##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294733_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4023235
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94879021483955	33.0	33.0	33.0	27.0	33.0
2	30.994956546162477	33.0	33.0	33.0	27.0	33.0
3	30.952405216200397	33.0	33.0	33.0	27.0	33.0
4	34.2475649570557	37.0	37.0	37.0	27.0	37.0
5	34.72558500808429	37.0	37.0	37.0	27.0	37.0
6	34.7900532780213	37.0	37.0	37.0	27.0	37.0
7	34.84409958652676	37.0	37.0	37.0	33.0	37.0
8	34.72852294235857	37.0	37.0	37.0	27.0	37.0
9	34.419272799128066	37.0	37.0	37.0	27.0	37.0
10	34.55224539456432	37.0	37.0	37.0	27.0	37.0
11	34.8136377815365	37.0	37.0	37.0	33.0	37.0
12	34.75856195325404	37.0	37.0	37.0	27.0	37.0
13	34.60778428304586	37.0	37.0	37.0	27.0	37.0
14	35.847732483933946	40.0	37.0	40.0	27.0	40.0
15	36.07221850078357	40.0	37.0	40.0	27.0	40.0
16	36.039196566941776	40.0	37.0	40.0	27.0	40.0
17	36.05522123365898	40.0	37.0	40.0	27.0	40.0
18	36.03098228167134	40.0	37.0	40.0	27.0	40.0
19	36.01111816734543	40.0	37.0	40.0	27.0	40.0
20	35.95026539588167	40.0	37.0	40.0	27.0	40.0
21	35.87999358725006	40.0	37.0	40.0	27.0	40.0
22	35.79854420634141	40.0	37.0	40.0	27.0	40.0
23	35.4639261688666	37.0	37.0	40.0	27.0	40.0
24	35.80924405360363	37.0	37.0	40.0	27.0	40.0
25	34.68044397108297	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.37781182916985756
1101	2	0.5111092811060587
1101	3	0.27945571614113973
1101	4	0.32946654864367986
1101	5	0.3387018777514186
1101	6	0.3460127854105508
1101	7	0.33570848543611476
1101	8	0.4534935267062963
1101	9	0.7018174610927517
1101	10	0.686061406064681
1101	11	0.3997341836892545
1101	12	0.46789118846027833
1101	13	0.45650983833006364
1101	14	1.1503707859165644
1101	15	0.8836346055644739
1101	16	0.7635989962540464
1101	17	0.9059453141683562
1101	18	0.8990345578786219
1101	19	0.757058647071922
1101	20	0.893044663156978
1101	21	0.9437838481968157
1101	22	0.9479031204922492
1101	23	1.192993696726262
1101	24	0.9671763251997163
1101	25	0.784954816391803
1102	1	0.3154447945664849
1102	2	0.46612330202705365
1102	3	0.255429298551924
1102	4	0.19260904617445362
1102	5	0.2728288155492109
1102	6	0.243515471162695
1102	7	0.32059137669384086
1102	8	0.34362679824135967
1102	9	0.5430545500607522
1102	10	0.6471484820775757
1102	11	0.3415073989679982
1102	12	0.4094889282588454
1102	13	0.3747319442903958
1102	14	0.882356817518243
1102	15	0.7368974853608492
1102	16	0.5425223925897171
1102	17	0.6654895987575742
1102	18	0.6921092016717338
1102	19	0.7065115846329917
1102	20	0.8145901524027792
1102	21	0.8111168359631264
1102	22	0.840231225405347
1102	23	1.1202426218767698
1102	24	0.9222641119512147
1102	25	0.757936654518673
1103	1	0.24523197363738447
1103	2	0.44998509310881474
1103	3	0.15820372939278826
1103	4	-0.05213457682940259
1103	5	0.16923975056186435
1103	6	0.10840646553475608
1103	7	0.04757944069533693
1103	8	0.234755440548156
1103	9	0.44031565255340865
1103	10	0.44177222318982245
1103	11	0.22195623980507406
1103	12	0.2587944289102637
1103	13	0.36640172845815755
1103	14	0.9149285609251621
1103	15	0.5600733692977826
1103	16	0.46690099824665054
1103	17	0.42618357591214817
1103	18	0.511821390291999
1103	19	0.5732106003130966
1103	20	0.703102335459107
1103	21	0.780397343725383
1103	22	0.6203025087670966
1103	23	1.0041341208256114
1103	24	0.7349648185972484
