##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294729_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4780003
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.719482393630297	33.0	33.0	33.0	27.0	33.0
2	30.673215058651635	33.0	33.0	33.0	27.0	33.0
3	30.707345790368752	33.0	33.0	33.0	27.0	33.0
4	34.04903239600477	37.0	33.0	37.0	27.0	37.0
5	34.412820452204734	37.0	33.0	37.0	27.0	37.0
6	34.57317851055742	37.0	37.0	37.0	27.0	37.0
7	34.57208206773092	37.0	37.0	37.0	27.0	37.0
8	34.48447354530949	37.0	37.0	37.0	27.0	37.0
9	34.19987121346995	37.0	37.0	37.0	27.0	37.0
10	34.28903642947505	37.0	37.0	37.0	27.0	37.0
11	34.564179980640176	37.0	37.0	37.0	27.0	37.0
12	34.50263692303122	37.0	37.0	37.0	27.0	37.0
13	34.3585732059164	37.0	37.0	37.0	27.0	37.0
14	35.43539575184367	37.0	33.0	40.0	27.0	40.0
15	35.674245601937905	37.0	37.0	40.0	27.0	40.0
16	35.621725551218276	37.0	37.0	40.0	27.0	40.0
17	35.62311404407068	37.0	37.0	40.0	27.0	40.0
18	35.58846532104687	37.0	37.0	40.0	27.0	40.0
19	35.55352684925093	37.0	33.0	40.0	27.0	40.0
20	35.498551988356496	37.0	33.0	40.0	27.0	40.0
21	35.40905873908447	37.0	33.0	40.0	27.0	40.0
22	35.33042594324731	37.0	33.0	40.0	27.0	40.0
23	34.94963643328257	37.0	33.0	40.0	22.0	40.0
24	35.300600648158586	37.0	33.0	40.0	27.0	40.0
25	34.040369430730486	37.0	33.0	40.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5400540910312017
1101	2	0.6248687082202622
1101	3	0.4555786518376266
1101	4	0.6205878347584459
1101	5	0.5183600426220778
1101	6	0.4782155577840186
1101	7	0.4300441543072253
1101	8	0.5040889852996955
1101	9	0.7198122883589306
1101	10	0.7436242958557244
1101	11	0.5566776508913946
1101	12	0.5746664977135083
1101	13	0.5653398693948049
1101	14	1.2869155828779455
1101	15	1.0453400828992088
1101	16	0.9698629936731464
1101	17	1.0583149559763996
1101	18	0.9976605345919012
1101	19	0.9570521061061541
1101	20	1.0610808057930967
1101	21	1.0566259168387333
1101	22	1.0797077791917076
1101	23	1.5287309993718452
1101	24	1.293992701289028
1101	25	1.1618224178707166
1102	1	0.3999858708598545
1102	2	0.5584975345743892
1102	3	0.32247788814988354
1102	4	0.3944534503054342
1102	5	0.37970688882764136
1102	6	0.353226306083009
1102	7	0.31684082233560673
1102	8	0.4046532594396197
1102	9	0.6170213085486154
1102	10	0.7194299957665748
1102	11	0.42693399032361157
1102	12	0.4915980310650383
1102	13	0.49900679724594
1102	14	1.123755382790037
1102	15	0.8919788696770112
1102	16	0.6365873501346684
1102	17	0.8553629672384417
1102	18	0.7959753131197473
1102	19	0.9082320473279353
1102	20	0.8945090165844292
1102	21	0.8769744341561747
1102	22	0.9938590858698575
1102	23	1.265680435696538
1102	24	1.0468664991755148
1102	25	1.0313928013448717
1103	1	0.38205136773947146
1103	2	0.6437608784481093
1103	3	0.3891770673952948
1103	4	0.3811887003890604
1103	5	0.4116246982122007
1103	6	0.43543831300176805
1103	7	0.3630396925450725
1103	8	0.5274452675839214
1103	9	0.6830682018712366
1103	10	0.7136590438631174
1103	11	0.6159226321275852
1103	12	0.6291577407179716
1103	13	0.6323213775901735
1103	14	1.2987549235205833
1103	15	1.0195930838262868
1103	16	0.6782341510855616
1103	17	0.7581160194692771
1103	18	0.9118567029911873
1103	19	0.9740393191231007
1103	20	1.0795730929960143
1103	21	1.0909424643167966
1103	22	0.9627222195029717
1103	23	1.452999062790326
