##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294731_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2873138
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00004803110745	33.0	33.0	33.0	27.0	33.0
2	31.024444005126103	33.0	33.0	33.0	27.0	33.0
3	30.994720058695407	33.0	33.0	33.0	27.0	33.0
4	34.52564234645186	37.0	37.0	37.0	27.0	37.0
5	34.690792784753114	37.0	37.0	37.0	27.0	37.0
6	34.868216215162654	37.0	37.0	37.0	33.0	37.0
7	34.853067621534365	37.0	37.0	37.0	33.0	37.0
8	34.75364427326498	37.0	37.0	37.0	27.0	37.0
9	34.46846444549479	37.0	37.0	37.0	27.0	37.0
10	34.557663780855634	37.0	37.0	37.0	27.0	37.0
11	34.76751934644281	37.0	37.0	37.0	27.0	37.0
12	34.6923235152645	37.0	37.0	37.0	27.0	37.0
13	34.616156272340554	37.0	37.0	37.0	27.0	37.0
14	35.72904503716842	37.0	37.0	40.0	27.0	40.0
15	36.042113187741066	40.0	37.0	40.0	27.0	40.0
16	36.06300985194585	40.0	37.0	40.0	27.0	40.0
17	36.00695058852029	40.0	37.0	40.0	27.0	40.0
18	36.009139484424345	37.0	37.0	40.0	27.0	40.0
19	36.10063108698573	40.0	37.0	40.0	27.0	40.0
20	35.978888239966196	40.0	37.0	40.0	27.0	40.0
21	35.93092604671269	40.0	37.0	40.0	27.0	40.0
22	35.78996379568263	37.0	37.0	40.0	27.0	40.0
23	35.39800455112146	37.0	33.0	40.0	27.0	40.0
24	35.71452398040052	37.0	37.0	40.0	27.0	40.0
25	34.49070911317173	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5765948597602701
1101	2	0.6698695907154288
1101	3	0.6058174110790944
1101	4	0.772220085908522
1101	5	0.7748823105572953
1101	6	0.5561322969815876
1101	7	0.5598269807927636
1101	8	0.5962428760984082
1101	9	0.8404604591418234
1101	10	0.8518266370577905
1101	11	0.554094259855809
1101	12	0.7759193314837205
1101	13	0.6491764900260222
1101	14	1.3788796184715437
1101	15	1.1636443777962882
1101	16	1.1520471230053673
1101	17	1.1654390024835521
1101	18	1.1773154118593183
1101	19	1.0854072306911888
1101	20	1.1687142524952066
1101	21	1.203046646052421
1101	22	1.2525973752427575
1101	23	1.5039619062432834
1101	24	1.377811811094496
1101	25	1.162123553611309
1102	1	0.30075598753810695
1102	2	0.5549959515859619
1102	3	0.38451892592543757
1102	4	0.35438998756895046
1102	5	0.35205970341026926
1102	6	0.2908977025130568
1102	7	0.376776422310229
1102	8	0.395315045964729
1102	9	0.6470414894634899
1102	10	0.6743185009866366
1102	11	0.5496176539314064
1102	12	0.47606358994399756
1102	13	0.5588391566785518
1102	14	1.2276803209475275
1102	15	0.9178144455350434
1102	16	0.6310289391955308
1102	17	0.9391572841735965
1102	18	0.863921636616297
1102	19	0.930422584102331
1102	20	0.9410902149544427
1102	21	0.9919067656994898
1102	22	1.0052113420765636
1102	23	1.426765078692661
1102	24	1.07211638460889
1102	25	0.9949564434801772
1103	1	0.39938179426447107
1103	2	0.5086366894445788
1103	3	0.3632760995150157
1103	4	0.2535125738423716
1103	5	0.46971544466936166
1103	6	0.2722716957585547
1103	7	0.3144281371965789
1103	8	0.5311036601053019
1103	9	0.656630902684185
1103	10	0.7821570550542418
1103	11	0.5503458208606915
1103	12	0.5791782538128558
1103	13	0.5406148193201332