1103	25	0.7199766276795643
1104	1	0.24540875164839804
1104	2	0.5551079461971611
1104	3	0.24179001591349092
1104	4	0.2091402162380902
1104	5	0.2703614059320216
1104	6	0.22103500446921487
1104	7	0.25723533197653126
1104	8	0.37946006105428864
1104	9	0.5386352710258464
1104	10	0.6425530926130492
1104	11	0.428310527075503
1104	12	0.39497231242193465
1104	13	0.3897330967590804
1104	14	0.8665283949890892
1104	15	0.707965820019453
1104	16	0.6656462849639624
1104	17	0.6835374870715398
1104	18	0.6072050844402952
1104	19	0.6219819667807656
1104	20	0.7625025491962631
1104	21	0.8226634624911853
1104	22	0.8308315535857318
1104	23	1.1567040034177651
1104	24	0.9333672858101565
1104	25	0.7102692621482802
1105	1	0.1094492510174696
1105	2	0.266545219799486
1105	3	0.23495062576218473
1105	4	0.13803105060591037
1105	5	0.19433106290188817
1105	6	0.09344488434707898
1105	7	0.19804061450862775
1105	8	0.2586088092131007
1105	9	-1.5503312514248222
1105	10	-0.18159182395120865
1105	11	0.025440077713263065
1105	12	0.17383306801505682
1105	13	0.1850855801228235
1105	14	-1.7428219184887581
1105	15	-0.38420108078429394
1105	16	-0.0786493035943181
1105	17	0.05202798292384614
1105	18	0.04351449015680231
1105	19	0.2321843279775493
1105	20	0.23594440084241342
1105	21	0.4639012074136204
1105	22	0.48718693625350085
1105	23	0.854374993834945
1105	24	0.6465655617468116
1105	25	0.26148488216065147
1106	1	0.07538491021931648
1106	2	0.03508861898105664
1106	3	0.11540122013280296
1106	4	-1.1291682463601234
1106	5	-0.327214310270584
1106	6	-0.07145292768483102
1106	7	-0.03582393665221417
1106	8	0.18176184310841137
1106	9	0.2927058592380263
1106	10	0.33984454027081057
1106	11	0.224464466731078
1106	12	0.0758509329701269
1106	13	0.11177331802836932
1106	14	0.5615424365242703
1106	15	0.3481677391697815
1106	16	0.1588403005991026
1106	17	-3.9917512347642514E-4
1106	18	0.21386955252562956
1106	19	0.11625325452816071
1106	20	0.36858209314861057
1106	21	0.4971611222626038
1106	22	0.47914923369116735
1106	23	0.7106469658661467
1106	24	0.41454432734967384
1106	25	0.27934502881348067
1107	1	0.25872818123606933
1107	2	0.2469592972308945
1107	3	0.20447317166683376
1107	4	0.3518711216700652
1107	5	0.3978914107310345
1107	6	0.16795218536233136
1107	7	0.26432402865685134
1107	8	0.25849814168856966
1107	9	0.4436329577104914
1107	10	0.3588227936014121
1107	11	0.1677571766992969
1107	12	0.3115265255962285
1107	13	0.2655636408991313
1107	14	0.630894604943343
1107	15	0.4457276471006324
1107	16	0.5292728223915688
1107	17	0.4902014764179867
1107	18	0.5934800176209905
1107	19	0.4304195456067461
1107	20	0.6109395720759636
1107	21	0.5331143651358019
1107	22	0.5948546861668049
1107	23	0.7905278575649461
1107	24	0.6710406003920752
1107	25	0.5578093604890668
1108	1	0.5092032405354523
1108	2	0.40094313939322745
1108	3	0.46729127611297017
1108	4	0.7085684948898319
1108	5	0.6364280107330984
1108	6	0.5154554371120383
1108	7	0.4258339762430907
1108	8	0.48613873922317197
1108	9	0.3215841901743204
1108	10	0.5921887262569143
1108	11	0.34036941720113134
1108	12	0.45327989165534177
1108	13	0.5069729554691733
1108	14	-1.055895209561534
1108	15	0.0014983123131599996
1108	16	0.3354491146335761
1108	17	0.5056985523436026
1108	18	0.6262605000518491
1108	19	0.5561790788565162
1108	20	0.5880127872797942
1108	21	0.6578142962841582
