FastQCFastQC Report
Wed 25 May 2016
SRR1294730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195087
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1360533.243150857181269No Hit
CCCATGTACTCTGCGTTGATACCAC1317463.1404831413508227No Hit
GAGTACATGGGAAGCAGTGGTATCA595721.4200420634899824No Hit
CATGTACTCTGCGTTGATACCACTG489191.1661021571185533No Hit
GCGTTGATACCACTGCTTCCCATGT416360.9924943153741508No Hit
GTATCAACGCAGAGTACATGGGAAG327030.7795547505927768No Hit
ACGCAGAGTACATGGGAAGCAGTGG312870.7458009810046848No Hit
GCTTCCCATGTACTCTGCGTTGATA300600.7165524815099187No Hit
TATCAACGCAGAGTACATGGGAAGC266970.6363872787382002No Hit
ACTCTGCGTTGATACCACTGCTTCC263510.6281395356043867No Hit
GGTATCAACGCAGAGTACATGGGAA206060.4911936272120221No Hit
CAGTGGTATCAACGCAGAGTACATG173980.4147232226649888No Hit
GCAGTGGTATCAACGCAGAGTACAT149390.35610703663595056No Hit
GTGGTATCAACGCAGAGTACATGGG147960.3526982873060797No Hit
GTACATGGTAAGCAGTGGTATCAAC144800.34516566640930213No Hit
GTACTCTGCGTTGATACCACTGCTT142490.3396592251841261No Hit
ATACCACTGCTTCCCATGTACTCTG142130.3388010784996831No Hit
CTGCTTCCCATGTACTCTGCGTTGA125140.2983013224755529No Hit
GGGAAGCAGTGGTATCAACGCAGAG124810.2975146880148135No Hit
CATGGGAAGCAGTGGTATCAACGCA115260.2747499634691724No Hit
TACCACTGCTTCCCATGTACTCTGC114600.2731766945476935No Hit
GCAGAGTACATGGGAAGCAGTGGTA108630.2589457620306802No Hit
ACCATGTACTCTGCGTTGATACCAC107200.2555370127008093No Hit
AAGCAGTGGTATCAACGCAGAGTAC106170.25308175968698626No Hit
ACATGGGAAGCAGTGGTATCAACGC103020.24557297619810983No Hit
GTTGATACCACTGCTTCCCATGTAC100580.2397566486702183No Hit
CCACTGCTTCCCATGTACTCTGCGT98850.23563277710331154No Hit
CTTCCCATGTACTCTGCGTTGATAC97480.23236705222084786No Hit
CCATGTACTCTGCGTTGATACCACT96610.2302931977334439No Hit
GATACCACTGCTTCCCATGTACTCT95170.22686061099567184No Hit
GTACATGGGTAAGCAGTGGTATCAA78160.18631318015573933No Hit
ATGGGAAGCAGTGGTATCAACGCAG78070.18609864348462857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA71760.17105723909897458No Hit
TTCCCATGTACTCTGCGTTGATACC67530.16097401555676916No Hit
GGTAAGCAGTGGTATCAACGCAGAG67390.16064029184615242No Hit
TGATACCACTGCTTCCCATGTACTC66200.15780364030591024No Hit
CCCCATGTACTCTGCGTTGATACCA56320.13425228129952965No Hit
ATCAACGCAGAGTACATGGGAAGCA53230.12688652225806044No Hit
GCTTACCATGTACTCTGCGTTGATA49030.11687481093955858No Hit
GAGTACATGGTAAGCAGTGGTATCA48960.11670794908425022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT1001.940849E-611.3956526
CGTCCGC700.00149737210.85274310
GAACAAA16800.010.463431
GTACAAA8100.010.0884651
CGTTATA856.6044315E-410.0577442
CGCAAAA2550.010.0577432
GTATCGA1054.085641E-59.95748718
AAGCGGT2300.09.90902710
ATACCGT900.0011182399.4963766
ACCGTCT900.00111836099.49626358
GTCCTAC1856.684786E-99.2451171
GTATTAT5450.09.2404051
GTATAGA4950.09.2139881
GTCCTAT2600.09.1364681
GAACCGC950.00183114868.9965676
ACCGTTC850.00746319548.9376598
GGGTAAG20950.08.929667
GTCTTAG2800.08.8232171
TATCGAC1201.721736E-48.72831619
ATGGGTA22200.08.7276485
Produced by FastQC (version 0.11.5)