1103	24	1.1091636577339443
1103	25	0.9594035777173886
1104	1	0.2534611008125509
1104	2	0.44010232120437465
1104	3	0.2564341479600927
1104	4	0.20566844973633636
1104	5	0.18133238058355516
1104	6	0.13755593912084407
1104	7	0.2025489184481799
1104	8	0.36770843671747855
1104	9	0.587127732846966
1104	10	0.5842543512345628
1104	11	0.2448520542134034
1104	12	0.2573384685451785
1104	13	0.3577617365053243
1104	14	0.7858875461908426
1104	15	0.5431371085040908
1104	16	0.6035009599039327
1104	17	0.6680846998085599
1104	18	0.5424817815766474
1104	19	0.652294880587732
1104	20	0.6791669657280082
1104	21	0.6574304750686011
1104	22	0.7790874162427883
1104	23	1.201744618163758
1104	24	0.9366115813719063
1104	25	0.8422830300682946
1105	1	0.20324660986466725
1105	2	0.28033477073030966
1105	3	0.27632534169875456
1105	4	0.21699274868479534
1105	5	0.22229540191923292
1105	6	0.10922257221162823
1105	7	0.16347468744669413
1105	8	0.20959352624392835
1105	9	-1.7486258125201886
1105	10	-0.2330505064565358
1105	11	0.014892346443694748
1105	12	0.1486640563360666
1105	13	0.19683956856868434
1105	14	-1.5992595501182407
1105	15	-0.2176847598454117
1105	16	-0.15256860632833735
1105	17	0.21253947488926173
1105	18	0.14601684623927014
1105	19	0.3093553907187854
1105	20	0.41799516212890353
1105	21	0.5977013267582194
1105	22	0.557962071447001
1105	23	0.9052862958996641
1105	24	0.6847579475332921
1105	25	0.24996947504699563
1106	1	0.08706297373009875
1106	2	0.17725973452236232
1106	3	0.17103955374101076
1106	4	-1.1446633160761621
1106	5	-0.2511655828157373
1106	6	-0.12966705794625
1106	7	0.04139599697834484
1106	8	0.18863362771759995
1106	9	0.4831598329648159
1106	10	0.516936520786409
1106	11	0.4106130306943925
1106	12	0.1546969721887237
1106	13	0.30413556983655354
1106	14	0.7640757890612733
1106	15	0.4889079299792769
1106	16	0.4098762891401364
1106	17	0.11198711052858812
1106	18	0.28716863628381617
1106	19	0.2839509721917963
1106	20	0.3941245371095832
1106	21	0.5876641136476266
1106	22	0.6680260494037285
1106	23	0.8340026088358954
1106	24	0.5381483177221469
1106	25	0.6081239047259999
1107	1	0.38373756070702214
1107	2	0.2174362183406231
1107	3	0.2965313408776602
1107	4	0.5045194579666372
1107	5	0.36120686954703984
1107	6	0.21306497492773246
1107	7	0.22373902204876828
1107	8	0.35076560034902116
1107	9	0.4748125774713188
1107	10	0.5996634922678439
1107	11	0.3290020726523508
1107	12	0.3358340105728317
1107	13	0.23917157003566558
1107	14	0.8889945815067506
1107	15	0.6238002636591204
1107	16	0.6067411143513226
1107	17	0.5175793770259887
1107	18	0.59101587597182
1107	19	0.5013313327533382
1107	20	0.7123948834171117
1107	21	0.6874125155665212
1107	22	0.5940686762846212
1107	23	0.887130289638975
1107	24	0.7844422919762621
1107	25	0.7637200317615012
1108	1	0.557925267444034
1108	2	0.4740910352342276
1108	3	0.6443931138365535
1108	4	0.7978630836840139
1108	5	0.6462925463678815
1108	6	0.4629978284831253
1108	7	0.5001223834831876
1108	8	0.6069777953688487
1108	9	0.31164097260491275
1108	10	0.6089173616561752
1108	11	0.4715156781049501
1108	12	0.47551482752294305
1108	13	0.453017464775229
1108	14	-0.9403237944241454
1108	15	0.15714868579426167
1108	16	0.37221475294651896
1108	17	0.5306399562249169
1108	18	0.6662314679719756
1108	19	0.7350163636747453
1108	20	0.8595333810864716