1108	22	0.7779232623587049
1108	23	1.0914659197546754
1108	24	0.9507614802351227
1108	25	0.8121131960413521
1109	1	0.4195993095196435
1109	2	0.32365724252966643
1109	3	0.4466166098271316
1109	4	0.5313086736911785
1109	5	0.5612590030664464
1109	6	0.30675353668708993
1109	7	0.2949186704275135
1109	8	0.4055396211478879
1109	9	0.6051612316446224
1109	10	0.4733759524046448
1109	11	0.39732366709096567
1109	12	0.3552492852968072
1109	13	0.2823995587196251
1109	14	0.7718667149949567
1109	15	0.49449662770275893
1109	16	0.4177237972120196
1109	17	0.48124642555264785
1109	18	0.5858514906909704
1109	19	0.4867596116172095
1109	20	0.6130718635915358
1109	21	0.6239984444539104
1109	22	0.7130608267966423
1109	23	1.1313546402616552
1109	24	0.8945869264607182
1109	25	0.7548407655657314
1110	1	0.33454743238813833
1110	2	0.2539164559568654
1110	3	0.44224697219355846
1110	4	0.5662586880066627
1110	5	0.456995703768051
1110	6	0.31409165962843133
1110	7	0.2779064134312321
1110	8	0.33888628844174207
1110	9	0.48281942766594454
1110	10	0.45032491753497794
1110	11	0.24717361682316863
1110	12	0.1830391109663836
1110	13	0.1924963669769042
1110	14	0.606778208980046
1110	15	0.5262306635876541
1110	16	0.4641584751888459
1110	17	0.49096678400407257
1110	18	0.481057573841305
1110	19	0.48420297284198455
1110	20	0.6455035866145522
1110	21	0.6085364085235767
1110	22	0.7540234942998936
1110	23	0.9095613188720932
1110	24	0.8093896597744674
1110	25	0.7623515632958515
1111	1	0.13347835491849125
1111	2	0.016873225540688708
1111	3	0.2951457811552949
1111	4	0.4682440814137365
1111	5	0.25107057754109974
1111	6	0.21537753618741817
1111	7	0.12459977809110967
1111	8	0.21774162977096978
1111	9	0.24166438309230642
1111	10	0.04763934399061753
1111	11	0.0951520982744043
1111	12	0.09709694664890378
1111	13	0.11093251435686824
1111	14	-1.8760915032935443
1111	15	-0.6435582741715749
1111	16	-0.10628801130194176
1111	17	0.11618257911476348
1111	18	0.20008747359177192
1111	19	0.1923117074079954
1111	20	0.27165370294945035
1111	21	0.23330490551869332
1111	22	0.03841404428602857
1111	23	-2.043884221285907
1111	24	-0.36948730478260927
1111	25	-0.017103910039985237
1112	1	-0.10914430383048312
1112	2	-0.2566394811253403
1112	3	0.03266750422266895
1112	4	0.2626746138810603
1112	5	-0.5629404771671744
1112	6	-0.8147968162900909
1112	7	-0.8156088400099648
1112	8	-0.6689032090094074
1112	9	-0.5151375632759496
1112	10	-0.8271107950033283
1112	11	-0.705081581129761
1112	12	-0.7008356874812733
1112	13	-0.8844161273002982
1112	14	-0.4157221713692536
1112	15	-0.7836698433113511
1112	16	-0.7176151442333492
1112	17	-0.7688465733200474
1112	18	-0.8736446733656251
1112	19	-0.7636367051922477
1112	20	-0.8939315228444329
1112	21	-1.8179726251264654
1112	22	-1.7197543691109516
1112	23	-1.2821290845491973
1112	24	-1.5540575419939202
1112	25	-1.7927450388694623
1113	1	0.24279818544083298
1113	2	-0.031842864054169695
1113	3	0.08239456714791871
1113	4	0.41060255102079424
1113	5	0.23416510651367872
1113	6	0.08573958181773378
1113	7	0.13227737547197194
1113	8	0.18719939793088258
1113	9	0.2743424917320212
1113	10	0.08233125476014891
1113	11	-0.08829017967193664
1113	12	0.040482047264646326
1113	13	-0.04168622318700699
1113	14	0.12310268304264582
1113	15	0.04039205563685755
1113	16	0.165799625901343
1113	17	0.20379352228508907
