FastQCFastQC Report
Wed 25 May 2016
SRR1294729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780003
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1791783.748491371239725No Hit
GTACATGGGAAGCAGTGGTATCAAC1645903.4433032782615407No Hit
GAGTACATGGGAAGCAGTGGTATCA859961.7990783687792662No Hit
CATGTACTCTGCGTTGATACCACTG749301.5675722379253736No Hit
GCGTTGATACCACTGCTTCCCATGT584781.2233883535219539No Hit
ACGCAGAGTACATGGGAAGCAGTGG534991.1192252389799755No Hit
GCTTCCCATGTACTCTGCGTTGATA469760.9827608894806134No Hit
GTATCAACGCAGAGTACATGGGAAG443940.9287441869806358No Hit
ACTCTGCGTTGATACCACTGCTTCC422760.8844345913590431No Hit
TATCAACGCAGAGTACATGGGAAGC396940.8304178888590656No Hit
GGTATCAACGCAGAGTACATGGGAA312290.653325949795429No Hit
CAGTGGTATCAACGCAGAGTACATG283530.5931586235406129No Hit
GTGGTATCAACGCAGAGTACATGGG237650.4971754201827907No Hit
GCAGTGGTATCAACGCAGAGTACAT218650.45742649115492184No Hit
CATGGGAAGCAGTGGTATCAACGCA212100.4437235708847882No Hit
ATACCACTGCTTCCCATGTACTCTG209600.4384934486442791No Hit
ACATGGGAAGCAGTGGTATCAACGC203440.42560642744366484No Hit
GGGAAGCAGTGGTATCAACGCAGAG179740.37602486860363893No Hit
GTACATGGTAAGCAGTGGTATCAAC178670.37378637628470107No Hit
CTGCTTCCCATGTACTCTGCGTTGA175800.3677821959525967No Hit
GTACTCTGCGTTGATACCACTGCTT173680.363347052292645No Hit
AAGCAGTGGTATCAACGCAGAGTAC165350.34592028498726884No Hit
CTTCCCATGTACTCTGCGTTGATAC161560.3379914196706571No Hit
ACCATGTACTCTGCGTTGATACCAC158670.33194539836062864No Hit
TACCACTGCTTCCCATGTACTCTGC156310.3270081629655881No Hit
GATACCACTGCTTCCCATGTACTCT155460.325229921403815No Hit
GCAGAGTACATGGGAAGCAGTGGTA152480.31899561569312823No Hit
CCATGTACTCTGCGTTGATACCACT149320.3123847411811248No Hit
GTTGATACCACTGCTTCCCATGTAC148460.3105855791303897No Hit
CCACTGCTTCCCATGTACTCTGCGT142620.2983680135765605No Hit
ATGGGAAGCAGTGGTATCAACGCAG130750.2735353931786235No Hit
GTATCAACGCAGAGTACTTTTTTTT113430.2373011062963768No Hit
TTCCCATGTACTCTGCGTTGATACC111440.2331379289929316No Hit
GTACATGGGTAAGCAGTGGTATCAA107180.22422580069510417No Hit
TGATACCACTGCTTCCCATGTACTC102400.21422580697125085No Hit
GGTAAGCAGTGGTATCAACGCAGAG95580.19995803349914218No Hit
TATCAACGCAGAGTACTTTTTTTTT88190.18449779215619738No Hit
GGTATCAACGCAGAGTACTTTTTTT85340.17853545280201708No Hit
ATCAACGCAGAGTACATGGGAAGCA80520.1684517771223156No Hit
CCCCATGTACTCTGCGTTGATACCA77200.1615061747869196No Hit
GCTTACCATGTACTCTGCGTTGATA75780.15853546535431043No Hit
GAGTACATGGTAAGCAGTGGTATCA70020.1464852637121776No Hit
GTACTTTTTTTTTTTTTTTTTTTTT65800.1376568173701983No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64350.13462334647070306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA60010.12554385426117934No Hit
GGAAGCAGTGGTATCAACGCAGAGT57310.11989532224142956No Hit
ACTCTGCGTTGATACCACTGCTTAC52580.10999993096238643No Hit
AAAAAGTACTCTGCGTTGATACCAC51710.10817984842268927No Hit
GCGTTGATACCACTGCTTACCATGT48740.10196646320096452No Hit
ATGGTAAGCAGTGGTATCAACGCAG47960.10033466506192568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA500.001506411713.29287612
TACACCG951.3418272E-511.0146155
CAGTGCG2001.8189894E-1210.4444039
CTAGAAC3400.010.3519723
TACGCAG1409.518044E-810.1920955
GTACCGT750.002659246610.1287536
AGTGCGA750.002661093110.12790510
CTACGCA951.6235441E-410.0132874
ACCGCAT1151.0341319E-59.9077348
TCTACGC1252.5298668E-69.8931273
TAGCCTG3200.09.5126235
GTTCTAG2650.09.3376411
GTCTTAG2451.8189894E-129.3229821
AGTGCGG1752.6977432E-89.22362810
GTATTAA4850.09.0266331
TGGACCG950.00180298359.0119585
AGTACTC15950.09.0056815
GTCTAAT2553.6379788E-128.9573751
GTTCGGG850.007329838.9573751
CTTACTC4500.08.87844853
Produced by FastQC (version 0.11.5)