1103	14	1.2327332896876442
1103	15	0.9723050125954416
1103	16	0.7141624057088904
1103	17	0.756314471442515
1103	18	0.8470215177608722
1103	19	0.7672015633394125
1103	20	0.9521843559368506
1103	21	1.0119620057267937
1103	22	0.8980320742827743
1103	23	1.378852049634375
1103	24	1.0284781503281977
1103	25	0.8966871385069624
1104	1	0.35726697862567036
1104	2	0.580214210053466
1104	3	0.39339872142730314
1104	4	0.3454449120846732
1104	5	0.38208505952975713
1104	6	0.3124349430251243
1104	7	0.22908948105533966
1104	8	0.4393519772558321
1104	9	0.5948673224704208
1104	10	0.6785419440590559
1104	11	0.5307208169183397
1104	12	0.3696069702102065
1104	13	0.433393792588042
1104	14	0.9735917325756063
1104	15	0.8408519231165243
1104	16	0.6518300620112285
1104	17	0.5814539026705106
1104	18	0.5885217108293332
1104	19	0.6726486830392986
1104	20	0.8524528830503968
1104	21	0.9295029677788236
1104	22	0.8623400879450287
1104	23	1.2597376542284664
1104	24	1.0028345674890389
1104	25	0.8937368244404098
1105	1	0.40816879406943585
1105	2	0.4545694946588199
1105	3	0.5315314114829022
1105	4	0.560285030315427
1105	5	0.5455347001293376
1105	6	0.5148828006451112
1105	7	0.41140881202156265
1105	8	0.48287509990069566
1105	9	-1.1693705939759909
1105	10	0.11075557526952906
1105	11	0.3359540914988983
1105	12	0.5982572130115713
1105	13	0.492090938460926
1105	14	-1.1991707470068107
1105	15	0.07480064503119621
1105	16	0.19947037604679707
1105	17	0.43350271449902067
1105	18	0.48178110461626744
1105	19	0.5506865683813018
1105	20	0.6673036668645409
1105	21	0.8186984980920329
1105	22	0.8939231749642929
1105	23	1.104513685475652
1105	24	0.9502562158188326
1105	25	0.691899900785593
1106	1	0.2545891363779553
1106	2	0.22086837919654556
1106	3	0.30482340490936366
1106	4	-0.7831588600480615
1106	5	-0.10295414978470063
1106	6	0.024230981900927873
1106	7	0.17631847927231803
1106	8	0.3861837513383506
1106	9	0.5376537920500652
1106	10	0.5227248980554577
1106	11	0.43590504083474
1106	12	0.37162371647848147
1106	13	0.42330792333421385
1106	14	0.885636089983258
1106	15	0.5627942807208655
1106	16	0.3833503743174802
1106	17	0.19093575820748754
1106	18	0.35726724793708087
1106	19	0.31273170886955626
1106	20	0.48054387688038247
1106	21	0.7640094627568672
1106	22	0.6965305460141522
1106	23	1.0321452215833986
1106	24	0.6037692628151348
1106	25	0.6548760431616856
1107	1	0.4575682602589026
1107	2	0.44605427260207264
1107	3	0.34759653991564576
1107	4	0.5438311171284695
1107	5	0.31756404403471095
1107	6	0.287965331077622
1107	7	0.37748297418968946
1107	8	0.33671053719727695
1107	9	0.5906891416751634
1107	10	0.4930141694987995
1107	11	0.26069669686706476
1107	12	0.29343322261807003
1107	13	0.2781428024297625
1107	14	0.8669094174010965
1107	15	0.6440297982743317
1107	16	0.576803124060504
1107	17	0.6429685503941442
1107	18	0.6792586815728185
1107	19	0.5761450562713009
1107	20	0.7888044916865056
1107	21	0.8375566485200636
1107	22	0.7537099423584266
1107	23	0.9515712696762009
1107	24	0.9166165779021256
1107	25	0.7691820793216095
