FastQCFastQC Report
Wed 25 May 2016
SRR1294729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780003
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1641443.4339727401844726No Hit
GTACATGGGAAGCAGTGGTATCAAC1635903.4223827892995042No Hit
GAGTACATGGGAAGCAGTGGTATCA774021.6192876866395274No Hit
CATGTACTCTGCGTTGATACCACTG651531.3630326173435456No Hit
GCGTTGATACCACTGCTTCCCATGT536261.121882141078154No Hit
ACGCAGAGTACATGGGAAGCAGTGG434720.9094554961576384No Hit
GCTTCCCATGTACTCTGCGTTGATA418570.87566890648395No Hit
GTATCAACGCAGAGTACATGGGAAG405340.847991099587176No Hit
ACTCTGCGTTGATACCACTGCTTCC367760.7693719020678439No Hit
TATCAACGCAGAGTACATGGGAAGC331730.6939953803376274No Hit
GGTATCAACGCAGAGTACATGGGAA275960.5773218133963514No Hit
CAGTGGTATCAACGCAGAGTACATG232240.48585743565432904No Hit
GTGGTATCAACGCAGAGTACATGGG210450.44027169020605217No Hit
GCAGTGGTATCAACGCAGAGTACAT188450.3942466144895725No Hit
ATACCACTGCTTCCCATGTACTCTG185670.38843071855812644No Hit
GTACATGGTAAGCAGTGGTATCAAC184580.38615038526126444No Hit
GTACTCTGCGTTGATACCACTGCTT171660.3591211135223137No Hit
CATGGGAAGCAGTGGTATCAACGCA161200.3372382820680238No Hit
ACATGGGAAGCAGTGGTATCAACGC159720.33414204970164246No Hit
CTGCTTCCCATGTACTCTGCGTTGA159460.3335981169886295No Hit
GGGAAGCAGTGGTATCAACGCAGAG150500.314853358878645No Hit
TACCACTGCTTCCCATGTACTCTGC143440.3000834936714475No Hit
ACCATGTACTCTGCGTTGATACCAC136600.28577387922141473No Hit
CTTCCCATGTACTCTGCGTTGATAC135780.28405839912652775No Hit
GTTGATACCACTGCTTCCCATGTAC134910.2822383165868306No Hit
GATACCACTGCTTCCCATGTACTCT133930.28018810866855104No Hit
GCAGAGTACATGGGAAGCAGTGGTA131000.27405840540267445No Hit
AAGCAGTGGTATCAACGCAGAGTAC130410.27282409655391426No Hit
CCACTGCTTCCCATGTACTCTGCGT126720.2651044361269229No Hit
CCATGTACTCTGCGTTGATACCACT125070.26165255544818694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA115390.24140152213293592No Hit
TTCCCATGTACTCTGCGTTGATACC105450.22060655610467192No Hit
ATGGGAAGCAGTGGTATCAACGCAG103720.2169873115142396No Hit
GTACATGGGTAAGCAGTGGTATCAA98240.20552288356304377No Hit
TGATACCACTGCTTCCCATGTACTC94110.19688272162172285No Hit
GGTAAGCAGTGGTATCAACGCAGAG79540.16640156920403606No Hit
CCCCATGTACTCTGCGTTGATACCA69940.1463178998004813No Hit
ATCAACGCAGAGTACATGGGAAGCA66770.13968610479951582No Hit
GCTTACCATGTACTCTGCGTTGATA66360.13882836475207233No Hit
GAGTACATGGTAAGCAGTGGTATCA62280.13029280525556156No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA55190.11546017858147788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT2600.011.3284891
GAACAAA20950.011.2020251
GTATACG600.00587263711.0848661
CCTACAC4700.010.5101123
GTATAGG4150.010.0736821
TATCGAC1909.640644E-1110.0202619
CGCAAAA4400.09.715932
GTATCGA2106.548362E-119.50479518
GTCCTAA2800.09.5013131
TAGGACC1804.1964086E-99.4992264
ACGCAAA5550.09.4157161
GTGTAGC4050.09.3840131
CCTAGCC2357.2759576E-129.2974073
TACGTGG3100.09.1935692
GTACTAG2304.1836756E-119.0882131
AGGACCG1151.1133203E-49.0856465
GGTATCG2106.6211214E-109.05218617
CTACACT5800.09.0078874
TAGACTG3800.08.9987015
CCGTGCG850.0074649148.9374559
Produced by FastQC (version 0.11.5)