FastQCFastQC Report
Wed 25 May 2016
SRR1294730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195087
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1443203.4402147083004477No Hit
GTACATGGGAAGCAGTGGTATCAAC1323283.1543565127493185No Hit
GAGTACATGGGAAGCAGTGGTATCA639101.523448738965366No Hit
CATGTACTCTGCGTTGATACCACTG567661.3531542969192296No Hit
GCGTTGATACCACTGCTTCCCATGT461671.100501610574465No Hit
ACGCAGAGTACATGGGAAGCAGTGG374010.8915428929125904No Hit
GCTTCCCATGTACTCTGCGTTGATA349860.8339755528312047No Hit
GTATCAACGCAGAGTACATGGGAAG347520.828397599382325No Hit
TATCAACGCAGAGTACATGGGAAGC302620.72136763790596No Hit
ACTCTGCGTTGATACCACTGCTTCC301400.7184594741420142No Hit
GGTATCAACGCAGAGTACATGGGAA229580.5472592105956324No Hit
CAGTGGTATCAACGCAGAGTACATG208090.49603262101596457No Hit
GCAGTGGTATCAACGCAGAGTACAT168550.40177951017464No Hit
GTGGTATCAACGCAGAGTACATGGG165230.39386549075144334No Hit
ATACCACTGCTTCCCATGTACTCTG165180.3937463037119373No Hit
CATGGGAAGCAGTGGTATCAACGCA146720.34974244872633153No Hit
GTACTCTGCGTTGATACCACTGCTT146030.3480976675811491No Hit
GGGAAGCAGTGGTATCAACGCAGAG145230.3461906749490535No Hit
CTGCTTCCCATGTACTCTGCGTTGA143140.3412086566977038No Hit
GTACATGGTAAGCAGTGGTATCAAC139210.33184055539253415No Hit
ACATGGGAAGCAGTGGTATCAACGC130750.3116741083081233No Hit
TACCACTGCTTCCCATGTACTCTGC129150.3078601230439321No Hit
AAGCAGTGGTATCAACGCAGAGTAC127050.3028542673846812No Hit
GCAGAGTACATGGGAAGCAGTGGTA123170.2936053531190176No Hit
ACCATGTACTCTGCGTTGATACCAC119660.2852384229456982No Hit
CCATGTACTCTGCGTTGATACCACT116160.2768953301802799No Hit
CTTCCCATGTACTCTGCGTTGATAC114130.27205633637633736No Hit
GATACCACTGCTTCCCATGTACTCT111660.26616849662474223No Hit
CCACTGCTTCCCATGTACTCTGCGT111310.2653341873482004No Hit
GTTGATACCACTGCTTCCCATGTAC108190.2578969160830276No Hit
GTATCAACGCAGAGTACTTTTTTTT100270.23901768902528123No Hit
ATGGGAAGCAGTGGTATCAACGCAG96390.2297687747596176No Hit
GTACATGGGTAAGCAGTGGTATCAA78930.18814866056413132No Hit
GGTAAGCAGTGGTATCAACGCAGAG78890.18805331093252653No Hit
TATCAACGCAGAGTACTTTTTTTTT77170.18395327677352102No Hit
TTCCCATGTACTCTGCGTTGATACC73640.17553867178439922No Hit
TGATACCACTGCTTCCCATGTACTC72060.17177236133601043No Hit
GGTATCAACGCAGAGTACTTTTTTT70630.16836361200613956No Hit
CCCCATGTACTCTGCGTTGATACCA64190.15301232131777004No Hit
ATCAACGCAGAGTACATGGGAAGCA63150.15053323089604578No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58340.13906743769557103No Hit
GCTTACCATGTACTCTGCGTTGATA56660.1350627531681703No Hit
GAGTACATGGTAAGCAGTGGTATCA54460.1298185234299074No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51440.12261962624374656No Hit
GGAAGCAGTGGTATCAACGCAGAGT45480.1084125311346344No Hit
AAAAAGTACTCTGCGTTGATACCAC43090.10271539064624882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGC707.3142437E-613.5633836
CGCAAAA1501.8189894E-1212.6878922
TATACCG701.0749411E-412.2346075
TACGTCG701.0749411E-412.2346075
ACGCAAA1900.012.0268611
TAACGCA1201.4933576E-610.3087895
TGGACCG1303.759851E-710.247795
ATTAACG1409.4702045E-810.1955063
GGACCGT1054.1355874E-59.9464816
TTAACGC1252.5188E-69.8964384
TTACACT4800.09.7140514
CCCCTAG1502.5670488E-79.5212651
TAGACTG3100.09.5158055
ATACCGT1201.7140132E-59.4943686
TATCGGT1304.279167E-69.49368913
CTAGACA2700.09.1633684
TATTAGG2600.09.1499212
CTGTGCG2002.6921043E-99.0192199
AGTACTC13650.08.9929595
TTAGACT2553.6379788E-128.9560524
Produced by FastQC (version 0.11.5)