1108	21	0.8768375717942263
1108	22	0.9303996856190864
1108	23	1.3178262655159543
1108	24	1.1451849912258893
1108	25	1.0841635455170788
1109	1	0.3971291197150393
1109	2	0.35776953377447285
1109	3	0.4230424120470282
1109	4	0.5363456293106381
1109	5	0.4877524309981638
1109	6	0.31275159884502557
1109	7	0.307641423923279
1109	8	0.37435247820259576
1109	9	0.616034239293306
1109	10	0.5042954568984612
1109	11	0.41559615184034726
1109	12	0.34819592702005764
1109	13	0.24877443280503542
1109	14	0.7403790813630522
1109	15	0.4677810467041823
1109	16	0.4929870992097065
1109	17	0.5051789597991174
1109	18	0.45247841927253063
1109	19	0.4867760961238332
1109	20	0.6361066439530703
1109	21	0.5489467412398525
1109	22	0.6982360817272166
1109	23	1.049564783433084
1109	24	0.8774821277873244
1109	25	0.6388046743746401
1110	1	0.3947732417516363
1110	2	0.1957002794795315
1110	3	0.37993558796457094
1110	4	0.5311761595628326
1110	5	0.4673835037420986
1110	6	0.24027671766536685
1110	7	0.2141423219803471
1110	8	0.34629490623959214
1110	9	0.43944208000101526
1110	10	0.41895388634642927
1110	11	0.2112632170963593
1110	12	0.19770826211547643
1110	13	0.1395748774238541
1110	14	0.6795380201611323
1110	15	0.4106932354384938
1110	16	0.32437436772216444
1110	17	0.4107684544824437
1110	18	0.4859683798438681
1110	19	0.3437786472382385
1110	20	0.4595280856687438
1110	21	0.5166909575258387
1110	22	0.5290592055598609
1110	23	0.65008095272227
1110	24	0.6597910335405572
1110	25	0.5460375647565954
1111	1	0.1836959989184983
1111	2	0.11499945747809193
1111	3	0.33642778774167326
1111	4	0.4936028831302579
1111	5	0.3813792575378372
1111	6	0.3405389577936404
1111	7	0.27722516412958953
1111	8	0.35874864734066847
1111	9	0.3410464150285222
1111	10	0.3293527278256789
1111	11	0.2870762062789751
1111	12	0.2960622205767436
1111	13	0.2502360883919792
1111	14	-1.9717673813593422
1111	15	-0.5026790494074476
1111	16	-0.019687696806471422
1111	17	0.2180303686383951
1111	18	0.29890265789818216
1111	19	0.3691662701244596
1111	20	0.35429191423870776
1111	21	0.5083811930654463
1111	22	0.4072770770013321
1111	23	-1.6225293759419088
1111	24	-0.24873296352960494
1111	25	0.20370393791662167
1112	1	-0.17372175272561563
1112	2	-0.27262661828558876
1112	3	-0.03378308804047592
1112	4	0.1666700940587802
1112	5	-0.6883098185946395
1112	6	-0.8060795963883862
1112	7	-0.9218021122193463
1112	8	-0.6806472285833323
1112	9	-0.5080466363334892
1112	10	-0.7185315323292798
1112	11	-0.831294212055866
1112	12	-0.7498961403596454
1112	13	-0.8553509529470276
1112	14	-0.5460074472373648
1112	15	-0.8682184015873773
1112	16	-0.8544433823928372
1112	17	-0.842023103614082
1112	18	-1.095060310335107
1112	19	-0.9003172900464449
1112	20	-1.0021413443966054
1112	21	-1.8794769153171131
1112	22	-1.8183553879191194
1112	23	-1.4829695407439303
1112	24	-1.7434509381190253
1112	25	-1.9968073408323264
1113	1	6.837688084004867E-4
1113	2	-0.20206052281142206
1113	3	-0.04926693616368638
1113	4	0.10132764876147604
1113	5	0.05915258859068473
1113	6	-0.1655948097405897
1113	7	-0.08070458256745638
1113	8	0.035757013220269585
1113	9	0.1616672510093835
1113	10	-0.14483254137221024
1113	11	-0.11216305810381755
1113	12	-0.0830749788997096
1113	13	-0.3492382148711499
1113	14	-0.06869854621458416
1113	15	-0.21904800633890886
1113	16	-0.16407611447204573