1108	1	0.48906108263228276
1108	2	0.43149669951770164
1108	3	0.5370614359233024
1108	4	0.6488968105380835
1108	5	0.5604421865436464
1108	6	0.4440864865897396
1108	7	0.3891513345427242
1108	8	0.5290276875375284
1108	9	0.2963344650107018
1108	10	0.5478501125505773
1108	11	0.4310912320979057
1108	12	0.38184907090990095
1108	13	0.454277536261543
1108	14	-0.7682002541967208
1108	15	0.05769996618711559
1108	16	0.523930761706751
1108	17	0.6517063576540707
1108	18	0.7220450911450982
1108	19	0.6397262443886831
1108	20	0.7733498846433591
1108	21	0.8309962732856491
1108	22	0.9462734051470534
1108	23	1.1022217749713121
1108	24	1.0268581191999644
1108	25	0.9591705045968837
1109	1	0.21776245639513192
1109	2	0.2349401344980251
1109	3	0.32956253455144946
1109	4	0.355154644234446
1109	5	0.34317194312585997
1109	6	0.23401359500045515
1109	7	0.1476256266440643
1109	8	0.3853956706473127
1109	9	0.5317464027996834
1109	10	0.5613792405264277
1109	11	0.4511568154216832
1109	12	0.342503423388024
1109	13	0.27157567528099236
1109	14	0.6662518169561693
1109	15	0.47726233005275986
1109	16	0.44295107199755535
1109	17	0.3963222677003131
1109	18	0.5260854155747694
1109	19	0.4179764641143109
1109	20	0.7058803596328502
1109	21	0.58913577040056
1109	22	0.6292436179943195
1109	23	1.0981775814599075
1109	24	0.7880600661601136
1109	25	0.5976186206884435
1110	1	0.45329487869043206
1110	2	0.29053787883332305
1110	3	0.41291325518982447
1110	4	0.6251895960279441
1110	5	0.6367206633949891
1110	6	0.34053673805519225
1110	7	0.4139954354221729
1110	8	0.3799333297529728
1110	9	0.5251315342231209
1110	10	0.45649051990923795
1110	11	0.29859286103588545
1110	12	0.37403660158182817
1110	13	0.2718471253198089
1110	14	0.7774442317151866
1110	15	0.4918482769991286
1110	16	0.3861032860462217
1110	17	0.45990168357766237
1110	18	0.5645623235942523
1110	19	0.5554497352707699
1110	20	0.5896093227277532
1110	21	0.6862941708618138
1110	22	0.7552969765595776
1110	23	0.874070604796664
1110	24	0.712849252574216
1110	25	0.8113182312380331
1111	1	0.09274430377048049
1111	2	0.12361139410538158
1111	3	0.15298775469752002
1111	4	0.3696466451333933
1111	5	0.3036212471850348
1111	6	0.15886256889651662
1111	7	0.11199202444656464
1111	8	0.28109008273746383
1111	9	0.12069127705846228
1111	10	-0.019264284483689664
1111	11	0.033604963312654945
1111	12	0.06924309813778962
1111	13	0.09473849900435027
1111	14	-1.7287620350363824
1111	15	-0.6512309069046012
1111	16	-0.14525789765760067
1111	17	0.11938512599299145
1111	18	0.1991240268140544
1111	19	0.1553112518975297
1111	20	0.23950854012201717
1111	21	0.24645742856302633
1111	22	0.04670965345362532
1111	23	-2.159506345226262
1111	24	-0.5097441008985371
1111	25	-0.0892671299585146
1112	1	-0.28747245592240844
1112	2	-0.34524206039526106
1112	3	-0.15004065592481197
1112	4	0.09523228043837406
1112	5	-0.9724843555342417
1112	6	-1.0693850707237544
1112	7	-1.1799187561775923
1112	8	-0.8994047147887798
1112	9	-0.7570099613690289
1112	10	-1.0072290582904273
1112	11	-0.9655472050767457
1112	12	-0.8459020797047785
