FastQCFastQC Report
Wed 25 May 2016
SRR1294731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873138
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64640.22498049171324175No Hit
CCCATGTACTCTGCGTTGATACCAC56750.1975192281052981No Hit
GTATCAACGCAGAGTACTTTTTTTT39210.13647099443187205No Hit
GAGTACATGGGAAGCAGTGGTATCA30730.10695622695463984No Hit
GGTATCAACGCAGAGTACTTTTTTT30160.10497233338600512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGG1053.4631285E-610.85866451
CTAGAAC3400.010.3378853
ACACCGT856.617663E-410.0553316
CTAGCCT2850.09.999144
GTCCTAA2500.09.5013321
GTCTACG800.00451219559.5013321
TGTACCG1106.8543864E-59.498195
GCACCGT1002.766295E-49.4967026
TGGACTG5550.09.4126215
CCTATAC3250.09.3535293
GTCCTGG5900.09.3402921
CTGTCGC1856.7429937E-99.2400349
GTCCTAG2750.08.9830781
TATACTG7250.08.9086475
TAGGACA3550.08.8302254
TCGCCCA2054.1709427E-98.80182112
CTAGGAC2607.2759576E-128.7689333
ACAGCGT1107.171709E-48.6333668
GTACCGT1000.0029151068.5470326
TTATACT4450.08.5385914
Produced by FastQC (version 0.11.5)