1113	17	-0.11200514172117693
1113	18	-0.09540208777367809
1113	19	-0.14232897817672097
1113	20	-0.7202798251435922
1113	21	-0.5936873269365393
1113	22	-0.10895896628858281
1113	23	-0.37889624826156876
1113	24	-0.08045503292882472
1113	25	-1.185997567607366
1114	1	-1.026161718143804
1114	2	-0.7793649691779159
1114	3	-0.6023781290074517
1114	4	-1.7202590280420438
1114	5	-0.9261805896667781
1114	6	-0.6995074809444901
1114	7	-0.5281996402102749
1114	8	-0.4828241273564444
1114	9	-0.5975313090538421
1114	10	-0.5945974590207328
1114	11	-0.6017746470283853
1114	12	-0.6643519737879657
1114	13	-0.7064245771032276
1114	14	-0.7477910785399899
1114	15	-1.4578597108628983
1114	16	-1.032921465966659
1114	17	-0.988690261460718
1114	18	-0.9349572202792302
1114	19	-0.911848876155986
1114	20	-2.1607520683673656
1114	21	-6.836697576428868
1114	22	-7.804600485608017
1114	23	-7.865864325249543
1114	24	-5.8600549335282
1114	25	-7.22066664535005
1115	1	-1.1125650810903274
1115	2	-1.086412014972563
1115	3	-0.7778829204216464
1115	4	-0.6796808084096426
1115	5	-0.7304717130493827
1115	6	-0.9222804917740675
1115	7	-1.0863393622275908
1115	8	-0.7982272474346814
1115	9	-2.799033889636892
1115	10	-1.7164294596272498
1115	11	-1.2268328504869928
1115	12	-1.032453081310038
1115	13	-1.4969191504264288
1115	14	-1.230768612443157
1115	15	-2.135899449926569
1115	16	-1.503096979649328
1115	17	-3.1663960845217574
1115	18	-2.78081945191591
1115	19	-2.2385243701281183
1115	20	-1.8946692469371271
1115	21	-1.307887601371867
1115	22	-0.8813938683731095
1115	23	-0.3649668503052723
1115	24	-1.2960743902700003
1115	25	-1.02889568623322
1116	1	-1.657803792676436
1116	2	-1.0047658187643336
1116	3	-0.7456812790868845
1116	4	-0.3478567187556578
1116	5	-0.3145495947845234
1116	6	-0.4685863714259568
1116	7	-0.7464413832327281
1116	8	-1.9593582229286852
1116	9	-0.9148868998805995
1116	10	-2.5454874949563155
1116	11	-1.3417621091436018
1116	12	-0.9371886757160652
1116	13	-0.8473425429908019
1116	14	-1.2470551464717303
1116	15	-1.2379633297756882
1116	16	-0.8701254040788982
1116	17	-0.7966075766386638
1116	18	-1.4110714689147912
1116	19	-0.8441088683591929
1116	20	-1.2200627851445347
1116	21	-0.5950556594441778
1116	22	-0.18859797509993115
1116	23	-0.13251065724042377
1116	24	-0.20520828263098423
1116	25	-0.17360899965851218
1201	1	0.32734767231280415
1201	2	0.3877114545899687
1201	3	0.28347124808852797
1201	4	0.40667809198208005
1201	5	0.43268596224245925
1201	6	0.33641814631393174
1201	7	0.3951235480700319
1201	8	0.46671241245176986
1201	9	0.7054212030824871
1201	10	0.6808712756666466
1201	11	0.4737364975992122
1201	12	0.4882715474809842
1201	13	0.5783608104826854
1201	14	1.3303582232794184
1201	15	0.9833012580408962
1201	16	0.9279528321402708
1201	17	0.86908087863916
1201	18	0.6909430637232958
1201	19	0.7649559249973095
1201	20	0.8972136039742651
1201	21	0.887800897141382
1201	22	0.9462482837976012
1201	23	0.7505429900585909
1201	24	0.808595654355166
1201	25	0.7041335443463481
1202	1	-0.18499537438220415
1202	2	0.03445144058136407
1202	3	-0.07147740364160882
1202	4	-0.03023616128933071
1202	5	0.011051273249705673
1202	6	-0.050843490739715946
1202	7	-0.032285413450146905
1202	8	0.08647421473253303
1202	9	0.09029136446039132
1202	10	0.37314146135535964
1202	11	0.13069341433836001