1113	18	0.2200333312796161
1113	19	-0.013372143133381087
1113	20	-0.5379783440566186
1113	21	-0.39951247467941187
1113	22	0.10661783392253454
1113	23	-0.11075920269468043
1113	24	0.12665743166810017
1113	25	-0.9726233908612727
1114	1	-0.9282868112088103
1114	2	-0.7096027397026745
1114	3	-0.44779375210815786
1114	4	-1.5666974906429942
1114	5	-0.7565984911960015
1114	6	-0.577877791443818
1114	7	-0.5391349955823657
1114	8	-0.39644075201562856
1114	9	-0.41613047946783865
1114	10	-0.4112240461034702
1114	11	-0.4884512988354075
1114	12	-0.5879252437908988
1114	13	-0.6062089975353402
1114	14	-0.5298595756380919
1114	15	-1.2378230751133898
1114	16	-0.8036648865424354
1114	17	-0.891222792737743
1114	18	-0.7933187858089354
1114	19	-0.6855900426024988
1114	20	-1.9696147900957186
1114	21	-6.638897869058287
1114	22	-7.457471069920743
1114	23	-7.716547216320425
1114	24	-5.711501747738556
1114	25	-7.151123549212436
1115	1	-1.017871179688285
1115	2	-1.0180685619234282
1115	3	-0.803735012154867
1115	4	-0.6497743780351044
1115	5	-0.7259011710119054
1115	6	-1.006646579931072
1115	7	-1.0884189256947039
1115	8	-0.7747930574161828
1115	9	-2.754699858927225
1115	10	-1.6333413588849908
1115	11	-1.120570346400001
1115	12	-1.0099547616249396
1115	13	-1.353126927510587
1115	14	-1.0358472571304418
1115	15	-2.0430129666301937
1115	16	-1.3419716989955646
1115	17	-3.1420377468773495
1115	18	-2.759896869725445
1115	19	-2.1217121950799296
1115	20	-1.5968652171552264
1115	21	-1.2161207472821758
1115	22	-0.7143983100601616
1115	23	-0.14609449708320454
1115	24	-1.2169635189670842
1115	25	-0.9427155738863249
1116	1	-1.324977559884939
1116	2	-0.699262370057113
1116	3	-0.4738057172800225
1116	4	-0.15206622611701448
1116	5	-0.18043051050810277
1116	6	-0.2532197796875124
1116	7	-0.6120892125494279
1116	8	-1.6747989233807488
1116	9	-0.764347512212602
1116	10	-2.214983529709315
1116	11	-0.9542783517404558
1116	12	-0.6474274296133515
1116	13	-0.6330387722130126
1116	14	-0.9068186951723689
1116	15	-0.9493443294886532
1116	16	-0.48722627661609863
1116	17	-0.3914466473039582
1116	18	-0.9698229681022923
1116	19	-0.6391167645090761
1116	20	-0.871401055434923
1116	21	-0.2956415928045004
1116	22	-0.011326336342733612
1116	23	0.14834790414820276
1116	24	0.02875149160261259
1116	25	0.07450231872564217
1201	1	0.19940888426280168
1201	2	0.33486313532170797
1201	3	0.09873775478769531
1201	4	0.16841068297715367
1201	5	0.2773981739448885
1201	6	0.13257232353510773
1201	7	0.14168624304752342
1201	8	0.3110202293138329
1201	9	0.5082442192831209
1201	10	0.44103144280950346
1201	11	0.27345859808851003
1201	12	0.34112148822007526
1201	13	0.26321795209384646
1201	14	0.9670108468552527
1201	15	0.6681166990419669
1201	16	0.6097722539114301
1201	17	0.6383604317743519
1201	18	0.5367651529041595
1201	19	0.4822145074751347
1201	20	0.6181655023247714
1201	21	0.6232137452847013
1201	22	0.6422415661740573
1201	23	0.5659092680755933
1201	24	0.5390389459448954
1201	25	0.4859995539234063
1202	1	-0.2562272117163573
1202	2	-0.006069452682933019
1202	3	-0.14070587181529248
1202	4	-0.26508071611967665
1202	5	-0.10641286171374986
1202	6	-0.13331351182775109
1202	7	-0.06016226708655381
1202	8	-0.03787376260423514
1202	9	0.07901562091262093
1202	10	0.1839943504959365
1202	11	0.022224034374886514
1202	12	-0.07747102547782703
