##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294730_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4195087
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95752912871652	33.0	33.0	33.0	27.0	33.0
2	30.929875590184423	33.0	33.0	33.0	27.0	33.0
3	30.99747657199958	33.0	33.0	33.0	27.0	33.0
4	34.42438523920958	37.0	37.0	37.0	27.0	37.0
5	34.80244390640766	37.0	37.0	37.0	27.0	37.0
6	34.864805902714295	37.0	37.0	37.0	33.0	37.0
7	34.90178606546181	37.0	37.0	37.0	33.0	37.0
8	34.79363193182883	37.0	37.0	37.0	27.0	37.0
9	34.51100442017055	37.0	37.0	37.0	27.0	37.0
10	34.61787920012148	37.0	37.0	37.0	27.0	37.0
11	34.84331790973584	37.0	37.0	37.0	33.0	37.0
12	34.7846573861281	37.0	37.0	37.0	27.0	37.0
13	34.64996482790464	37.0	37.0	37.0	27.0	37.0
14	35.82032196233356	40.0	37.0	40.0	27.0	40.0
15	36.056494418351754	40.0	37.0	40.0	27.0	40.0
16	36.02279380618328	37.0	37.0	40.0	27.0	40.0
17	36.024058380672436	37.0	37.0	40.0	27.0	40.0
18	36.00756027228994	37.0	37.0	40.0	27.0	40.0
19	35.99368880788408	40.0	37.0	40.0	27.0	40.0
20	35.91584417677154	37.0	37.0	40.0	27.0	40.0
21	35.834846571715914	37.0	37.0	40.0	27.0	40.0
22	35.757304198935564	37.0	37.0	40.0	27.0	40.0
23	35.38960407734095	37.0	33.0	40.0	27.0	40.0
24	35.715767515667736	37.0	37.0	40.0	27.0	40.0
25	34.53789230116086	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6298340525732549
1101	2	0.6620901107822945
1101	3	0.5886749137431551
1101	4	0.7857651491024242
1101	5	0.7870892144414654
1101	6	0.5408723359691976
1101	7	0.6277654699553779
1101	8	0.5924082372327177
1101	9	0.9292639669765421
1101	10	0.9252316236441871
1101	11	0.6795322896152385
1101	12	0.728098184058112
1101	13	0.7352170305740344
1101	14	1.5126695145404057
1101	15	1.211529253381812
1101	16	1.2414916906869706
1101	17	1.2210053038851072
1101	18	1.1233021086462003
1101	19	1.1652308202181985
1101	20	1.2440106978159733
1101	21	1.177789344309609
1101	22	1.2457694520593208
1101	23	1.5818035661972019
1101	24	1.3777493080654182
1101	25	1.1992958170423051
1102	1	0.3579861940247717
1102	2	0.5614696997055333
1102	3	0.40198275280299356
1102	4	0.4690871477864249
1102	5	0.5054999760011256
1102	6	0.4098204457605519
1102	7	0.49992154102454833
1102	8	0.4941939197615568
1102	9	0.7271873038646035
1102	10	0.8256453741171441
1102	11	0.5420293997395831
1102	12	0.5416505817197219
1102	13	0.5772237111693386
1102	14	1.2832990567043865
1102	15	0.98599176717196
1102	16	0.7215484518308486
1102	17	1.0356863014732482
1102	18	0.8825992908871285
1102	19	0.9369882264252567
1102	20	0.9920396646417373
1102	21	0.9583551528339669
1102	22	1.0894598340012323
1102	23	1.4019157516300211
1102	24	1.2148623636804174
1102	25	1.081582795645879
1103	1	0.5167904746878591
1103	2	0.6099566819995772
1103	3	0.454443598852329
1103	4	0.3614020156548321
1103	5	0.5472242312600812
1103	6	0.4127685627438069
1103	7	0.45660493988327744
1103	8	0.5814829892468225
1103	9	0.7299501943348474
1103	10	0.7575892298392617
1103	11	0.5772108725006291
1103	12	0.5633721178381705
1103	13	0.4711993335251705
1103	14	1.2076916346455135
1103	15	0.9131308203853976
1103	16	0.696632546866006
1103	17	0.7859786927064363
1103	18	0.854876936637119
1103	19	0.8726143670664683
1103	20	1.0198445010799375
1103	21	0.9975489826310593
1103	22	0.9811727634904344
1103	23	1.3932493156203805