1112	13	-0.9544203365312569
1112	14	-0.7994727537258441
1112	15	-1.2063566915473345
1112	16	-1.0325740504798802
1112	17	-1.0324107340727124
1112	18	-1.2211917904608924
1112	19	-1.0422270561970493
1112	20	-1.1454583524057966
1112	21	-2.237742112237001
1112	22	-2.0918232591970067
1112	23	-1.6232929943799803
1112	24	-1.8572491274481422
1112	25	-2.0702350352242647
1113	1	0.06730246218513969
1113	2	-0.1037944229865353
1113	3	-0.044620808381893085
1113	4	0.1717879921177925
1113	5	0.028505003458924705
1113	6	-0.02857310508624522
1113	7	-0.008592685824247326
1113	8	-0.06891943991779925
1113	9	0.21081695687023938
1113	10	-0.10592333302614776
1113	11	-0.16600239548752427
1113	12	0.007551682186281994
1113	13	-0.17609639739107763
1113	14	-0.17305161609726127
1113	15	-0.22483718454675028
1113	16	0.013423204969690516
1113	17	-0.09785838273033676
1113	18	0.05282622356558164
1113	19	-0.169850873713024
1113	20	-0.8665959628434692
1113	21	-0.7319742382094461
1113	22	-0.1619124856617873
1113	23	-0.43232176153943414
1113	24	-0.10149247964243813
1113	25	-1.116793374973554
1114	1	-0.8834072319924893
1114	2	-0.7455275124630916
1114	3	-0.5790772965200937
1114	4	-1.6769923210330688
1114	5	-0.8107095079646172
1114	6	-0.6335293430591094
1114	7	-0.5863208751913902
1114	8	-0.5203279851505727
1114	9	-0.43121433005416065
1114	10	-0.5670451112500672
1114	11	-0.6736537214286429
1114	12	-0.7141470965196248
1114	13	-0.656810368603864
1114	14	-0.793907456668066
1114	15	-1.592187074461961
1114	16	-1.0363221785049106
1114	17	-1.000356988316831
1114	18	-0.8805985328995547
1114	19	-0.8385099236072264
1114	20	-2.095678532781143
1114	21	-6.679736835615753
1114	22	-7.977022888658176
1114	23	-8.009060637565721
1114	24	-6.261726177880885
1114	25	-7.287609794008496
1115	1	-1.280986825145245
1115	2	-1.098559442409087
1115	3	-1.005993433453149
1115	4	-0.8538406698122358
1115	5	-0.9054063051099206
1115	6	-1.1832553803164672
1115	7	-1.2770085576647716
1115	8	-1.0393007801621508
1115	9	-3.181925883817808
1115	10	-2.0558739685932252
1115	11	-1.4985711677823303
1115	12	-1.364290610203959
1115	13	-1.7714017810526315
1115	14	-1.2271664941847433
1115	15	-2.343187824815203
1115	16	-1.6468919046604924
1115	17	-3.2443367593456998
1115	18	-3.0318960721156216
1115	19	-2.356446373322221
1115	20	-2.102813001800236
1115	21	-1.557368125247173
1115	22	-1.194133062402912
1115	23	-0.5151949478372799
1115	24	-1.6696535957487555
1115	25	-1.4590995754989322
1116	1	-1.6634604463905163
1116	2	-0.9706944240328781
1116	3	-0.7203712469175407
1116	4	-0.4688360533056226
1116	5	-0.39281797175862465
1116	6	-0.45240274567434113
1116	7	-0.946309147930954
1116	8	-1.7095762257358729
1116	9	-1.1011768960362787
1116	10	-2.6970695539914082
1116	11	-1.1592374536321088
1116	12	-0.9559923946544089
1116	13	-0.8897267974888265
1116	14	-1.3630442950749924
1116	15	-1.3857936901138004
1116	16	-0.8914765807326148
1116	17	-0.701074164545652
1116	18	-1.519983688740794
1116	19	-1.0531744018044549
1116	20	-1.319674926937381
1116	21	-0.8093683120630004