1202	13	0.08184664679627218
1202	14	0.3180559125576963
1202	15	0.2635499778776591
1202	16	0.25534550800836797
1202	17	0.05774157830515492
1202	18	0.0323543492562095
1202	19	0.14877511857230274
1202	20	0.19282093478953755
1202	21	0.2151849542503541
1202	22	0.12714458977537646
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2211	5	0.4170112165206916
2211	6	0.26851883713688096
2211	7	0.2704763357537132
2211	8	0.33151711478848966
2211	9	0.49431332043788956
2211	10	0.4244320811279323
2211	11	0.2799600541368079
2211	12	0.4264518786300897
2211	13	0.258471871710114
2211	14	0.6633578816339494
2211	15	0.3662047236868915
2211	16	0.3478199161164426
2211	17	0.42226313445483754
2211	18	0.4323909454075121
2211	19	0.5397124883255842
2211	20	0.518516515804265
2211	21	0.5100106650797613
2211	22	0.6055919980562763
2211	23	0.7907886842587928
2211	24	0.6690564555165892
2211	25	0.628817546857924
2212	1	-0.16817163135010915
2212	2	-0.2040487594611058
2212	3	-0.09893403537180134
2212	4	-0.3784534598626905
2212	5	-0.168660026954079
2212	6	-0.4636391273810787
2212	7	-0.3156376565317558
2212	8	-0.13901869344856266
2212	9	-0.1528948466642106
2212	10	-0.47267371659659574
2212	11	-0.403092577653112
2212	12	-0.48773372111015334
2212	13	-0.45680466591000624
2212	14	-0.37478300428458056
2212	15	-0.6008526302225334
2212	16	-0.4280687633235942
2212	17	-0.3012546140802783
2212	18	-0.272269040943236
2212	19	-0.4905721616793741
2212	20	-0.4611342758422339
2212	21	-0.3782231199214934
2212	22	-0.4225405900801036
2212	23	0.07265136598573463
2212	24	-0.19355381830020235
2212	25	-0.8635382215537817
2213	1	-0.015879809508028586
2213	2	-1.0822439084495166
2213	3	-0.10628306476820981
2213	4	0.31228505647339944
2213	5	0.18403402322400098
2213	6	0.08392121492403959
2213	7	0.10027252542585074
2213	8	0.22716823406217657
2213	9	0.2330102808342076
2213	10	-0.21638119804559608
2213	11	0.11183980028296503
2213	12	0.07208751681545778
2213	13	-0.24627443195502963
2213	14	0.05156970792533144
2213	15	0.024847581279786368
2213	16	0.02980231447904913
2213	17	0.1347273456888871
2213	18	0.08704647769143037
2213	19	0.1676490572663809
2213	20	0.2652179189129029
2213	21	-0.3808352413874587
2213	22	-0.7596651095183873
2213	23	-0.022016740962598647
2213	24	0.07896525210499306
2213	25	-0.5282262583215811
2214	1	-0.14934273714941781
2214	2	-0.3949225335706821
2214	3	-0.3310228130475501
2214	4	-0.35498698451073807
2214	5	-0.22513804847659458
2214	6	-0.18685966710930302
2214	7	-0.4388540535802292
2214	8	-0.07369119558467219
2214	9	-0.04522320883064168
2214	10	-1.9341669716162002
2214	11	-0.8477301763705114
2214	12	-0.4764591180937856
2214	13	-0.249992748791513
2214	14	-0.707764331826553
2214	15	-0.4342883594739675
2214	16	-0.6492343826339635
2214	17	-0.4597085279391777
2214	18	-0.3715006397233864
2214	19	-1.2887006219331454
2214	20	-2.389466379790214
2214	21	-1.8623480686076448
2214	22	-1.936550362022409
2214	23	-2.3999090408966524
2214	24	-1.5604809419273735
2214	25	-1.925225406683893
2215	1	-0.3067827861998502
2215	2	-0.45058833194343606
2215	3	-0.8562568722358321
2215	4	-0.2828687104629921
2215	5	-0.28886157957771275
2215	6	-0.19077564168842542
2215	7	-0.16720210338645813
2215	8	-0.1896596812026985
2215	9	-0.1446090455672504
2215	10	-0.21567725263304283
2215	11	-0.4461584355987682
2215	12	-0.42779888841154445