1103	24	1.0827349062392742
1103	25	1.053480860850776
1104	1	0.3379677886554653
1104	2	0.5082514456851754
1104	3	0.3803918938976949
1104	4	0.3980784690937966
1104	5	0.2721858994744153
1104	6	0.17148201375000838
1104	7	0.19993037369205524
1104	8	0.36585681265113834
1104	9	0.5352585715553033
1104	10	0.5959998386128049
1104	11	0.3642714888325571
1104	12	0.3439206966279471
1104	13	0.36788101679992735
1104	14	0.8200788809820452
1104	15	0.6450309877964742
1104	16	0.5854266612820425
1104	17	0.7157379606420946
1104	18	0.6346624945911188
1104	19	0.6107365837281407
1104	20	0.7470122774044583
1104	21	0.8098023376599173
1104	22	0.9239928186431356
1104	23	1.3038516574976526
1104	24	1.0729612632971524
1104	25	0.9903050548035068
1105	1	0.2273108094263101
1105	2	0.33344058057680215
1105	3	0.34976797361847645
1105	4	0.18993747025518815
1105	5	0.33252245743437214
1105	6	0.19386646023920662
1105	7	0.25238781993930104
1105	8	0.3148130176770607
1105	9	-1.6386150732520335
1105	10	-0.15220024519665287
1105	11	0.14633817057531928
1105	12	0.2654726962597991
1105	13	0.20708592395294545
1105	14	-1.513238028238554
1105	15	-0.16937977662898618
1105	16	-0.033102317709115425
1105	17	0.16545765449353667
1105	18	0.17963486838129938
1105	19	0.3121594387022171
1105	20	0.43908775147059487
1105	21	0.5885323524392945
1105	22	0.5421828411340996
1105	23	0.908404979348056
1105	24	0.6859771092346421
1105	25	0.41296963312874624
1106	1	0.1581255720880108
1106	2	0.13770644804559495
1106	3	0.08926159878778961
1106	4	-1.010611709645545
1106	5	-0.16100355386797816
1106	6	0.03155937023463906
1106	7	0.1343380333465518
1106	8	0.19560816269547132
1106	9	0.45992830484718183
1106	10	0.47717521196194923
1106	11	0.32630194782843347
1106	12	0.1836856430675553
1106	13	0.20923204943511564
1106	14	0.6089442296523444
1106	15	0.4270423427535448
1106	16	0.2626418566210802
1106	17	0.05195815316014318
1106	18	0.21102885075718092
1106	19	0.3372308438808176
1106	20	0.40338718289176256
1106	21	0.6382649087123582
1106	22	0.645226271724539
1106	23	0.8693181088480841
1106	24	0.5406783162664865
1106	25	0.5520011691322111
1107	1	0.29807618740777997
1107	2	0.3807480976822717
1107	3	0.39336655746321014
1107	4	0.4173996723300064
1107	5	0.4141316688111232
1107	6	0.30916932452569057
1107	7	0.3105050904457016
1107	8	0.36880417260349674
1107	9	0.6000833943220556
1107	10	0.5639321444244487
1107	11	0.2996032693555293
1107	12	0.3142698219569553
1107	13	0.25359899790808527
1107	14	0.8208484886581218
1107	15	0.5737463771006333
1107	16	0.5289856735695082
1107	17	0.6136633441748955
1107	18	0.709380142156057
1107	19	0.5552279526688437
1107	20	0.6988967407155471
1107	21	0.748339038308508
1107	22	0.6518285924661953
1107	23	0.9101807657639966
1107	24	0.8310751629984168
1107	25	0.7502037738746381
1108	1	0.49824293858514324
1108	2	0.40833211066242825
1108	3	0.46474128629298406
1108	4	0.655733688253207
1108	5	0.5823384047932265
1108	6	0.4120046912135251
1108	7	0.480416804940333
1108	8	0.4936130559511511
1108	9	0.3149547116628071
1108	10	0.5833589710502594
1108	11	0.3761900945987833
1108	12	0.46532496219194996
1108	13	0.3523976056935183
1108	14	-1.0891814809701685
1108	15	-0.004873489075919224
1108	16	0.3371247662304526
1108	17	0.5162293965035119
1108	18	0.5830071702851924
1108	19	0.5084061668944102