1202	12	0.07087966662517431
1202	13	0.1740495617874771
1202	14	0.385011450588415
1202	15	0.45055428169710154
1202	16	0.362893036530366
1202	17	0.2677870257852817
1202	18	0.2484808741634552
1202	19	0.32176781550437283
1202	20	0.5298409940799615
1202	21	0.4177497647461408
1202	22	0.35248804013161106
1202	23	0.6397872279497676
1202	24	0.3749536037056629
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2211	6	0.14067732238386554
2211	7	0.18352739176949484
2211	8	0.2477350927734534
2211	9	0.5083134957101194
2211	10	0.3550955198301864
2211	11	0.16494978376540104
2211	12	0.22302158371440584
2211	13	0.13120212394255049
2211	14	0.4104970063122195
2211	15	0.20138766809078845
2211	16	0.154904876390475
2211	17	0.2571482201729012
2211	18	0.14139495402650937
2211	19	0.2862414220369871
2211	20	0.2883083293248987
2211	21	0.2685910165566554
2211	22	0.31183817478005693
2211	23	0.481139630192537
2211	24	0.4341809593757091
2211	25	0.509051033085143
2212	1	-0.40918393795488583
2212	2	-0.3420691884172804
2212	3	-0.3031814808625093
2212	4	-0.7413475379646997
2212	5	-0.34964402577725906
2212	6	-0.7028265853081166
2212	7	-0.5552264921915651
2212	8	-0.37511316643151105
2212	9	-0.28655699673289803
2212	10	-0.6570847508078117
2212	11	-0.5819289775858962
2212	12	-0.7828068153447916
2212	13	-0.7556029344355153
2212	14	-0.7247157598696319
2212	15	-0.8003074581049887
2212	16	-0.5722340602532299
2212	17	-0.6611439971390709
2212	18	-0.47500296608993153
2212	19	-0.6938051960428098
2212	20	-0.7071652871622902
2212	21	-0.598467382608014
2212	22	-0.7246724303057448
2212	23	-0.15786396636067934
2212	24	-0.3859429476834464
2212	25	-1.1482743547836094
2213	1	-0.1711510293813383
2213	2	-1.1820913748991977
2213	3	-0.29113009730030015
2213	4	-0.021313227271470225
2213	5	-0.013976080707358562
2213	6	-0.024172702673780577
2213	7	0.01570451527265959
2213	8	0.1536664121078104
2213	9	0.07607808100226521
2213	10	-0.40555874973559725
2213	11	-0.1020527765054382
2213	12	-0.05627648419712017
2213	13	-0.3321172649233759
2213	14	0.021397234480652116
2213	15	-0.01918617475309503
2213	16	-0.05378785628605698
2213	17	0.08648353389313712
2213	18	0.09831873789786272
2213	19	-0.041843053274035924
2213	20	0.09576967922807
2213	21	-0.5056167908310343
2213	22	-0.8797410929310061
2213	23	-0.15873806426162673
2213	24	0.01810810596825263
2213	25	-0.613486693436748
2214	1	-0.19352845040012667
2214	2	-0.406066497554729
2214	3	-0.3794678116088974
2214	4	-0.5737894793235796
2214	5	-0.36662745400177954
2214	6	-0.2839932711170974
2214	7	-0.4682978000492142
2214	8	-0.11218877310616193
2214	9	-0.024765374503751048
2214	10	-1.9328105945737022
2214	11	-0.8449500614784569
2214	12	-0.49247912079051304
2214	13	-0.25481024570596844
2214	14	-0.8340319362195743
2214	15	-0.5833594541015046
2214	16	-0.8179667296217019
2214	17	-0.5752376358743589
2214	18	-0.6858909671400255
2214	19	-1.5506896202146336
2214	20	-2.6090811320767244
2214	21	-1.840631853377701
2214	22	-2.168159066235326
2214	23	-2.5582058699818617
2214	24	-1.6783744537460947
2214	25	-1.8400545408469142
2215	1	-0.26123250726636726
2215	2	-0.3573230944931147
2215	3	-0.8582004045499225
2215	4	-0.2680155719436783
2215	5	-0.23002981167398673
2215	6	-0.16220109512453007
2215	7	-0.29696716569904424
2215	8	-0.22251234731800906
2215	9	-0.2655690413956009