2215	13	-0.2807346197335434
2215	14	-0.08703660352271925
2215	15	-0.6086461774686001
2215	16	-0.4541863154180561
2215	17	-0.22963502496874355
2215	18	-0.3025278768860744
2215	19	-2.0368921988198636
2215	20	-2.3024563134297793
2215	21	-3.24622246381697
2215	22	-2.361215144639118
2215	23	-3.444294684004838
2215	24	-2.357322105120943
2215	25	-1.6231218819242414
2216	1	-1.4603501555584373
2216	2	-1.2054312594628769
2216	3	-0.8239191823797256
2216	4	-0.8167332925616648
2216	5	-1.8902590258284988
2216	6	-1.513820020577107
2216	7	-1.143623153492264
2216	8	-5.081770468746807
2216	9	-3.925433812928354
2216	10	-2.0110883271019944
2216	11	-1.0511711638244137
2216	12	-1.1109998386438136
2216	13	-1.8577307799552685
2216	14	-3.329055326112197
2216	15	-1.9133587230114983
2216	16	-2.921674966544515
2216	17	-2.643556988301576
2216	18	-2.5727278867921086
2216	19	-1.6110763667287173
2216	20	-0.9363257551799933
2216	21	-0.737210144343571
2216	22	-0.6038327340848326
2216	23	-0.9857162109727042
2216	24	-0.6706456404133334
2216	25	-0.35477528522784496
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	89.0
8	551.0
9	1299.0
10	2245.0
11	3902.0
12	6416.0
13	9258.0
14	13278.0
15	18467.0
16	22733.0
17	25024.0
18	25817.0
19	26182.0
20	26767.0
21	28369.0
22	29527.0
23	30343.0
24	32324.0
25	34178.0
26	35809.0
27	39315.0
28	44779.0
29	52487.0
30	62467.0
31	76634.0
32	102216.0
33	146348.0
34	213929.0
35	341695.0
36	602428.0
37	1968355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.04114323365055	19.33226004960311	11.573835911907855	24.05276080483848
2	12.119053164945894	22.564034616490837	37.701304121826425	27.61560809673685
3	21.144231113444626	29.223794693109635	26.857978433548517	22.77399575989722
4	13.022356436583838	19.615798545204495	33.392451958658725	33.96939305955294
5	11.750064636741513	40.06829120343669	34.771732861319386	13.409911298502417
6	32.40527537103716	33.2091840476081	18.31507303910541	16.07046754224933
7	24.166584020415737	33.70599957098517	22.92587115983884	19.201545248760244
8	26.641240556865608	36.40900774291658	21.344634231640246	15.60511746857757
9	26.663397281057634	18.0802161075487	20.517789152963996	34.73859745842967
10	16.45209462512695	32.11720415728567	31.68600532610592	19.744695891481463
11	28.83260401895513	28.29767957476477	19.221934860067336	23.647781546212766
12	23.286660636525404	31.52394296669528	28.596789111344595	16.592607285434724
13	28.279544742186864	22.68419565179026	23.0003179119128	26.035941694110075
14	21.036576044931323	26.752414181025237	25.872270554311523	26.33873921973192
15	31.23665845750641	24.921479398719608	21.323749461864193	22.518112681909788
16	19.024717012085087	29.504068961060266	31.191283425384153	20.279930601470493
17	25.360539997196213	30.09026300901884	27.449956899251625	17.099240094533315
18	25.65091521728161	26.479570823110187	28.582976046339887	19.286537913268308
19	22.735951903130182	31.294731028476235	29.288300348925013	16.681016719468563
20	23.433820961594147	27.901690563392712	27.451627616222353	21.212860858790794
21	19.30924924009058	30.478269662803953	30.00668924275732	20.205791854348146
22	21.364889501257558	29.531470805014116	22.915998667691852	26.187641026036474
23	17.724541186385615	31.00751186181637	27.43812129326012	23.829825658537896
24	20.63917100403164	35.24622313692154	24.374897469166182	19.739708389880644