1108	20	0.6776871262450612
1108	21	0.6942433835202735
1108	22	0.7969660575010451
1108	23	1.077949377420282
1108	24	1.027281916396909
1108	25	0.807704682187719
1109	1	0.4031899451463623
1109	2	0.3867129603982242
1109	3	0.4381921262627202
1109	4	0.560097229913552
1109	5	0.5590814291944852
1109	6	0.3108943999227023
1109	7	0.31071968381128556
1109	8	0.3904897299399934
1109	9	0.5216129604529911
1109	10	0.44071807445467925
1109	11	0.36074415325305864
1109	12	0.3492218245381338
1109	13	0.27986543136310615
1109	14	0.679212919461655
1109	15	0.4646279221036451
1109	16	0.3900132145296382
1109	17	0.4440185275619086
1109	18	0.4469298060805613
1109	19	0.4032560462285204
1109	20	0.5923328553296017
1109	21	0.661725251312113
1109	22	0.7103090702565922
1109	23	1.0873838873619661
1109	24	0.8684731405641131
1109	25	0.5856619312509679
1110	1	0.3577849967232183
1110	2	0.1952246684432133
1110	3	0.2991325184181548
1110	4	0.4682311029073105
1110	5	0.3647308357871424
1110	6	0.1486830844281002
1110	7	0.26877400825313913
1110	8	0.2144916444058822
1110	9	0.3414438267808535
1110	10	0.4378514424153863
1110	11	0.1666274548475215
1110	12	0.16207390667135257
1110	13	0.11757941324340493
1110	14	0.5335794373033735
1110	15	0.40386034635150025
1110	16	0.295372263927689
1110	17	0.30047918734069157
1110	18	0.49198622886503074
1110	19	0.29948310028871106
1110	20	0.5247767380680486
1110	21	0.5206924115184535
1110	22	0.623543421962097
1110	23	0.8278369135285004
1110	24	0.6371061189019045
1110	25	0.6005341196065572
1111	1	0.09323432938271026
1111	2	0.029176317975895927
1111	3	0.12977721263994013
1111	4	0.4569275765260912
1111	5	0.24493469503896392
1111	6	0.16736759517655742
1111	7	0.13568807816663053
1111	8	0.2337775825955859
1111	9	0.2202267699881375
1111	10	0.09753413847531789
1111	11	0.09552957895003544
1111	12	0.047513323582712985
1111	13	0.09611499585225403
1111	14	-1.8733370209193438
1111	15	-0.6455933065887862
1111	16	-0.12684348995704653
1111	17	0.005417485798311361
1111	18	0.1160910500510397
1111	19	0.19888717448836246
1111	20	0.19868194351595037
1111	21	0.25596010473160646
1111	22	0.06712110918314806
1111	23	-2.1207639850728484
1111	24	-0.46383287045945565
1111	25	-0.017355937137111255
1112	1	-0.06374395765250895
1112	2	-0.158100175739893
1112	3	0.09351848427030873
1112	4	0.2011037236035662
1112	5	-0.4912977300375516
1112	6	-0.721797105294165
1112	7	-0.8705057737041884
1112	8	-0.5506547347606556
1112	9	-0.49407578374717787
1112	10	-0.6716063621158739
1112	11	-0.6935279536608405
1112	12	-0.6401928614093251
1112	13	-0.7285776773633259
1112	14	-0.4291058876362186
1112	15	-0.646785049734369
1112	16	-0.730731936798577
1112	17	-0.7340615050305956
1112	18	-0.8678976807513266
1112	19	-0.6837454069631406
1112	20	-0.8102448587536131
1112	21	-1.6350130002574161
1112	22	-1.4980298957280027
1112	23	-1.1120155055327956
1112	24	-1.4780081995427565
1112	25	-1.7552245842703655
1113	1	8.409034239598157E-4
1113	2	-0.1216158813296282
1113	3	-0.1665331323349264
1113	4	0.09192520607852117
1113	5	-0.04522982683535304
1113	6	-0.10526253385267381
1113	7	-0.10548044570655435
1113	8	-0.0892344661117761
1113	9	0.15585899611602372
1113	10	-0.1491242992502535
1113	11	-0.23213627763575317
1113	12	-0.18252088774161734
1113	13	-0.22391003265718723
1113	14	-0.1574029578013736