2215	10	-0.35279722456029816
2215	11	-0.6511746330783907
2215	12	-0.5692998248544114
2215	13	-0.357782752990623
2215	14	-0.22806412089953199
2215	15	-0.687484913587717
2215	16	-0.41936502711713786
2215	17	-0.2920113699673479
2215	18	-0.25124785936950644
2215	19	-2.1673011206453054
2215	20	-2.4704555841280893
2215	21	-3.3363970254719817
2215	22	-2.376204563206805
2215	23	-3.6558715715046617
2215	24	-2.506539571441138
2215	25	-1.8812245689038676
2216	1	-1.6043410200211703
2216	2	-1.2656676713307817
2216	3	-0.8039537684920859
2216	4	-1.0759207055815523
2216	5	-1.9719503827937856
2216	6	-1.6324829469028899
2216	7	-1.2093031465050004
2216	8	-5.444362902579904
2216	9	-4.24430345822984
2216	10	-2.2536014855056195
2216	11	-1.2656492322328106
2216	12	-1.2975157689502836
2216	13	-1.9291334954394586
2216	14	-3.5414072390289704
2216	15	-2.0006302486188545
2216	16	-3.1891931210860918
2216	17	-2.7939414024777847
2216	18	-2.8410120551151508
2216	19	-1.7080548213494353
2216	20	-0.879531758972341
2216	21	-0.7291948998005395
2216	22	-0.7429556358497607
2216	23	-1.0042368477166121
2216	24	-0.6475095370821506
2216	25	-0.44221780620358686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	152.0
8	708.0
9	1695.0
10	3223.0
11	5560.0
12	8662.0
13	12692.0
14	17064.0
15	22894.0
16	27890.0
17	31328.0
18	32952.0
19	33724.0
20	35366.0
21	37766.0
22	39795.0
23	41561.0
24	44595.0
25	47588.0
26	50399.0
27	55975.0
28	62762.0
29	73205.0
30	87220.0
31	106170.0
32	140997.0
33	196558.0
34	280263.0
35	433731.0
36	732800.0
37	2114701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.116181734663876	19.182170992810512	11.180463345753536	24.521183926772075
2	12.992513641993908	22.724611663486325	36.32283401961769	27.960040674902082
3	21.440372650793048	28.06318089808975	26.918294949369827	23.578151501747378
4	13.42925373459121	18.900286263035994	32.58389373387141	35.08656626850138
5	12.22299500799917	39.64238291981622	34.39764347765317	13.736978594531438
6	33.46478545561665	32.625734697690454	17.782294112564276	16.127185734128616
7	24.865983263598327	33.32541841004184	22.393138075313807	19.415460251046024
8	27.237219363659094	35.69243418127881	20.98115370654929	16.089192748512808
9	26.76912429978962	17.432016622621962	20.084259555246117	35.7145995223423
10	16.711370320943715	31.462132716819696	31.46920385096476	20.357293111271826
11	30.137141056772542	27.18708008357376	19.204915345685297	23.470863513968396
12	23.256422599617526	30.60351798143791	28.317304946467093	17.822754472477474
13	28.72163487870672	22.315583039150173	22.87628491128061	26.0864971708625
14	21.48622704306689	25.850656791038794	25.38850033567065	27.274615830223663
15	30.722030029004717	25.072114298338548	21.09480258213959	23.111053090517146
16	20.077101983031874	28.776190646546596	29.964331912174785	21.182375458246746
17	25.506653070839587	29.656637024472072	26.741093638399814	18.095616266288527
18	25.78632917790462	26.198678140191117	27.888869548771822	20.126123133132438
19	23.248532633527418	30.679800300921006	28.325256611308134	17.74641045424344
20	23.9388248998323	27.313480547215217	26.749273637736025	21.998420915216453
21	20.049632498817502	29.586132716682062	29.225823891314374	21.138410893186062
22	22.110471749331097	28.874062339762613	22.800069596285017	26.21539631462127