1116	22	-0.35428542508429217
1116	23	-0.17925032436099286
1116	24	-0.18221696044559366
1116	25	-0.28772101836382546
1201	1	0.37937805333049823
1201	2	0.4927660456669578
1201	3	0.4409283167021165
1201	4	0.44692374399772916
1201	5	0.4931895611667372
1201	6	0.37393133230191467
1201	7	0.4641997985961481
1201	8	0.5719487009503439
1201	9	0.810095637544272
1201	10	0.7749691874012541
1201	11	0.6141321637167962
1201	12	0.6185244792055897
1201	13	0.6193942004250985
1201	14	1.2015550957187529
1201	15	0.9534655518955191
1201	16	0.9521790855860317
1201	17	0.9878011215338134
1201	18	0.8646923028735216
1201	19	0.7774251549583511
1201	20	0.9926343033635447
1201	21	1.0038091138529737
1201	22	1.0436662431727584
1201	23	0.7705283250315844
1201	24	0.9273244677791652
1201	25	0.8832324738895068
1202	1	-0.11939852451584798
1202	2	0.16374330107293034
1202	3	0.05992659616551066
1202	4	0.2318648391511644
1202	5	0.16823603884213156
1202	6	-0.018678116930388455
1202	7	0.06104900555657622
1202	8	0.20381851542884988
1202	9	0.30281889669826967
1202	10	0.5382208111179949
1202	11	0.3473227308810749
1202	12	0.2607253527512583
1202	13	0.34193654238099214
1202	14	0.5838897973658916
1202	15	0.4794248414544029
1202	16	0.5799070801492121
1202	17	0.27122001243501614
1202	18	0.29141574476379617
1202	19	0.46948846562631275
1202	20	0.6210525552398352
1202	21	0.6347968807355855
1202	22	0.5582729171323138
1202	23	0.8676726428027948
1202	24	0.5008334487704502
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2211	5	0.30484247720274027
2211	6	0.33353169217705414
2211	7	0.2813448433063712
2211	8	0.3701946432885066
2211	9	0.5306577940021455
2211	10	0.5269215332481139
2211	11	0.20138578313984112
2211	12	0.24353529857335587
2211	13	0.09376465344979579
2211	14	0.5022624381793293
2211	15	0.28117005777173176
2211	16	0.20171050420799475
2211	17	0.3395532981971954
2211	18	0.3106931889253417
2211	19	0.3730034256254271
2211	20	0.32201710473213296
2211	21	0.3511102872381855
2211	22	0.43142972439512306
2211	23	0.5671738229705596
2211	24	0.5428340205918474
2211	25	0.5294500277540024
2212	1	-0.3668056402589528
2212	2	-0.3864871023329819
2212	3	-0.3628418130358604
2212	4	-0.6491055006400259
2212	5	-0.3057969743668423
2212	6	-0.7456639235883955
2212	7	-0.6629000288409017
2212	8	-0.5141455674794599
2212	9	-0.42244426767256726
2212	10	-0.6903676680964779
2212	11	-0.6236002977701531
2212	12	-0.8618763292858915
2212	13	-0.82822729320511
2212	14	-0.6533783380584026
2212	15	-0.7849958277921232
2212	16	-0.6348498119580057
2212	17	-0.67812692156933
2212	18	-0.5396238083335803
2212	19	-0.7571977243196102
2212	20	-0.5893226731640766
2212	21	-0.6640717124193785
2212	22	-0.6698086130662233
2212	23	-0.3352620013792844
2212	24	-0.35450766516387944
2212	25	-1.2680139754315718
2213	1	-0.28101870528714556
2213	2	-1.3273177647177903
2213	3	-0.40522311372481923
2213	4	-0.04016905119048886
2213	5	-0.10719107810552231
2213	6	-0.11786813256048134
2213	7	-0.12426574193199258
2213	8	0.05649272640066272
2213	9	0.03982395678148265