1113	15	-0.23783160704194728
1113	16	-0.22496934150296255
1113	17	-0.19850682218049087
1113	18	-0.09494985405047629
1113	19	-0.3183189434018985
1113	20	-0.8211311968786106
1113	21	-0.7450271973573805
1113	22	-0.30072045949790294
1113	23	-0.39286373179800194
1113	24	-0.19692252463037363
1113	25	-1.4760651750489018
1114	1	-0.9662601398164519
1114	2	-0.691227525843999
1114	3	-0.6297858714317002
1114	4	-1.7354058059313928
1114	5	-0.9405544947593967
1114	6	-0.6821801448237892
1114	7	-0.5904062055166435
1114	8	-0.48473469266144775
1114	9	-0.5468039354923846
1114	10	-0.5525327426037947
1114	11	-0.5160384637706343
1114	12	-0.5780018297713099
1114	13	-0.6763101909639815
1114	14	-0.635825577883125
1114	15	-1.4370939480923326
1114	16	-0.9125563824445138
1114	17	-0.9658141576644184
1114	18	-0.8621179414013582
1114	19	-0.8619246309106714
1114	20	-2.169536367075793
1114	21	-7.086055169987954
1114	22	-7.987790727424166
1114	23	-8.033327832013573
1114	24	-6.090717200435009
1114	25	-7.399163113307857
1115	1	-1.1656077037481651
1115	2	-1.1266094703148681
1115	3	-0.8899638398488534
1115	4	-0.6932872385832383
1115	5	-0.8431931977148537
1115	6	-0.9593469848873895
1115	7	-1.1118040460589498
1115	8	-0.7312645501664647
1115	9	-2.789946321642141
1115	10	-1.800180705868513
1115	11	-1.4182671407051402
1115	12	-1.2168528607536757
1115	13	-1.4085788487652096
1115	14	-1.2326751986042979
1115	15	-2.072527683320928
1115	16	-1.3606890896969759
1115	17	-3.343213380688894
1115	18	-2.8803017867947247
1115	19	-2.251078282025901
1115	20	-2.0088421749469205
1115	21	-1.5168177575014
1115	22	-1.082092716630683
1115	23	-0.5043740015143356
1115	24	-1.5661550720746007
1115	25	-1.1651531292218138
1116	1	-1.7206297719413683
1116	2	-0.9602865363954187
1116	3	-0.8292750243036444
1116	4	-0.47520810501875843
1116	5	-0.5309931102875183
1116	6	-0.5525662785405743
1116	7	-0.9837922171880322
1116	8	-1.855886047525857
1116	9	-1.2149988841897326
1116	10	-2.547389774001118
1116	11	-1.380690930349239
1116	12	-1.0184336146575959
1116	13	-0.8071273915180512
1116	14	-1.366389362198639
1116	15	-1.5190049575040518
1116	16	-1.09877788817262
1116	17	-0.9652556910408521
1116	18	-1.6122722187022518
1116	19	-1.1614976269001502
1116	20	-1.4760126700823761
1116	21	-0.6836823621594306
1116	22	-0.31737051505601244
1116	23	-0.20127920610242
1116	24	-0.3392249259998934
1116	25	-0.30168930947884576
1201	1	0.4965712678525307
1201	2	0.5613860554646379
1201	3	0.5368508524551281
1201	4	0.7147859444400737
1201	5	0.6389792961585172
1201	6	0.4980466128296541
1201	7	0.5203090636812178
1201	8	0.5680712096494958
1201	9	0.8748720109817043
1201	10	0.8197629273757201
1201	11	0.5579811924371683
1201	12	0.606802583745349
1201	13	0.6660262551912908
1201	14	1.3672290458465781
1201	15	1.0771579254175876
1201	16	1.0974529427679443
1201	17	1.0000307330910658
1201	18	0.9568613877723777
1201	19	0.9505348727067613
1201	20	1.0857361698974017
1201	21	1.1027608704082894
1201	22	1.2210395456098055
1201	23	1.0437763600246939
1201	24	1.0727942151270895
1201	25	1.0304579473144884
1202	1	-0.11273749098348063
1202	2	0.15727514654978947
1202	3	0.13878120520894655
1202	4	0.1616577942323829
1202	5	0.20917272300965806
1202	6	0.03171981334615026
1202	7	0.06851512589122422
1202	8	0.17569937548163495