23	18.80382556726484	29.79027134409084	27.12578275555079	24.280120333093528
24	21.476043354265133	33.80807151847042	24.21129179107475	20.504593336189696
25	22.698722509014186	26.26650958280733	22.251079637029772	28.783688271148716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14665.0
1	14665.0
2	17026.5
3	19388.0
4	19388.0
5	19388.0
6	22240.0
7	25092.0
8	25092.0
9	25092.0
10	25306.5
11	25521.0
12	25521.0
13	25521.0
14	30368.5
15	35216.0
16	35216.0
17	35216.0
18	47047.0
19	58878.0
20	58878.0
21	58878.0
22	79544.5
23	100211.0
24	100211.0
25	100211.0
26	129012.5
27	157814.0
28	157814.0
29	157814.0
30	191789.5
31	225765.0
32	225765.0
33	225765.0
34	260023.5
35	294282.0
36	294282.0
37	294282.0
38	330122.5
39	365963.0
40	365963.0
41	365963.0
42	429679.5
43	493396.0
44	493396.0
45	493396.0
46	809314.5
47	1125233.0
48	1125233.0
49	1125233.0
50	977873.0
51	830513.0
52	830513.0
53	830513.0
54	618064.5
55	405616.0
56	405616.0
57	405616.0
58	323221.0
59	240826.0
60	240826.0
61	240826.0
62	203581.5
63	166337.0
64	166337.0
65	166337.0
66	133374.5
67	100412.0
68	100412.0
69	100412.0
70	76728.0
71	53044.0
72	53044.0
73	53044.0
74	39096.5
75	25149.0
76	25149.0
77	25149.0
78	17790.0
79	10431.0
80	10431.0
81	10431.0
82	7292.0
83	4153.0
84	4153.0
85	4153.0
86	2812.0
87	1471.0
88	1471.0
89	1471.0
90	975.0
91	479.0
92	479.0
93	479.0
94	293.5
95	108.0
96	108.0
97	108.0
98	74.0
99	40.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031338892465130254
2	0.013451874402589287
3	0.017510449261224314
4	0.017405846816414133
5	0.015836810144261415
6	0.006610874512003444
7	6.276146688610865E-5
8	3.765688013166519E-4
9	2.3012537858239836E-4
10	1.255229337722173E-4
11	7.112966247092313E-4
12	9.62342492253666E-4
13	0.0021129693851656577
14	4.184097792407243E-4
15	0.0019037644955452956
16	0.0020920488962036217
17	0.0021966513410138027
18	0.0014853547163045714
19	2.3012537858239836E-4
20	3.9748929027868813E-4
21	0.08577400474434849
22	0.08129702010647273
23	0.08215476015391622
24	7.322171136712676E-4
25	0.221255091262495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4780003.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.156137687734315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26719831520249	41.763610635691755
2	10.791746007745697	10.825445973111007
3	2.959661979460734	4.453356412529546
4	1.1289617620238617	2.264974463210238
5	0.5534995289710961	1.388069929258519
6	0.3143486579328896	0.9459908741541904
7	0.20007498772443055	0.7024492042524804
8	0.12800481643785339	0.5136181758360105
9	0.09726649198532239	0.43906604079768624
>10	0.4945465981342858	4.444975388339186
>50	0.03396936093617539	1.1673214942615548
>100	0.022908530222809146	2.2974191069836816
>500	0.002745095210635868	0.9553319693196125
>1k	0.003336346440995903	3.707749647286317
>5k	3.378578674426228E-4	1.25488861161426
>10k+	0.001393663703200819	22.875732073354033
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	164144	3.4339727401844726	No Hit
GTACATGGGAAGCAGTGGTATCAAC	163590	3.4223827892995042	No Hit
GAGTACATGGGAAGCAGTGGTATCA	77402	1.6192876866395274	No Hit
CATGTACTCTGCGTTGATACCACTG	65153	1.3630326173435456	No Hit
GCGTTGATACCACTGCTTCCCATGT	53626	1.121882141078154	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	43472	0.9094554961576384	No Hit
GCTTCCCATGTACTCTGCGTTGATA	41857	0.87566890648395	No Hit
