FastQCFastQC Report
Wed 25 May 2016
SRR1294733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023235
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1727104.292814116003664No Hit
GTACATGGGAAGCAGTGGTATCAAC1586183.942548720121991No Hit
GAGTACATGGGAAGCAGTGGTATCA795151.976394617764063No Hit
CATGTACTCTGCGTTGATACCACTG654081.6257563875836236No Hit
GCGTTGATACCACTGCTTCCCATGT548931.3643995441479306No Hit
ACGCAGAGTACATGGGAAGCAGTGG490551.2192924350678No Hit
GCTTCCCATGTACTCTGCGTTGATA488051.2130785300883493No Hit
GTATCAACGCAGAGTACATGGGAAG442571.1000351707021838No Hit
ACTCTGCGTTGATACCACTGCTTCC384390.9554251740204088No Hit
TATCAACGCAGAGTACATGGGAAGC371010.9221683545703894No Hit
GGTATCAACGCAGAGTACATGGGAA293430.7293384552480777No Hit
CAGTGGTATCAACGCAGAGTACATG260050.6463703959624532No Hit
GTGGTATCAACGCAGAGTACATGGG224690.5584809239331036No Hit
ACATGGGAAGCAGTGGTATCAACGC200100.4973609545552273No Hit
CATGGGAAGCAGTGGTATCAACGCA197580.4910973383359411No Hit
ATACCACTGCTTCCCATGTACTCTG195360.48557939071418893No Hit
GCAGTGGTATCAACGCAGAGTACAT192630.4787938064766289No Hit
GGGAAGCAGTGGTATCAACGCAGAG170520.42383803083836763No Hit
CTGCTTCCCATGTACTCTGCGTTGA161980.4026113314285643No Hit
GTACTCTGCGTTGATACCACTGCTT159530.3965217045487027No Hit
CTTCCCATGTACTCTGCGTTGATAC159090.39542805727231944No Hit
GATACCACTGCTTCCCATGTACTCT151540.37666206423437854No Hit
TACCACTGCTTCCCATGTACTCTGC146510.3641596874157239No Hit
CCATGTACTCTGCGTTGATACCACT146220.36343887443810763No Hit
GTACATGGTAAGCAGTGGTATCAAC145030.3604810556678891No Hit
AAGCAGTGGTATCAACGCAGAGTAC138710.344772303879838No Hit
GCAGAGTACATGGGAAGCAGTGGTA131910.3278704823357323No Hit
CCACTGCTTCCCATGTACTCTGCGT131460.3267519794394312No Hit
GTTGATACCACTGCTTCCCATGTAC125530.31201259682817434No Hit
ATGGGAAGCAGTGGTATCAACGCAG124920.3104964040131884No Hit
ACCATGTACTCTGCGTTGATACCAC123060.30587325870847715No Hit
TTCCCATGTACTCTGCGTTGATACC105160.2613816990556107No Hit
GTACATGGGTAAGCAGTGGTATCAA102350.2543972698587082No Hit
GTATCAACGCAGAGTACTTTTTTTT96220.23916077484909531No Hit
TGATACCACTGCTTCCCATGTACTC93480.23235033499161745No Hit
GGTAAGCAGTGGTATCAACGCAGAG83690.20801668309208882No Hit
ATCAACGCAGAGTACATGGGAAGCA83520.20759413755348619No Hit
CCCCATGTACTCTGCGTTGATACCA82390.20478545250277452No Hit
TATCAACGCAGAGTACTTTTTTTTT75140.18676512806236772No Hit
GGTATCAACGCAGAGTACTTTTTTT70260.1746355855424801No Hit
GCTTACCATGTACTCTGCGTTGATA61650.1532348967932522No Hit
GGAAGCAGTGGTATCAACGCAGAGT57500.14291981452736416No Hit
AAAAAGTACTCTGCGTTGATACCAC57040.14177645601114527No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55910.13896777096043358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA55080.13690475450725598No Hit
GAGTACATGGTAAGCAGTGGTATCA53650.13335040085901023No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52250.1298706140705179No Hit
TCCATGTACTCTGCGTTGATACCAC45450.11296879252641219No Hit
CTGCGTTGATACCACTGCTTCCCAT44790.11132832161183724No Hit
TGGGAAGCAGTGGTATCAACGCAGA44070.10953871697775547No Hit
CTCTGCGTTGATACCACTGCTTCCC43720.10866877028063238No Hit
ACTCTGCGTTGATACCACTGCTTAC42240.10499013853279761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGC550.003079847412.0856896
CTAGGAC2700.011.978943
ACACCGT802.8817198E-511.8698736
GACCGTG855.3557324E-511.1706737
TCGCGTT700.001499233910.85110710
ATCTACG1252.2019776E-710.6543432
CCGTACC909.572998E-510.550089
AAAAAGT11000.010.2092241
AAAAGTA11250.010.1469942
ACTCGCG750.002660560410.12807758
AGTACTC15050.010.1131565
CAGACCG856.629341E-410.0536069
GTACTAG2054.0017767E-119.7491971
TACGCAG1304.1714593E-69.5126895
TCTACAC3200.09.5126893
TCTACGC1201.6752949E-59.5126883
CTACGCA1201.6752949E-59.5126884
AGCCGTG900.00111962329.4950727
TTCCGTA1106.8808906E-59.4950727
GCCGAGC800.0045374599.4950728
Produced by FastQC (version 0.11.5)