1202	9	0.3035937456738935
1202	10	0.5414815128729131
1202	11	0.24116650498985592
1202	12	0.18569391124889734
1202	13	0.3400073191277002
1202	14	0.7309928951867448
1202	15	0.6394952430503409
1202	16	0.5623436429786963
1202	17	0.4340421161790786
1202	18	0.41259394114338477
1202	19	0.4726440502009126
1202	20	0.449606593428058
1202	21	0.6155902811041329
1202	22	0.5813844370507368
1202	23	0.9539629622697561
1202	24	0.6191615108740365
1202	25	0.6550067189432625
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2211	8	0.26108246022176473
2211	9	0.4130030233655333
2211	10	0.4147532961452214
2211	11	0.1266998458968729
2211	12	0.2424688359440097
2211	13	0.07318716002124859
2211	14	0.3560271938604487
2211	15	0.26731381069833304
2211	16	0.13828403987377413
2211	17	0.16844319128415464
2211	18	0.13701545624370937
2211	19	0.18145621092380537
2211	20	0.2422389384135002
2211	21	0.27652928188092574
2211	22	0.23278135656654797
2211	23	0.46327798966795797
2211	24	0.5108520512080688
2211	25	0.5599132264658735
2212	1	-0.3618783433309858
2212	2	-0.3347801063658089
2212	3	-0.29252648561830696
2212	4	-0.6655050890098977
2212	5	-0.29210785605881995
2212	6	-0.6786759225969661
2212	7	-0.6477409001092127
2212	8	-0.4718998718635703
2212	9	-0.2907040750716874
2212	10	-0.6502579625683467
2212	11	-0.5146150751016094
2212	12	-0.6568262979666315
2212	13	-0.5840894531715222
2212	14	-0.607207370601266
2212	15	-0.8033236445779863
2212	16	-0.6032299750198789
2212	17	-0.6585054811436919
2212	18	-0.5261680769307944
2212	19	-0.5811905068791106
2212	20	-0.6533995350927313
2212	21	-0.5153813749190022
2212	22	-0.6113642778055137
2212	23	-0.10032961772449056
2212	24	-0.3718251370535697
2212	25	-1.18028004440918
2213	1	-0.1653824211401691
2213	2	-1.2280242985562388
2213	3	-0.3858102148083411
2213	4	-0.037792002148954396
2213	5	-0.129226938855183
2213	6	-0.09157787448821608
2213	7	-0.009552150975011386
2213	8	0.07365158829052376
2213	9	0.022769167071786
2213	10	-0.4375223489714486
2213	11	-0.14170711395144764
2213	12	-0.06938608256412948
2213	13	-0.43008001813311836
2213	14	-0.06401982605655121
2213	15	-0.1320393012298311
2213	16	-0.15186805884653154
2213	17	-0.040872275904462185
2213	18	-0.062224406090344075
2213	19	0.00306833749979063
2213	20	0.10743531386361127
2213	21	-0.689827149684497
2213	22	-1.0517385583598795
2213	23	-0.3426576374797605
2213	24	-0.029601309725997282
2213	25	-0.6340016327516693
2214	1	-0.1869749841807078
2214	2	-0.4252061688625304
2214	3	-0.41172409715939295
2214	4	-0.6370253046085637
2214	5	-0.34321594460090665
2214	6	-0.2681456850865942
2214	7	-0.5160264449222964
2214	8	-0.09268879922151996
2214	9	-0.04691807940613302
2214	10	-2.132825399322357
2214	11	-0.9128171914832564
2214	12	-0.5740280007982079
2214	13	-0.32592061882089496
2214	14	-0.8358262330898754
2214	15	-0.5918501629008333
2214	16	-0.7101980174543527
2214	17	-0.6452689083961829
2214	18	-0.5974099291193369
2214	19	-1.489477695760968
2214	20	-2.543511313348837
2214	21	-1.880421288487959
2214	22	-2.2204860273157436
2214	23	-2.675592185179667
2214	24	-1.8345891206184106
2214	25	-2.1382496102552864
2215	1	-0.25220530096618177
2215	2	-0.38749905424223385
2215	3	-0.9898714402317772
2215	4	-0.3882932989398782
2215	5	-0.33692472146586994
2215	6	-0.2530219224466066
2215	7	-0.28277728936389934