2213	10	-0.5596738848902945
2213	11	-0.27760817215379063
2213	12	-0.016099726332875264
2213	13	-0.42191729371433695
2213	14	-0.11433340287833005
2213	15	-0.25088156572748943
2213	16	-0.17876436911249272
2213	17	-0.08615582807104971
2213	18	0.04173638214971476
2213	19	0.05933822927144661
2213	20	0.11092523274211175
2213	21	-0.548459474727494
2213	22	-0.9684176785725569
2213	23	-0.15385395016204484
2213	24	-0.07165469493485688
2213	25	-0.579346836194226
2214	1	-0.3736589769187155
2214	2	-0.6484965333233177
2214	3	-0.7153725419819246
2214	4	-0.9076194560203064
2214	5	-0.6221648614524256
2214	6	-0.40859855291355274
2214	7	-0.7765540571911913
2214	8	-0.33747995089316873
2214	9	-0.28774037976759104
2214	10	-2.139970693079704
2214	11	-1.1944135133723321
2214	12	-0.7494893039568709
2214	13	-0.44702345005187993
2214	14	-1.134511306659121
2214	15	-0.8069663282858031
2214	16	-1.0281902350957637
2214	17	-0.9153010354671096
2214	18	-0.8614633424267737
2214	19	-1.6172992508827662
2214	20	-2.796298730626731
2214	21	-2.2770508938124223
2214	22	-2.5095867853484677
2214	23	-2.7920366289942393
2214	24	-1.851319484887398
2214	25	-2.2679881345274424
2215	1	-0.2594457459686339
2215	2	-0.2868450745584141
2215	3	-0.7443826911598812
2215	4	-0.35322593626363386
2215	5	-0.27019266932367003
2215	6	-0.19646396184972303
2215	7	-0.18192676032962396
2215	8	-0.175200684559222
2215	9	-0.28757915938825107
2215	10	-0.2644317682549371
2215	11	-0.5643192440239986
2215	12	-0.44763096333311125
2215	13	-0.400809711416251
2215	14	-0.3075187542247235
2215	15	-0.7130635410015174
2215	16	-0.5376652187395266
2215	17	-0.2626096221521479
2215	18	-0.3134908360164914
2215	19	-2.1426611657034016
2215	20	-2.560900809417717
2215	21	-3.625690795176375
2215	22	-2.7012614979244134
2215	23	-3.6224069523566875
2215	24	-2.3782351290058727
2215	25	-1.8409520661893453
2216	1	-1.8325726972055918
2216	2	-1.46784507769463
2216	3	-1.061944855117705
2216	4	-1.1907381524152711
2216	5	-2.02830044261988
2216	6	-1.783474323655284
2216	7	-1.3564623053201643
2216	8	-5.683874483938588
2216	9	-4.2554745941963965
2216	10	-2.3048474177633835
2216	11	-1.4136536552495116
2216	12	-1.486127612531078
2216	13	-2.246630599413173
2216	14	-3.9881375868925737
2216	15	-2.4010969836439244
2216	16	-3.3925648394266616
2216	17	-3.0022016780770926
2216	18	-2.939504465996947
2216	19	-1.9320532639430965
2216	20	-1.1305677942893482
2216	21	-1.029931364886302
2216	22	-0.8420919236266897
2216	23	-1.3142894929881308
2216	24	-0.7950889431350916
2216	25	-0.6609254952302379
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	42.0
8	185.0
9	553.0
10	1255.0
11	2628.0
12	4555.0
13	7121.0
14	9621.0
15	12153.0
16	13972.0
17	15159.0
18	15888.0
19	16508.0
20	16992.0
21	18600.0
22	19847.0
23	21480.0
24	22778.0
25	24592.0
26	26718.0
27	30449.0
28	35093.0
29	41414.0
30	49675.0
31	61221.0
32	81189.0
33	114167.0
34	163139.0
35	254004.0
36	432771.0
37	1359366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8996577619792	20.475721293610977	12.886392992302257	23.738227952107565
