FastQCFastQC Report
Wed 25 May 2016
SRR1294733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023235
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1618394.022608671877234No Hit
CCCATGTACTCTGCGTTGATACCAC1571783.9067566274403562No Hit
GAGTACATGGGAAGCAGTGGTATCA741051.8419257140087515No Hit
CATGTACTCTGCGTTGATACCACTG572761.423630486412054No Hit
GCGTTGATACCACTGCTTCCCATGT507681.2618701119869955No Hit
GCTTCCCATGTACTCTGCGTTGATA436501.0849478094120777No Hit
GTATCAACGCAGAGTACATGGGAAG422941.0512435888035374No Hit
ACGCAGAGTACATGGGAAGCAGTGG410381.0200249301867776No Hit
ACTCTGCGTTGATACCACTGCTTCC335540.8340054707219439No Hit
TATCAACGCAGAGTACATGGGAAGC325010.807832502948498No Hit
GGTATCAACGCAGAGTACATGGGAA275590.6849960293147181No Hit
CAGTGGTATCAACGCAGAGTACATG216440.5379750375009165No Hit
GTGGTATCAACGCAGAGTACATGGG210010.5219928738937696No Hit
ATACCACTGCTTCCCATGTACTCTG176530.4387762584089669No Hit
GCAGTGGTATCAACGCAGAGTACAT170710.42431028761680584No Hit
ACATGGGAAGCAGTGGTATCAACGC161750.40203965217045484No Hit
CATGGGAAGCAGTGGTATCAACGCA156470.38891588485385514No Hit
GTACTCTGCGTTGATACCACTGCTT154670.38444187326865076No Hit
GTACATGGTAAGCAGTGGTATCAAC151650.3769354760534744No Hit
CTGCTTCCCATGTACTCTGCGTTGA150580.3742759247222695No Hit
GGGAAGCAGTGGTATCAACGCAGAG147710.36714236180586024No Hit
CTTCCCATGTACTCTGCGTTGATAC137180.3409693940324142No Hit
TACCACTGCTTCCCATGTACTCTGC133010.3306046005266906No Hit
GATACCACTGCTTCCCATGTACTCT132640.3296849425897319No Hit
CCATGTACTCTGCGTTGATACCACT122150.3036113972959571No Hit
GTTGATACCACTGCTTCCCATGTAC117640.29240151271302817No Hit
GCAGAGTACATGGGAAGCAGTGGTA116800.2903136406399328No Hit
CCACTGCTTCCCATGTACTCTGCGT115500.28708241005061846No Hit
AAGCAGTGGTATCAACGCAGAGTAC115150.28621246335349537No Hit
ACCATGTACTCTGCGTTGATACCAC109760.2728152842177999No Hit
ATGGGAAGCAGTGGTATCAACGCAG103680.2577030673077759No Hit
TTCCCATGTACTCTGCGTTGATACC102340.2543724142387904No Hit
GTACATGGGTAAGCAGTGGTATCAA93340.23200235631276822No Hit
TGATACCACTGCTTCCCATGTACTC87840.21833176535797685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA83090.20652534589702068No Hit
CCCCATGTACTCTGCGTTGATACCA72400.17995468820488986No Hit
ATCAACGCAGAGTACATGGGAAGCA70260.1746355855424801No Hit
GGTAAGCAGTGGTATCAACGCAGAG69340.1723488685100423No Hit
GCTTACCATGTACTCTGCGTTGATA55530.1380232574035571No Hit
AAAAAGTACTCTGCGTTGATACCAC49480.12298560735328659No Hit
GGAAGCAGTGGTATCAACGCAGAGT48650.12092259090010898No Hit
GAGTACATGGTAAGCAGTGGTATCA47500.1180641946095617No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA46310.11510637583934322No Hit
TCCATGTACTCTGCGTTGATACCAC43110.10715257746564642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA8950.012.4209851
TCGGAAC701.0964426E-412.208652513
AAGCGGT2450.012.01531310
CTAGACT2700.011.6086424
AGCGGTG2500.011.39516711
GTCTAGA2200.011.2290731
ATACGAC1303.1688614E-810.98529219
TATCGAC1951.8189894E-1210.74117419
CGCAAAA2950.010.6259162
TCTGTCG909.5625895E-510.5512128
CGGTGGT2650.010.39143313
GTATCGA2200.010.3700718
GTACTAG1651.02118065E-89.7894481
GCTCTAG1856.475602E-109.7583221
GTGTAGG4100.09.7332691
TAGGACA3050.09.6536844
GGAACGA1201.6939981E-59.50376915
GTATTAG3600.09.5015241
TAATACG800.0045260149.497984
CGGTACT800.0045260149.497984
Produced by FastQC (version 0.11.5)