GTATCAACGCAGAGTACATGGGAAG	40534	0.847991099587176	No Hit
ACTCTGCGTTGATACCACTGCTTCC	36776	0.7693719020678439	No Hit
TATCAACGCAGAGTACATGGGAAGC	33173	0.6939953803376274	No Hit
GGTATCAACGCAGAGTACATGGGAA	27596	0.5773218133963514	No Hit
CAGTGGTATCAACGCAGAGTACATG	23224	0.48585743565432904	No Hit
GTGGTATCAACGCAGAGTACATGGG	21045	0.44027169020605217	No Hit
GCAGTGGTATCAACGCAGAGTACAT	18845	0.3942466144895725	No Hit
ATACCACTGCTTCCCATGTACTCTG	18567	0.38843071855812644	No Hit
GTACATGGTAAGCAGTGGTATCAAC	18458	0.38615038526126444	No Hit
GTACTCTGCGTTGATACCACTGCTT	17166	0.3591211135223137	No Hit
CATGGGAAGCAGTGGTATCAACGCA	16120	0.3372382820680238	No Hit
ACATGGGAAGCAGTGGTATCAACGC	15972	0.33414204970164246	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	15946	0.3335981169886295	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	15050	0.314853358878645	No Hit
TACCACTGCTTCCCATGTACTCTGC	14344	0.3000834936714475	No Hit
ACCATGTACTCTGCGTTGATACCAC	13660	0.28577387922141473	No Hit
CTTCCCATGTACTCTGCGTTGATAC	13578	0.28405839912652775	No Hit
GTTGATACCACTGCTTCCCATGTAC	13491	0.2822383165868306	No Hit
GATACCACTGCTTCCCATGTACTCT	13393	0.28018810866855104	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	13100	0.27405840540267445	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	13041	0.27282409655391426	No Hit
CCACTGCTTCCCATGTACTCTGCGT	12672	0.2651044361269229	No Hit
CCATGTACTCTGCGTTGATACCACT	12507	0.26165255544818694	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	11539	0.24140152213293592	No Hit
TTCCCATGTACTCTGCGTTGATACC	10545	0.22060655610467192	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10372	0.2169873115142396	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9824	0.20552288356304377	No Hit
TGATACCACTGCTTCCCATGTACTC	9411	0.19688272162172285	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7954	0.16640156920403606	No Hit
CCCCATGTACTCTGCGTTGATACCA	6994	0.1463178998004813	No Hit
ATCAACGCAGAGTACATGGGAAGCA	6677	0.13968610479951582	No Hit
GCTTACCATGTACTCTGCGTTGATA	6636	0.13882836475207233	No Hit
GAGTACATGGTAAGCAGTGGTATCA	6228	0.13029280525556156	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5519	0.11546017858147788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.1840977924072435E-5	0.0
5	0.0	0.0	0.0	4.1840977924072435E-5	0.0
6	0.0	0.0	0.0	4.1840977924072435E-5	0.0
7	0.0	0.0	0.0	4.1840977924072435E-5	0.0
8	0.0	0.0	0.0	6.276146688610865E-5	0.0
9	0.0	0.0	0.0	6.276146688610865E-5	0.0
10	0.0	0.0	0.0	8.368195584814487E-5	0.0
11	0.0	0.0	0.0	8.368195584814487E-5	0.0
12	0.0	0.0	0.0	1.464434227342535E-4	0.0
13	0.0	0.0	0.0	1.464434227342535E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAT	260	0.0	11.328489	1
GAACAAA	2095	0.0	11.202025	1
GTATACG	60	0.005872637	11.084866	1
CCTACAC	470	0.0	10.510112	3
GTATAGG	415	0.0	10.073682	1
TATCGAC	190	9.640644E-11	10.02026	19
CGCAAAA	440	0.0	9.71593	2
GTATCGA	210	6.548362E-11	9.504795	18
GTCCTAA	280	0.0	9.501313	1
TAGGACC	180	4.1964086E-9	9.499226	4
ACGCAAA	555	0.0	9.415716	1
GTGTAGC	405	0.0	9.384013	1
CCTAGCC	235	7.2759576E-12	9.297407	3
TACGTGG	310	0.0	9.193569	2
GTACTAG	230	4.1836756E-11	9.088213	1
AGGACCG	115	1.1133203E-4	9.085646	5
GGTATCG	210	6.6211214E-10	9.052186	17
CTACACT	580	0.0	9.007887	4
TAGACTG	380	0.0	8.998701	5
CCGTGCG	85	0.007464914	8.937455	9
>>END_MODULE