2	15.918685896811096	20.46400753016662	39.422469081481196	24.194837491541094
3	18.909687144264545	24.67090445141459	29.152884002826735	27.26652440149413
4	12.497963469162396	16.308156010098408	37.46562266321141	33.72825785752778
5	13.447700023637346	37.68847649824739	35.013035351930135	13.850788126185131
6	32.557849743818224	37.510616228558696	16.585925317442637	13.34560871018044
7	27.25759335527683	32.68768596833357	22.036436132352897	18.018284544036707
8	24.725264780918373	37.10173225715509	20.227626316943542	17.945376644982996
9	24.24496714373225	16.98261819903102	21.456012975441332	37.316401681795405
10	14.63599441584419	30.549689902541793	33.37316215178643	21.441153529827588
11	33.35291714030939	25.151490260420545	22.29553774604768	19.200054853222383
12	20.88450309698599	27.274708017358158	30.921097512248025	20.919691373407826
13	27.062682492263495	23.391483734977292	26.19569957818596	23.350134194573254
14	20.467511204683117	22.98706392925622	27.557203313878354	28.98822155218231
15	23.111870648203418	30.78471126085284	23.365360351815724	22.738057739128013
16	22.028504581491234	30.72327904320169	25.462586866647037	21.785629508660037
17	20.599544113414055	30.00400614949168	27.852171343466235	21.54427839362803
18	20.922969507458156	29.543113073840903	28.67290198447946	20.861015434221482
19	21.422657024688398	28.71811274877477	28.82193676515272	21.037293461384113
20	22.308160304727114	28.32877616312256	28.35891752821315	21.004146003937173
21	22.385811697630544	28.326898586910183	27.875398136872697	21.411891578586577
22	21.53461622055406	28.165037979480488	28.641766839984616	21.65857895998083
23	21.75885692829783	28.35909844684833	28.301104736640635	21.580939888213205
24	21.665845257685742	28.139000498414262	28.35566451986557	21.83948972403443
25	22.058270552114283	27.99715644505402	27.757519270386776	22.18705373244492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2157.0
1	2157.0
2	2501.5
3	2846.0
4	2846.0
5	2846.0
6	4216.5
7	5587.0
8	5587.0
9	5587.0
10	8666.0
11	11745.0
12	11745.0
13	11745.0
14	18932.5
15	26120.0
16	26120.0
17	26120.0
18	40460.0
19	54800.0
20	54800.0
21	54800.0
22	77140.0
23	99480.0
24	99480.0
25	99480.0
26	128061.0
27	156642.0
28	156642.0
29	156642.0
30	187857.0
31	219072.0
32	219072.0
33	219072.0
34	245677.5
35	272283.0
36	272283.0
37	272283.0
38	292011.0
39	311739.0
40	311739.0
41	311739.0
42	316745.0
43	321751.0
44	321751.0
45	321751.0
46	329176.5
47	336602.0
48	336602.0
49	336602.0
50	323014.0
51	309426.0
52	309426.0
53	309426.0
54	284640.0
55	259854.0
56	259854.0
57	259854.0
58	230533.5
59	201213.0
60	201213.0
61	201213.0
62	170263.0
63	139313.0
64	139313.0
65	139313.0
66	109505.5
67	79698.0
68	79698.0
69	79698.0
70	59324.0
71	38950.0
72	38950.0
73	38950.0
74	27527.5
75	16105.0
76	16105.0
77	16105.0
78	10769.5
79	5434.0
80	5434.0
81	5434.0
82	3573.0
83	1712.0
84	1712.0
85	1712.0
86	1085.5
87	459.0
88	459.0
89	459.0
90	289.5
91	120.0
92	120.0
93	120.0
94	70.5
95	21.0
96	21.0
97	21.0