2215	8	-0.28005174395187993
2215	9	-0.3994937594879673
2215	10	-0.3137884356892897
2215	11	-0.7562220226482168
2215	12	-0.554576008768457
2215	13	-0.43213139357608554
2215	14	-0.27431092658687817
2215	15	-0.7295570111357605
2215	16	-0.47280656949006783
2215	17	-0.34406085077170445
2215	18	-0.3925747683824099
2215	19	-2.57516827317788
2215	20	-2.696962602121225
2215	21	-3.654178067995467
2215	22	-2.7375877012813703
2215	23	-3.8398869565677494
2215	24	-2.763267902491421
2215	25	-2.0390932527021945
2216	1	-1.7705799835052076
2216	2	-1.382368112792932
2216	3	-0.9927483742964078
2216	4	-1.1894185102358392
2216	5	-2.164699419998584
2216	6	-1.7765449188921494
2216	7	-1.3579687310848172
2216	8	-5.79909664485994
2216	9	-4.525948978532593
2216	10	-2.3802835838101686
2216	11	-1.3851035948831694
2216	12	-1.3435287805026377
2216	13	-2.2090360904818596
2216	14	-3.896518028337084
2216	15	-2.455538348153162
2216	16	-3.4173208714344057
2216	17	-3.116580105245113
2216	18	-3.0831062276963905
2216	19	-1.941102952738774
2216	20	-1.1300521695470422
2216	21	-1.0422197508626425
2216	22	-0.8948430460694965
2216	23	-1.2156293533601854
2216	24	-0.6947634780298984
2216	25	-0.6952502721400435
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	55.0
8	334.0
9	879.0
10	1848.0
11	3431.0
12	5938.0
13	8807.0
14	12215.0
15	16803.0
16	21140.0
17	23272.0
18	24751.0
19	25710.0
20	26404.0
21	28677.0
22	30187.0
23	32208.0
24	34081.0
25	36811.0
26	39040.0
27	43863.0
28	49705.0
29	58214.0
30	70725.0
31	86655.0
32	115915.0
33	163464.0
34	235350.0
35	370149.0
36	636077.0
37	1992376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.887414723478734	19.061046185835455	11.280822946746436	24.770716143939374
2	13.344170040620964	22.25157627361907	37.1531137096109	27.25113997614907
3	20.494028262544248	28.364542902736726	27.27002438859818	23.871404446120845
4	13.020194299017158	18.91396930107955	33.79208298026253	34.27375341964077
5	11.882004194857497	39.87455959837708	35.20127757897068	13.042158627794734
6	32.55036252915788	33.706355553913326	18.31690638606763	15.426375530861167
7	25.02232382296362	33.14807619419938	23.157158283413626	18.67244169942337
8	26.762199438865142	35.76073819983934	21.34498351636564	16.132078844929882
9	26.885593381949107	17.03491620644413	20.04111014066028	36.038380270946476
10	15.913454788336526	31.266443373295232	32.538815121240866	20.28128671712738
11	30.204820015213173	26.91582386761156	20.41628119242469	22.463074924750583
12	23.322166209143532	29.643565800853004	29.09651656284594	17.937751427157515
13	28.65273988864807	22.25062383968679	23.355418418360195	25.741217853304942
14	21.24538832689203	25.54925899766416	25.837717258461794	27.36763541698202
15	29.70499179738174	25.807162904809104	21.711264976750904	22.776580321058248
16	19.88160647705491	28.740071795113842	30.023864163778104	21.35445756405315
17	24.981609049634827	29.25836913390662	27.11779600371681	18.642225812741746
18	25.542202794727302	26.24313731847014	27.97080122842327	20.24385865837928
19	22.874617795321125	30.541905270211288	28.549160931560362	18.03431600290722
20	23.647478840058326	27.6660263122954	27.061197743162037	21.625297104484236
21	20.238010036843747	29.135307494043065	29.54697267684474	21.079709792268442