25	22.028002515154203	26.85467710063706	22.169654325736385	28.947666058472354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11242.0
1	11242.0
2	13463.0
3	15684.0
4	15684.0
5	15684.0
6	18742.5
7	21801.0
8	21801.0
9	21801.0
10	22451.0
11	23101.0
12	23101.0
13	23101.0
14	27682.5
15	32264.0
16	32264.0
17	32264.0
18	44104.5
19	55945.0
20	55945.0
21	55945.0
22	75872.5
23	95800.0
24	95800.0
25	95800.0
26	123209.5
27	150619.0
28	150619.0
29	150619.0
30	180491.5
31	210364.0
32	210364.0
33	210364.0
34	238336.5
35	266309.0
36	266309.0
37	266309.0
38	293821.0
39	321333.0
40	321333.0
41	321333.0
42	369094.0
43	416855.0
44	416855.0
45	416855.0
46	715294.0
47	1013733.0
48	1013733.0
49	1013733.0
50	865169.0
51	716605.0
52	716605.0
53	716605.0
54	513790.5
55	310976.0
56	310976.0
57	310976.0
58	235861.5
59	160747.0
60	160747.0
61	160747.0
62	130945.5
63	101144.0
64	101144.0
65	101144.0
66	77998.0
67	54852.0
68	54852.0
69	54852.0
70	40637.5
71	26423.0
72	26423.0
73	26423.0
74	18738.0
75	11053.0
76	11053.0
77	11053.0
78	7633.5
79	4214.0
80	4214.0
81	4214.0
82	2852.5
83	1491.0
84	1491.0
85	1491.0
86	985.5
87	480.0
88	480.0
89	480.0
90	316.0
91	152.0
92	152.0
93	152.0
94	91.0
95	30.0
96	30.0
97	30.0
98	24.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03256086209232123
2	0.013123767316599701
3	0.0171752333632015
4	0.020232474613091207
5	0.01876599303794086
6	0.009693691767942962
7	0.0015907596747393577
8	0.0018393158739173825
9	0.0013173478556435306
10	8.202354572874813E-4
11	0.0012427809958901232
12	0.002783829430793876
13	0.002684406951122666
14	6.95957357698469E-4
15	0.0021624389328488143
16	0.002361283892191234
17	0.002560128851533654
18	0.002286717032437827
19	5.219680182738518E-4
20	4.971123983560493E-4
21	0.08321661548480265
22	0.07814606902157094
23	0.07936399439754327
24	0.0013670590954791356
25	0.22785146778649518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4023235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.75089031111422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91996783987744	46.90607469989179
2	7.965170582245781	8.40339679386722
3	1.562730233125056	2.4730623334032535
4	0.5327919077511831	1.1242098993772778
5	0.2522175462473907	0.6652350058317241
6	0.14951274499923414	0.4732158246940915
7	0.10243839391202601	0.37826015366299964
8	0.07327013879740972	0.3092052043825819
9	0.057480107411186934	0.27289141570067266
>10	0.3222937099786002	3.2173533610140264
>50	0.03134344421498986	1.1515596681066413
>100	0.02234446977687652	2.38944422295142
>500	0.0031467016372248536	1.150923403183362
>1k	0.0034327654219885105	4.044202287071115
>5k	3.337410826933274E-4	1.3374875786771423
>10k+	0.0015256735208837827	25.703478148184672
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	161839	4.022608671877234	No Hit
CCCATGTACTCTGCGTTGATACCAC	157178	3.9067566274403562	No Hit
GAGTACATGGGAAGCAGTGGTATCA	74105	1.8419257140087515	No Hit
CATGTACTCTGCGTTGATACCACTG	57276	1.423630486412054	No Hit
GCGTTGATACCACTGCTTCCCATGT	50768	1.2618701119869955	No Hit
GCTTCCCATGTACTCTGCGTTGATA	43650	1.0849478094120777	No Hit
GTATCAACGCAGAGTACATGGGAAG	42294	1.0512435888035374	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	41038	1.0200249301867776	No Hit
ACTCTGCGTTGATACCACTGCTTCC	33554	0.8340054707219439	No Hit