98	15.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03021087048377071
2	0.014826994039269955
3	0.019769325385693273
4	0.021091921098116417
5	0.0196649099347125
6	0.0061953167581926104
7	3.480515032692478E-5
8	2.7844120261539824E-4
9	6.26492705884646E-4
10	3.13246352942323E-4
11	5.568824052307965E-4
12	7.657133071923451E-4
13	0.002471165673211659
14	3.13246352942323E-4
15	0.0014966214640577654
16	0.0019490884183077874
17	0.0019142832679808627
18	0.0014618163137308407
19	2.4363605228847342E-4
20	3.480515032692478E-4
21	0.07886847064081155
22	0.07357808779111898
23	0.07437860624863825
24	9.049339085000442E-4
25	0.21941166766093378
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2873138.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.4402326053478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.62851304147293	68.73600945833891
2	9.000196587747833	14.119550276735954
3	1.8071533619705455	4.252605901995176
4	0.597951261625865	1.8761374419437613
5	0.26789077322876653	1.0506707282445464
6	0.15545344295351188	0.7316282534745383
7	0.10161096580300444	0.5579271454989196
8	0.07834693472108446	0.4916441426750292
9	0.057242644435468004	0.4041113710016996
>10	0.2801242760505683	3.8883717964350377
>50	0.016996421944043664	0.9065495132110293
>100	0.00695981710605543	1.0192702717552153
>500	7.133581442960218E-4	0.3717925064137356
>1k	7.579430280581493E-4	1.1692001759932047
>5k	8.916976800684108E-5	0.4245310162832469
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6464	0.22498049171324175	No Hit
CCCATGTACTCTGCGTTGATACCAC	5675	0.1975192281052981	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3921	0.13647099443187205	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3073	0.10695622695463984	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3016	0.10497233338600512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.480515032692478E-5	0.0
2	0.0	0.0	0.0	3.480515032692478E-5	0.0
3	0.0	0.0	0.0	6.961030065384956E-5	0.0
4	0.0	0.0	0.0	6.961030065384956E-5	0.0
5	0.0	0.0	0.0	6.961030065384956E-5	0.0
6	0.0	0.0	0.0	6.961030065384956E-5	0.0
7	0.0	0.0	0.0	6.961030065384956E-5	0.0
8	0.0	0.0	0.0	6.961030065384956E-5	0.0
9	0.0	0.0	0.0	1.0441545098077433E-4	0.0
10	0.0	0.0	0.0	1.0441545098077433E-4	0.0
11	0.0	0.0	0.0	1.0441545098077433E-4	0.0
12	0.0	0.0	0.0	1.3922060130769912E-4	0.0
13	0.0	0.0	0.0	1.3922060130769912E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTGG	105	3.4631285E-6	10.8586645	1
CTAGAAC	340	0.0	10.337885	3
ACACCGT	85	6.617663E-4	10.055331	6
CTAGCCT	285	0.0	9.99914	4
GTCCTAA	250	0.0	9.501332	1
GTCTACG	80	0.0045121955	9.501332	1
TGTACCG	110	6.8543864E-5	9.49819	5
GCACCGT	100	2.766295E-4	9.496702	6
TGGACTG	555	0.0	9.412621	5
CCTATAC	325	0.0	9.353529	3
GTCCTGG	590	0.0	9.340292	1
CTGTCGC	185	6.7429937E-9	9.240034	9
GTCCTAG	275	0.0	8.983078	1
TATACTG	725	0.0	8.908647	5
TAGGACA	355	0.0	8.830225	4
TCGCCCA	205	4.1709427E-9	8.801821	12
CTAGGAC	260	7.2759576E-12	8.768933	3
ACAGCGT	110	7.171709E-4	8.633366	8
GTACCGT	100	0.002915106	8.547032	6
TTATACT	445	0.0	8.538591	4
>>END_MODULE