FastQCFastQC Report
Wed 25 May 2016
SRR1294731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873138
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108110.37627848018438376No Hit
GGTATCAACGCAGAGTACTTTTTTT91500.3184671254913617No Hit
TATCAACGCAGAGTACTTTTTTTTT73380.25540019309897405No Hit
GTACATGGGAAGCAGTGGTATCAAC68340.23785839733420394No Hit
CCCATGTACTCTGCGTTGATACCAC62970.21916803160864531No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47100.1639322580398157No Hit
GAGTACATGGGAAGCAGTGGTATCA35510.12359308881090987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAG456.695824E-414.7977675
CAGCGTA604.1122327E-412.6599619
ACCGTTC701.0969308E-412.207828
CCGTATC658.062798E-411.6861179
CACGACT951.3409548E-511.0148794
TATTCCG1201.4986999E-610.3055885
ACCGCTC750.002660402310.1279688
GGGCGCA750.002660402310.12796811
TGGACCG856.515097E-410.0724315
AGCTCGT856.628761E-410.05349811
GCTCGTC856.629822E-410.05332412
GGACTGT4950.09.9753826
TTAGACA2900.09.8408794
GCACCGT1451.6290142E-79.8232386
TAATACC3300.09.5128514
CTTCGTC800.00453719369.494979
ACAGCGT1106.879856E-59.494978
CTATCGG900.00111970679.49480512
TAGACAG4350.09.4035075
GTCTTAG2650.09.337271
Produced by FastQC (version 0.11.5)