TATCAACGCAGAGTACATGGGAAGC	32501	0.807832502948498	No Hit
GGTATCAACGCAGAGTACATGGGAA	27559	0.6849960293147181	No Hit
CAGTGGTATCAACGCAGAGTACATG	21644	0.5379750375009165	No Hit
GTGGTATCAACGCAGAGTACATGGG	21001	0.5219928738937696	No Hit
ATACCACTGCTTCCCATGTACTCTG	17653	0.4387762584089669	No Hit
GCAGTGGTATCAACGCAGAGTACAT	17071	0.42431028761680584	No Hit
ACATGGGAAGCAGTGGTATCAACGC	16175	0.40203965217045484	No Hit
CATGGGAAGCAGTGGTATCAACGCA	15647	0.38891588485385514	No Hit
GTACTCTGCGTTGATACCACTGCTT	15467	0.38444187326865076	No Hit
GTACATGGTAAGCAGTGGTATCAAC	15165	0.3769354760534744	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	15058	0.3742759247222695	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	14771	0.36714236180586024	No Hit
CTTCCCATGTACTCTGCGTTGATAC	13718	0.3409693940324142	No Hit
TACCACTGCTTCCCATGTACTCTGC	13301	0.3306046005266906	No Hit
GATACCACTGCTTCCCATGTACTCT	13264	0.3296849425897319	No Hit
CCATGTACTCTGCGTTGATACCACT	12215	0.3036113972959571	No Hit
GTTGATACCACTGCTTCCCATGTAC	11764	0.29240151271302817	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11680	0.2903136406399328	No Hit
CCACTGCTTCCCATGTACTCTGCGT	11550	0.28708241005061846	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11515	0.28621246335349537	No Hit
ACCATGTACTCTGCGTTGATACCAC	10976	0.2728152842177999	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10368	0.2577030673077759	No Hit
TTCCCATGTACTCTGCGTTGATACC	10234	0.2543724142387904	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9334	0.23200235631276822	No Hit
TGATACCACTGCTTCCCATGTACTC	8784	0.21833176535797685	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8309	0.20652534589702068	No Hit
CCCCATGTACTCTGCGTTGATACCA	7240	0.17995468820488986	No Hit
ATCAACGCAGAGTACATGGGAAGCA	7026	0.1746355855424801	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6934	0.1723488685100423	No Hit
GCTTACCATGTACTCTGCGTTGATA	5553	0.1380232574035571	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4948	0.12298560735328659	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4865	0.12092259090010898	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4750	0.1180641946095617	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4631	0.11510637583934322	No Hit
TCCATGTACTCTGCGTTGATACCAC	4311	0.10715257746564642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.4855619917802464E-5	0.0
7	0.0	0.0	0.0	2.4855619917802464E-5	0.0
8	0.0	0.0	0.0	2.4855619917802464E-5	0.0
9	0.0	0.0	0.0	2.4855619917802464E-5	0.0
10	0.0	0.0	0.0	1.2427809958901232E-4	0.0
11	0.0	0.0	0.0	1.988449593424197E-4	0.0
12	0.0	0.0	0.0	2.734118190958271E-4	0.0
13	0.0	0.0	0.0	2.734118190958271E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	895	0.0	12.420985	1
TCGGAAC	70	1.0964426E-4	12.2086525	13
AAGCGGT	245	0.0	12.015313	10
CTAGACT	270	0.0	11.608642	4
AGCGGTG	250	0.0	11.395167	11
GTCTAGA	220	0.0	11.229073	1
ATACGAC	130	3.1688614E-8	10.985292	19
TATCGAC	195	1.8189894E-12	10.741174	19
CGCAAAA	295	0.0	10.625916	2
TCTGTCG	90	9.5625895E-5	10.551212	8
CGGTGGT	265	0.0	10.391433	13
GTATCGA	220	0.0	10.37007	18
GTACTAG	165	1.02118065E-8	9.789448	1
GCTCTAG	185	6.475602E-10	9.758322	1
GTGTAGG	410	0.0	9.733269	1
TAGGACA	305	0.0	9.653684	4
GGAACGA	120	1.6939981E-5	9.503769	15
GTATTAG	360	0.0	9.501524	1
TAATACG	80	0.004526014	9.49798	4
CGGTACT	80	0.004526014	9.49798	4
>>END_MODULE