432367798 No Hit AAAAAGTACTCTGCGTTGATACCAC 4465 0.13954707987187295 No Hit GAGTACATGGTAAGCAGTGGTATCA 4344 0.1357654008876632 No Hit TGGGAAGCAGTGGTATCAACGCAGA 3427 0.10710589982551146 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3405 0.1064183218283824 No Hit ATGGTAAGCAGTGGTATCAACGCAG 3251 0.10160527584847906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 6.25070906480954E-5 0.0 11 0.0 0.0 0.0 9.37606359721431E-5 0.0 12 0.0 0.0 0.0 1.250141812961908E-4 0.0 13 0.0 0.0 0.0 1.875212719442862E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAACGC 65 1.8838182E-7 16.100243 4 TCTACGC 50 0.001483856 13.319292 3 TAACGCA 90 5.316688E-7 12.6850395 5 CTAGCAC 210 0.0 11.325928 3 AAGACCG 60 0.0058148867 11.09941 5 CCGCGTT 60 0.0058148867 11.09941 4 CGATCTT 60 0.0058925953 11.079631 19 ATTAACG 95 1.3396359E-5 11.015955 3 ACCGAGC 90 9.5764146E-5 10.549557 8 TACGCAG 100 2.362457E-5 10.4651575 5 AAAAGTA 855 0.0 9.903233 2 GTGTAAG 320 0.0 9.815389 1 TATACTG 595 0.0 9.593727 5 TAGGACC 170 1.6278136E-8 9.513779 4 GCACCGT 80 0.0045371344 9.495047 6 TCGCCCA 80 0.0045388895 9.494601 12 ACTGTCC 320 0.0 9.494601 8 GTGCGAT 100 2.7726687E-4 9.494601 16 CTAGACA 235 7.2759576E-12 9.311358 4 GTACAAA 465 0.0 9.210922 1 >>END_MODULE