FastQCFastQC Report
Wed 25 May 2016
SRR1294732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641745
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1324923.6381459986901885No Hit
GTACATGGGAAGCAGTGGTATCAAC1227123.369593422933237No Hit
GAGTACATGGGAAGCAGTGGTATCA601301.6511315317244892No Hit
CATGTACTCTGCGTTGATACCACTG496901.3644557760084794No Hit
GCGTTGATACCACTGCTTCCCATGT425861.169384457176436No Hit
ACGCAGAGTACATGGGAAGCAGTGG368181.010998848079698No Hit
GTATCAACGCAGAGTACATGGGAAG340120.9339478738901268No Hit
TATCAACGCAGAGTACATGGGAAGC313510.8608785074188335No Hit
ACTCTGCGTTGATACCACTGCTTCC296820.815048829613276No Hit
GCTTCCCATGTACTCTGCGTTGATA269590.7402769826003743No Hit
GGTATCAACGCAGAGTACATGGGAA236720.6500180545315501No Hit
CAGTGGTATCAACGCAGAGTACATG186750.512803614750621No Hit
GTGGTATCAACGCAGAGTACATGGG160760.44143672882093615No Hit
ATACCACTGCTTCCCATGTACTCTG157280.4318808702970692No Hit
ACATGGGAAGCAGTGGTATCAACGC147090.4038997788148264No Hit
CATGGGAAGCAGTGGTATCAACGCA143870.395057863743892No Hit
GCAGTGGTATCAACGCAGAGTACAT142380.3909664185713168No Hit
GGGAAGCAGTGGTATCAACGCAGAG133710.3671591503523723No Hit
GTACTCTGCGTTGATACCACTGCTT125800.3454387937650769No Hit
CTGCTTCCCATGTACTCTGCGTTGA123220.33835427796289963No Hit
TACCACTGCTTCCCATGTACTCTGC119570.328331610258269No Hit
GTATCAACGCAGAGTACTTTTTTTT119030.32684880462525523No Hit
CTTCCCATGTACTCTGCGTTGATAC113360.31127934547860986No Hit
GATACCACTGCTTCCCATGTACTCT111560.30633666003523036No Hit
GCAGAGTACATGGGAAGCAGTGGTA109710.30125667777397924No Hit
CCATGTACTCTGCGTTGATACCACT109620.30100954350181025No Hit
AAGCAGTGGTATCAACGCAGAGTAC107060.29397994642678166No Hit
GTTGATACCACTGCTTCCCATGTAC104280.2863462433531178No Hit
GTACATGGTAAGCAGTGGTATCAAC103560.284369169175766No Hit
CCACTGCTTCCCATGTACTCTGCGT101160.2777789219179267No Hit
ATGGGAAGCAGTGGTATCAACGCAG92990.25534462187769874No Hit
TATCAACGCAGAGTACTTTTTTTTT92790.2547954346062121No Hit
ACCATGTACTCTGCGTTGATACCAC92100.25290073851958333No Hit
GGTATCAACGCAGAGTACTTTTTTT83140.22829714875698326No Hit
TTCCCATGTACTCTGCGTTGATACC79850.21926301814102853No Hit
TGATACCACTGCTTCCCATGTACTC72870.2000963823661459No Hit
GTACTTTTTTTTTTTTTTTTTTTTT66090.18147893386274988No Hit
ATCAACGCAGAGTACATGGGAAGCA63120.1733235028811737No Hit
GTACATGGGTAAGCAGTGGTATCAA56630.15550237592143326No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55790.15319578938118952No Hit
CCCCATGTACTCTGCGTTGATACCA54420.14943385657150624No Hit
GGTAAGCAGTGGTATCAACGCAGAG53840.14784121348419507No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43150.11848715382323582No Hit
GGAAGCAGTGGTATCAACGCAGAGT40050.1099747511151934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA38810.10656979003197643No Hit
GAGTACATGGTAAGCAGTGGTATCA38640.10610298085121281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG400.00530412814.24057814
TAGTCGT400.00530412814.24057815
GACTGCG500.001507405413.2915727
GACCGTC604.1163253E-412.6586397
CACGACT550.003032464312.11068254
CTGATCG550.003084732512.083089
TTACGCT600.0058071611.10145954
GACCGCT600.005906028711.0763097
GGTCTAG1355.5213604E-810.5779141
CTAGACA2151.8189894E-1210.1794124
ACGACTG750.00261336310.1499055
AGTACTC12250.010.0981215
GGCTTAG1553.93793E-89.8272231
GTATTAC2350.09.7226771
AAGTACT13400.09.5865484
CGTACCC1002.712238E-49.5155373
TAGACAG3200.09.5155365
AAGACCG800.00445741469.5155365
CCTACAC3100.09.5155363
TAACGTG800.00445741469.5155365
Produced by FastQC (version 0.11.5)