22	21.98711937397018	28.57963151113451	23.404980632780973	26.028268482114335
23	19.17308389633192	29.636412649337764	27.119260277109813	24.071243177220506
24	21.38490415095563	33.502898903799114	24.668214846411257	20.443982098834002
25	22.37100981004198	26.36290202931043	23.196696108021555	28.069392052626036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9282.0
1	9282.0
2	11617.5
3	13953.0
4	13953.0
5	13953.0
6	16898.5
7	19844.0
8	19844.0
9	19844.0
10	20907.0
11	21970.0
12	21970.0
13	21970.0
14	27490.0
15	33010.0
16	33010.0
17	33010.0
18	46197.0
19	59384.0
20	59384.0
21	59384.0
22	81382.0
23	103380.0
24	103380.0
25	103380.0
26	132597.0
27	161814.0
28	161814.0
29	161814.0
30	193882.0
31	225950.0
32	225950.0
33	225950.0
34	254645.0
35	283340.0
36	283340.0
37	283340.0
38	310206.5
39	337073.0
40	337073.0
41	337073.0
42	386211.0
43	435349.0
44	435349.0
45	435349.0
46	679157.5
47	922966.0
48	922966.0
49	922966.0
50	803667.5
51	684369.0
52	684369.0
53	684369.0
54	517715.0
55	351061.0
56	351061.0
57	351061.0
58	284175.5
59	217290.0
60	217290.0
61	217290.0
62	183208.0
63	149126.0
64	149126.0
65	149126.0
66	118821.5
67	88517.0
68	88517.0
69	88517.0
70	66866.0
71	45215.0
72	45215.0
73	45215.0
74	32711.5
75	20208.0
76	20208.0
77	20208.0
78	14101.5
79	7995.0
80	7995.0
81	7995.0
82	5392.5
83	2790.0
84	2790.0
85	2790.0
86	1837.0
87	884.0
88	884.0
89	884.0
90	564.5
91	245.0
92	245.0
93	245.0
94	149.5
95	54.0
96	54.0
97	54.0
98	36.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032824110679945374
2	0.016710022938737624
3	0.021787390821692138
4	0.02224030157181484
5	0.02154901674268019
6	0.006007026791101115
7	4.052359343203133E-4
8	4.052359343203133E-4
9	3.5756111851792345E-4
10	1.9069926320955917E-4
11	9.058215002454061E-4
12	8.343092765418213E-4
13	0.0023599033822182948
14	6.197726054310674E-4
15	0.0020261796716015664
16	0.0022407163427123206
17	0.002121529303206346
18	0.002765139316538608
19	4.2907334222150816E-4
20	3.3372371061672856E-4
21	0.0794024057188802
22	0.07651807936283563
23	0.07740006345517983
24	0.0012633826187633296
25	0.22593095208752523
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4195087.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.31857482431161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.64044611762765	43.236303637195945
2	11.216697732506338	11.736832791996386
3	2.994174587749017	4.69952841618598
4	1.1689321338538556	2.4462745323830073
5	0.570680814206679	1.4928603439435608
6	0.336443098243742	1.0561334045753066
7	0.2148596065702915	0.7868803882148967
8	0.14220115253874452	0.5951809311361324
9	0.11057740810464	0.5206727159820944
>10	0.5417415627426332	5.089123394247839
>50	0.03633018426726521	1.2858594591034718
>100	0.0198912191324552	2.002793466828113
>500	0.0023568651699984538	0.8735513289534644
>1k	0.0029114216739236846	3.1691803861918193
>5k	5.545565093187971E-4	2.2407449869244735
>10k+	0.0012015391035240604	18.768079816137497
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	136053	3.243150857181269	No Hit
CCCATGTACTCTGCGTTGATACCAC	131746	3.1404831413508227	No Hit
GAGTACATGGGAAGCAGTGGTATCA	59572	1.4200420634899824	No Hit
CATGTACTCTGCGTTGATACCACTG	48919	1.1661021571185533	No Hit
GCGTTGATACCACTGCTTCCCATGT	41636	0.9924943153741508	No Hit
