FastQCFastQC Report
Wed 25 May 2016
SRR1294732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641745
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1241263.40842096302734No Hit
GTACATGGGAAGCAGTGGTATCAAC1235833.393510528606478No Hit
GAGTACATGGGAAGCAGTGGTATCA552931.5183105901154528No Hit
CATGTACTCTGCGTTGATACCACTG441271.2116993364444792No Hit
GCGTTGATACCACTGCTTCCCATGT402611.1055414368661178No Hit
GTATCAACGCAGAGTACATGGGAAG314380.8632674720498004No Hit
ACGCAGAGTACATGGGAAGCAGTGG297040.8156529356119112No Hit
ACTCTGCGTTGATACCACTGCTTCC262860.7217968309148499No Hit
TATCAACGCAGAGTACATGGGAAGC261480.7180074387415923No Hit
GCTTCCCATGTACTCTGCGTTGATA246190.676022071836441No Hit
GGTATCAACGCAGAGTACATGGGAA212310.5829897480466095No Hit
CAGTGGTATCAACGCAGAGTACATG151240.41529541469817355No Hit
GTGGTATCAACGCAGAGTACATGGG150340.4128240719764838No Hit
ATACCACTGCTTCCCATGTACTCTG143610.39434392029095944No Hit
GTACTCTGCGTTGATACCACTGCTT131640.36147506209248587No Hit
GCAGTGGTATCAACGCAGAGTACAT125090.34348917895129943No Hit
CTGCTTCCCATGTACTCTGCGTTGA114430.3142174973810632No Hit
ACATGGGAAGCAGTGGTATCAACGC110920.30457926076647324No Hit
GGGAAGCAGTGGTATCAACGCAGAG110280.30282186149771606No Hit
TACCACTGCTTCCCATGTACTCTGC109180.29980133150453975No Hit
GTACATGGTAAGCAGTGGTATCAAC108080.2967808015113634No Hit
CATGGGAAGCAGTGGTATCAACGCA108040.29667096405706606No Hit
GTTGATACCACTGCTTCCCATGTAC98370.2701177594806885No Hit
CTTCCCATGTACTCTGCGTTGATAC98160.2695411128456276No Hit
GATACCACTGCTTCCCATGTACTCT97740.26838781957550567No Hit
GCAGAGTACATGGGAAGCAGTGGTA94970.26078157586541617No Hit
CCATGTACTCTGCGTTGATACCACT93120.255701593604165No Hit
CCACTGCTTCCCATGTACTCTGCGT90580.2487269152562851No Hit
AAGCAGTGGTATCAACGCAGAGTAC87770.24101083409189825No Hit
ACCATGTACTCTGCGTTGATACCAC80430.22085566122833972No Hit
TTCCCATGTACTCTGCGTTGATACC76540.21017396879792516No Hit
ATGGGAAGCAGTGGTATCAACGCAG73300.2012771349998421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA73050.20059065091048386No Hit
TGATACCACTGCTTCCCATGTACTC70780.19435737537911085No Hit
GTACATGGGTAAGCAGTGGTATCAA52100.14306328422226158No Hit
ATCAACGCAGAGTACATGGGAAGCA51210.14061940086414615No Hit
CCCCATGTACTCTGCGTTGATACCA48430.1329856977904823No Hit
GGTAAGCAGTGGTATCAACGCAGAG44920.12334746117589233No Hit
GTATCAACGCAGAGTACTTTTTTTT38040.104455419036753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA3350.012.1932452
GTCCTAC1201.0017175E-811.8760311
TATACCG600.005887658311.0809465
CGCTATT600.005896691711.07866512
CTGATCG600.005897294311.0785129
GGTATCG1408.287316E-910.86360617
AAGCGGT1855.456968E-1210.77924110
GAACAAA16500.010.76761
TACACCG803.7808152E-410.6851985
AGCGGTG2100.010.40037811
CACCGTT750.002658611210.1289257
TCGTACC856.6013756E-410.0581762
TATCGAC1356.261962E-79.87318719
GTACAAA8450.09.5570421
GGGTAAG14850.09.3360047
TCTGGAC3900.09.2554333
CTTACAC3800.09.2490243
TAAGGTG2201.6916601E-109.0662295
TAGAACT3050.09.0309654
GTCGTAC950.00182324259.0007811
Produced by FastQC (version 0.11.5)