GTATCAACGCAGAGTACATGGGAAG	32703	0.7795547505927768	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	31287	0.7458009810046848	No Hit
GCTTCCCATGTACTCTGCGTTGATA	30060	0.7165524815099187	No Hit
TATCAACGCAGAGTACATGGGAAGC	26697	0.6363872787382002	No Hit
ACTCTGCGTTGATACCACTGCTTCC	26351	0.6281395356043867	No Hit
GGTATCAACGCAGAGTACATGGGAA	20606	0.4911936272120221	No Hit
CAGTGGTATCAACGCAGAGTACATG	17398	0.4147232226649888	No Hit
GCAGTGGTATCAACGCAGAGTACAT	14939	0.35610703663595056	No Hit
GTGGTATCAACGCAGAGTACATGGG	14796	0.3526982873060797	No Hit
GTACATGGTAAGCAGTGGTATCAAC	14480	0.34516566640930213	No Hit
GTACTCTGCGTTGATACCACTGCTT	14249	0.3396592251841261	No Hit
ATACCACTGCTTCCCATGTACTCTG	14213	0.3388010784996831	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	12514	0.2983013224755529	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	12481	0.2975146880148135	No Hit
CATGGGAAGCAGTGGTATCAACGCA	11526	0.2747499634691724	No Hit
TACCACTGCTTCCCATGTACTCTGC	11460	0.2731766945476935	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	10863	0.2589457620306802	No Hit
ACCATGTACTCTGCGTTGATACCAC	10720	0.2555370127008093	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10617	0.25308175968698626	No Hit
ACATGGGAAGCAGTGGTATCAACGC	10302	0.24557297619810983	No Hit
GTTGATACCACTGCTTCCCATGTAC	10058	0.2397566486702183	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9885	0.23563277710331154	No Hit
CTTCCCATGTACTCTGCGTTGATAC	9748	0.23236705222084786	No Hit
CCATGTACTCTGCGTTGATACCACT	9661	0.2302931977334439	No Hit
GATACCACTGCTTCCCATGTACTCT	9517	0.22686061099567184	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7816	0.18631318015573933	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	7807	0.18609864348462857	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7176	0.17105723909897458	No Hit
TTCCCATGTACTCTGCGTTGATACC	6753	0.16097401555676916	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6739	0.16064029184615242	No Hit
TGATACCACTGCTTCCCATGTACTC	6620	0.15780364030591024	No Hit
CCCCATGTACTCTGCGTTGATACCA	5632	0.13425228129952965	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5323	0.12688652225806044	No Hit
GCTTACCATGTACTCTGCGTTGATA	4903	0.11687481093955858	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4896	0.11670794908425022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.38374079011949E-5	0.0
8	0.0	0.0	0.0	2.38374079011949E-5	0.0
9	0.0	0.0	0.0	7.15122237035847E-5	0.0
10	0.0	2.38374079011949E-5	0.0	1.1918703950597448E-4	0.0
11	0.0	2.38374079011949E-5	0.0	1.430244474071694E-4	0.0
12	0.0	2.38374079011949E-5	0.0	1.6686185530836428E-4	0.0
13	0.0	2.38374079011949E-5	0.0	2.1453667111075408E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	100	1.940849E-6	11.395652	6
CGTCCGC	70	0.001497372	10.852743	10
GAACAAA	1680	0.0	10.46343	1
GTACAAA	810	0.0	10.088465	1
CGTTATA	85	6.6044315E-4	10.057744	2
CGCAAAA	255	0.0	10.057743	2
GTATCGA	105	4.085641E-5	9.957487	18
AAGCGGT	230	0.0	9.909027	10
ATACCGT	90	0.001118239	9.496376	6
ACCGTCT	90	0.0011183609	9.4962635	8
GTCCTAC	185	6.684786E-9	9.245117	1
GTATTAT	545	0.0	9.240405	1
GTATAGA	495	0.0	9.213988	1
GTCCTAT	260	0.0	9.136468	1
GAACCGC	95	0.0018311486	8.996567	6
ACCGTTC	85	0.0074631954	8.937659	8
GGGTAAG	2095	0.0	8.92966	7
GTCTTAG	280	0.0	8.823217	1
TATCGAC	120	1.721736E-4	8.728316	19
ATGGGTA	2220	0.0	8.727648	5
>>END_MODULE